Leveraging the fundamentals of heat transfer and fluid mechanics in microscale geometries for automated next-generation sequencing library preparation by Olivia Ott, Sabrina Tolppi, Jennifer Figueroa-Cruz, Khaliun Myagmar, Khulan Unurbuyan, Anubhav Tripathi

“…Our platform successfully prepared eight libraries in parallel, generating sequencing data for both human and Escherichia coli DNA libraries with negligible coverage bias compared to positive controls. …”
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Genetically Engineered Bacterial Ghosts as Vaccine Candidates Against <i>Klebsiella pneumoniae</i> Infection by Svetlana V. Dentovskaya, Anastasia S. Vagaiskaya, Alexandra S. Trunyakova, Alena S. Kartseva, Tatiana A. Ivashchenko, Vladimir N. Gerasimov, Mikhail E. Platonov, Victoria V. Firstova, Andrey P. Anisimov

“…The practical use of BGs in vaccine development for humans is limited because of concerns about the preservation of viable bacteria in BGs. …”
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Occurrence and bioaccumulation of organophosphate flame retardants in high-altitude regions: A comprehensive field survey in Qinghai Province, China by Haibao Zhu, Jinlin Hu, Zheng Ruan, Danhua Liu, Meirong Zhao

“…Organophosphate flame retardants (OPFRs) are a class of substances that pose potential risks to human health and ecosystems due to their large-scale production, wide range of applications, and ubiquitous presence in the environment. …”
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Government and farmer responses to the fall armyworm outbreak in mainland Southeast Asia by Eiichi Kusano, Nipon Poapongsakorn, Urairat Jantarasiri, Kamphol Pantakua, Cuong H. Tran, Khin Akari Tar, Khin Akari Tar, Thong Kong, Viengsavanh V. Phimphachanhvongsod, Youichi Kobori

“…Despite comprehensive recommendations, maize farmers primarily rely on EMB foliar treatment for FAW control.DiscussionWe highlight the need for further research and dissemination regarding the widely accepted foliar application of chemical insecticides, specifically in relation to human safety, improvements in application technology, and clear guidelines for large-scale outbreaks. …”
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Antimicrobials profiling, biofilm formation, and clonal lineage of methicillin-resistant Staphylococcus aureus isolated from cockroaches by Yasin Saberi, Mehrdad Halaji, Mohsen Karami, Jalal Jafarzadeh, Kasra Javadi, Hoda Shirafkan, Abazar Pournajaf

“…Cockroaches are widely recognized as vectors for transmitting pathogenic microorganisms in hospital and community environments due to their movement between contaminated and human-occupied spaces. Staphylococcus aureus (S. aureus), particularly methicillin-resistant Staphylococcus aureus (MRSA), is a primary global health concern because of its capacity to cause a wide range of infections and its resistance to many antibiotics. …”
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Characteristics of the smallest brucellaphage with strong lytic ability by Hongbaiyu Liu, Hongbaiyu Liu, Youhong Zhong, Zhihong Zhang, Kehong Xu, Kehong Xu, Chunpeng Mao, Chunpeng Mao, Qiuju Yang, Lihua Yang, Binbin Yu, Ying Long, Ying Long, Xinyu Qin, Xinyu Qin, Liyuan Shi, Sheng Chang, Yuanying Shen, Peng Wang

“…Brucellosis is a globally prevalent zoonotic disease caused by Brucella spp. posing significant threats to animal and human health. In this study, a novel lytic brucellaphage designated Y17 was isolated from sheep fecal samples collected in Ludian County, Yunnan Province, China. …”
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Predicting positive Clostridioides difficile test results using large-scale longitudinal data of demographics and medication history by Anh Pham, Robert El-Kareh, Frank Myers, Lucila Ohno-Machado, Tsung-Ting Kuo

“…There is a general consensus among different analysis approaches regarding predictors that impact patients' chances of having a positive test, which may influence Clostridioides difficile risk, including features clinically proven to increase susceptibility. These human-interpretable models can help distinguish significant predictors that affect a patient's chance of testing positive, which may influence their Clostridioides difficile risk.…”
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Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer by Viktoriya N. Shumilova, Artemij E. Goncharov, Daniil V. Azarov, Stanislav I. Sitkin, Elgudzha L. Latariya, Batyrbek I. Aslanov, Mikhail A. Bobrakov, Rustem E. Topuzov

