The matricellular protein Fibulin-5 regulates β-cell proliferation in an autocrine/paracrine manner by Tomoko Okuyama, Takahiro Tsuno, Ryota Inoue, Setsuko Fukushima, Mayu Kyohara, Anzu Matsumura, Daisuke Miyashita, Kuniyuki Nishiyama, Yusuke Takano, Yu Togashi, Makiko Meguro-Horike, Shin-ichi Horike, Tatsuya Kin, A.M. James Shapiro, Hiromi Yanagisawa, Yasuo Terauchi, Jun Shirakawa

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Comparative analysis of lactaptin activity when produced in bacterial or eukaryotic expression systems by O. A. Koval, O. Y. Volkova, A. A. Gorchakov, S. V. Kulemzin, A. V. Tkachenko, A. A. Nushtaeva, E. V. Kuligina, V. A. Richter, A. V. Taranin

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Preclinical studies of the falnidamol as a highly potent and specific active ABCB1 transporter inhibitor by Baojie Liu, Yongzheng Lu, Ruihui Lin, Junbao Xu, Zilin Shang, Xinyu Hou, Xulong Shao, Zhifang Pan, Tao Yu, Weiguo Feng

“…The interaction between falnidamol and ABCB1 was evaluated by docking analysis and cellular thermal shift assay. Results Our data showed that falnidamol specifically reversed ABCB1-mediated MDR but not ABCG2-mediated MDR in vitro and in vivo. …”
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Mitochondrial DNA from endothelial cells activated the cGAS-STING pathway and regulated pyroptosis in lung ischaemia reperfusion injury after lung transplantation by Ying-nan Ju, Hu Li, Zi-peng Zhuo, Qing Yang, Wei Gao

“…Results: After 24 h of reperfusion, the expression of cGAS-STING-TBK1-IRF3 and pyroptotic proteins was significantly increased, and inhibitors of cGAS or STING ameliorated lung injury and reduced pyroptotic protein levels. In vitro, the inhibition of cGAS and STING reduced the activation of TBK and IRF3 and reduced cellular injury and death. …”
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Transient ocular hypertension remodels astrocytes through S100B. by Weiran Huang, Kenji Matsushita, Rumi Kawashima, Susumu Hara, Yuichi Yasukura, Kaito Yamaguchi, Shinichi Usui, Koichi Baba, Andrew J Quantock, Kohji Nishida

“…Astrocytes displayed weaker GFAP intensity (p < 0.0001) in the early-stage OHT mouse model, prior to the onset of hypertrophy, which was accompanied by an increase in GFAP mRNA expression (p < 0.0001) and a decrease in S100B mRNA expression (p < 0.001). In vitro-stretched astrocytes tended to contract and had fewer cellular processes and more elongated cell bodies. …”
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Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis by Sheng Wang, Zhaoyun Cheng, Xianjie Chen, Guoqing Lu, Xiliang Zhu, Gaojun Xu

“…In vitro, cell viability was detected by MTT assay, and cell apoptosis was assessed by caspase-3 level, Bax/Bcl-2 expression, and/or flow cytometry. …”
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Plants and fungi metabolites as novel autophagy inducers and senescence inhibitors by Rivka Ofir

“…Premature aging can be partially explained by inefficient autophagy (the process of cellular self-digestion that recycles intracellular components) and premature senescence (cease of cellular division without cell death activation). …”
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Bioactive Molecules for Skin Repair and Regeneration: Progress and Perspectives by Deyun Chen, Qian Hou, Lingzhi Zhong, Yali Zhao, Meirong Li, Xiaobing Fu

“…Bioactive molecules are practical tools for regulating cellular processes and have been applied to control cellular differentiation, dedifferentiation, and reprogramming. …”
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A Decade of FGF Receptor Research in Bladder Cancer: Past, Present, and Future Challenges by Erica di Martino, Darren C. Tomlinson, Margaret A. Knowles

“…Fibroblast growth factors (FGFs) orchestrate a variety of cellular functions by binding to their transmembrane tyrosine-kinase receptors (FGFRs) and activating downstream signalling pathways, including RAS/MAPK, PLCγ1, PI3K, and STATs. …”
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Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome by Bert R. Everaert, Steven J. Van Laere, Robrecht Lembrechts, Vicky Y. Hoymans, Jean-Pierre Timmermans, Christiaan J. Vrints

