Microcephaly protein ANKLE2 promotes Zika virus replication by Adam T. Fishburn, Cole J. Florio, Thomas N. Klaessens, Brian Prince, Neil A. B. Adia, Nicholas J. Lopez, Nitin Sai Beesabathuni, Sydney S. Becker, Liubov Cherkashchenko, Sophia T. Haggard Arcé, Vivian Hoang, Traci N. Shiu, R. Blake Richardson, Matthew J. Evans, Claudia Rückert, Priya S. Shah

“…We observe that ANKLE2 localization is drastically shifted to sites of NS4A accumulation during infection and that knockout of ANKLE2 reduces ZIKV replication in multiple human cell lines. This decrease in virus replication is coupled with a moderate increase in innate immune activation. …”
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Bispecific antibody-mediated redirection of NKG2D-CAR natural killer cells facilitates dual targeting and enhances antitumor activity by Congcong Zhang, Anja Waldmann, Winfried S Wels, Jasmin Röder, Anne Scherer, Malena Bodden, Jordi Pfeifer Serrahima, Anita Bhatti, Nina Müller, Pranav Oberoi

“…However, shedding or downregulation of NKG2D ligands (NKG2DL) can prevent NKG2D activation, resulting in escape of cancer cells from NKG2D-dependent immune surveillance.Methods To enable tumor-specific targeting of NKG2D-expressing effector cells independent of membrane-anchored NKG2DLs, we generated a homodimeric recombinant antibody which harbors an N-terminal single-chain fragment variable (scFv) antibody domain for binding to NKG2D, linked via a human IgG4 Fc region to a second C-terminal scFv antibody domain for recognition of the tumor-associated antigen ErbB2 (HER2). …”
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Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements by Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo

“…The anticoagulation assay showed that the four P2′ single-point mutants still had apparent inhibitory anticoagulation activity that selectively inhibited the human intrinsic coagulation pathway and had no influence on the extrinsic coagulation pathway or common coagulation pathway, which indicated that the single-point mutants had minimal effects on the anticoagulation activity of BF9-N17K. …”
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New pneumococcal serotype 20C is a WciG O-acetyltransferase deficient variant of canonical serotype 20B by Jigui Yu, Neil Ravenscroft, Peter Davey, Roshan Liyanage, Oliver Lorenz, Michelle M. Kuttel, Stephanie W. Lo, Feroze A. Ganaie, Moon H. Nahm

“…Consequently, genome-based serotyping/bioinformatic tools must scrutinize all cps genes for mutations that might inactivate/modify cps-encoded enzymes, ensuring effective tracking of emerging capsule variants in response to ongoing vaccination efforts.IMPORTANCEStreptococcus pneumoniae (pneumococcus) is a significant human pathogen known for producing a wide array of antigenically and structurally diverse capsule types, a fact that poses a serious challenge to the effectiveness of vaccines targeting pneumococcal capsule polysaccharide (PS). …”
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MathVision: An Accessible Intelligent Agent for Visually Impaired People to Understand Mathematical Equations by Muhammad Awais Ahmad, Tauqir Ahmed, Muhammad Aslam, Amjad Rehman, Faten S. Alamri, Saeed Ali Bahaj, Tanzila Saba

“…The LSTM architecture, chosen for its effectiveness with sequential data, is trained on a dataset containing paired examples of equations and corresponding human-generated descriptions. Fine-tuning includes optimizing hyperparameters for the task, and evaluation metrics such as the BLEU score are used to assess the model’s performance in generating accurate and contextually relevant textual representations for the detected mathematical content. …”
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Effects of 24 Weeks of Growth Hormone Treatment on Bone Microstructure and Volumetric Bone Density in Patients with Childhood-Onset Adult GH Deficiency by Hongbo Yang, Kemin Yan, Yuping Xu, Linjie Wang, Qi Zhang, Fengying Gong, Huijuan Zhu, Weibo Xia, Hui Pan

“…Effects of recombinant human growth hormone (rhGH) on bone microarchitecture in CO AGHD were insufficiently evaluated. …”
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MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy by Jiabin Liang, Jingxiu Zhao, Lin Yang, Qian Wang, Jing Liao, Jianhao Li, Weizhao Zhuang, Fanghong Li, Jinxian He, Yukuan Tang, Hanwei Chen, Chen Huang

