PPAR𝛾, PTEN, and the Fight against Cancer by Rosemary E. Teresi, Kristin A. Waite

“…Activation of PPAR𝛾 through agonists increases functional PTEN protein levels that subsequently induces apoptosis and inhibits cellular growth, which suggests that PPAR𝛾 may be a tumor suppressor. …”
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Proteins with RNA Chaperone Activity: A World of Diverse Proteins with a Common Task—Impediment of RNA Misfolding by Katharina Semrad

“…RNA chaperone activity is studied in vitro and in vivo using oligonucleotide- or ribozyme-based assays. …”
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Inhibitors of Complement Activity in Human Breast-Milk: A Proposed Hypothesis of Their Physiological Significance by Michael Oladipo Ogundele

“…Several natural components abundant in the fluid phase of human breast-milk have been shown to be inhibitors of complement activation in vitro, particularly the classical pathway. These include lysozyme, lactoferrin, lactalbumin alpha and other ligand chelators, complement regulator proteins and other specific soluble inhibitors of complement activation. …”
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A Cornflower Extract Containing N-Feruloylserotonin Reduces Inflammation in Human Skin by Neutralizing CCL17 and CCL22 and Inhibiting COX-2 and 5-LOX by Christophe Carola, Andrew Salazar, Christin Rakers, Franck Himbert, Quoc-Tuan Do, Philippe Bernard, Joerg von Hagen

“…Additionally, the same cornflower extract in another vehicle was evaluated in parallel with N-feruloylserotonin for cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) enzymatic cellular inhibition. The cornflower extract was shown to neutralize the two chemokines in vitro, inhibited COX-2 and 5-LOX, and demonstrated anti-inflammatory activities due mainly to the presence of N-feruloylserotonin. …”
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Newly identified single-nucleotide polymorphism associated with the transition from nonalcoholic fatty liver disease to liver fibrosis: results from a nested case-control study in... by Yitong Ling, Yu Xuan Yang, Yan Chun Chen, Jing Hao Wang, Dong Ge Feng, Shi Jian Xiang, Xiaoyu Zhang, Jun Lyu, Sha Sha Li

“…SAMM50-rs2073080 was upregulated in the NAFLD-associated fibrosis model. In vitro experiments on LX-2 cells showed that SAMM50-rs2073080 overexpression led to increased fibrosis, as indicated by higher cellular MDA levels and lower CAT and SOD levels, compared to the vector group.Conclusion Our research highlights a significant association of SAMM50-rs2073080 with the progression of NAFLD to hepatic fibrosis, and the in vitro experiments further corroborated these findings.…”
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Deciphering key nano-bio interface descriptors to predict nanoparticle-induced lung fibrosis by Jiayu Cao, Yuhui Yang, Xi Liu, Yang Huang, Qianqian Xie, Aliaksei Kadushkin, Mikhail Nedelko, Di Wu, Noel J. Aquilina, Xuehua Li, Xiaoming Cai, Ruibin Li

“…Results The nano-bio interactions induced diverse changes in the 4 characteristics of MeONPs and had variable effects on the 14 cellular functions, which were quantitatively evaluated in chemico and in vitro. …”
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Tasquinimod suppresses tumor cell growth and bone resorption by targeting immunosuppressive myeloid cells and inhibiting c-MYC expression in multiple myeloma by Marie Törngren, Rong Fan, Eline Menu, Helena Eriksson, Karin Vanderkerken, Andrew Chantry, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Philip Vlummens, Anke Maes, Catharina Muylaert, Elke De Bruyne, Holly Evans, Nathan De Beule, Dirk Hose, Ken Maes, Karine Breckpot, Kim De Veirman

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Electroporation of mouse embryonic stem cells with Neon device by I. P. Savchenkova, A. A. Savchenkova

“…Mouse embryonic stem cells are widely used as a promising material for producing of new cellular systems with desired properties in cellular and molecularbiology, pharmacology, virology, medicine, veterinary medicine and biotechnology. …”
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5-Methoxytryptophan Alleviates Dextran Sulfate Sodium-Induced Colitis by Inhibiting the Intestinal Epithelial Damage and Inflammatory Response by Yanling Wang, Jun Li, Qinyuan Yang, Zhenhang Zhu, Fang Cheng, Xiangyan Ai, Yang Liu, Dongbao Zhao, Futao Zhao, Peng Cheng

“…Finally, we examined the generation of cellular inflammation and analyzed the influence of 5-MTP on M1 polarization at the cellular level. …”
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In situ magnetic-field-assisted bioprinting process using magnetorheological bioink to obtain engineered muscle constructs by Hanjun Hwangbo, SooJung Chae, Dongryeol Ryu, GeunHyung Kim

