Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss by Luz del Mar Rivas-Chacón, Sofía Martínez-Rodríguez, Raquel Madrid-García, Joaquín Yanes-Díaz, Juan Ignacio Riestra-Ayora, Ricardo Sanz-Fernández, Carolina Sánchez-Rodríguez

“…Hydrogen peroxide (H₂O₂) represents a method commonly used to generate cellular senescence in vitro. The objective of the present paper is to study H₂O₂-induced senescence patterns in three auditory cell lines (House Ear Institute-Organ of Corti 1, HEI-OC1; organ of Corti, OC-k3, and stria vascularis, SV-k1 cells) to elucidate the intrinsic mechanisms responsible for ARHL. …”
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“Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration by Oksana A. Zhukova, Iuliia V. Ozerskaya, Dmitry V. Basmanov, Vsevolod Yu. Stolyarov, Vladimir G. Bogush, Vladimir V. Kolesov, Kirill A. Zykov, Gaukhar M. Yusubalieva, Vladimir P. Baklaushev

“…Modern LoC modifications include the same elements and differ only by the construction of the microfluidic system, by the biomaterial of semipermeable membrane, by the composition of cellular and stromal elements and by specific tasks to be solved. …”
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Zinc regulates microglial polarization and inflammation through IKBα after spinal cord injury and promotes neuronal repair and motor function recovery in mice by Daoyong Li, Daoyong Li, Mingyu Bai, Mingyu Bai, Zhanpeng Guo, Yang Cui, Yang Cui, Xifan Mei, Xifan Mei, He Tian, He Tian, Zhaoliang Shen

“…Zinc ions have shown potential in modulating inflammation and cellular survival, making them a promising therapeutic approach for SCI. …”
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The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System by Sara Trabanelli, Federico La Manna, Marco Romano, Valentina Salvestrini, Michele Cavo, Marilena Ciciarello, Roberto M. Lemoli, Antonio Curti

“…In vitro differentiation of mesenchymal stromal cells (MSC) into osteocytes (human differentiated osteogenic cells, hDOC) before implantation has been proposed to optimize bone regeneration. …”
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Completely conserved VP2 residue K140 of KREMEN1-dependent enteroviruses is critical for virus-receptor interactions and viral infection by Zeyu Liu, Xue Li, Xiaohong Li, Xingyu Yan, Yuan Tian, Yue Zhao, Kexin Liu, Pei Hao, Shuye Zhang, Chao Zhang

“…ABSTRACT The KREMEN1 (KRM1) protein is a cellular receptor for multiple enteroviruses that cause hand, foot, and mouth disease (HFMD), including coxsackievirus CVA2, CVA3, CVA4, CVA5, CVA6, CVA10, and CVA12. …”
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Enhanced myofibroblast differentiation of eMSCs in intrauterine adhesions by Jun Song, Meiqi Li, Yuan Tao, Yumeng Li, Canrong Mai, Jingting Zhang, Lan Yao, Shaoquan Shi, Jianyong Xu

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Optimization of chemical transfection in airway epithelial cell lines by Tony J. F. Guo, Wan Yi Liang, Gurpreet K. Singhera, Jasmine Memar Vaghri, Janice M. Leung, Del R. Dorscheid

“…Consideration and optimization of cell line and transfection conditions may be useful for improving nonviral genetic techniques in vitro.…”
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Vitamin D Potentiates the Inhibitory Effect of MicroRNA-130a in Hepatitis C Virus Replication Independent of Type I Interferon Signaling Pathway by Xiaoqiong Duan, Yujuan Guan, Yujia Li, Shan Chen, Shilin Li, Limin Chen

“…Calcitriol, the bioactive metabolite of vitamin D, was reported to inhibit HCV production in a synergistic fashion with interferon, a treatment in vitro. Our previous study established that miR-130a inhibits HCV replication by restoring the host innate immune response. …”
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Unraveling the Role of the Tumor Extracellular Matrix to Inform Nanoparticle Design for Nanomedicine by Marco Cassani, Soraia Fernandes, Stefania Pagliari, Francesca Cavalieri, Frank Caruso, Giancarlo Forte

“…Abstract The extracellular matrix (ECM)—and its mechanobiology—regulates key cellular functions that drive tumor growth and development. …”
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Power Generation from Human Leukocytes/Lymphocytes in Mammalian Biofuel Cell by Güray Güven, Pablo Lozano-Sanchez, Arcan Güven

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Yinzhihuang injection induces apoptosis and suppresses tumor growth in acute myeloid leukemia cells. by Zhe Huang, Yunfu Shen, Xianming Fan, Qulian Guo, Wenzhe Ma

