Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages by Yongchao Ren, Yunlong Ma, Yanqi Li, Yun Song, WeiWei Zhao, Xuncai Huang, Danmin Yu, Jian Li, Zuogang Xu, Wenjun Zhao

“…Abstract Background Food safety is a significant global study subject that is strongly intertwined with human life and well-being. The utilization of DNA-based methods for species identification is a valuable instrument in the field of food inspection and regulation. …”
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Contribution of the Geodiversity knowledge to social, economic, and environmental health development by Cassio Silva, Edson Farias Mello, Gilberto Dias Calaes, Patricia Duringer Jacques, André Luiz Invernizzi

“…These include mining, where knowledge and proper utilization of essential mineral resources are crucial for human survival and quality of life; energy, which relies on petroleum, gas, coal, peat, and other minerals for nuclear energy generation, as well as for renewable energy sources like wind and solar power. …”
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Study protocol of a pilot randomised controlled trial assessing the feasibility and acceptability of RecoverEsupport: a digital health intervention to enhance recovery in women und... by Christopher Oldmeadow, Mitch J Duncan, Penny Reeves, Priscilla Viana da Silva, Rebecca Wyse, Helen Moore, Alison Zucca, Sancha Robinson, Stephen Ridley Smith, Emma Sansalone, Owen Morris, Rebecca Chenery, Lara Riley, Anna Palazzi-Parsons

“…Data regarding patient behaviours related to patient-managed ERAS recommendations, Quality of Life, European Organization for the Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30), Quality of Recovery (QOR-15), Anxiety (Hospital Anxiety and Depression Scale), hospital readmissions, emergency department presentations and health service utilisation postdischarge will also be collected.Ethics and dissemination This study has been approved by the Human Research Ethics Committees of the Hunter New England Local Health District (2022/ETH02010), the University of Newcastle (H-2023–0298) and the Calvary Mater Newcastle (2022/STE03757). …”
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Greenness and averted mortality in 390 cities in China (2000–2020)Research in context by John S. Ji, Zheng Tao, Hui Miao, Tom Cole-Hunter, Xuecao Li, David Rojas-Rueda, Wenjia Cai

“…Methods: In China, we mapped the urban human population distribution aged 20 and above with a 1 km grid (30 arc-second) and used satellite-based remote sensing to measure green space over time to create population-weighted normalized difference vegetation index (NDVI). …”
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Accuracy of screening tests for cervical precancer in women living with HIV in low-resource settings: a paired prospective study in Lusaka, Zambia by Nicola Low, Partha Basu, Andreas Limacher, Katayoun Taghavi, Misinzo Moono, Mulindi Mwanahamuntu, Herbert Kapesa, Albert Manasyan, Julia Bohlius, Marie Roumet, Thamsanqa Madliwa, Anne Rutjes

“…The index tests were high-risk human papillomavirus detection (hrHPV, Xpert HPV, Cepheid), portable colposcopy (Gynocular, Gynius) and visual inspection with acetic acid (VIA). …”
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Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues by Laís Vicari de Figueiredo Pessôa, Pedro Ratto Lisboa Pires, Maite del Collado, Naira Caroline Godoy Pieri, Kaiana Recchia, Aline Fernanda Souza, Felipe Perecin, Juliano Coelho da Silveira, André Furugen Cesar de Andrade, Carlos Eduardo Ambrosio, Fabiana Fernandes Bressan, Flavio Vieira Meirelles

“…We were able to generate bFGF-dependent iPSCs from eADmsc, eUCmsc, and eFibros with human OSKM, and the miRNA profile revealed that clonal lines may respond differently to the reprogramming process.…”
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Novel Prognostic Factors Associated with Cell Cycle Control in Sporadic Medullary Thyroid Cancer Patients by Raffaele Pezzani, Loris Bertazza, Elisabetta Cavedon, Simona Censi, Jacopo Manso, Sara Watutantrige-Fernando, Gianmaria Pennelli, Francesca Galuppini, Susi Barollo, Caterina Mian

