Two Cysteines in Raf Kinase Inhibitor Protein Make Differential Contributions to Structural Dynamics In Vitro by Hyun Sang Cho, Mohammad Faysal Al Mazid, Eun-Young Lee, Md Abu Rayhan, Hyoun Sook Kim, Byung Il Lee, Hye Jin You

“…Here, we identified the role of cysteine 133 in RKIP structural dynamics using recombinant human RKIP (rhRKIP) proteins purified from <i>Escherichia coli</i> BL21(DE3) cells. …”
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miR-155 Predicts Long-Term Mortality in Critically Ill Patients Younger than 65 Years by Frank Tacke, Martina E. Spehlmann, Mihael Vucur, Fabian Benz, Mark Luedde, David Vargas Cardenas, Sanchari Roy, Sven Loosen, Hans-Joerg Hippe, Norbert Frey, Christian Trautwein, Alexander Koch, Christoph Roderburg, Tom Luedde

“…Our study is the first to describe an age-dependent miRNA-based prognostic biomarker in human diseases.…”
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Identification and optimization of urban wetland ecological networks in highly urbanized areas: A case study of Haidian District, Beijing by Wang Zecheng, Zhao Xinsheng, Cui Lijuan, Lei Yinru, Guo Ziliang, Wang Jinzhi, Li Jing, Zhai Xiajie, Wang Rumiao, Li Wei

“…With the rapid development of urbanization, urban wetlands have been severely damaged by human activities and natural factors, leading to problems such as reduced area and decreased connectivity between wetlands. …”
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Protocol for a qualitative study exploring haemodialysis dependent patients’ arteriovenous fistula experience, values and concerns in Sydney, Australia by Vasi Naganathan, Sarah Joy Aitken, Bethany Miriam Stavert, Susan Monaro, Lisa Tienstra

“…Findings from this study will give a nuanced insight into the experiences of patients on haemodialysis with respect to their AVF.Ethics and dissemination Ethical approval for this study was provided by the Sydney Local Health District Human Research Ethics Committee (REGIS identifier: 2021/ETH00362, CH reference number: CH62/6/2021-033). …”
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Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State by Marie Koren-Gluzer, Mira Rosenblat, Tony Hayek

“…The direct effect of PON2 was shown by transfecting human PON2 (hPON2) into PON2KO MPM. PON2 transfection attenuated the macrophages’ response to M1 activation and enhanced M2 response. …”
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Suppressive Activity of a Macrolide Antibiotic, Roxithromycin, on Pro-Inflammatory Cytokine Production in Vitro and in Vivo by H. Suzaki, K. Asano, S. Ohki, K. Kanai, T. Mizutani, T. Hisamitsu

“…In the first experiments, we examined the effect of RXM on in vitro cytokine production from lipopolysaccharide (LPS)-stimulated human peripheral blood monocytes. The monocytes were cultured in the presence of various doses of the agent. …”
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Abortion Laws in Indonesia and Islam: A Clash of Morality, Religion, and Rights by Salsabila Triandani, Windiahsari Windiahsari, Ali Masyhar, Aprila Niravita

“…The paper also addresses ongoing debates over potential legal reforms, considering global human rights perspectives and the influence of Islamic jurisprudence in shaping reproductive health policies. …”
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Uncertainties of the rainfall regime in a tropical semi-arid region: the case of the State of Ceará by Eunice Maia de Andrade, Maria Gina Torres Sena, Antonio Givanilson Rodrigues da Silva, Francisco Jairo Soares Pereira, Fernando Bezerra Lopes

“…The rural population of a tropical semi-arid region such as the northeast of Brazil is directly dependent on rainfall for agricultural production, pasture for livestock, and the storage of drinking water for human consumption. The region is characterised by the frequent occurrence of dry years and consecutive dry days (CDD) during the rainy season, demonstrating the vulnerability of rainfed agriculture. …”
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A multistage drug delivery approach for colorectal primary tumors and lymph node metastases by Yihang Yuan, Quanjun Lin, Hai-Yi Feng, Yunpeng Zhang, Xing Lai, Mao-Hua Zhu, Jue Wang, Jiangpei Shi, Yanhu Huang, Lele Zhang, Qin Lu, Zeli Yuan, Jonathan F. Lovell, Hong-Zhuan Chen, Peng Sun, Chao Fang

“…The liposomal membrane contains 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), two phospholipids sensitive to secreted phospholipase A2 in human colorectal tumors. In an orthotopic colorectal murine tumor model, phospholipase-induced membrane permeabilization triggers the liberation of DMSN from liposomes for enhanced tumor penetration, conferring an enhanced suppression for the primary tumor. …”
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Mutation-Specific Phenotypes in hiPSC-Derived Cardiomyocytes Carrying Either Myosin-Binding Protein C Or α-Tropomyosin Mutation for Hypertrophic Cardiomyopathy by Marisa Ojala, Chandra Prajapati, Risto-Pekka Pölönen, Kristiina Rajala, Mari Pekkanen-Mattila, Jyrki Rasku, Kim Larsson, Katriina Aalto-Setälä

