A broad spectrum Shigella vaccine based on VirG53 – 353 multiepitope region produced in a cell-free system by Girmay Desalegn, Charlotte Abrahamson, K. Ross Turbyfill, Lucy Pill-Pepe, Leslie Bautista, Chitradevi S. Tamilselvi, Dylan Dunn, Neeraj Kapoor, Barbara Sullinger, Matheo Herrera, Edwin V. Oaks, Jeff Fairman, Marcela F. Pasetti

“…The target antigen refinement and production improvement described here will facilitate the first-in-human studies.…”
Get full text

Design of a new class of broad-spectrum therapeutics targeted to drug-resistant bacteria by Amrutha Surendran, Agnes Turnbull, Allen Flockhart, Fern Findlay-Greene, Martyna Nowosielska, Donald Morrison, David J. Mincher, Samantha Donnellan

“…Key policy highlights Development of a novel class of antimicrobial compounds Drug screen against a WHO ‘high’ priority drug-resistant pathogen that poses the greatest threat to human health (methicillin-resistant Staphylococcus aureus) Drug screen against a surrogate for Mycobacterium tuberculosis, one of the leading causes of mortality worldwide.…”
Get full text

Heterogeneous focal adhesion cytoskeleton nanoarchitectures from microengineered interfacial curvature to oversee nuclear remodeling and mechanotransduction of mesenchymal stem cel... by Huayu Fan, Hui Zhao, Yan Hou, Danni Meng, Jizong Jiang, Eon-Bee Lee, Yinzheng Fu, Xiangdong Zhang, Rui Chen, Yongtao Wang

“…Methods Polydimethylsiloxane (PDMS)-based stencils were micropatterned to prepare the micropores for cell culture. The colonies of human mesenchymal stem cells (hMSCs) were formed by controlling cell seeding density to investigate the influences of cell density, curvature and heterogeneity on mechanotransduction. …”
Get full text

Deep proteomics and network pharmacology reveal sex- and age-shared neuropathic pain signatures in mouse dorsal root ganglia by Sabrina Grundtner, Julia R. Sondermann, Feng Xian, Daniel Malzl, Daniel Segelcke, Esther M. Pogatzki-Zahn, Jörg Menche, David Gómez-Varela, Manuela Schmidt

“…Among these, the vast majority (94 %) were also expressed and, in part, altered in human DRG of neuropathic pain patients, indicating evolutionary conservation. …”
Get full text

Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. by Sylvain Daton Kougblénou, Alidéhou Jerrold Agbankpé, Justin Gbèssohélé Béhanzin, Tamègnon Victorien Dougnon, Alidah Victonie Aniambossou, Lamine Baba-Moussa, Honoré Sourou Bankolé

“…Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. …”
Get full text

Encoding of cerebellar dentate neuron activity during visual attention in rhesus macaques by Nico A Flierman, Sue Ann Koay, Willem S van Hoogstraten, Tom JH Ruigrok, Pieter Roelfsema, Aleksandra Badura, Chris I De Zeeuw

“…To study cerebellar activity during visual attention we recorded extracellular activity of dentate nucleus (DN) neurons in two non-human primates (NHPs). NHPs were trained to read the direction indicated by a peripheral visual stimulus while maintaining fixation at the center, and report the direction of the cue by performing a saccadic eye movement into the same direction following a delay. …”
Get full text

Level of fecal neopterin and calprotectin in asymptomatic and symptomatic norovirus-infected children with malnutrition in Indonesia by Anisa Lailatul Fitria, Zayyin Dinana, Aussie Tahta Maharani, Alinda Syifa Fumizuki, Faiz Nabila Mumtazya, Dominikus Raditya Atmaka, Laura Navika Yamani, Juniastuti, Maria Inge Lusida, Soetjipto, Septa Indra Puspikawati

“…We assessed the fecal neopterin and calprotectin from the stools samples collected during viral diarrhea surveillance in Surabaya, East Java, Indonesia, using commercially ELISA kit from BT Lab (Zhejiang, China) for human neopterin and calprotectin. The statistical differences were tested using Kruskal-Wallis nonparametric test. …”
Get full text

An assessment of breast cancer HER2, ER, and PR expressions based on mammography using deep learning with convolutional neural networks by Shun Zeng, Hongyu Chen, Rui Jing, Wenzhuo Yang, Ligong He, Tianle Zou, Peng Liu, Bo Liang, Dan Shi, Wenhao Wu, Qiusheng Lin, Zhenyu Ma, Jinhui Zha, Yonghao Zhong, Xianbin Zhang, Guangrui Shao, Peng Gong

“…Abstract Mammography is the recommended imaging modality for breast cancer screening. Expressions of human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER), and progesterone receptor (PR) are critical to the development of therapeutic strategies for breast cancer. …”
Get full text

Damage symptoms, development, and reproductive performance of the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on fodder maize and cob by Hend O. Mohamed, Hassan F. Dahi, Azza A. Awad, Walaa E. Gamil, Bassam F. Fahmy

“…The results exhibited that the FAW larvae were able to feed on and infest both fodder maize and corn cobs, causing significant damage particularly to the cobs making them unsuitable for marketing and human consumption. Moreover, the larvae and pupae from the corn cobs had the greatest weight and were significantly affected by food items. …”
Get full text

