A novel truncated fusion gene ETV6::AC010198.2 in post-MPN acute myeloid leukemia caused by del(12)(p13p11) by Jiao Lu, Jiao Lu, Guanqun Yang, Qiurong Zhang, Man Wang, Man Wang, Lijun Wang, Lijun Wang, Lu Chen, Haoyue Chen, Xin Jiang, Chunhua Liu, Tong Lin, Qiaoyan Han, Zefa Liu, Shi Zhen, Zhao Zeng, Jinlan Pan, Jiannong Cen, Suning Chen, Suning Chen, Zheng Wang, Zheng Wang, Xingxia Zhang, Miao Sun

“…The gene ETV6 has been confirmed to be a genetic susceptibility gene for thrombocytopenia and leukemia. Here, we report a long-chain noncoding RNA AC010198.2 as a novel fusion partner of ETV6, showing a karyotype of del(12)(p13p11), with poor prognosis in a post-MPN AML that has never been reported, which may be an vital initial event in the transformation of MPN to AML and deterioration of disease.…”
Get full text

Biomarker combinations from different modalities predict early disability accumulation in multiple sclerosis by Vinzenz Fleischer, Tobias Brummer, Muthuraman Muthuraman, Muthuraman Muthuraman, Falk Steffen, Milena Heldt, Maria Protopapa, Muriel Schraad, Gabriel Gonzalez-Escamilla, Sergiu Groppa, Stefan Bittner, Frauke Zipp

“…ObjectiveEstablishing biomarkers to predict multiple sclerosis (MS) disability accrual has been challenging using a single biomarker approach, likely due to the complex interplay of neuroinflammation and neurodegeneration. Here, we aimed to investigate the prognostic value of single and multimodal biomarker combinations to predict four-year disability progression in patients with MS.MethodsIn total, 111 MS patients were followed up for four years to track disability accumulation based on the Expanded Disability Status Scale (EDSS). …”
Get full text

High-accuracy crRNA array assembly strategy for multiplex CRISPR by Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang

“…However, a streamlined, practical strategy for CRISPR array assembly that is both convenient and accurate is lacking. Here, we present a novel, highly accurate, cost-, and time-saving strategy for CRISPR array assembly. …”
Get full text

Enhanced interannual variability of the terrestrial carbon sink in China under high emissions by Han Wu, Li Zhang, Honglin He, Xiaoli Ren, Mengyu Zhang, Dingjin Chu, Yuanhao Zheng

“…However, how these future changes will modulate the IAV of China’s terrestrial carbon sinks remains unclear. Here, we analyzed the IAV of China’s terrestrial net ecosystem productivity (NEP _IAV ) and investigated the potential impacts of climate change under various scenarios during the 21st century using the outputs from nine Coupled Model Intercomparison Project Phase 6 models. …”
Get full text

Multi-decade northward shift of loggerhead sea turtle pelagic habitat as the eastern North Pacific Transition Zone becomes more oligotrophic by Dana K. Briscoe, Dana K. Briscoe, Larry B. Crowder, George H. Balazs, Jeffrey A. Seminoff, Alberto Abreu, Catherine A. Lee Hing, Masanori Kurita, Masanori Mori, Denise M. Parker, Marc R. Rice, Tomomi Saito, Bianca S. Santos, Calandra N. Turner Tomaszewicz, Noah Yamaguchi, Jeffrey J. Polovina

“…This has left a critical gap in our understanding of long-term (decadal or longer) change and species’ responses within the NPTZ. Here, we integrate nearly 3 decades of satellite tracking data from a climate sentinel, the juvenile North Pacific loggerhead sea turtle (Caretta caretta), with concurrent observations of sea surface temperature (SST) and chlorophyll-a concentrations to examine higher trophic level response to climate-induced changes within the eastern bounds of the NPTZ. …”
Get full text

