Optimization of chemical transfection in airway epithelial cell lines by Tony J. F. Guo, Wan Yi Liang, Gurpreet K. Singhera, Jasmine Memar Vaghri, Janice M. Leung, Del R. Dorscheid

“…Additionally, it has been explored for its potential application in delivering gene therapies. Here, we characterize the transfection efficiency of EX-EGFP-Lv105, an EGFP-expressing plasmid into three cell lines commonly used to model the airway epithelium (1HAEo-, 16HBE14o-, and NCI-H292). …”
Get full text

Functional Analysis of Durum Wheat GASA1 Protein as a Biotechnological Alternative Against Plant Fungal Pathogens and a Positive Regulator of Biotic Stress Defense by Mohamed Taieb Bouteraa, Walid Ben Romdhane, Alina Wiszniewska, Narjes Baazaoui, Mohammad Y. Alfaifi, Anis Ben Hsouna, Miroslava Kačániová, Stefania Garzoli, Rania Ben Saad

“…Snakins are such compounds, encoded by the <i>GASA</i> (Gibberellic Acid-Stimulated Arabidopsis) gene family, and are involved in the response to biotic and abiotic stress. Here, we examined the function of the newly identified TdGASA1 gene and its encoded protein in <i>Triticum durum</i> subjected to different biotic stress-related simulants, such as mechanical injury, methyl jasmonate (MeJA), indole-3-acetic acid (IAA), salicylic acid (SA), hydrogen peroxide (H₂O₂), as well as infection with pathogenic fungi <i>Fusarium graminearum</i> and <i>Aspergillus niger</i>. …”
Get full text

Shifts in the rhizosphere microbiome and exudation profile of avocado (Persea americana Mill.) during infection by Phytophthora cinnamomi and in presence of a biocontrol bacterial... by Frédérique Reverchon, Mariel García-Meléndez, Edgar Guevara-Avendaño, Oscar Mora-Chávez, Itzel A. Solís-García, Wesley Dáttilo, José A. Guererro-Analco, Alfonso Méndez-Bravo, Juan L. Monribot-Villanueva, Violeta Patiño-Conde, Fernando Pineda-García, Eria A. Rebollar

“…We also evaluated the effect of inoculating a bacterial biological control agent (BCA) of P. cinnamomi on the avocado rhizosphere microbiome, in the presence and absence of the pathogen, and on morphological and physiological plant variables, to confirm the potential of the BCA to alleviate the stress induced by the disease. Dataset presentation Here, we present a novel dataset collected from a time-course experiment with four treatments: (1) control trees; (2) trees infected with P. cinnamomi; (3) trees inoculated with the BCA; (4) trees infected with P. cinnamomi and inoculated with the BCA. …”
Get full text

Genomic epidemiology and antimicrobial resistance of Morganella clinical isolates between 2016 and 2023 by Wentao Zhu, Qian Liu, Jinlv Liu, Jinlv Liu, Yaqi Wang, Yaqi Wang, Hong Shen, Ming Wei, Ji Pu, Li Gu, Jing Yang, Jing Yang, Jing Yang

“…Morganella morganii is a Gram-negative, opportunistic pathogen that is often associated with nosocomial infections. Here, the genomic characteristics and antimicrobial resistance (AMR) of Morganella clinical isolates between 2016 and 2023 were determined. …”
Get full text

Comparative transcriptomic rhythms in the mouse and human prefrontal cortex by Jennifer N. Burns, Jennifer N. Burns, Aaron K. Jenkins, Xiangning Xue, Kaitlyn A. Petersen, Kaitlyn A. Petersen, Kyle D. Ketchesin, Kyle D. Ketchesin, Megan S. Perez, Megan S. Perez, Chelsea A. Vadnie, Madeline R. Scott, Madeline R. Scott, Marianne L. Seney, Marianne L. Seney, George C. Tseng, Colleen A. McClung, Colleen A. McClung

“…However, investigation into the potential circadian mechanisms underlying these diseases in animal models must contend with the fact that the human PFC is highly evolved and specialized relative to that of rodents.MethodsHere, we use RNA sequencing to lay the groundwork for translational studies of molecular rhythms through a sex-specific, cross species comparison of transcriptomic rhythms between the mouse medial PFC (mPFC) and two subregions of the human PFC, the anterior cingulate cortex (ACC) and the dorsolateral PFC (DLPFC).ResultsWe find that while circadian rhythm signaling is conserved across species and subregions, there is a phase shift in the expression of core clock genes between the mouse mPFC and human PFC subregions that differs by sex. …”
Get full text