“…Methods The in vitro studies included the effects of DSS duration on MSCs, and the characterization of DSS-Exo and Exo, followed by the assessment of RNA and protein expression levels of peritoneal fibrosis markers and inflammatory cytokines levels after treating human peritoneal mesothelial (HMrSV5) cells. In vivo experiments were conducted on a PF mouse model to observe cell morphology, collagen deposition, fibrosis localization, and to evaluate peritoneal functions such as filtration rate, urea nitrogen clearance, peritoneal thickness, and protein leakage. …”
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Immune Modulation Effect of Administration of Rice Bran Extract with Increased Solubility Fermented by <i>Lentinus edodes</i> UBC-V88 in Cyclophosphamide-Treated Mice Model by Gi-Jung Kim, Yelim Jang, Ji-Yoon Hong, Kyoung-Tae Kwon, Dae-Weon Kim, Dae-Jung Kang, Sung-Joo Hwang, Jung-Yun Lee, Emmanouil Apostolidis, Young-In Kwon

“…There is a global and increasing demand for natural ingredients that support the human immune response. In this study, the immunomodulatory effect and mechanism of action of rice bran extract fermented by <i>Lentinus edodes</i> was investigated. …”
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Metformin exerted tumoricidal effects on colon cancer tumoroids via the regulation of autophagy pathway by Roya Shabkhizan, Çığır Biray Avci, Sanya Haiaty, Marziyeh Sadat Moslehian, Fatemeh Sadeghsoltani, Ahad Bazmani, Mahdi Mahdipour, Leila Sabour Takanlou, Maryam Sabour Takanlou, Arezoo Rezaie Nezhad Zamani, Reza Rahbarghazi

“…Methods CRC tumoroids were developed using human umbilical vein endothelial cells (HUVECs), adenocarcinoma HT29 cells, and fibroblasts (HFFF2) in a ratio of 1: 2: 1 and 2.5% methylcellulose. …”
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Targeting NANOS1 in triple-negative breast cancer: synergistic effects of digoxin and PD-1 inhibitors in modulating the tumor immune microenvironment by Tangyi Wang, Yadian Lei, Jingwei Sun, Li Wang, Yuxin Lin, Zhijing Wu, Shoude Zhang, Chengzhu Cao, Chengzhu Cao, Chengzhu Cao, Haiyan Wang, Haiyan Wang, Haiyan Wang

“…Bioinformatics tools, including bc-GenExMiner v4.8, the Human Protein Atlas, and the TIMER database, were utilized for target identification. …”
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The two-component system ArlRS is essential for wall teichoic acid glycoswitching in Staphylococcus aureus by Marieke M. Kuijk, Emma Tusveld, Esther Lehmann, Rob van Dalen, Iñigo Lasa, Hanne Ingmer, Yvonne Pannekoek, Nina M. van Sorge

“…Since WTA represents a promising target for future immune-based treatments and vaccines, our findings provide important insight to align strategies targeting S. aureus WTA glycosylation patterns during infection.IMPORTANCEStaphylococcus aureus is a common colonizer but can also cause severe infections in humans. The development of antibiotic resistance complicates the treatment of S. aureus infections, increasing the need for antibiotic alternatives such as vaccines and therapies with bacterial viruses also known as phages. …”
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Lutetium-177 labeled iPD-L1 as a novel immunomodulator for cancer-targeted radiotherapy by Myrna Luna-Gutiérrez, Erika Azorín-Vega, Rigoberto Oros-Pantoja, Blanca Ocampo-García, Pedro Cruz-Nova, Nallely Jiménez-Mancilla, Gerardo Bravo-Villegas, Clara Santos-Cuevas, Laura Meléndez-Alafort, Guillermina Ferro-Flores

“…In vitro and in vivo studies demonstrated radiotracer stability in human serum (> 97% after 24 h evaluated by radio-HPLC), adequate affinity by the PDL1 protein (IC50 = 4.21 nM), and specific detection for PD-L1 assessed in 4T1, HCT116, and AR42J cancer cells, in which PD-L1 expression was verified by immunofluorescence and Western Blot assays. …”
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Probiotic Supplementation Alleviates Corticosterone-Induced Fatty Liver Disease by Regulating Hepatic Lipogenesis and Increasing Gut Microbiota Diversity in Broilers by Yuyan Feng, Wenqing Mei, Qu Chen, Xiaojing Chen, Yingdong Ni, Mingming Lei, Jie Liu