“…A bioprinting process using ring magnets was developed for in situ bioprinting, resulting in well-aligned 3D cell structures and enhanced mechanotransduction effects on hASCs. In vitro analyses revealed upregulation of cellular activities, including myogenic-related gene expression, in hASCs. …”
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Utility of Lymphoblastoid Cell Lines for Induced Pluripotent Stem Cell Generation by Satish Kumar, Joanne E. Curran, David C. Glahn, John Blangero

“…A large number of EBV immortalized LCLs have been generated and maintained in genetic/epidemiological studies as a perpetual source of DNA and as a surrogate in vitro cell model. Recent successes in reprograming LCLs into iPSCs have paved the way for generating more relevant in vitro disease models using this existing bioresource. …”
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Human Brain Microvascular Endothelial Cells and Umbilical Vein Endothelial Cells Differentially Facilitate Leukocyte Recruitment and Utilize Chemokines for T Cell Migration by Shumei Man, Eroboghene E. Ubogu, Katherine A. Williams, Barbara Tucky, Melissa K. Callahan, Richard M. Ransohoff

“…In this study, we compared two different endothelial cellular models: transfected human brain microvascular endothelial cells (THBMECs) and human umbilical vein endothelial cells (HUVECs). …”
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A Novel Cortex Phellodendri Chinensis–Based Carbon Dots Platform for Remarkable Analgesia for Clinical Pain Management by Huimin Peng, Xingxing Zeng, Songbai Li, Xin Wang

“…Also, an MTT assay was performed to assess the in vitro cytotoxicity of CCDs against L929 cells. In vitro cytotoxicity studies revealed that L929 cells exhibited higher cell viability when treated with prepared CCDs. …”
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IMMUNOMODULATORY ACTIVITY OF THE LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS LB 86 ENZYME LYSATE by L. Lаzarenko, L. Orіabinska, T. Bohdan

“…Under the action of this enzyme lysate in vitro, the indicator of phagocytosis (PhI) of the peritoneal cavity macrophages of intact BALB/c mice was enhanced and after its intragastric administration to these mice, the increase of the parameters of spontaneous and stimulated NBT test of macrophages was observed and increase (on the 6th day after its introduction) in the levels of IL-12 and IFN-γ in the blood serum of mice treated with this enzyme lysate was noted against the background of an increase in the ratio of INF-γ/IL-4, which also indicated its potential ability to activate the cellular immune response. …”
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Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis by Lucía Arévalo-Híjar, Miguel Ángel Aguilar-Luis, Stefany Caballero-García, Néstor Gonzáles-Soto, Juana Del Valle-Mendoza

“…To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. …”
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Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG1 antibodies by Wen-Ting K. Tsai, Yinyin Li, Zhaojun Yin, Peter Tran, Qui Phung, Zhenru Zhou, Kun Peng, Dan Qin, Sien Tam, Christoph Spiess, Jochen Brumm, Manda Wong, Zhengmao Ye, Patrick Wu, Sivan Cohen, Paul J. Carter

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Fluorescence Lifetime Imaging of NAD(P)H in Patients’ Lymphocytes: Evaluation of Efficacy of Immunotherapy by Diana V. Yuzhakova, Daria A. Sachkova, Anna V. Izosimova, Konstantin S. Yashin, Gaukhar M. Yusubalieva, Vladimir P. Baklaushev, Artem M. Mozherov, Vladislav I. Shcheslavskiy, Marina V. Shirmanova

“…In this regard, the selection of an appropriate in vitro model and the choice of cellular response assay are critical for reliable predictions. …”
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Patient-derived xenografts from circulating cancer stem cells as a preclinical model for personalized pancreatic cancer research by Benedikt J. Wagner, Andreas Ettner-Sitter, Nicolas A. Ihlo, Merle Behr, Sebastian Koelbl, Stefan M. Brunner, Florian Weber, Bettina M. Rau, Hans J. Schlitt, Christoph Brochhausen, Rebecca Schoenmehl, Annalena Artinger, Dorothea Schott, Monika Pizon, Katharina Pachmann, Thiha Aung, Silke Haerteis, Christina Hackl

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Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering by Zahra Bashiri, Zahra Khosrowpour, Ali Moghaddaszadeh, Davod Jafari, Sanaz Alizadeh, Hajar Nasiri, Houman Parsaei, Zahra Keshtkaran, Meghdad Abdollahpour‐Alitappeh, Farshad Bargrizaneh, Behzad Rezaei, Sara Simorgh, Mazaher Gholipourmalekabadi

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Synthesis and Evaluation of Some New Isoquine Analogues for Antimalarial Activity by Chandra Nath Saha, Sanjib Bhattacharya, Dipak Chetia

“…The drug toxicity involves the formation of an electrophilic metabolite, amodiaquine quinoneimine (AQQI), which binds to cellular macromolecules leading to hepatotoxicity and agranulocytosis. …”
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