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Dynamic and electrophoretic light scattering measurements on microtubules at low concentrations. by Annitta George, Ernesto Alva, Lorenzo Brancaleon, Marcelo Marucho

“…The accurate characterization of microtubules is essential for understanding their roles in various biological activities in eukaryotic cellular processes. In vitro, experimental data on these systems often need more details and information on sample preparation protocols and experimental techniques. …”
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Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays by Yeon-Woo Cho, Min-Ji Kang, Joon-Ha Park, Yun-Sik Eom, Tae-Hyung Kim

“…The sustained release patterned array holds potential for constructing advanced therapeutic and disease state in vitro cellular models.…”
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Getah virus triggers ROS-mediated autophagy in mouse Leydig cells by Fengqin Li, Fengqin Li, Fengqin Li, Lishuang Deng, Tong Xu, Lei Xu, Zhiwen Xu, Zhiwen Xu, Siyuan Lai, Siyuan Lai, Yanru Ai, Yanru Ai, Yanqun Wang, Yanqun Wang, Guangwen Yan, Guangwen Yan, Ling Zhu, Ling Zhu

“…While previous studies have explored the epidemiology and pathogenicity of GETV in various species, its molecular mechanisms remain largely unexplored.MethodsThis study investigated the impact of GETV infection and associated molecular mechanisms on reactive oxygen species (ROS) and autophagy levels in mouse Leydig cells both in vivo and in vitro. The male mice and TM3 cells were treatment with N-acetylcysteine (NAC) to reduce cellular ROS levels. …”
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Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering by José Luis Hidalgo-Vicelis, Angélica Raquel Rivera-Contreras, Beatriz Hernández-Téllez, Gabriela Piñón-Zárate, Katia Jarquín-Yáñez, Tatiana Fiordelisio-Coll, José Manuel Saniger-Blesa, Gertrudis Hortensia González-Gómez, María Alicia Falcón-Neri, María Margarita Canales-Martínez, Andrés Eliú Castell-Rodríguez

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Multiplex basophil activation tests for allergy diagnosis: present and future applications by Ana Koren, Peter Korosec, Peter Korosec

“…The basophil activation test (BAT) has become a major cellular in vitro test for evaluating the allergenic activity of specific IgEs. …”
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Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis by Dana Ditgen, Emmanuela M. Anandarajah, Jan Hansmann, Dominic Winter, Guido Schramm, Klaus D. Erttmann, Eva Liebau, Norbert W. Brattig

“…The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. …”
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Role of astrocytes and microglia in hepatic encephalopathy associated with advanced chronic liver disease: lessons from animal studies by Wouter Claeys, Anja Geerts, Lien Van Hoecke, Christophe Van Steenkiste, Roosmarijn E. Vandenbroucke

“…Based on a large body of mostly in vitro evidence, ammonia-induced cellular and molecular disturbances include astrocyte swelling and oxidative stress. …”
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lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93 by Chuanchuan Shi, Yuqian Zhao, Qi Li, Jianguo Li

“…We also found that silencing SNHG14 also alleviated cellular injury processes of IL-1β and IL-6 in HK-2 cells via miR-93. …”
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Bioelectric stimulation outperforms brain derived neurotrophic factor in promoting neuronal maturation by María del Pilar Diego-Santiago, María Ujué González, Esther María Zamora Sánchez, Nuria Cortes-Carrillo, Carlos Dotti, Francesc Xavier Guix, Sahba Mobini

“…However, the broad effects of biochemical stimuli on multiple cellular functions limit their applicability in both in vitro models and clinical settings. …”
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Senescence-related circRNA circHIF-1α is associated with pancreatic cancer progression by Hao Hua, Yazu Deng, Dijie Zheng, Changhao Wu, Liwen Chen, Binbin Shi, Zhiwei He, Chao Yu

“…CircHIF-1α knockdown induces cellular senescence and suppresses PC proliferation in vitro experiments. …”
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