“…A number of recent studies discuss the relationship between the disease and various potentially oncogenic microorganisms in the human intestinal tract, which can be used to expand the arsenal of non-invasive methods for diagnosing CRC based on molecular genetic examination of a stool sample to identify oncogenic microorganisms. …”
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On the tracks of an uninvited guest, the Asian tiger mosquito, Aedes albopictus in Cyprus by Riccardo Piccinno, Giulia Fiorenza, Marlen Ines Vasquez, Jeremy Bouyer, Gregoris Notarides, Ludvik Marcus Gomulski, Soteris Meletiou, Mustafa Akiner, Antonios Michaelakis, Federico Forneris, Giovanni Maga, Giuliano Gasperi, Anna Rodolfa Malacrida

“…This mosquito species has rapidly dispersed and invaded much of the globe assisted by its life history traits and high propagule pressure driven by human activities. Aedes albopictus is currently widespread across mainland Europe and the Mediterranean region, including the islands. …”
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Arbovirus vectors of epidemiological concern in the Americas: A scoping review of entomological studies on Zika, dengue and chikungunya virus vectors. by Reilly Jones, Manisha A Kulkarni, Thomas M V Davidson, RADAM-LAC Research Team, Benoit Talbot

“…<h4>Background</h4>Three arthropod-borne viruses (arboviruses) causing human disease have been the focus of a large number of studies in the Americas since 2013 due to their global spread and epidemiological impacts: Zika, dengue, and chikungunya viruses. …”
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The relationship between oxLDL, sLOX-1, PCSK9 and carotid intima-media thickness in patients with prediabetes and type 2 diabetes by Zeki Dogan, Abdulhalim Senyigit, Sinem Durmus, Canan Duvarcı, Remise Gelişgen, Hafize Uzun, Omur Tabak

“…Measurement of serum oxLDL, sLOX-1 and PCSK9 levels were assessed by using a commercially available human enzyme-linked immunosorbent assay (ELISA) method. …”
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A prospective study to evaluate the clinical specificity of the cobas® MPX test kit for screening for HIV RNA, HCV RNA, and HBV DNA in blood donation samples using the cobas® 6800... by Lei Zhou, Lin Wang, Xiaofang Gong, Xiaochun Liu, Yaxuan Zou, Yingying Wang, Jinfeng Zeng, Liang Zang

“…Roche's advanced cobas® MPX assay detects human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) using the cobas® 6800/5800 Systems, based on real-time PCR technology, providing improved sensitivity. …”
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Ecological security driving mechanisms and optimization of zoning in Chinese urban agglomerations: A case study of the central plains urban agglomeration by Jinyuan Zhang, Xuning Qiao, Yongju Yang, Liang Liu, Yalong Li, Shengnan Zhao

“…Spatial disparities in ecological security were relatively small, and counties with lower ecological security levels tended to show greater clustering; (2) The explanatory power of the driving factors is ranked as follows: human factors > natural factors > landscape factors. …”
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Optimization of the Solid-state Fermentation Process for Morchella esculenta Fermented Wheat and Analysis of Its Nutritional Components, Physicochemical Properties and Antioxidant... by Dandan GU, Xue DONG, Jinxiu ZHANG, Xiaoru WANG, Zongshuo ZHAO, Li'an WANG

“…Under simulated conditions of the human stomach and intestine, the cholesterol adsorption rate increased by 86.29% and 290.14%, respectively. …”
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The paediatric liver transplant experience in Johannesburg, South Africa: A broad overview and update by K Kinandu, A Beeton, M Beretta, S Berkenfeld, L Brannigan, R Britz, D Demopoulos, L Doedens, M Duncan, P Gaylard, C Hajinicolaou, W Lowman, H Maher, T De Maayer, V Mudau, S Rambarran, M Reynders, F van der Schyff, B Ströbele, S Tager, E Wessels, J Fabian, J Loveland

