Integrated metabolome and transcriptome analysis reveals key genes and pathways associated with egg yolk percentage in chicken by Wen Li, Yuxing Luo, Shoujia Zhu, Mengyuan Wang, Xuli Zhao, Zhonghua Ning

“…To elucidate the underlying mechanisms that contribute to variations in egg yolk percentage, we performed integrated transcriptome and metabolome analyses on the liver, ovary, and magnum tissues of Rhode Island Red chickens with high and low yolk percentages. A total of 322 differentially expressed genes (DEGs) and 128 significantly differential metabolites (SDMs) (VIP>1, P < 0.05) were identified in the liver, whereas 419 DEGs and 215 SDMs were detected in the ovary, and 238 DEGs along with 47 SDMs were found in the magnum. …”
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Pooling the complex survey data across the 64 lower and middle-income countries: A study on antibiotic usage in under-five children by Md Fakrul Islam, Prosenjit Basak Arka, Mahfuzer Rohman, Md Sabbir Hossain, Md Rashed Babu, Hasin Anupama Azhari, Md Jamal Uddin

“…The researchers recommend that the countries with high and low percentages of antibiotic exposure investigate either any possibility of antibiotic abuse or proper healthcare service at the national level.…”
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Whether Detection of Gene Mutations Could Identify Low- or High-Risk Papillary Thyroid Microcarcinoma? Data from 393 Cases Using the Next-Generation Sequencing by Lei Jin, Liang Zhou, Jian-Biao Wang, Li Tao, Xiao-Xiao Lu, Na Yan, Qian-Ming Chen, Li-Ping Cao, Lei Xie

“…The objective of this study is to explore the utilization of next-generation sequencing (NGS) technology in evaluating the likelihood of identifying individuals with papillary thyroid microcarcinoma (PTMC ≤10 mm) who are at high or low risk. Design. NGS was used to analyze 393 formalin-fixed, paraffin-embedded tissues of PTC tumors, all of which were smaller than 15 mm. …”
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The prognostic value of systemic immune-inflammation index in patients with unresectable hepatocellular carcinoma treated with immune-based therapy by Tian He, Bin Xu, Lu-Na Wang, Zi-Yi Wang, Huan-Chen Shi, Cheng-Jie Zhong, Xiao-Dong Zhu, Ying-Hao Shen, Jian Zhou, Jia Fan, Hui-Chuan Sun, Bo Hu, Cheng Huang

“…Results An optimal SII cutoff of 752*109 stratified patients into high and low SII groups. Univariate and multivariate logistic regression indicated that SII was a significant predictor of the objective response rate (ORR), which was markedly different between the low and high SII subgroups (34.72% vs. 9.52%, P = 0.019). …”
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Single-cell transcriptomics reveals the heterogeneity and function of mast cells in human ccRCC by Xiyu Song, Xiyu Song, Xiyu Song, Jianhua Jiao, Jianhua Jiao, Jiayang Qin, Wei Zhang, Weijun Qin, Weijun Qin, Shuaijun Ma, Shuaijun Ma

“…Activated and resting MCs were defined as having high and low expression of MC receptors and mediators, respectively, whereas proliferating MCs had high expression of proliferation-related genes. …”
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A Study of NOAA-20 VIIRS Band M1 (0.41 µm) Striping over Clear-Sky Ocean by Wenhui Wang, Changyong Cao, Slawomir Blonski, Xi Shao

“…However, NOAA-20 operational M1 (a dual gain band with a center wavelength of 0.41 µm) sensor data records (SDR) have exhibited persistent scene-dependent striping over clear-sky ocean (high gain, low radiance) since the beginning of the mission, different from other VisNIR bands. …”
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Reliability and validity of the International Classification of Functioning，Disability and Health Rehabilitation set in the Rehabilitation of Knee Osteoarthritis by LEI Lei, PENG Zhizhou, GAO Mingyue, LI Fei, MAO Ningning, WU Xi, XIAO Chengcheng, XUN Yajing, ZHAO Suchen, FAN Xiaojun

“…The 70. 00% （21/30） items were significantly different in distinguishing between mild and severe dysfunction, in which items with r&gt;0. 8 could effectively distinguish KOA patients with high and low score functions, including d420 moving oneself, d465 using equipment for moving around, d510 washing oneself, d640 doing housework, and d470 using transportation.ConclusionICF-RS has a good reliability, validity and criterion-related validity, and can be used to assess the functional status of KOA patients.…”
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Predictive Validity and Reliability of Frenchay Activities Index by Li TONG, Shanjia CHEN, Guanli XIE, Cai JIANG, Jing TAO, Lidian CHEN