Phase 1 study of safety, tolerability, and efficacy of intradermal DNA vaccine ASP2390 in adults allergic to house dust mites by Thomas Kayser, MD, BS, Ronald Smulders, MD, PhD, Tomohiro Kusawake, PhD, Erik Wambre, PhD, Gurunadh R. Chichili, PhD, Mary B. Blauwet, DrPH, Anna Spence, MS, Melanie Patton, BS, Rima Tabash, PhD, Hannah A. DeBerg, PhD, Sugandhika Khosa, MS, Philipp Badorrek, MD, Jens M. Hohlfeld, MD, Brian C. Ferslew, PharmD, PhD

“…Objective: We evaluated the safety, tolerability, and efficacy of first-in-human intradermal ASP2390 in adults with HDM allergy. …”
Get full text

Electricity Theft Detection Using Machine Learning in Traditional Meter Postpaid Residential Customers: A Case Study on State Electricity Company (PLN) Indonesia by Alief Pascal Taruna, Galih Arisona, Dwi Irwanto, Arif Bijak Bestari, Wildan Juniawan

“…These findings demonstrate the model’s effectiveness in delivering reliable TO recommendations, supporting PLN’s operational strategies, and offering practical benefits through a more objective, standardized TO process that minimizes human error and improves efficiency.…”
Get full text

Traditional cereal processing technologies and their importance to African communities: A review by Chakare Benhura, Nyasha G Mushonga, Amanda Muguse, Kudzai Kamunhukamwe, Clareta Mpezani, Lisa Chibira, Mellisa Taruvinga, Santa M Patel

“…Cereal technology is the processing of cereal grain into starch and other nutrients for consumption by humans or animals. The technology involves all processes that the grain is taken through from the farm till it reaches the table for consumption. …”
Get full text

Assessing Knowledge, Attitudes, and Practices of Healthcare Workers regarding Biomedical Waste Management at Biyem-Assi District Hospital, Yaounde: A Cross-Sectional Analytical Stu... by Sylvain Honoré Woromogo, Gwladys Guetsé Djeukang, Félicité Emma Yagata Moussa, Jesse Saint Saba Antaon, Kingsley Ngah Kort, Pierre Marie Tebeu

“…Healthcare workers are responsible for the proper management of this waste for human safety and for the protection of the environment. …”
Get full text

ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway by Xi Zhu, Xiaogang Zhang, Xinpeng Cong, Luoning Zhu, Zhongping Ning

“…Mice were infused with Ang II (2000 ng/kg/min) and were administrated with recombinant human ANGPTL4 (rhANGPTL4, 20 μg/kg/day) for 3 weeks. …”
Get full text

Chronic NaAsO2 exposure promotes migration and invasion of prostate cancer cells by Akt/GSK-3β/β-catenin/TCF4 axis-mediated epithelial-mesenchymal transition by Zhi-Hui Zhang, Hai-Xin Yan, Ming-Dong Liu, Feng-Wen Niu, Kai Yao, Shi-Yao Feng, Xi Li, Yuan-Hua Chen, Dong-Dong Xie

“…Inorganic arsenic is a Class I human Carcinogen. However, the role of chronic inorganic arsenic exposure on prostate cancer metastasis still unclear. …”
Get full text

Mechanistic insights into 125I seed implantation therapy for Cholangiocarcinoma: focus on ROS-Mediated apoptosis and the role of GPX2 by Jun Luo, Zheng Yao, Weiren Liang, Danjun Song, Hui Zeng, Yi Jiang, Zhehan Bao, Jiaping Zheng, Yinan Ding

“…Materials and methods Human cholangiocarcinoma cell lines QBC939 and RBE were purchased for in vitro studies. …”
Get full text

Rh and Kell phenotyping in voluntary blood donors: A study from a tertiary care blood center of western India by Sangita D. Shah, Nidhi M. Bhatnagar, Mamta C. Shah, Garima H. Thakkar, Ujjwal Ahuja, Anu Patel, Drashti Gajera, Priya G. Kalavadiya

“…INTRODUCTION: In human beings, there are 45 blood group systems and 360 antigens currently recognized by ISBT (July 2023). …”
Get full text

Signal Processing-Free Intelligent Model for Power Quality Disturbances Identification by Mohammed F. Al-Mashdali, Asif Islam, Abdulbasit Hassan, Md Shafiullah, Mujahed Al-Dhaifallah, Khalid Al Fuwail

“…By directly examining PQD images generated from signal data, these models reduce the requirement for human-crafted features. The study analyzes alternative CNN setups, training datasets, and disturbance types to measure model performance. …”
Get full text

RAB3GAP2 dysregulation in adult T-cell leukemia/lymphoma (ATLL) compared to acute lymphoblastic leukemia (ALL): a molecular perspective by Samira Pourrezaei, Arash Letafati, Ghazale Molaverdi, Mehdi Norouzi, Sayed-Hamidreza Mozhgani

“…Abstract Adult T-cell leukemia/lymphoma (ATLL) is a type of blood cancer related to human T-cell lymphotropic virus type 1 (HTLV-1). …”
Get full text

Comparative genetic analysis of blood and semen samples in sperm donors from Hunan, China by Chuan Huang, Li-Ming Chu, Bo Liang, Hui-Lan Wu, Bai-Shun Li, Shuai Ren, Mei-Ling Hou, Hong-Chuan Nie, Ling-Yin Kong, Li-Qing Fan, Juan Du, Wen-Bing Zhu

“…This study aimed to determine whether pathogenic variants identified in the sperm of young semen donors are also present in their blood, and whether matching results for blood are consistent with results for sperm.Methods We included 40 paired sperm and blood samples from 40 qualified semen donors at the Hunan Province Human Sperm Bank of China. All samples underwent exome sequencing (ES) analysis, and the pathogenicity was assessed according to the American College of Medical Genetics (ACMG) guidelines. …”
Get full text