Utilizing liquid-liquid biopolymer regulators to predict the prognosis and drug sensitivity of hepatocellular carcinoma by Jianhao Li, Han Chen, Lang Bai, Hong Tang

“…Abstract Background Liquid-liquid phase separation (LLPS) is essential for the formation of membraneless organelles and significantly influences cellular compartmentalization, chromatin remodeling, and gene regulation. …”
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Candida albicans Fungaemia following Traumatic Urethral Catheterisation in a Paraplegic Patient with Diabetes Mellitus and Candiduria Treated by Caspofungin by Subramanian Vaidyanathan, Bakul Soni, Peter Hughes, Gordon Ramage, Leighann Sherry, Gurpreet Singh, Paul Mansour

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Ascorbic acid-containing compound efficacy in ischemic brain damage by Rita M. Termulaeva, Konstantin Y. Belanov, Natalya D. Bunyatyan, Aleksander S. Pirozhkov, Dmitrii E. Timoshkin, Ekaterina V. Blinova, Olga V. Vasilkina, Kirill D. Blinov, Elena V. Semeleva, Aleksander A. Dmitriev, Dmitrii S. Blinov

“…The aim: to assess anti-ischemic activity of 3-hydroxypyridine ascorbate in the in vitro and in vivo models of brain cell and tissue response to ischemia and reoxygenation. …”
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Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling by Walid Mottawea, Basit Yousuf, Salma Sultan, Tamer Ahmed, JuDong Yeo, Nico Hüttmann, Yingxi Li, Nour Elhouda Bouhlel, Hebatoallah Hassan, Xu Zhang, Zoran Minic, Riadh Hammami

“…We hypothesize that MEVs cross host cellular barriers and deliver their cargoes of bioactive compounds to the brain. …”
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High-accuracy crRNA array assembly strategy for multiplex CRISPR by Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang

“…Simultaneous targeting of multiple loci with the CRISPR system, a tool known as multiplex CRISPR, offers greater feasibility for manipulating and elucidating the intricate and redundant endogenous networks underlying complex cellular functions. Owing to the versatility of continuously emerging Cas nucleases and the use of CRISPR arrays, multiplex CRISPR has been implemented in numerous in vitro and in vivo studies. …”
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Cannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome via CB2 Receptor Activation and TRPV1 Desensitization by Jun Jie Piao, Soomin Kim, Dongho Shin, Hwa Jong Lee, Kyung-Hwa Jeon, Wen Jie Tian, Kyung Jae Hur, Jong Soo Kang, Hyun-Je Park, Joo Young Cha, Aeri Song, Sang-Hyuck Park, Mahadevan Rajasekaran, Woong Jin Bae, Sungjoo Kim Yoon, Sae Woong Kim

“…Enzyme-linked immunosorbent assays and western blots were performed on cellular components and supernatants after administration of CBD. …”

Combining single-cell analysis and molecular docking techniques to construct a prognostic model for colon adenocarcinoma and uncovering inhibin subunit βb as a novel therapeutic ta... by Qinqing Wu, Qinqing Wu, Lu Ye, Yuwei Wu, Mengyu Zhao, Mengyu Zhao, Jiaxin Lu, Yanping Yu, Yanping Yu, Yixiao Niu, Yixiao Niu, Luxiao Zhang, Peijun Zuo, Peijun Zuo

“…Molecular docking identified potential drug candidates with high binding affinity to INHBB, including risperidone. Furthermore, in vitro experiments with INHBB showed that downregulation of its expression in COAD cell lines significantly reduced cellular viability and migration capacity.ConclusionIn summary, our research, based on the expression characteristics of ARGs, provides new insights into the precise classification, prognosis assessment, and identification of potential therapeutic targets in COAD. …”
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Experimental use of mycobacterial antigens in tuberculin skin test on water buffalo by Alessandra Martucciello, Federica Signorelli, Piera Mazzone, Francesco Grandoni, Giovanna Cappelli, Lucia Boccia, Giuseppe Parente, Monica Cagiola, Maria Beatrice Boniotti, Esterina De Carlo, Francesco Napolitano, Giorgio Galiero

“…All subjects were also submitted to the IFN-γ test using the same antigens (Ag) as the TST to verify the cellular response in vitro and to exclude anaergic animals that did not respond to in vitro stimulation with the mitogen. …”
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Personalized allele-specific antisense oligonucleotides for GNAO1-neurodevelopmental disorder by Inna Shomer, Nofar Mor, Shaul Raviv, Noga Budick-Harmelin, Tanya Matchevich, Sharon Avkin-Nachum, Yoach Rais, Rebecca Haffner-Krausz, Ariela Haimovich, Aviv Ziv, Reut Fluss, Bruria Ben-Ze’ev, Gali Heimer, Denis N. Silachev, Vladimir L. Katanaev, Dan Dominissini

“…We systemically screened allele-specific antisense oligonucleotides (ASOs) targeting the mutated allele to identify a potent and specific sequence using both reporter-based platforms and a patient-derived cellular model. We show that reduction of mutated GNAO1 in vitro by knockout or by ASO has a beneficial functional outcome, which can be measured by cAMP accumulation and gene expression changes. …”
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Moscatilin inhibits vascular calcification by activating IL13RA2-dependent inhibition of STAT3 and attenuating the WNT3/β-catenin signalling pathway by Tingting Zhang, Mengmeng Zhu, Jialing Ma, Zhenghong Liu, Zhidan Zhang, Meijie Chen, Yaping Zhao, Huaxin Li, Shengnan Wang, Xiaoning Wei, Wenwen Zhang, Xiaoxiao Yang, Peter J. Little, Danielle Kamato, Hao Hu, Yajun Duan, Baotong Zhang, Jianbo Xiao, Suowen Xu, Yuanli Chen

