A guide to selecting high-performing antibodies for Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PPP2R5D) for use in Western Blot, immunoprecipi... by Carl Laflamme, Kathleen Southern, Riham Ayoubi, Maryam Fotouhi, Charles Alende

“…These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. …”
Get full text

Development of digital competence of university teachers and teaching staff by Aksar A. Eltemerov

“…This global digitalization of the human living environment changes the very paradigm of thinking of the new generation. …”
Get full text

Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures by Jarline Encarnación-Medina, Rosa I. Rodríguez-Cotto, Joseph Bloom-Oquendo, Mario G. Ortiz-Martínez, Jorge Duconge, Braulio Jiménez-Vélez

“…This study assesses ABCC1–4 gene expression changes and proinflammatory cytokine (IL-6, IL-8) release in human bronchial epithelial cells (BEAS-2B) exposed to PM2.5 organic extract, budesonide (BUD, used to control inflammation in asthmatic patients), and a cotreatment (Co-T: PM2.5 and BUD). …”
Get full text

Malian field isolates provide insight into Plasmodium malariae intra-erythrocytic development and invasion. by Francois Dao, Amadou Niangaly, Fanta Sogore, Mamadou Wague, Djeneba Dabitao, Siaka Goita, Aboubacrin S Hadara, Ousmaila Diakite, Mohamed Maiga, Fatoumata O Maiga, Chantal Cazevieille, Cecile Cassan, Arthur M Talman, Abdoulaye A Djimde, Alejandro Marin-Menendez, Laurent Dembélé

“…Plasmodium malariae is the third most prevalent human malaria parasite species and contributes significantly to morbidity. …”
Get full text

Effect of Pentoxifylline on Microalbuminuria in Diabetic Patients: A Randomized Controlled Trial by Shahrzad Shahidi, Marziyeh Hoseinbalam, Bijan Iraj, Mojtaba Akbari

“…Pentoxifylline is a nonspecific phosphodiesterase inhibitor with anti-inflammatory properties. Human studies have proved its antiproteinuric effect in patients with glomerular diseases, but this study was designed to assess the effects of add-on pentoxifylline to available treatment on reduction of microalbuminuria in diabetic patients without glomerular diseases. …”
Get full text

Epigenetics and Inflammatory Markers: A Systematic Review of the Current Evidence by Valentina Gonzalez-Jaramillo, Eliana Portilla-Fernandez, Marija Glisic, Trudy Voortman, Mohsen Ghanbari, Wichor Bramer, Rajiv Chowdhury, Tamar Nijsten, Abbas Dehghan, Oscar H. Franco, Jana Nano

“…We included studies conducted on humans that examined the association between epigenetic marks (DNA methylation and/or histone modifications) and a comprehensive list of inflammatory markers. …”
Get full text

Effect of Tumor Necrosis Factor Family Member LIGHT (TNFSF14) on the Activation of Basophils and Eosinophils Interacting with Bronchial Epithelial Cells by Huai Na Qiu, Chun Kwok Wong, Jie Dong, Christopher Wai-Kei Lam, Zhe Cai

“…In this study, we investigated the effects of LIGHT on the coculture of human basophils/eosinophils and bronchial epithelial BEAS-2B cells. …”
Get full text

Formaldehyde Exposure and Its Potential Health Risk in Some Beauty Salons in Kumasi Metropolis by Noah Kyame Asare-Donkor, James Kusi Appiah, Vincent Torve, Ray Bright Voegborlo, Anthony Apeke Adimado

“…In order to assess the exposure of cosmetologists to formaldehyde, the indoor air in sixty beauty salons across the ten submetros in Kumasi were sampled to determine the formaldehyde levels and the associated noncarcinogenic human health risks. Sampling was done using System Service Innovation Incorporation air sampler model 1000i, and the MBTH spectrophotometric method was used for analysis. …”
Get full text

Exploring anti-cancer activities of epidermal growth factor-immobilized polymeric nanoparticles by Shota Yamamoto, Chia-Jung Chang, Masao Kamimura, Jun Nakanishi

“…Initial mechanistic studies on phosphorylation signaling and cholesterol depletion revealed that EGF-polystyrene nanoparticles exhibited cytotoxicity against human cervical adenocarcinoma HeLa cells via local enhancement of EGFR activity in membrane rafts. …”
Get full text

Sirtuin-2 Protects Neural Cells from Oxidative Stress and Is Elevated in Neurodegeneration by Preeti Singh, Peter S. Hanson, Christopher M. Morris

“…SH-SY5Y viability in response to oxidative stress induced by diquat or rotenone was measured following SIRT2 overexpression or inhibition of deacetylase activity, along with α-synuclein aggregation. SIRT2 in human tissues was evaluated using Western blotting, immunohistochemistry, and fluorometric activity assays. …”
Get full text

Employing the concept of stacking ensemble learning to generate deep dream images using multiple CNN variants by Lafta Alkhazraji, Ayad R. Abbas, Abeer S. Jamil, Zahraa Saddi Kadhim, Wissam Alkhazraji, Sabah Abdulazeez Jebur, Bassam Noori Shaker, Mohammed Abdallazez Mohammed, Mohanad A. Mohammed, Basim Mohammed Al-Araji, Abdulkareem Z. Mohmmed, Wasiq Khan, Bilal Khan, Abir Jaafar Hussain

“…Such approaches can also be useful for promoting interest in human artwork, particularly surrealist images. Accordingly, in the present, a stacking ensemble Deep Dream model was developed that aids psychiatrists and psychologists in addressing the challenge of mimicking hallucinations. …”
Get full text

