Marek's disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius. by Chenyi Bao, Jun Chu, Qi Gao, Shasha Yang, Xiaoyu Gao, Wenwen Chen, Fuchun Yang, Fei Jiang, Chenxi Tong, Mingyi Lei, Linlin Jiao, Jitong Li, Kexin Wei, Xue Lian, Kai Li, Suresh Kumar Tikoo, Nikolaus Osterrieder, Lorne A Babiuk, Yufeng Li, Yong-Sam Jung, Yingjuan Qian

“…Furthermore, we showed that pLORF1 associated with the cellular membrane, interacted with MDV structural proteins, and exhibited punctate colocalization with tegument or capsid proteins in the cytoplasm. …”
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TransLeish: Identification of membrane transporters essential for survival of intracellular Leishmania parasites in a systematic gene deletion screen by Andreia Albuquerque-Wendt, Ciaran McCoy, Rachel Neish, Ulrich Dobramysl, Çağla Alagöz, Tom Beneke, Sally A. Cowley, Kathryn Crouch, Richard J. Wheeler, Jeremy C. Mottram, Eva Gluenz

“…Abstract For the protozoan parasite Leishmania, completion of its life cycle requires sequential adaptation of cellular physiology and nutrient scavenging mechanisms to the different environments of a sand fly alimentary tract and the acidic mammalian host cell phagolysosome. …”
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Regulation of Hypoxia-Induced Cell Death in Human Tenocytes by Min Liang, Hannah R. Cornell, Nasim Zargar Baboldashti, Mark S. Thompson, Andrew J. Carr, Philippa A. Hulley

“…We find that tenocytes respond to hypoxia in vitro by activating classical HIF-1α-driven pathways. …”
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Lysophosphatidic Acid Induced Apoptosis, DNA Damage, and Oxidative Stress in Spinal Cord Neurons by Upregulating LPA4/LPA6 Receptors by Yifan Yang, Jing Xu, Qingxin Su, Yiran Wu, Qizheng Li, Zongren Ma, Tao Ding

“…In spinal cord neurons treated with LPA (18 : 1), cellular activity decreased and LDH release increased. …”
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Pretreatment with serine protease inhibitors impairs Leishmania amazonensis survival on macrophages by Patrícia de Almeida Machado, Pollyanna Stephanie Gomes, Elaine Soares Coimbra, Herbert Leonel de Matos Guedes

“…The discovery of new therapeutic alternatives is extremely essential, and the discovery of cellular targets is a tool that helps in the development of new drugs. …”
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Decellularized Human Stromal Lenticules Combine with Corneal Epithelial-Like Cells: A New Resource for Corneal Tissue Engineering by Shuai Qin, Shuai Zheng, Bing Qi, Rui Guo, Guanghui Hou

“…After inducing differentiation in vitro, corneal epithelial-like cells were induced. …”
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NAT10 promotes ovarian cancer cell migration, invasion, and stemness via N4-acetylcytidine modification of CAPRIN1 by Yang Song, Min Cheng

“…In conclusion, NAT10 promotes OC progression by promoting cellular migration, invasion, and stemness via upregulating CAPRIN1 expression. …”
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The Manufacture of GMP-Grade Bone Marrow Stromal Cells with Validated In Vivo Bone-Forming Potential in an Orthopedic Clinical Center in Brazil by Rhayra B. Dias, João A. M. Guimarães, Marco B. Cury, Leonardo R. Rocha, Elaine S. da Costa, Liebert P. Nogueira, Camila Hochman-Mendez, Anneliese Fortuna-Costa, Anna Karoline F. Silva, Karin S. Cunha, Sergio A. L. de Souza, Maria Eugênia L. Duarte, Rafaela C. Sartore, Danielle C. Bonfim

“…In vitro-expanded bone marrow stromal cells (BMSCs) have long been proposed for the treatment of complex bone-related injuries because of their inherent potential to differentiate into multiple skeletal cell types, modulate inflammatory responses, and support angiogenesis. …”
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Interleukin-1 Receptor-Associated Kinase-3 Aggravates Neuroinflammatory Injury After Intracerebral Hemorrhage via Activation NF-κB/IL-17A Pathway in Mice by Wang J, Li Y, Tan C, Shao J, Tang W, Kong Q, Sheng W, Ding Z, Li F, Piao J, Lv D, Hu L, Wang Q, Jiang X

“…Immunofluorescence was used to investigate the cellular localization of IRAK3. The ICH model was treated with recombinant human IRAK3 (rh-IRAK3) or IRAK3 siRNA via an intracerebroventricular injection. …”
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Assessing gut barrier integrity and reproductive performance following pre-mating oral administration of solid-lipid-nanoparticles designed for drug delivery by Valentina Lacconi, Valentina Lacconi, Micol Massimiani, Micol Massimiani, Giulia Antonello, Paolo Gasco, Roberta Bernardini, Cristiana Ferrari, Lorenzo Ippoliti, Gina La Sala, Antonio Pietroiusti, Ivana Fenoglio, Chiara Riganti, Luisa Campagnolo

“…Solid lipid nanoparticles (SLNs) have gained interest as drug delivery carriers due to their efficient cellular internalization and increased therapeutic effect of the loaded drug, with minimal side effects. …”
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Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling by Bisha Ding, Jie Li, Jia-Lin Yan, Chun-Yan Jiang, Ling-Bo Qian, Jie Pan