“…In vitro, we established the cellular model of osteogenesis of human aortic smooth muscle cells (HASMCs) under phosphate conditions. …”
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Astaxanthin-loaded brain-permeable liposomes for Parkinson’s disease treatment via antioxidant and anti-inflammatory responses by Thai-Duong Nguyen, Shristi Khanal, Eunhee Lee, Jinsol Choi, Ganesh Bohara, Nikesh Rimal, Dong-Young Choi, Soyeun Park

“…Lf-functionalized liposomes demonstrated enhanced cellular uptake and permeation in a Transwell® study, showing a 16.7-fold higher internalization in SH-SY5Y cells over 24 h than those without Lf conjugation. …”
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Non-invasive imaging with ICOS-targeting monoclonal antibody for preclinical diagnosis of rheumatoid arthritis in a humanized mouse model by Shao Duan, Chao Li, Feng Yan, Yifei Xia, Shuaiming Shao, Weiyu Chen, Zunyu Xiao, Gongping Xu

“…Findings ICOS was confirmed as an indicator of RA pathogenesis via RNA-seq, flow cytometry and immunofluorescence data. An in-vitro cellular uptake study validated the specificity of Cy7-ICOS mAb to activated T cells. …”
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Efficacy of Air and Oxygen Micro-nano Bubble Waters Against Colletotrichum gloeosporioides and Impacts on Postharvest Quality of ‘Fan Retief’ Guava Fruit by Harold K. Malahlela, Zinash A. Belay, Rebogile R. Mphahlele, Oluwafemi James Caleb

“…Air- and O2-MNB treatments damaged cellular structures, disrupted cell membrane integrity, and deformed hyphal morphology. …”
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Preventive Effect of Cocoa Flavonoids via Suppression of Oxidative Stress-Induced Apoptosis in Auditory Senescent Cells by Luz del Mar Rivas-Chacón, Joaquín Yanes-Díaz, Beatriz de Lucas, Juan Ignacio Riestra-Ayora, Raquel Madrid-García, Ricardo Sanz-Fernández, Carolina Sánchez-Rodríguez

“…The objective is to determine the protective effect of cocoa at the cellular and molecular levels in Presbycusis. For in vitro study, we used House Ear Institute-Organ of Corti 1 (HEI-OC1), stria vascularis (SV-k1), and organ of Corti (OC-k3) cells (derived from the auditory organ of a transgenic mouse). …”
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Cromolyn sodium reduces LPS-induced pulmonary fibrosis by inhibiting the EMT process enhanced by MC-derived IL-13 by Cheng Tan, Hang Zhou, Qiangfei Xiong, Xian Xian, Qiyuan Liu, Zexin Zhang, Jingjing Xu, Hao Yao

“…We investigated the level of mast cell and epithelial-mesenchymal transition(EMT) in LPS-induced mouse model and cellular model. We also explored the influence of cromolyn sodium and mast cell knockout on pulmonary fibrosis. …”
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Downregulation of TNF-α/TNF-R1 Signals by AT-Lipoxin A4 May Be a Significant Mechanism of Attenuation in SAP-Associated Lung Injury by Suhui Yu, Jianming Xie, Yukai Xiang, Shengjie Dai, Dinglai Yu, Hongwei Sun, Bicheng Chen, Mengtao Zhou

“…We used western blot, polymerase chain reaction (PCR), and immunofluorescence to investigate the downregulation of TNF-α signals in cellular and animal models of SAP-associated lung injury following AT-Lipoxin A4 intervention. …”
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Integrative analysis of anoikis-related prognostic signature to evaluate the immune landscape and predict therapeutic response in stomach adenocarcinoma by Ziyi Zhou, Lanlan Yang, Yuan Fang, Rongzhong Xu, Xi Wang, Yuli Wang, Zhihong Fang

“…Finally, in vitro experiments confirmed the critical role of CRABP2 in STAD. …”
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Functionalization of collagen fiber with nano-islands of silver via atomic layer deposition to promote bone healing by Sarah Hashemi Astaneh, Leonardo P. Faverani, Harshdeep Bhatia, Eduardo Dallazen, Monique Gonçalves Costa, Edilson Ervolino, Valentim A.R. Barão, Cortino Sukotjo, Christos G. Takoudis

“…This novel solvent-free method can be used to functionalize sensitive materials used in surgeries as bone grafting agents to enhance osteopromotive properties without any adverse effects to the cellular environment.…”
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Reversal gene expression assessment for drug repurposing, a case study of glioblastoma by Shixue Sun, Zeenat Shyr, Kathleen McDaniel, Yuhong Fang, Dingyin Tao, Catherine Z. Chen, Wei Zheng, Qian Zhu

“…Among five prioritized candidates, in-vitro experiments validated Clofarabine and Ciclopirox as highly efficacious in selectively targeting GBM cancer cells. …”
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Discovery of orally bioavailable SARS-CoV-2 papain-like protease inhibitor as a potential treatment for COVID-19 by Yongzhi Lu, Qi Yang, Ting Ran, Guihua Zhang, Wenqi Li, Peiqi Zhou, Jielin Tang, Minxian Dai, Jinpeng Zhong, Hua Chen, Pan He, Anqi Zhou, Bao Xue, Jiayi Chen, Jiyun Zhang, Sidi Yang, Kunzhong Wu, Xinyu Wu, Miru Tang, Wei K. Zhang, Deyin Guo, Xinwen Chen, Hongming Chen, Jinsai Shang

“…The lead compound GZNL-P36 not only inhibits SARS-CoV-2 and its variants at the cellular level with EC50 ranging from 58.2 nM to 306.2 nM, but also inhibits HCoV-NL63 and HCoV-229E with EC50 of 81.6 nM and 2.66 μM, respectively. …”
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