“…Ninety-four subjects were randomly selected within one week and retested by the same assessor.Results:The internal consistency of the Chinese version of the FAI scale was Cronbach's α coefficient of 0.937.The test-retest reliability showed that the intra-group correlation coefficient(ICC)was 0.810(95%<italic>CI</italic>:0.672 to 0.952).In terms of project discrimination, the differences between the high and low group scores of each project were statistically significant(<italic>P</italic>&lt;0.01), and the discrimination of each item in the scale was generally good;the correlation analysis of the entries, dimensions and total scores of the Chinese version of the FAI scale found that the correlation coefficient between the items in the Chinese version of FAI and the total score was in the range of 0.535-0.832(<italic>P</italic>&lt;0.01), and the correlation coefficient of the three dimensions and total scores of household activities, leisure activities and outing activities were 0.867, 0.913, 0.948 respectively(<italic>P</italic>&lt;0.01).The exploratory factor analysis(EFA)was used to test the structural validity of the Chinese version of the FAI scale to a KMO statistic of 0.924 and the Bartlett spherical χ²test to 4535.558(<italic>df</italic>=105, <italic>P</italic>&lt;0.01).Using the principal component analysis method to extract the common factors from the scale, there were three common factors with eigenvalue≥1, the eigenvalue of common factor 1 was 4.776, the contribution rate of variance was 31.842%, and the eigenvalue of common factor 2 was 3.437.The contribution rate of variance was 22.914%, the eigenvalue of common factor 3 was 2.436, the contribution rate of variance was 16.238%, and the cumulative contribution of the three common factors to the total variance was 70.994%.The validity-related validity test showed that the FAI total score was positively correlated with the MBI total score, the correlation was general(<italic>r</italic>=0.322, <italic>P</italic>&lt;0.01), and the three dimensions of FAI(home activities, leisure activities, outing activities)was correlated with the MBI total score, the correlation was general(<italic>r</italic>=0.320, <italic>r</italic>=0.267, <italic>r</italic>=0.319, <italic>P</italic>&lt;0.01).Conclusion:The Chinese version of the Frenchay activities index has good reliability and validity in patients with recovery from stroke hospitalization.…”
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Cecum microbiota composition, fermentation characteristics, and immunometabolic biomarkers of Yunshang black goat fed varying dietary energy and protein levels by Binlong Fu, Xiaoqi Zhao, Muhammad Khan, Yanting Jiang, Weijuan Li, Maida Mushtaq, Baiji Danzeng, Xiaojun Ni, Zobia Azeem, Qingyong Shao, Bai Xue, Yina Ouyang

“…Moreover, it is well known that changes in dietary regimens including nutrient levels result in varied gut microbiota composition, and ultimately, the performance and health of these animals.MethodsThe current study examined the effects of varying dietary energy and protein levels on the cecal fermentation, immune biomarkers, and microbiota characteristics of 80 male Yunshan Black Goats (6 months, ~35.82 ± 2.79 kg), divided into four diets: 1) High Energy-High Protein (HEHP), 2) High Energy-Low Protein (HELP), 3) Low Energy-High Protein (LEHP), and 4) Low Energy-Low Protein (LELP). …”
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Integration and Comparative Analysis of Remote Sensing and In Situ Observations of Aerosol Optical Characteristics Beneath Clouds by Jing Chen, Jing Duan, Ling Yang, Yong Chen, Lijun Guo, Juan Cai

“…Lu reveals the following: (1) The AOD seasonal distribution frequencies under both clear-sky and cloudy-sky conditions are unimodal, with a values of 0.2∼0.6, and the inhomogeneity of the aerosol distribution in winter is evident; the seasonal difference in the AOD under clear-sky conditions is more significant, following the order of spring > summer > winter > autumn, and the AOD seasonal difference under cloudy-sky conditions is not obvious. (2) In the analysis of the AOD monthly variations, due to the influence of the meteorological conditions (high humidity, low wind speed) and pollutant transport, the AOD reached its peak in February (clear-sky: 0.63, cloudy-sky: 0.82). (3) Under clear-sky conditions, the negative correlation between the daily variations in AOD, and visibility is more significant during the daytime, and after 12:00, the AOD is positively correlated with <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>P</mi><msub><mi>M</mi><mrow><mn>2.5</mn></mrow></msub></mrow></semantics></math></inline-formula>; these results indicate that the AOD is affected mainly by pollutants and the boundary layer height. …”
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Comparative Analysis of Multiple Hydrological Models in Assessing Climate Change Impacts on the Mille Watershed, Awash Basin, Ethiopia by Asmare Belay Nigussie, Mihiretie Ayana, Dessalegn Worku Ayalew, Gerawork Feleke, Asnake Enawgaw Amognehegn, Anteneh Yayeh Adamu, Andualem Endalew, Tewodrose Desale Meselaw, Shankar Karuppannan

“…The significance of this study lies in its contribution to understanding climate-hydrology interactions in a vulnerable region, providing actionable insights for adaptation planning, policy formulation, and sustainable resource management in the face of climate change. Extreme high and low changes in flow were used to quantify this impact. …”
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Status and influencing factors of health literacy among college students of traditional Chinese medicine: a cross-sectional study by Jie Zhu, Cuixia Lin, Yanhui Yang, Rui Yao, Ran Yang, Mingqian Jiang

