Inhibition of METTL14 overcomes CDK4/6 inhibitor resistance driven by METTL14-m6A-E2F1-axis in ERα-positive breast cancer by Chenlin Liu, Dong Fan, Jiahui Sun, Guodong Li, Ruoxin Du, Xiaoshuang Zuo, Kuo Zhang, Wangqian Zhang, Shuning Wang, Xiaojv Li, Mingrui Du, Donghui Wang, Qiang Hao, Yingqi Zhang, Meng Li, Cun Zhang, Yuan Gao

“…However, CDK4/6i resistance often develops and remains a major hurdle, and the underlying mechanisms remain challenging to fully investigate. Here, we used Genome-wide CRISPR/Cas9 library screening combined with single-cell sequencing to screen for molecules mediating CDK4/6i resistance and identified METTL14 as a determinant of CDK4/6i sensitivity. …”
Get full text

The transcriptional analysis of pepper shed light on a proviral role of light-harvesting chlorophyll a/b binding protein 13 during infection of pepper mild mottle virus by Weihong Lin, Shugen Zhang, Hao Zhang, Xiaomei Deng, Tong Jiang, Xifeng Chen, Laihua Dong, Qin Yan, Lianyi Zang, Yongping Xing, Zhenquan Wang, Qin Zhang, Kaitong Du, Huolin Shen, Junmin Zhang, Tao Zhou

“…Despite its impact, the pathogenicity mechanisms of PMMoV and the pepper plant’s response to infection remain poorly understood. Here, we compared the transcriptomic changes in a susceptible pepper inbred line 21C241 with a resistant inbred line 21C385 seedlings, following systemic PMMoV infection using RNA sequencing. …”
Get full text

IFN-γ reprograms cardiac microvascular endothelial cells to mediate doxorubicin transport and influences the sensitivity of mice to doxorubicin-induced cardiotoxicity by Haoyu Ji, Wenya Ma, Xu Liu, Hongyang Chen, Yining Liu, Zhongyu Ren, Daohong Yin, Ao Cai, Zizhen Zhang, Xin Wang, Wei Huang, Leping Shi, Yanan Tian, Yang Yu, Xiuxiu Wang, Yang Li, Yu Liu, Benzhi Cai

“…The sensitivity to DIC varies among patients, but the precise mechanisms remain elusive. Here we constructed a mouse model of DIC using DOX to investigate potential mechanisms contributing to the differential susceptibility to DIC. …”
Get full text

The cryptic lncRNA-encoded microprotein TPM3P9 drives oncogenic RNA splicing and tumorigenesis by Kun Meng, Yuying Li, Xiaoyi Yuan, Hui-Min Shen, Li-Ling Hu, Danya Liu, Fujin Shi, Dandan Zheng, Xinyu Shi, Nengqiao Wen, Yun Cao, Yun-Long Pan, Qing-Yu He, Chris Zhiyi Zhang

“…However, the importance and underlying mechanisms of these microproteins in alternative splicing-driven tumor progression have rarely been studied. Here, we show that the novel protein TPM3P9, encoded by the lncRNA tropomyosin 3 pseudogene 9, exhibits oncogenic activity in clear cell renal cell carcinoma (ccRCC) by enhancing oncogenic RNA splicing. …”
Get full text

Spectrum and Location of Ongoing Extreme Particle Acceleration in Cassiopeia A by Jooyun Woo, Kaya Mori, Charles J. Hailey, Elizabeth Spira-Savett, Aya Bamba, Brian W. Grefenstette, Thomas B. Humensky, Reshmi Mukherjee, Samar Safi-Harb, Tea Temim, Naomi Tsuji

“…Nevertheless, the precise location, duration, and operation of CR acceleration in young SNRs are open questions. Here, we report on multiepoch X-ray observations of Cassiopeia A (Cas A), a 350 yr old SNR, in the 15–50 keV band that probes the most energetic CR electrons. …”
Get full text

Similarity or complementarity? Understanding marital relationships in terms of sexual dimorphism in brain morphometry and gender roles by Lei Li, Xinyue Huang, Qingyu Zheng, Jinming Xiao, Xiaolong Shan, Huafu Chen, Xujun Duan

“…Sex differences provide a theoretical integration of these two conflicting statements. Here, we explored the relationship between marital satisfaction and sex differences in social attributes and neuroanatomical characteristics in 48 married couples. …”
Get full text

