Development and Application of Quadruple Luminex xTAG Method for Simultaneous Detection of Japanese Encephalitis Virus, West Nile Virus, Yellow Fever Virus and Zika Virus by Junying SUN, Qiwen WU, Jichu LI, Chunhong LI, Pengju GUO, Yi HUANG, Feifei YIN

“…【Result】The Luminex xTAG method applied to detect JEV, WNV, YFV and ZIKV was established, and the optimal primer working concentration was 0.5, 0.5, 0.75, 0.5 µmol/L; the established hybridization system and reaction conditions were: 20 μL of magnetic bead working solution, 5 μL of PCR amplification product, and 75 μL of SAPE working buffer solution; the hybridization temperature, hybridization time and pH value were 37 ℃, 30 min, and 8.0, respectively. …”
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