“…Consistent with the data in vitro, YZHI obviously suppressed subcutaneous xenograft growth in nude mice.…”
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Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice by Yuqing Sun, Jingfei Fu, Feiran Lin, Shengnan Li, Juan Du, Yi Liu, Yuxing Bai

“…Furthermore, the downstream cellular signaling pathway regulated by NO was also explored. …”
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Probiotics for the Control of Parasites: An Overview by Marie-Agnès Travers, Isabelle Florent, Linda Kohl, Philippe Grellier

“…In most cases, evidence for a beneficial effect was obtained by studies using animal models. In a few cases, cellular interactions between probiotics and pathogens or relevant host cells were also investigated using in vitro culture systems. …”
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RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori by Federico D’Agostino, Eva Pinatel, Alexandra Meynhardt, Vincenzo Scarlato, Andrea Vannini, Davide Roncarati

“…Summary: Ribonucleases are involved in several biological processes, including the turnover of structural and messenger RNAs and the specific processing of the cellular transcriptome. Here, we characterized the RNase Y from Helicobacter pylori. …”
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Dynamic localization of glucokinase and its regulatory protein in hypothalamic tanycytes. by Magdiel Salgado, Estefanía Tarifeño-Saldivia, Patricio Ordenes, Carola Millán, María José Yañez, Paula Llanos, Marcos Villagra, Roberto Elizondo-Vega, Fernando Martínez, Francisco Nualart, Elena Uribe, María de Los Angeles García-Robles

“…Thus, in tanycytes, GK can act as a molecular switch to arrest cellular responses to increased glucose.…”
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Contribution of In Vivo and Organotypic 3D Models to Understanding the Role of Macrophages and Neutrophils in the Pathogenesis of Psoriasis by Isabelle Lorthois, Daniel Asselineau, Nathalie Seyler, Roxane Pouliot

“…Current data from animal models and, to a lesser extent, three-dimensional in vitro models have confirmed the interest in leaning towards other immune cell types as a potential new cellular target for the treatment of the disease. …”
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Antiviral activity of an ACE2-Fc fusion protein against SARS-CoV-2 and its variants. by Ernesto Bermúdez-Abreut, Talia Fundora-Barrios, Diana Rosa Hernández Fernández, Enrique Noa Romero, Anitza Fraga-Quintero, Ana V Casadesús Pazos, Briandy Fernández-Marrero, Claudia A Plasencia Iglesias, Marilyn Clavel Pérez, Katya Sosa Aguiar, Belinda Sánchez-Ramírez, Tays Hernández

“…SARS-CoV-2 and its mutants use the angiotensin-converting enzyme 2 (ACE2) as a cellular entry receptor, which has triggered several therapeutic strategies against COVID-19 relying on the use of ACE2 recombinant proteins as decoy receptors. …”
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Patient-Specific Pluripotent Stem Cells in Neurological Diseases by Serpen Durnaoglu, Sermin Genc, Kursad Genc

“…Patient-specific iPSC-based modeling of neurogenetic and neurodegenerative diseases is an emerging efficient tool for in vitro modeling to understand disease and to screen for genes and drugs that modify the disease process. …”
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Mechanistic Insights into the Inhibition of Colorectal Cancer by BuShenFang through Adenomatous Polyposis Coli Expression and Wnt/β-catenin Pathway Regulation by Khang Wen Goh, Jie Ji, Hiu Ching Phang, Xian Gu, Rajesh Sreedharan Nair, Xiaohui Wei, Mohammed Tahir Ansari, Kai Bin Liew

“…The study utilized HCT116 and SW620 CRC cell lines, employing in vitro experiments including cell viability assays, colony formation, flow cytometry, and Western blot to examine the influence of BSF on cellular proliferation, apoptosis, migration, and the Wnt/β-catenin signaling pathway. …”
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Anti-PD-1 amplifies costimulation in melanoma-infiltrating Th1-like Foxp3+ regulatory T cells to alleviate local immunosuppression by Fernando Alvarez, Fan Huang, Sonia V Del Rincon, Mikhaël Attias, Tho-Alfakar Al-Aubodah, Roman Istomine, Paige McCallum, Abrahim Sleiman, Tamiko Nishimura, Yasser Riazalhosseini, Ciriaco A Piccirillo

“…In both HRs and LRs, Treg cells co-localize with CD8+ T cells in immunogenic regions of the tumor and display potent suppressive capacity in vitro. Further characterization revealed that melanoma-infiltrating Treg cells progressively acquire T-bet and interferon gamma expression, exclusively in HRs, and induction of this T helper cell 1 (Th1)-like phenotype in vitro led to CD8+ T cell evasion from Treg cell-mediated suppression. …”
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