Transcriptome analysis reveals hypoxic response key genes and modules as well as adaptive mechanism of crucian carp (Carassius auratus) gill under hypoxic stress by Mengchao Xing, Mengchao Xing, Zhen Rong, Zhen Rong, Xin Zhao, Xin Zhao, Xiaowei Gao, Xiaowei Gao, Zhiguang Hou, Lihan Zhang, Lihan Zhang, Waiho Khor, Yihuan Xu, Yihuan Xu, Li Chen, Li Chen, Chengbin Wu, Chengbin Wu

“…However, the adaptive mechanism of crucian carp to hypoxic stress remains largely unknown. Here, we investigated gill physiological and transcriptomic changes of crucian carp exposed to hypoxic conditions (dissolved oxygen concentration of 0.6 ± 0.3 mg/L) for different durations (0 d, 1 d, 2d, 3d, 4 d, and 5d). …”
Get full text

Country-Specific Roadmaps for Scaling Up Integrated Care in Belgium, Slovenia, and Cambodia – Lessons Learned from the SCUBY Project by Martin Heine, Monika Martens, Daniel Boateng, Grace Marie Ku, Roy Remmen, Edwin Wouters, Srean Chhim, Por Ir, Antonjia Poplas Susič, Wim van Damme, Josefien van Olmen, Kerstin Klipstein-Grobusch, on behalf of the SCUBY consortium

“…Introduction: The SCUBY project aimed to provide knowledge on the scaling-up of an Integrated Care Package (ICP) for type 2 diabetes and hypertension across three distinct health systems (Cambodia, Slovenia, and Belgium). Here, we analyse the different elements of the country-specific scale-up roadmaps to identify similarities and differences, and share lessons learned. …”
Get full text

Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo by Xiangwei Liu, Naiwen Tan, Yuchao Zhou, Xueying Zhou, Hui Chen, Hongbo Wei, Ji Chen, Xiaoru Xu, Sijia Zhang, Guodong Yang, Yingliang Song

“…Taken together, our study here identifies Sema3A as a critical gene for osteogenic phenotype and reveals that Sema3A-modified ASCs would serve as a promising candidate for bettering bone defect repair.…”
Get full text

Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site by Maria Paula Roberti, Pornpimol Charoentong, Yanhong Lyu, Marten Meyer, Stefan B. Eichmüller, Patrick Schmidt, Frank Momburg, Miray Cetin, Felix Hartmann, Nektarios A. Valous, Albrecht Stenzinger, Laura Michel, Peter Lichter, Andreas Schneeweiss, Verena Thewes, Carlo Fremd, Inka Zörnig, Dirk Jäger

“…Therefore, novel approaches to isolate tumor-specific T cells are needed. Here, we report the isolation of neoantigen-specific CD8+ T cells from a vaccination site of a metastatic breast cancer patient who received a personalized vaccine. …”
Get full text

A structure-based designed small molecule depletes hRpn13Pru and a select group of KEN box proteins by Xiuxiu Lu, Monika Chandravanshi, Venkata R. Sabbasani, Snehal Gaikwad, V. Keith Hughitt, Nana Gyabaah-Kessie, Bradley T. Scroggins, Sudipto Das, Wazo Myint, Michelle E. Clapp, Charles D. Schwieters, Marzena A. Dyba, Derek L. Bolhuis, Janusz W. Koscielniak, Thorkell Andresson, Michael J. Emanuele, Nicholas G. Brown, Hiroshi Matsuo, Raj Chari, Deborah E. Citrin, Beverly A. Mock, Rolf E. Swenson, Kylie J. Walters

“…A KEN box, but not ubiquitination, is required for XL44-induced depletion of PCLAF. Here, we show that XL44 induces ubiquitin-dependent loss of hRpn13Pru and ubiquitin-independent loss of select KEN box containing proteins.…”
Get full text

Chronic Stress‐Induced and Tumor Derived SP1+ Exosomes Polarizing IL‐1β+ Neutrophils to Increase Lung Metastasis of Breast Cancer by Leyi Zhang, Jun Pan, Meijun Wang, Jini Yang, Sangsang Zhu, Lili Li, Xiaoxiao Hu, Zhen Wang, Liwei Pang, Peng Li, Fang Jia, Guohong Ren, Yi Zhang, Danying Xu, Fuming Qiu, Jian Huang