“…Seventy-one sporadic MTC human samples were analyzed for RET mutations and by qPCR for PTTG1 and AURKA (Aurora kinase A) expression. …”
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The microbial mechanism of maize residue decomposition under different temperature and moisture regimes in a Solonchak by Fan Huang, Wan Zhang, Lihua Xue, Bahar Razavi, Kazem Zamanian, Xiaoning Zhao

“…Abstract Soil salinization becomes serious under climate change and human activities. Although the residue decomposition contributes lots to soil carbon storage and fertility, the decomposition process and microbial mechanisms on saline-alkali soils are still vague facing climate change. …”
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Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 by Behrooz Alizadeh Behbahani, Mostafa Rahmati-Joneidabad, Morteza Taki

“…Probiotic bacteria improve human health by secreting pro-microbial substances, balancing intestinal flora, binding to the mucous membrane and epithelium, strengthening the intestinal epithelial barrier, and creating interactions between the gastrointestinal microbiota and the immune system. …”
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Clinicopathological Significance of FOXO4 Expression and Correlation with Prx1 in Head and Neck Squamous Cell Carcinoma by Yunping Lu, Yajun Shen, Lingyu Li, Min Zhang, Min Wang, Lihua Ge, Jing Yang, Xiaofei Tang

“…The function of FOXO4 correlation with HNSCC prognosis was analyzed via ONCOMINE, UALCAN, Human Protein Atlas, and cBioPortal. The expression of FOXO4 was detected in Prx1 silenced CaL27 and SCC9 cell lines by Western blot. …”
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Helicobacter pylori SlyD stabilizes TPT1 via hnRNPK and enhances OCT1-mediated CDX2 transcriptional activation to drive gastric intestinal metaplasia by Shuwen Zheng, Yingying Wang, Chuxuan Ni, Rui Guo, Xunan Qiu, Jijun Chen, Lu Wang, Xiaohu Sun, Moye Chen, Yunen Liu, Yuan Yuan, Yuehua Gong

“…Immunohistochemistry staining was conducted to validate hnRNPK, TPT1, OCT1, and CDX2 expression in gastric tissue samples from both human and Mongolian gerbils. Results H. pylori SlyD upregulates TPT1 and induces the expression of GIM markers through hnRNPK. …”
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Identification of common and specific fibrosis-related genes in three common chronic kidney diseases by Zhangning Fu, Xiaodong Geng, Chao Liu, Wanjun Shen, Zheyi Dong, Guannan Sun, Guangyan Cai, Xiangmei Chen, Quan Hong

“…Therefore, we conducted this study aiming at exploring the common and specific fibrosis-related genes involved in different types of CKD.Methods Kidney biopsy specimens from patients with different types of CKD and normal control subjects were analyzed using the NanoString nCounter® Human Fibrosis V2 Panel. Genes differentially expressed in all fibrotic DN, IgAN and MN tissues compared to the normal controls were regarded as the common fibrosis-related genes in CKD, whereas genes exclusively differentially expressed in fibrotic DN, IgAN or MN samples were considered to be the specific genes related to fibrosis in DN, IgAN and MN respectively. …”
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“If that would have lessened my symptoms, that would have been great...”: a qualitative study about the acceptability of tecovirimat as treatment for mpox by Sara Paparini, Rosalie Hayes, Benjamin Weil, Will Nutland, Ismael Maatouk, Teodora Wi, Chloe M. Orkin, Rosamund Lewis

“…Approval was granted under “exceptional circumstances” based on effectiveness found in pre-clinical challenge studies in animals and safety studies in humans showing minimal side effects. As clinical efficacy studies are still ongoing, there is currently limited information with regard to the acceptability of tecovirimat to treat mpox. …”
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An Analysis of Land Use Conflicts and Strategies in the Harbin–Changchun Urban Agglomeration Based on the Production–Ecological–Living Space Theory and Patch-Generating Land Use Si... by Xiaomeng Wang, Yanjun Zhang, Xiaoyan Li, Shuwen Cao