“…The JNK inhibitor sp600125 was utilized to elucidate the specific role of JNK in modulating ULBP2 expression.ResultsRES increased ULBP2 expression on BC cells, thereby augmenting their vulnerability to NK cell-mediated cytotoxicity both in vitro and in vivo. RES administration led to a reduction in cellular miR-17-5p level. …”
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Comparison of human-induced pluripotent stem cell-derived macrophages with peripheral blood-derived macrophages using single-cell genomics by ZHANG Yutong, HOU Guojun, SHEN Nan

“…Objective·To explore the heterogeneity in single-cell genomics between human-induced pluripotent stem cell (iPSC)-derived macrophages (IPSDM) and human peripheral blood-derived macrophages (PBDM).Methods·iPSCs were differentiated into IPSDMs in vitro using a feeder-free and serum-free protocol. …”
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CD21low B cells reveal a unique glycosylation pattern with hypersialylation and hyperfucosylation by Peter Tobias Felixberger, Peter Tobias Felixberger, Geoffroy Andrieux, Andrea Maul-Pavicic, Andrea Maul-Pavicic, Sigune Goldacker, Sigune Goldacker, Ina Harder, Ina Harder, Sylvia Gutenberger, Sylvia Gutenberger, Jonathan J. M. Landry, Vladimir Benes, Till Fabian Jakob, Melanie Boerries, Melanie Boerries, Lars Nitschke, Reinhard Edmund Voll, Reinhard Edmund Voll, Klaus Warnatz, Klaus Warnatz, Baerbel Keller, Baerbel Keller

“…BackgroundThe posttranslational modification of cellular macromolecules by glycosylation is considered to contribute to disease pathogenesis in autoimmune and inflammatory conditions. …”
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ALDOA contributes to colorectal tumorigenesis and metastasis by targeting YAP by Liang Sun, Ting Lu, Linhua Jiang, Huihui Yao, Qixuan Xu, Jie Sun, Xiaoqin Yang, Songbing He, Xinguo Zhu

“…In addition, loss of function and gain of function experiments showed that ALDOA promoted CRC cell proliferation and migration in vitro and in vivo. Mechanistically, high ALDOA expression inhibited AMP-activated protein kinase (AMPK) phosphorylation possibly through regulating cellular glycolysis or the formation of v-ATPase-regulator-AXIN/LKB1 complex, which led to Yes-associated protein (YAP) unphosphorylation and enhanced the proliferative and migratory potential of CRC cells. …”
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Ion homeostasis in the regulation of intracellular pH and volume of human erythrocytes by О. І. Доценко, Г. В. Тарадіна

“…Objectives: Creation of a mathematical metabolic model of erythrocyte ion homeostasis to study the mechanisms of erythrocyte volume stabilization and intracellular pH in in vitro experiments. Material and Methods: The mathematical model was developed using general approaches to modeling cellular metabolism, which are based on systems of ordinary differential equations describing metabolic reactions, passive and active ion fluxes. …”
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A phase-transited lysozyme coating doped with strontium on titanium surface for bone repairing via enhanced osteogenesis and immunomodulatory by Yu Zhang, Yu Chen, Yidan Shi, Hongkun Hu, Zhongyu Dai, Zhichen Liu, Xuanan Li

“…A series of cell and animal experiments were conducted to investigate the biological functions of PTL-Sr coatings.ResultsThe characterization indicates the successful preparation of PTL-Sr coatings. In vitro cellular experiments have demonstrated that it promotes M2 macrophage polarization and reduces inflammatory mediator production while promoting osteogenic differentiation of bone merrow mesenchymal stem cells (BMSCs). …”
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Optimising metallic coatings strategies for enhanced surface performance of bioresorbable polymeric stents by Ana M. Sousa, Ana M. Amaro, Ana P. Piedade

“…However, challenges persist, including issues of cellular compatibility, mechanical strength, and degradation rates across stent types. …”
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Cell-free expression and SMA copolymer encapsulation of a functional receptor tyrosine kinase disease variant, FGFR3-TACC3 by Alexander J. D. Snow, Tharushi Wijesiriwardena, Benjamin J. Lane, Brendan Farrell, Polly C. Dowdle, Matilda Katan, Stephen P. Muench, Alexander L. Breeze

“…This platform is characterised by utilising an optimised solution of cellular machinery to facilitate protein synthesis in vitro. …”
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Inosine Prevents Colorectal Cancer Progression by Inducing M1 Phenotypic Polarization of Macrophages by Yuchen Ma, Xiaoli Qian, Qun Yu, Yadan Dong, Jiapeng Wang, Heng Liu, Huai Xiao

“…Aside from the already reported effects of inosine on T cells, in this study, in vitro experiments revealed that inosine could modulate macrophage phenotype. …”
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Rapamycin Improves Palmitate-Induced ER Stress/NF κ B Pathways Associated with Stimulating Autophagy in Adipocytes by Jiajing Yin, Liping Gu, Yufan Wang, Nengguang Fan, Yuhang Ma, Yongde Peng

“…Autophagy helps to adapt to cellular stress and involves in regulating innate inflammatory response. …”
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