“…We studied the properties of HCM cardiomyocytes (CMs) derived from patient-specific human induced pluripotent stem cells (hiPSCs) carrying either MYBPC3-Gln1061X or TPM1-Asp175Asn mutation. …”
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Decoding the general role of tRNA queuosine modification in eukaryotes by Jorge Díaz-Rullo, Luis González-Moreno, Araceli Del Arco, José Eduardo González-Pastor

“…These bioinformatics results provide evidence to support for the first time in eukaryotes that the wide diversity of phenotypes associated with tRNA Q-modification previously described in various species would directly depend on the control of Q-genes translation, and would allow prediction of unknown Q-dependent processes, such as Akt activation and p53 expression, which we have tested in human cancer cells. Considering the relevance of the Q-related processes, our findings may support further exploration of the role of Q in cancer and other pathologies. …”
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Discovery of Novel Nav1.7-Selective Inhibitors with the 1H-Indole-3-Propionamide Scaffold for Effective Pain Relief by Gaoang Wang, Hang Wu, Yingying Wang, Xiangying Liu, Shuijiao Peng, Wenxing Wang, Meijing Wu, Yifei Liu, Ercheng Wang, Zhe Wang, Lei Xu, Xiaojian Wang, Wei Yang, Haiyi Chen, Xi Zhou, Tingjun Hou

“…Nav1.7 is considered a promising target for developing next-generation analgesic drugs, given its critical role in human pain pathologies. Although most reported inhibitors with strong in vitro activity and high selectivity share the aryl sulfonamide scaffold, they failed to demonstrate marked clinical efficacy. …”
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Haptoglobin Genotype-Dependent Anti-Inflammatory Signaling in CD163+ Macrophages by R. Clive Landis, Pandelis Philippidis, Jan Domin, Joseph J. Boyle, Dorian O. Haskard

“…In this study, we examined whether scavenging of polymorphic Hp:Hb complexes differentially generated downstream anti-inflammatory signals in cultured human macrophages culminating in interleukin (IL)-10 secretion. …”
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Immunization‐related complex regional pain syndrome: A systematic review of case reports by William K. Copenhaver, Brandon J. Goodwin, Alexa Simonetti, Kunal P. Shah, Nicholas J. Averell, David F. Lo, Richard T. Jermyn

“…Studies included cases of CRPS development following tetanus, hepatitis B, hepatitis A, rubella, influenza, tetanus–diphtheria, human papillomavirus, and COVID‐19 vaccine administration. …”
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Evaluation of hydrogel formulated with seaweed extract (Ulva lactuca) for incised wound healing in white rats (Rattus norvegicus) by Stephanie Taniwan, Refi Ikhtiari, Stephanie Taniwan, Fiska Maya Wardhani

“…Skin, as the largest organ in the human and animal body, serves as the main protection against various external factors such as sharp objects, extreme temperatures, chemicals, or physical trauma. …”
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Fueling the Innovation Spark: How Employee Oriented HR Practices and Career Satisfaction Fosters Innovative Work Behavior? by Syed Mudasser Abbas, Zhiqiang Liu, Arshad Mahmood, Asma Mushtaq

“…This study aims to investigate how employee-oriented human resource (HR) practices (salary, job enrichment, job stability, training) can influence career satisfaction that subsequently affects employees' innovative behavior. …”
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IUPHAR Review: Alpha6-containing GABAA receptors – Novel targets for the treatment of schizophrenia by Lih-Chu Chiou, Werner Sieghart

“…So far, the beneficial actions of these modulators have been demonstrated in animal models of neuropsychiatric disorders, only. Future human studies have to investigate the safety and possible side effects of these modulators and to clarify, to which extent individual symptoms of schizophrenia can be reduced by these drugs in patients during acute and chronic dosing.…”
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On the Design and Development of Planar Monopole Antenna for Bone Crack/Void Detection by Ananda Venkatesan Boologam, Kalimuthu Krishnan, Sandeep Kumar Palaniswamy, Sachin Kumar, Shreya Bhowmik, Nivesh Sharma, Deepesh Vaish, Sourish Chatterjee

“…This method can detect bone fractures in a noninvasive manner. The human arm model is constructed, and the effect of bone cracks of different lengths on the resonating frequency is investigated. …”
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The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells by Alberto Azzalin, Elena Sbalchiero, Giulia Barbieri, Silvia Palumbo, Cristina Muzzini, Sergio Comincini

“…Doppel (Dpl) protein is the paralogue of the cellular prion (PrPC) protein. In humans, Dpl is expressed almost exclusively in testis where it is involved in spermatogenesis. …”
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Stabilizing milk-derived extracellular vesicles (mEVs) through lyophilization: a novel trehalose and tryptophan formulation for maintaining structure and Bioactivity during long-te... by Alan B. Dogan, Spencer R. Marsh, Rachel J. Tschetter, Claire E. Beard, Md R. Amin, L. Jane Jourdan, Robert G. Gourdie

“…In addition to undertaking established mEV assays of structure and function on our preparations, we introduce a novel, efficient, high throughput assay of mEV bioactivity based on Electric Cell Substrate Impedance Sensing (ECIS) in Human dermal fibroblast monolayers. By adding appropriate excipients, such as trehalose and tryptophan, we describe a protective formulation that preserves mEV bioactivity during long-term, room temperature storage. …”
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