Interleukin-1β Expression Is Required for Lysophosphatidic Acid-Induced Lymphangiogenesis in Human Umbilical Vein Endothelial Cells by Chih-Hsin Lin, JenHer Lu, Hsinyu Lee

“…Lysophosphatidic acid (LPA) is a lipid mediator which binds to G-protein-coupled receptors and regulates various cellular responses, including inflammation of endothelial cells. …”
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Development of a Reference Method and Materials for Quantitative Measurement of UV-Induced DNA Damage in Mammalian Cells: Comparison of Comet Assay and Cell Viability by Donald H. Atha, Alessandro Tona, Vytas Reipa

“…Maintaining the conditions of exposure within this range can improve DNA damage measurement fidelity, particularly if used as a quantitative reference method and to produce materials considered as an in vitro standard for the comet assay.…”
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YBX1 alleviates ferroptosis in osteoporosis via the ATF4/FSP1 axis in an m5C manner by Lei Tong, Yanbo Chen, Yan Gao, Xiaoming Gao, Yanming Hao

“…Methods We established an in vitro model of osteoporosis induced by D-galactose (D-gal) in MC3T3-E1 cells. …”
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Therapeutic potential and mechanisms of umbilical cord mesenchymal stem cells differentiating into tendon cells and promotion of rotator cuff tendon-bone healing by Youliang Shen, Yuelei Wang, Yidan Xu, Jie Wang, Chuqiang Yin, Zengshuai Han, Feng Shen, Ting Wang

“…Single-cell sequencing analysis revealed key cellular subpopulations and signaling pathways during differentiation. …”
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Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…The viral genome encodes soluble proteins imitating the structure of cellular cytokine receptors. These proteins compete with cellular receptors for cytokine binding, thus blocking the antiviral immune response. …”
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Phenolic Compounds and Anthocyanins in Legumes and Their Impact on Inflammation, Oxidative Stress, and Metabolism: Comprehensive Review by Rocio Guadalupe Hernández-Ruiz, Xochitl Citalli Olivares-Ochoa, Yahatziri Salinas-Varela, David Guajardo-Espinoza, Luis Gustavo Roldán-Flores, Edgar Alfonso Rivera-Leon, Andres López-Quintero

“…Legumes are a good source of phenolic compounds and anthocyanins that exert an antioxidant effect—they directly neutralize reactive oxygen species and free radicals, reducing oxidative stress. In vivo, in vitro, and clinical trial studies demonstrate that these compounds can modulate key cellular signaling pathways involved in inflammation and metabolism, improving insulin sensitivity and regulating lipid and glucose metabolism. …”
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Protein palmitoylation is involved in regulating mouse sperm motility via the signals of calcium, protein tyrosine phosphorylation and reactive oxygen species by Yuping Xiong, Chenchen Yi, Haixia Zheng, Ya Ni, Yamei Xue, Kun Li

“…Abstract Background Protein palmitoylation, a critical posttranslational modification, plays an indispensable role in various cellular processes, including the regulation of protein stability, mediation of membrane fusion, facilitation of intracellular protein trafficking, and participation in cellular signaling pathways. …”
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Therapeutic Potential of Herbal Medicines in Combating Particulate Matter (PM)-Induced Health Effects: Insights from Recent Studies by Aekkhaluck Intharuksa, Warunya Arunotayanun, Mingkwan Na Takuathung, Yaowatat Boongla, Siripat Chaichit, Suthiwat Khamnuan, Anchalee Prasansuklab

“…Numerous herbal medicines and phytochemicals have shown efficacy in mitigating PM-induced cellular damage through their ability to counteract oxidative stress, suppress pro-inflammatory responses, and enhance cellular defense mechanisms. …”
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Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines by Wei-Yi Lei, Shih-Chieh Hsiung, Shao-Hsuan Wen, Chin-Hsuan Hsieh, Chien-Lin Chen, Christopher Glenn Wallace, Chien-Chung Chang, Shuen-Kuei Liao

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Slowly Delivered Icariin/Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells to Promote the Healing of Calvarial Critical-Size Bone Defects by Tianlin Liu, Xin Zhang, Yuan Luo, Yuanliang Huang, Gang Wu

“…We hypothesized that the slowly released icariin could significantly promote the efficacy of SMC-PHBHHx/allogeneic BMSCs for repairing critical-size bone defects in rats. In in vitro cellular experiments, icariin at optimal concentration (10−6 mol/L) could significantly upregulate the osteogenesis- and angiogenesis-related genes and proteins, such as Runx2, ALP, osteocalcin, vascular endothelial growth factors, and fibroblast growth factors, as well as the mineralization of BMSCs. …”
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Characterization of subcutaneous and visceral de-differentiated fat cells by Yan Li, Houyu Zhang, Carlos F. Ibáñez, Meng Xie