“…However, whether tumor‐derived exosomes (TDEs) are influenced by chronic stress and participate in chronic stress‐mediated distant metastasis remains unclear. Here, it is shown that chronic stress remarkably facilitates the secretion of TDEs and modifies the contents of exosomes by activating the adrenergic β receptor in 4T1 tumor‐bearing mice. …”
Get full text

Oncogenic role of RARG rearrangements in acute myeloid leukemia resembling acute promyelocytic leukemia by Feng Wang, Luyao Zhao, Yun Tan, Xufeng Cen, Huan Gao, Huimin Jiang, Ying Liu, Yunxuan Li, Tingting Zhang, Chenxi Zhao, Ting Shi, Guilin Xu, Churan Wang, Jiong Hu, Xia Li, Ya-Zhen Qin, Kankan Wang, Hong-Hu Zhu, Ke Li

“…However, the mechanisms underlying the role of RARG fusions in leukemogenesis remain elusive. Here, we show that RARG fusions disrupt myeloid differentiation and promote proliferation and self-renewal of hematopoietic stem and progenitor cells (HSPCs) by upregulating BCL2 and ATF3. …”
Get full text

Ganoboninketal C from Ganoderma boninense improves the efficacy of CDDP-based chemotherapy through inhibiting translesion DNA synthesis by Xiaolu Ma, Fei Yang, Ke Ma, Hongyan Shen, Junjie Han, Kai Wang, Yeran Yang, Jiawei Zhu, Ruiyuan An, Qilin Wang, Tie-Shan Tang, Bo Zhou, Hongwei Liu, Caixia Guo

“…Unfortunately, few suitable natural TLS inhibitors have been reported. Here, we found that a triterpenoid compound Ganoboninketal C (26-3) from Ganoderma boninense, a traditional Chinese medicine, can impair CDDP-induced TLS polymerase eta (Polη) focus formation, PCNA monoubiquitination as well as mutagenesis. …”
Get full text

Regarder au-delà des élections : l’historicité complexe de la participation politique dans la Libye contemporaine by Chiara Loschi, Chiara Pagano

“…To shed light on the current political scenario, we propose here to reconsider the political from below, and namely by looking where only disruptions to the neo-liberal model of functioning democracies have been detected so far - and thus in most manifestations referred to as 'chaos', 'statelessness' or absence of 'political culture'. …”
Get full text

The spatial landscape of cancer hallmarks reveals patterns of tumor ecological dynamics and drug sensitivity by Mustafa Sibai, Sergi Cervilla, Daniela Grases, Eva Musulen, Rossana Lazcano, Chia-Kuei Mo, Veronica Davalos, Arola Fortian, Adrià Bernat, Margarita Romeo, Collin Tokheim, Jordi Barretina, Alexander J. Lazar, Li Ding, Enrique Grande, Francisco X. Real, Manel Esteller, Matthew H. Bailey, Eduard Porta-Pardo, Enrique Grande, Teresa Alonso-Gordoa, Mario Álvarez-Maestro, Elena Andrada, Ainara Azueta, Raquel Benítez Javier Burgos, Daniel Castellano, M. Angel Climent, Mario Domínguez, Ignacio Durán Albert Font, Isabel Galante, Patricia Galván, Juan F. García, Xavier García del Muro, Félix Guerrero-Ramos, Núria Malats, Miriam Marqués, Pablo Maroto, Jaime Martínez de Villarreal, Ane Moreno-Oya, Jesús M. Paramio, Alvaro Pinto, Aleix Prat, Javier Puente, Oscar Reig, Francisco X. Real

“…The concept of cancer hallmarks distills this complexity into underlying principles that govern tumor growth. Here, we explore the spatial distribution of cancer hallmarks across 63 primary untreated tumors from 10 cancer types using spatial transcriptomics. …”
Get full text

Genomic characterization of three marine fungi, including Emericellopsis atlantica sp. nov. with signatures of a generalist lifestyle and marine biomass degradation by Ole Christian Hagestad, Lingwei Hou, Jeanette H. Andersen, Espen H. Hansen, Bjørn Altermark, Chun Li, Eric Kuhnert, Russell J. Cox, Pedro W. Crous, Joseph W. Spatafora, Kathleen Lail, Mojgan Amirebrahimi, Anna Lipzen, Jasmyn Pangilinan, William Andreopoulos, Richard D. Hayes, Vivian Ng, Igor V. Grigoriev, Stephen A. Jackson, Thomas D. S. Sutton, Alan D. W. Dobson, Teppo Rämä