“…Among the three aspects of health literacy, the level from high to low was: healthy lifestyle and behavior literacy (65.41%), health skill literacy (58.70%) and basic knowledge and concept literacy (53.62%). …”
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Protective Effects of Gynostemma pentaphyllum Saponins on Liver Injury in Guinea Pigs of Hyperlipemia Based on Oxidative Stress by Tianyi DAI, Donglian LIU, Xingmin QIN, Chengling GAO, Jiayi LI, Hailing KUANG

“…The fluorescence expression of Nrf2 and HO-1 proteins in both the high and low dose groups of GPs significantly increased (P<0.01). …”
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Qualitative Insights on Barriers to Receiving a Second Dose of Measles-Containing Vaccine (MCV2), Oromia Region of Ethiopia by Kalkidan Solomon, Brooke N. Aksnes, Abyot Bekele Woyessa, Chala Gari Sadi, Almea M. Matanock, Monica P. Shah, Paulos Samuel, Bekana Tolera, Birhanu Kenate, Abebe Bekele, Tesfaye Deti, Getachew Wako, Amsalu Shiferaw, Yohannes Lakew Tefera, Melkamu Ayalew Kokebie, Tatek Bogale Anbessie, Habtamu Teklie Wubie, Aaron Wallace, Ciara E. Sugerman, Mirgissa Kaba

“…We selected ten woredas (districts) in the Oromia Region, Ethiopia, stratified by settlement type (urban/rural), MCV1 coverage (high ≥ 80%; low < 80%), and history of measles outbreaks between June 2019 and June 2020. …”
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Microplastic analysis in soils: A comparative assessment by Stoyana Peneva, Quynh Nhu Phan Le, Davi R. Munhoz, Olivia Wrigley, Flora Wille, Heidi Doose, Crispin Halsall, Paula Harkes, Michael Sander, Melanie Braun, Wulf Amelung

“…Each technique was coupled with a specific extraction procedure and evaluated for three soils with different textures and organic carbon contents, amended with eight types of large MiPs (0.5–1 mm) – high- and low-density polyethylene (HDPE and LDPE), polypropylene (PP), polystyrene (PS), polyamide (PA), polyethylene terephthalate (PET), polyvinyl chloride (PVC), and a biodegradable mulch film product composed of polybutylene adipate-co-terephthalate/ polylactic acid (PBAT/ PLA). …”
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Serum advanced glycation end products as a putative biomarker in Type2 DKD patients’ prognosis by Ze-Hou Wang, Ze-Hou Wang, Zong-Jin Zhang, Yue-Fen Wang, Jin Xie, Jin Xie, Yi-Min Li, Cun Shen, Yuan Meng, Wen-Jing Zhao, Lu-Ying Sun, Wei Jing Liu

“…Participants were stratified into the high- and low-level groups based on the median corrected lgAGEs. …”
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An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers by Ruyi Liu, Ruyi Liu, Ruyi Liu, Ruyi Liu, Ruyi Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Weicheng Huang, Weicheng Huang, Weicheng Huang, Weicheng Huang, Weicheng Huang, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng

“…The drug sensitivity analysis revealed that patients with high or low Anoscores were sensitive to different chemotherapies and targeted drugs. …”
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Four-dimensional flow magnetic resonance imaging for noninvasive diagnosis of clinically significant portal hypertension and high-risk gastroesophageal varices in patients with cir... by Jiachen Ji, Yi Xiang, Jinghui Dong, Hanyu Jiang, Tianyi Xia, Yunduo Li, Mengmeng Zhang, Changchun Liu, Xijun Gong, Bo Jin, Wen Shen, Gangfeng Zhu, Jianming Cai, Rui Li, Xiaolong Qi

“…Hemodynamic parameters within the portal vein, including the average and peak flow velocities, normalized flow volume (Qnormal), and regurgitant fraction (R%), were extracted and compared between healthy individuals and patients with CSPH and between participants with high- and low-risk varices. Subsequently, these parameters were incorporated into a logistic regression (LR) model refined using L1 regularization and validated using five-fold cross-validation. …”
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Identification and validation of a prognostic signature of drug resistance and mitochondrial energy metabolism-related differentially expressed genes for breast cancer by Tiankai Xu, Chu Chu, Shuyu Xue, Tongchao Jiang, Ying Wang, Wen Xia, Huanxin Lin

“…Furthermore, we examined immune infiltration and tumor mutation burden of the genes within our model and scrutinized divergences in the immune microenvironment between high- and low-risk groups. Small hairpin RNA and lentiviral plasmids were designed for stable transfection of breast cancer cell lines MDA-MB-231 and HCC1806. …”
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Identification and validation of BATF as a prognostic biomarker and regulator of immune cell infiltration in acute myeloid leukemia by Zhe Zhao, Dongmei Wang, Dongmei Wang, Xue Sheng, Shuying Li, Tingting Liu, Mengyuan Chang, Lei Feng, Di Zhang, Chunyan Ji, Chunyan Ji, Fei Lu, Jingjing Ye

“…Finally, there were differences in receptor ligand pairs between AML cells with high and low expression of BATF and immune cells.ConclusionBioinformatics analysis and experimental verification revealed that BATF expression could alter the proportions of CD8+T cells and NK cells in AML and affect drug sensitivity, making it a potential treatment target for AML.…”
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