Modification and analysis of context-specific genome-scale metabolic models: methane-utilizing microbial chassis as a case study by M. A. Kulyashov, R. Hamilton, Y. Afshin, S. K. Kolmykov, T. S. Sokolova, T. M. Khlebodarova, M. G. Kalyuzhnaya, I. R. Akberdin

“…However, the application of CS modeling for non-conventional microbes is still challenging. Here, we present a graphical user interface that integrates COBRApy, EscherPy, and RIPTiDe, Python-based tools within the BioUML platform, and streamlines the reconstruction and interrogation of the CS genome-scale metabolic frameworks via Jupyter Notebook. …”
Get full text

Recent methane surges reveal heightened emissions from tropical inundated areas by Xin Lin, Shushi Peng, Philippe Ciais, Didier Hauglustaine, Xin Lan, Gang Liu, Michel Ramonet, Yi Xi, Yi Yin, Zhen Zhang, Hartmut Bösch, Philippe Bousquet, Frédéric Chevallier, Bogang Dong, Cynthia Gerlein-Safdi, Santanu Halder, Robert J. Parker, Benjamin Poulter, Tianjiao Pu, Marine Remaud, Alexandra Runge, Marielle Saunois, Rona L. Thompson, Yukio Yoshida, Bo Zheng

“…Abstract Record breaking atmospheric methane growth rates were observed in 2020 and 2021 (15.2±0.5 and 17.8±0.5 parts per billion per year), the highest since the early 1980s. Here we use an ensemble of atmospheric inversions informed by surface or satellite methane observations to infer emission changes during these two years relative to 2019. …”
Get full text

Bifidobacterium fermentation with infant formulas is associated with benefits for gut and brain barrier function by Emily G. Knox, Paula Sánchez-Díaz, Colin Buttimer, Sarah-Jane Leigh, Klara Vlckova, Colin Hill, Elaine Kennedy, Jonathan O’Regan, Seamus McSweeney, David Goulding, Maria Rodriguez Aburto, Caitriona M. O’Driscoll, John F. Cryan, Eoin Gunnigle, Gerard Clarke

“…Bifidobacterium is the predominant bacteria in the infant gut microbiome. Here, we evaluated the impact of four infant formulas fermented with three Bifidobacterium strains, originally isolated from infant stool, on gut and BBB integrity. …”
Get full text

Light intensity-dependent arrestin switching for inactivation of a light-sensitive GPCR, bistable opsin by Baoguo Shen, Seiji Wada, Tomohiro Sugihara, Takashi Nagata, Haruka Nishioka, Emi Kawano-Yamashita, Takeaki Ozawa, Mitsumasa Koyanagi, Akihisa Terakita

“…The amount of visible light-sensitive active states (photoproducts) is crucial for generating color opponency, raising questions about how and what arrestins are involved in PP1 inactivation. Here, we found two arrestins, Arr3a and Sagb competitively bind to PP1. …”
Get full text

Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice by Xian’an Yu, Qiuqin Zhou, Xin-Yuan Huang, Mingxue Chen, Jiawen Zhou, Tong Zhou, Pengjie Hu, Xia Pan, Miaomiao Cheng, Yongming Luo, Peter Christie, Longhua Wu

“…However, the impacts of reduced manganese (Mn) uptake caused by OsNRAMP5 mutation on rice growth and Cd accumulation under varying Mn levels remains unknown. Here, hydroponics and pot experiments were used to investigate the growth and Cd accumulation of an OsNRAMP5 mutant rice, Zhong’an 7 (ZA7), and the wild type, Zhongzao 35 (ZZ35), under different Mn supply conditions, and the Mn thresholds for ZA7 were determined. …”
Get full text

USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer by Fang Yuan, Juan Xu, Lingmei Xuan, Chan Deng, Wei Wang, Rong Yang

“…Moreover, USP14 knockdown significantly restrained the proliferation and inhibited the radiation tolerance of ESCC cells. Here, we identified a potential inhibitor of USP14, Degrasyn (DGS), and investigated its regulatory effects on ESCA radioresistance and progression. …”
Get full text