Characterization of Broad Spectrum Bacteriophage vB ESM-pEJ01 and Its Antimicrobial Efficacy Against Shiga Toxin-Producing <i>Escherichia coli</i> in Green Juice by Eun Jeong Park, Seungki Lee, Jong Beom Na, Ye Bin Kim, Kee Man Lee, Seon Young Park, Ji Hyung Kim

“…Shiga toxin-producing <i>Escherichia coli</i> (STEC) infections have increased in humans, animals, and the food industry, with ready-to-eat (RTE) food products being particularly susceptible to contamination. …”
Get full text

Effect of plasma assisted combustion on emissions of a premixed nitrous oxide fuel blend by Boj N. Villanueva, Po-Hung Lin, Yueh-Heng Li, Jaime P. Honra

“…However, research on the fuel blend is relatively scant, especially regarding its emission characteristics, as the composition may produce high NOx emissions, which are harmful to both human society and the environment. This study sought to utilize Nanosecond Pulsed Dielectric Barrier Discharge plasma as an emission control mechanism as not only has it found success as a pollution control mechanism, but also as a combustion enhancement one. …”
Get full text

Exploring BCL2 regulation and upstream signaling transduction in venetoclax resistance in multiple myeloma: potential avenues for therapeutic intervention by Rodrigo Fonseca, Yuan Xiao Zhu, Laura A. Bruins, Joseph Ahmann, Cecilia de Bonolo Campos, Esteban Braggio, Xianfeng Chen, Mariano Arribas, Susie Darvish, Seth Welsh, Erin Meermeier, Kiran K. Mangalaparthi, Richard K. Kandasamy, Greg Ahmann, J. Erin Wiedmeier-Nutor, Akhilesh Pandey, Marta Chesi, P. Leif Bergsagel, Rafael Fonseca

“…We conducted a multi-omic characterization of established VTX-resistant isogenic human myeloma cell lines (HMCL) and primary MM patient samples pre- and post-VTX treatment. …”
Get full text

Toward <i>Mycobacterium tuberculosis</i> Virulence Inhibition: Beyond Cell Wall by Maria S. Kotliarova, Mikhail S. Shumkov, Anna V. Goncharenko

“…<i>Mycobacterium tuberculosis</i> (Mtb) is one of the most successful bacterial pathogens in human history. Even in the antibiotic era, Mtb is widespread and causes millions of new cases of tuberculosis each year. …”
Get full text

Spatial frequency preferences of representations of indoor and natural scene categories in scene-selective regions under different conditions of contrast by Yuanyuan Zhang, Qiaomu Miao, Baolin Liu

“…The results showed the scene-selective regions were more efficient in distinguishing natural scene categories in LSF, and the LSF was preferentially used along with high luminance contrast in recognition of natural scenes. On the other hand, humans preferentially used HSF information in distinguishing indoor scenes.DiscussionThis distinct SF preferences maybe caused by the different aspects of information conveyed by LSF and HSF, as well as the different strategies of spatial perception in natural and indoor scenes recognition.…”
Get full text

Development of a Semiconductor Sequencing-based Panel for Screening Individuals with Lynch Syndrome by Ryia-Illani Mohd Yunos, Nurul-Syakima Ab Mutalib, Janice Khor Sheau Sean, Sazuita Saidin, Mohd Ridhwan Abd Razak, Isa Md. Rose, Ismail Sagap, Luqman Mazlan, Rahman Jamal

“…The sensitivity and specificity of the identified mutations were determined by sequencing the serially diluted DNA from two human cancer cell lines, HCT 116 and LN-18. An average of 92 % of reads were mapped to the target region with 98 % uniformity. …”
Get full text

Status Epilepticus due to Intraperitoneal Injection of Vehicle Containing Propylene Glycol in Sprague Dawley Rats by Evon S. Ereifej, Seth M. Meade, Cara S. Smith, Keying Chen, Nanette Kleinman, Jeffrey R. Capadona

“…These findings are consistent with oral and intravenous administration of propylene glycol toxicity as previously reported in other species, including humans. To our knowledge, this report represents the first published case of status epilepticus due to an IP injection containing propylene glycol.…”
Get full text

Emergence of SARS-CoV-2 subgenomic RNAs that enhance viral fitness and immune evasion. by Harriet V Mears, George R Young, Theo Sanderson, Ruth Harvey, Jamie Barrett-Rodger, Rebecca Penn, Vanessa Cowton, Wilhelm Furnon, Giuditta De Lorenzo, Margaret Crawford, Daniel M Snell, Ashley S Fowler, Anob M Chakrabarti, Saira Hussain, Ciarán Gilbride, Edward Emmott, Katja Finsterbusch, Jakub Luptak, Thomas P Peacock, Jérôme Nicod, Arvind H Patel, Massimo Palmarini, Emma Wall, Bryan Williams, Sonia Gandhi, Charles Swanton, David L V Bauer

“…We show well-supported examples upstream of the Spike gene-within the nsp16 coding region of ORF1b-which is expressed during human infection, and upstream of the canonical Envelope gene TRS, both of which have evolved convergently in multiple lineages. …”
Get full text

Case report: Patients with positive HER-2 amplification locally advanced gastroesophageal junction cancer achieved pathologic complete response with the addition of pembrolizumab t... by Huanhuan Li, Chao Ren, Donghai Cui, Tao Wu, Zhiyong Nie

“…BackgroundHuman epidermal growth factor receptor 2(HER-2) is the most prominent therapeutic target for gastric (G)/gastroesophageal junction (GEJ) cancer. …”
Get full text