“…Data were accessed from the Wits Donald Gordon Medical Centre Paediatric Liver Transplant Research Database (University of the Witwatersrand Human Research Ethics approval: M190749). The following data were collected: donor and recipient sociodemographic and clinical characteristics, details of transplant procedures, donor grafts and recipient outcomes (post-operative complications, graft and recipient survival).   …”
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Entre la creatividad y el recuerdo: el espacio de juego en el arte de instalación de Carsten Höller y Gelitin by Javier Gómez Chozas, Vanesa Cejudo Mejías, Arturo Franco Díaz

“…Tomando como ejemplo las obras Test Site (2006), del artista alemán Carsten Höller, y Human Elevator (1999-2016), del colectivo austríaco Gelitin, se comparan las obras para llegar a diversas conclusiones acerca de la inserción del juego infantil en la programación de exposiciones en las instituciones artísticas. …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…In addition to standard supplementation given by foetal bovine serum (FBS) or human platelet lysate (hPL), new good manufacturing practice (GMP)-compliant serum/xeno (S/X)-free media formulations have been proposed, although their influence on MSCs phenotype and potential is scarcely described. …”
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Predictors of treatment failure of tubal pregnancy with single-dose methotrexate regimen – a systematic review and meta-analysis by Lili Tang, Sipei Nie, Ling Ling, Qian Zhu

“…This meta-analysis aimed to identify early predictors for single-dose methotrexate (MTX) treatment failure in tubal pregnancies.Methods A literature search was conducted across several databases from their inception to December 2023, with references in the selected studies manually reviewed. 14 studies involving 2,804 patients were included in this meta-analysis.Results The results revealed that higher serum beta-human chorionic gonadotropin (β-hCG) levels on Day 1 (SMD = 1.25, 95% CI 0.73–1.77), foetal cardiac activity presence (OR = 12.64, 95% CI 3.15–50.75), adnexal mass presence (OR = 4.66, 95% CI 2.02–10.74), yolk sac presence (OR = 5.35, 95% CI 2.33–12.27), thicker endometrium (MD = 1.74, 95% CI 0.30–3.19), more number of previous ectopic pregnancies (MD = 0.21, 95% CI 0.13–0.30), history of pelvic inflammatory disease (PID) (OR = 3.97, 95% CI 2.02–7.79), higher progesterone levels on Day 1 (SMD = 0.22, 95% CI 0.07–0.36), a higher 48-hour pre-treatment increment in serum β-hCG percentage (MD = 11.46, 95% CI 2.95–19.98), and a higher percentage of serum β-hCG change from Day 4 to Day 0/1 (SMD = 2.58, 95% CI 1.02–4.14) were early predictive factors for treatment failure of tubal pregnancy with the MTX single-dose regimen.Conclusions This review clarifies early predictive factors for treatment failure with the MTX single-dose regimen in tubal pregnancies. …”
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Shenshuaikang enema restores the intestinal barrier and microbiota-gut-kidney axis balance to alleviate chronic kidney disease via NF-κB pathway by Yan Ye, Yan Ye, Xiaopeng Huang, Xiaopeng Huang, Xiaopeng Huang, Xueying Li, Xueying Li, Fei Gao, Wenzhen Zhong, Anqi Tang, Anqi Tang, Liangbin Zhao, Dengpiao Xie, Naijing Ye, Naijing Ye

“…It has become a significant public health problem, posing a threat to human health worldwide. Shenshuaikang enema (SSKE) has demonstrated notable efficacy and safety in treating CKD, although its mechanism of action remains unclear.MethodsThe CKD rat model was induced using 2.5% adenine, and the effect of SSKE was evaluated by detecting uremic toxins, inflammatory cytokines, and renal function. …”
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Concept of a geoinformation platform for landmines and other explosive objects detection and mapping with UAV by Mykhailo Popov, Sergey Stankevich, Sergey Mosov, Stanislav Dugin, Stanislav Golubov, Artem Andreiev, Artur Lysenko, Ievgen Saprykin

“…Undetected explosives threaten business (agriculture, logistics, etc.) and human lives. The problem becomes more acute with the rapid extension of minefield areas, which requires significant time and resources and carries high risks. …”
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