“…In recent years, rapid economic development, increasing human activities, and global climate change have led to escalating demands for land across production, residential, and ecological domains. …”
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Disease burden of biliary tract cancer in 204 countries and territories, 1990–2021: A comprehensive demographic analysis of the Global Burden of Disease Study 2021 by Xuheng Sun, Jiangmei Liu, Wei Zhang, Yijun Wang, Yan Jiang, Lijun Wang, Yixin Zou, Yuxuan Xiao, Yongbing Xiang, Maolan Li, Maigeng Zhou, Yingbin Liu, Sihan Zhou, Xiuyuan Hao

“…The top three highest incidences, deaths, and DALYs were observed in China, India, and Japan, and the highest ASRs were observed in Chile in 2021. Analysis of the Human Development Index along with disease burden estimates of BTCs also suggests that the burden of the disease is related to the level of comprehensive development of the society. …”
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Functional and Structural Consequences of Nine CYP21A2 Mutations Ranging from Very Mild to Severe Effects by Débora de Paula Michelatto, Leif Karlsson, Ana Letícia Gori Lusa, Camila D’Almeida Mgnani Silva, Linus Joakim Östberg, Bengt Persson, Gil Guerra-Júnior, Sofia Helena Valente de Lemos-Marini, Michela Barbaro, Maricilda Palandi de Mello, Svetlana Lajic

“…Functional analyses were complemented with in silico prediction of mutation pathogenicity based on the recently crystallized human CYP21A2 structure. Mutated proteins were transiently expressed in COS-1 cells and enzyme activities towards 17-hydroxyprogesterone and progesterone were determined. …”
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Contagious acquisition of antimicrobial resistance is critical for explaining emergence in western Canadian feedlots—insights from an agent-based modelling tool by Dana Ramsay, Wade McDonald, Michelle Thompson, Nathan Erickson, Sheryl Gow, Nathaniel D. Osgood, Cheryl Waldner

“…IntroductionAntimicrobial resistance (AMR) is a growing threat to the efficacy of antimicrobials in humans and animals, including those used to control bovine respiratory disease (BRD) in high-risk calves entering western Canadian feedlots. …”
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Genetic variation in patent foramen ovale: a case-control genome-wide association study by Bosi Dong, Yajiao Li, Fandi Ai, Jia Geng, Ting Tang, Wan Peng, Yusha Tang, Hui Wang, Zixuan Tian, Fengxiao Bu, Lei Chen

“…Single-cell sequencing analyses with pseudotime trajectory modeling were employed to evaluate their expression in human fetal hearts.ResultsThe case-control GWAS of discovery cohort ultimately included 517 cases and 517 demographically matched controls. …”
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Effect of Different Cooking Methods on Lipid Content and Fatty Acid Profile of Red Mullet ( Mullus barbatus ) by Francesca Biandolino, Ermelinda Prato, Asia Grattagliano, Isabella Parlapiano

“…The n 3/ n 6 ratio (range 1.25–3.65) decreased significantly after cooking; however, it remained above the recommended values for a healthy human nutrition (1:5). The PUFA/SFA ratio was between 0.69 in fried and 1.02 in boiled fish, both being greater than those recommended by the WHO/FAO [2003]. …”
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METTL1 mediates PKM m7G modification to regulate CD155 expression and promote immune evasion in colorectal cancer by Fang Wang, Chen Yang, Fang Zheng, Yang Yan, Guifang Li, Yanyan Feng, Hejia Xu, Zilong He, Dongyan Cai, Hairong Sun, Xiaowei Qi, Yong Mao

“…Methods The expression levels of Methyltransferase-like 1 (METTL1) and m7G in human tissues were determined. In this study, the effect of METTL1 on RNA m7G levels was evaluated, the effect of METTL1 on PKM mRNA modification was confirmed, the expression level of the PKM2 protein was detected, and the mechanism involved RT‒qPCR, Western blot, RNA stability analysis and RIP analysis. …”
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