“…It has been suggested that gut microbiota modulation with probiotics ameliorates fatty liver disease in rodents and humans, yet it remains unclear whether the same results will also be obtained in poultry. …”
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In-vitro comparative thermo-chemical aging and penetration analyses of bioactive glass-based dental resin infiltrates by Syed Zubairuddin Ahmed, Abdul Samad Khan, Maram Alshehri, Fatimah Alsebaa, Fadak Almutawah, Moayad Mohammed Aljeshi, Asma Tufail Shah, Budi Aslinie Md Sabri, Sultan Akhtar, Mohamed Ibrahim Abu Hassan

“…Initial manual mixing for 1 h, followed by ultrasonic mixing for 3 min and then proceeded for the final mixing on a magnetic stirrer for 24 h in a dark room at ambient temperature. Human-extracted teeth were demineralized, and the experimental resins were infiltrated on the demineralized surface. …”
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MiR-4298 and lncKRTAP5-6-3 regulated Cathepsin D expression through ERK-MAPK signaling pathway in chronic UVB-damaged HaCaT cells by Xinling Chen, Feng Zhou, Yao Lin, Yue Xia, Jie Zhang, Wenyi Hou, Yu Sun, Wei Lai, Yue Zheng

“…In this study, we investigated the expression changes and interactions between miR4298 and lncKRTAP5-6-3 in chronically UVB-damaged human keratinocyte cell line (HaCaT) cells and explored miR4298-MAPK/ERK signaling pathway-Cathepsin D-lncKRTAP5-6-3 mechanisms in photoaging cells.MethodsHaCaT cells were irradiated with 12 mJ/cm2 UVB once a day for 7 days. miR-4298 mimics and miR-4298 inhibitors were transfected into HaCaT cells by lipo3000 transfection reagent, and the HaCaT cells were divided into three groups: blank control group; UVB-damaged group; and UVB damage+miR-4298 regulation (overexpression or inhibition) group. …”
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USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer by Fang Yuan, Juan Xu, Lingmei Xuan, Chan Deng, Wei Wang, Rong Yang

“…Results: We observed a significant upregulation of USP14 in human ESCA tissues and cell lines, especially in those with radioresistance. …”
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Research Progress and Prospect of Multi-robot Collaborative SLAM in Complex Agricultural Scenarios by MA Nan, CAO Shanshan, BAI Tao, KONG Fantao, SUN Wei

“…Thirdly, large language models will enhance human-robot interaction by enabling natural language commands, improving collaborative operations. …”
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ISU-ISU PRIORITAS DALAM PENERAPAN ECO-HOUSE BERDASARKAN GAYA HIDUP HEMAT ENERGI DAN RAMAH LINGKUNGAN by Yasintha Rahma Naura, Annisa Safira, Dewi Larasati

“…Land is also decreasing due to the increasing rate of human population growth and tends to be destructive to the surrounding environment regardless of its sustainability. …”
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Deteksi Dan Klasifikasi Hama Potato Beetle Pada Tanaman Kentang Menggunakan YOLOV8 by Daniel Geoffrey Manurung, Mohammad Ryan Pinasthika, Muhammad Azka Obila Vasya, Rania Aprilia Dwi Setya Putri, Agustinus Parasian Tampubolon, Rakan Fadhil Prayata, Septia Khoirin Nisa, Novanto Yudistira

“…Abstract   Potato (Solanum tuberosum L.) is a vital food crop with high economic value and a significant source of nutrition for humans. The production of potatoes is hindered by the infestation of the potato beetle (Leptinotarsa decemlineata Say), a pest that significantly impacts potato yields. …”
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Proximal, Microbiological and Nutritional Characterization in Chinese Potato Flour of the White Variety Colocasia Esculenta for Application in Functional Foods by F. López, L. Arboleda, V. González

“…Finally, the microbiological analysis of the product is determined, concluding that they are within the parameters established in wheat flours with the INEN 616: 2006 standard; thus it is considered of good quality, suitable for human consumption and consequently, for use in the food industry. …”
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