Posterior and Anterior Spinal Fusion for the Management of Deformities in Patients with Parkinson's Disease by Masashi Sato, Takeshi Sainoh, Sumihisa Orita, Kazuyo Yamauchi, Yasuchika Aoki, Tetsuhiro Ishikawa, Masayuki Miyagi, Hiroto Kamoda, Miyako Suzuki, Gou Kubota, Yoshihiro Sakuma, Kazuhide Inage, Yasuhiro Oikawa, Junichi Nakamura, Masashi Takaso, Gen Inoue, Tomoaki Toyone, Kazuhisa Takahashi, Seiji Ohtori

“…In particular, it is difficult to manage deformity correction surgery for patients with Parkinson's disease. Here, we present two cases of combined anterior and posterior surgery for deformity in patients with adult scoliosis and kyphosis due to Parkinson's disease. …”
Get full text

Self-assembled hole-selective contact for efficient Sn-Pb perovskite solar cells and all-perovskite tandems by Jingwei Zhu, Xiaozhen Huang, Yi Luo, Wenbo Jiao, Yuliang Xu, Juncheng Wang, Zhiyu Gao, Kun Wei, Tianshu Ma, Jiayu You, Jialun Jin, Shenghan Wu, Zhihao Zhang, Wenqing Liang, Yang Wang, Shengqiang Ren, Changlei Wang, Cong Chen, Jinbao Zhang, Dewei Zhao

“…Yet most of SAMs are largely suitable for pure Pb-based devices, precisely developing promising hole-selective contacts (HSCs) for Sn-based PSCs and exploring the underlying general mechanism are fundamentally desired. Here, based on the prototypical donor-acceptor SAM MPA-BT-BA (BT), oligoether side chains with different length (i.e., methoxy, 2-methoxyethoxy, 2-(2-methoxyethoxy)ethoxy group) were custom-introduced on the benzothiadiazole unit to produce the target SAMs with acronyms MPA-MBT-BA (MBT), MPA-EBT-BA (EBT), and MPA-MEBT-BA (MEBT), respectively, and acting as HSCs for efficient Sn-Pb PSCs and all-perovskite tandems. …”
Get full text

Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment by Tatsuya Usui, Masashi Sakurai, Shuhei Enjoji, Hideyoshi Kawasaki, Koji Umata, Takashi Ohama, Nobuyuki Fujiwara, Ryotaro Yabe, Shunya Tsuji, Hideyuki Yamawaki, Shoichi Hazama, Hiroko Takenouchi, Masao Nakajima, Ryouichi Tsunedomi, Nobuaki Suzuki, Hiroaki Nagano, Koichi Sato

“…However, ALI organoid culture system from tissues of colorectal cancer patients has not been established. Here, we developed an ALI organoid model from normal and tumor colorectal tissues of human patients. …”
Get full text

Canopy structural heterogeneity drives α and β species–genetic diversity correlations in a Chinese subtropical forest by Zhiliang Yao, Xia Pan, Xin Yang, Xiaona Shao, Bin Wang, Yun Deng, Zhiming Zhang, Qiaoming Li, Luxiang Lin

“…However, few studies have examined the role of canopy structural heterogeneity, which is a defining feature of forests, in shaping SGDCs. Here, we determine what factors contribute to α- and β-species–genetic diversity correlations (i.e., α- and β-SGDCs) in a Chinese subtropical forest. …”
Get full text

Liver-specific gene PGRMC1 blocks c-Myc-induced hepatocarcinogenesis through ER stress-independent PERK activation by Fubo Ji, Jianjuan Zhang, Liping Mao, Yaqi Tan, Meihua Ye, Xianglei He, Yongzhi Zhao, Jiaxin Liu, Yan Zhang, Nachuan Zhang, Jiong Shi, Jianing Yan, Xiujun Cai, Bin Zhao, Jianping Jin, Pinglong Xu, Stephanie Roessler, Xin Zheng, Junfang Ji