“…A six-gene phylogenetic analysis placed the isolate in the marine Emericellopsis clade and morphological examination confirmed that the isolate represents a new species, which is described here as E. atlantica. Analysis of its CAZyme repertoire and a culturing experiment on three marine and one terrestrial substrates indicated that E. atlantica is a psychrotrophic generalist fungus that is able to degrade several types of marine biomass. …”
Get full text

Enabling next-generation engineered TCR-T therapies based on high-throughput TCR discovery from diagnostic tumor biopsies by Thomas Kuilman, Deborah S. Schrikkema, Jules Gadiot, Raquel Gomez-Eerland, Laura Bies, Julia Walker, Robbert M. Spaapen, Hanna Kok, Demi Houg, Milena Viyacheva, Yvonne B. Claassen, Manuel Saornil, Oscar Krijgsman, Bas Stringer, Huiwen Ding, Anou Geleijnse, Anne C. Meinema, Bianca Weissbrich, Melissa Lancee, Carmen G. Engele, Marianna Sabatino, Pei-Ling Chen, Kenneth Y. Tsai, James J. Mulé, Vernon K. Sondak, Jitske van den Bulk, Noel F. de Miranda, Inge Jedema, John G. Haanen, Jeroen W. J. van Heijst, Ton N. Schumacher, Carsten Linnemann, Gavin M. Bendle

“…However, its wider application and efficacy has been restricted by the limited accessibility, proliferative capacity and effector function of tumor-specific TIL. Here, we develop a platform for the efficient identification of tumor-specific TCR genes from diagnostic tumor biopsies, including core-needle biopsies frozen in a non-viable format, to enable engineered T cell therapy. …”
Get full text

Enhancing thermoelectric properties of ScN films through twin domains by J. More-Chevalier, U.D. Wdowik, J. Martan, T. Baba, S. Cichoň, P. Levinský, D. Legut, E. de Prado, P. Hruška, J. Pokorný, J. Bulíř, C. Beltrami, T. Mori, M. Novotný, I. Gregora, L. Fekete, L. Volfová, J. Lančok

“…Tailoring thermoelectric properties of ScN-based materials is of vital importance for their application, particularly at high operating temperatures. Here, we report on the thermoelectric properties of the ScN layers deposited on MgO (001) substrates by the DC reactive magnetron sputtering. …”
Get full text

Extracellular vesicles, RNA sequencing, and bioinformatic analyses: Challenges, solutions, and recommendations by Rebecca T. Miceli, Tzu‐Yi Chen, Yohei Nose, Swapnil Tichkule, Briana Brown, John F. Fullard, Marilyn D. Saulsbury, Simon O. Heyliger, Sacha Gnjatic, Natasha Kyprianou, Carlos Cordon‐Cardo, Susmita Sahoo, Emanuela Taioli, Panos Roussos, Gustavo Stolovitzky, Edgar Gonzalez‐Kozlova, Navneet Dogra

“…Consequently, there is a gap in knowledge and the need for a standardized approach in delineating EV‐RNAs. Here, we address these gaps by describing the following points by (1) focusing on the large canopy of the EVs and particles (EVPs), which includes, but not limited to – exosomes and other large and small EVs, lipoproteins, exomeres/supermeres, mitochondrial‐derived vesicles, RNA binding proteins, and cell‐free DNA/RNA/proteins; (2) examining the potential functional roles and biogenesis of EVPs; (3) discussing various transcriptomic methods and technologies used in uncovering the cargoes of EVPs; (4) presenting a comprehensive list of RNA subtypes reported in EVPs; (5) describing different EV‐RNA databases and resources specific to EV‐RNA species; (6) reviewing established bioinformatics pipelines and novel strategies for reproducible EV transcriptomics analyses; (7) emphasizing the significant need for a gold standard approach in identifying EV‐RNAs across studies; (8) and finally, we highlight current challenges, discuss possible solutions, and present recommendations for robust and reproducible analyses of EVP‐associated small RNAs. …”
Get full text