“…Results: We show that in vitro-derived DFAT cells have lower adipogenic potential and distinct cellular composition compared to ASPCs. …”
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Radiolabeled Anti-Adenosine Triphosphate Synthase Monoclonal Antibody as a Theragnostic Agent Targeting Angiogenesis by Bok-Nam Park PhD, Su Jin Lee MD, PhD, Jung Hyun Roh MS, Kyung-Han Lee MD, PhD, Young-Sil An MD, PhD, Joon-Kee Yoon MD, PhD

“…Results: MKN-45 cells showed the highest in vitro cellular binding after 4 hours (0.00324 ± 0.00013%/μg), which was significantly inhibited by unlabeled ATPS mAb at concentrations of greater than 0.4 μM. …”
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Solid Self-Microemulsifying Drug Delivery System for Improved Oral Bioavailability of Relugolix: Preparation and Evaluation by Li ZL, Deng GX, Fang CZ, Zhao YQ, Yuan J, Chen L, Zhong HJ, Guo F

“…In vitro cellular uptake experiments revealed that the uptake of RLGL-S-SMEDDS by Caco-2 cells was three times higher than that of free RLGL, and that S-SMEDDS can enhance the drug absorption through lymphatic absorption and inhibition of intestinal transporter. …”
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RETRACTED ARTICLE: Bone mesenchymal stem cell-derived exosomal microRNA-7-5p inhibits progression of acute myeloid leukemia by targeting OSBPL11 by Duanfeng Jiang, Xin Wu, Xiaoying Sun, Wei Tan, Xin Dai, Youbang Xie, Ashuai Du, Qiangqiang Zhao

“…Overexpression of miR-7-5p curbed cellular proliferation and promoted apoptosis. Overexpression of OSBPL11 reversed the tumorigenic properties of miR-7-5p in AML cells in vitro. …”
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Human Liver Cells Expressing Albumin and Mesenchymal Characteristics Give Rise to Insulin-Producing Cells by Irit Meivar-Levy, Tamar Sapir, Dana Berneman, Tal Weissbach, Sylvie Polak-Charcon, Philippe Ravassard, Andreas G. Tzakis, Eytan Mor, Camillo Ricordi, Sarah Ferber

“…It is yet unknown whether hepatic-like stem cells or rather adult liver cells give rise to insulin-producing cells. Using an in vitro experimental system, we demonstrate that proliferating adherent human liver cells acquire mesenchymal-like characteristics and a considerable level of cellular plasticity. …”
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Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1 by Yong Yuan, Songqing Lai, Tie Hu, Fajia Hu, Chenchao Zou, Xiuqi Wang, Ming Fang, Jichun Liu, Huang Huang

“…Additionally, Pue significantly alleviated histopathological damage in MIRI-treated myocardium, as evidenced by HE staining and TUNEL assay. In vitro, Pue pretreatment significantly alleviated A/R-induced damage by decreasing LDH levels, increasing cellular activity, inhibiting autophagic lysosomal overactivation, inhibiting oxidative stress (ROS, LIP ROS, MDA), increasing antioxidant defense (SOD, GSH-Px), and increasing P62 protein expression while decreasing LC3II/I ratio. …”
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Rutaecarpine alleviates hepatic ischemia‒reperfusion injury in liver transplantation by inhibiting inflammatory response and oxidative stress by Yan Liu, Yan Liu, Feng Qi, Lun-Jian Xiang, Zhu-Jun Yi, Sheng-Wei Li

“…Mechanistically, bioinformatics analysis and in vivo and in vitro experiments revealed that PDE4B may be a key target by which Rut exerts its protective effect, and molecular docking and cellular thermal shift assay confirmed this result. …”
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Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining by Tatiane Oliveira, Camila A. Figueiredo, Carlos Brito, Alexander Stavroullakis, Anuradha Prakki, Eudes Da Silva Velozo, Getulio Nogueira-Filho

“…Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. …”
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Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing by Ursula Hopfner, Matthias M. Aitzetmueller, Philipp Neßbach, Michael S. Hu, Hans-Guenther Machens, Zeshaan N. Maan, Dominik Duscher

“…To investigate the in vitro effect of fibrin glue, cultivation experiments were performed and key cytokines for regeneration were quantified. …”
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Development and Evaluation of the Immunogenic Potential of an Unmodified Nucleoside mRNA Vaccine for Herpes Zoster by Shun Zhang, Xiaojie Wang, Tongyi Zhao, Chen Yang, Lulu Huang

“…Results: Codon-optimized mRNA sequences showed significantly higher protein expression levels in vitro compared to wild-type (WT) sequences. Vaccination with Vac07 demonstrated immunogenicity in mice that was comparable to, or even superior to, the licensed Shingrix vaccine, characterized by a stronger Th1-biased antibody response and a slightly more robust Th1-type cellular response. …”
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