“…Abstract Roles of liver-specific genes (LSGs) in tumor initiation and progression are rarely explored in hepatocellular carcinoma (HCC). Here we show that LSGs are generally downregulated in HCC tumor tissues compared to non-HCC liver tissues, and low-LSG HCCs show poor prognosis and the activated c-Myc pathway. …”
Get full text

Genetic investigations of autosomal recessive inherited ichthyosis impressed by fetal ultrasound: Exome sequencing and haplotype linkage analysis by Ting-Yu Chang, Zhu Wei Lim, Yi-Tzu Chu, Wan-Ju Wu, Mei-Hui Lee, Shun-Ping Chang, Dong-Jay Lee, Wen-Hsiang Lin, Ming Ho, Jin-Chung Shih, Gwo-Chin Ma, Ming Chen

“…The prenatal diagnosis of ichthyosis is challenged due to the clinical and genetic heterogeneity and the late-onset of fetal features on ultrasound scan. Here, we reported two fetuses with Harlequin ichthyosis (HI), a severe subtype of autosomal recessive congenital ichthyosis (ARCI), who were diagnosed prenatally by images and genetic investigations. …”
Get full text

Quantitative attribution of 2016 extreme arctic warmth: comparison between late winter and early spring by Junjie Zhu, Ke Fan, Shengping He, Tuantuan Zhang, Yi Deng, Song Yang, Deliang Chen, Kaiqiang Deng, Wei Yu, Baoqiang Tian, Hoffman H N Cheung

“…A deep, large-scale warmth occurred in the Arctic from January to April 2016, but the roles of various physical processes in this period have not been quantified. Here, we utilize an updated version of the coupled atmosphere‒surface climate feedback response analysis method to quantitatively attribute the extreme warmth. …”
Get full text

The Great Lakes Winter Grab: Limnological data from a multi‐institutional winter sampling campaign on the Laurentian Great Lakes by Ge Pu, Kirill Shchapov, Nolan J. T. Pearce, Kelly Bowen, Andrew Bramburger, Andrew Camilleri, Hunter Carrick, Justin D. Chaffin, William Cody, Maureen L. Coleman, Warren J. S. Currie, David C. Depew, Jonathan P. Doubek, Rachel Eveleth, Mark Fitzpatrick, Paul W. Glyshaw, Casey M. Godwin, R. Michael McKay, Mohiuddin Munawar, Heather Niblock, Maci Quintanilla, Michael Rennie, Matthew W. Sand, Kimberly J. Schraitle, Michael R. Twiss, Donald G. Uzarski, Henry A. Vanderploeg, Trista J. Vick‐Majors, Judy A. Westrick, Bridget A. Wheelock, Marguerite A. Xenopoulos, Arthur Zastepa, Ted Ozersky

“…Addressing the winter data gap will enhance our understanding of winter ecosystem function and of lake response to environmental change. Here, we describe a dataset generated by a multi‐institutional winter sampling campaign across all five Laurentian Great Lakes and some of their connecting waters (the Great Lakes Winter Grab). …”
Get full text

Sublimed C60 for efficient and repeatable perovskite-based solar cells by Ahmed A. Said, Erkan Aydin, Esma Ugur, Zhaojian Xu, Caner Deger, Badri Vishal, Aleš Vlk, Pia Dally, Bumin K. Yildirim, Randi Azmi, Jiang Liu, Edward A. Jackson, Holly M. Johnson, Manting Gui, Henning Richter, Anil R. Pininti, Helen Bristow, Maxime Babics, Arsalan Razzaq, Suman Mandal, Thomas G. Allen, Thomas D. Anthopoulos, Martin Ledinský, Ilhan Yavuz, Barry P. Rand, Stefaan De Wolf

“…As perovskite photovoltaic technologies are moving toward industrialization, batch-to-batch reproducibility of device performances becomes crucial. Here, we show that commercial as-received (99.75% pure) C60 source materials may coalesce during repeated thermal evaporation processes, jeopardizing such reproducibility. …”
Get full text