Unveiling the charge density wave mechanism in vanadium-based Bi-layered kagome metals by Yi-Chen Yang, Soohyun Cho, Tong-Rui Li, Xiang-Qi Liu, Zheng-Tai Liu, Zhi-Cheng Jiang, Jian-Yang Ding, Wei Xia, Zi-Cheng Tao, Jia-Yu Liu, Wen-Chuan Jing, Yu Huang, Yu-Ming Shi, Soonsang Huh, Takeshi Kondo, Zhe Sun, Ji-Shan Liu, Mao Ye, Yi-Lin Wang, Yan-Feng Guo, Da-Wei Shen

“…Recently, the vanadium-based bi-layered kagome metal ScV6Sn6, reported to exhibit a long-range charge order below 94 K, has emerged as a promising candidate to further clarify this core issue. Here, employing micro-focusing angle-resolved photoemission spectroscopy (μ-ARPES) and first-principles calculations, we systematically studied the unique CDW order in vanadium-based bi-layered kagome metals by comparing ScV6Sn6 with its isostructural counterpart YV6Sn6, which lacks a CDW ground state. …”
Get full text

Suppressing recurrence in Sonic Hedgehog subgroup medulloblastoma using the OLIG2 inhibitor CT-179 by Yuchen Li, Chaemin Lim, Taylor Dismuke, Daniel S. Malawsky, Sho Oasa, Zara C. Bruce, Carolin Offenhäuser, Ulrich Baumgartner, Rochelle C. J. D’Souza, Stacey L. Edwards, Juliet D. French, Lucy S. H. Ock, Sneha Nair, Haran Sivakumaran, Lachlan Harris, Andrey P. Tikunov, Duhyeong Hwang, Coral Del Mar Alicea Pauneto, Mellissa Maybury, Timothy Hassall, Brandon Wainwright, Santosh Kesari, Gregory Stein, Michael Piper, Terrance G. Johns, Marina Sokolsky-Papkov, Lars Terenius, Vladana Vukojević, Leon F. McSwain, Timothy R. Gershon, Bryan W. Day

“…Abstract OLIG2-expressing tumor stem cells have been shown to drive recurrence in Sonic Hedgehog (SHH)-subgroup medulloblastoma (MB) and patients urgently need specific therapies to target this tumor cell population. Here, we investigate the therapeutic potential of the brain-penetrant orally bioavailable, OLIG2 inhibitor CT-179, using SHH-MB explant organoids, PDX and GEM SHH-MB models. …”
Get full text

Standardization, validation, and comparative evaluation of a convenient surrogate recombinant vesicular stomatitis virus plaque reduction test for quantification of Hantaan orthoha... by Jing Wei, Hui Zhang, Jiawei Pei, Qiqi Yang, Yuan Wang, Xiaolei Jin, He Liu, Liang Zhang, Hongwei Ma, Linfeng Cheng, Yangchao Dong, Yingfeng Lei, Yinlan Bai, Zhikai Xu, Pengbo Yu, Fanglin Zhang, Wei Ye

“…The golden standard for HTNV NAbs measurement is the focus reduction neutralization test (FRNT), which typically requires skilled technicians and is performed under high biosafety containment facility. Here, we established a surrogate NAbs titration method with replication-competent vesicular stomatitis virus (VSV) bearing HTNV glycoprotein (rVSV-HTNV-GP) based plaque reduction neutralization test (PRNT). …”
Get full text

Genome-wide association study unravels mechanisms of brain glymphatic activity by Shu-Yi Huang, Yi-Jun Ge, Peng Ren, Bang-Sheng Wu, Weikang Gong, Jing Du, Shi-Dong Chen, Ju-Jiao Kang, Qing Ma, Arun L. W. Bokde, Sylvane Desrivières, Hugh Garavan, Antoine Grigis, Herve Lemaitre, Michael N. Smolka, Sarah Hohmann, IMAGEN Consortium, Jian-Feng Feng, Ya-Ru Zhang, Wei Cheng, Jin-Tai Yu

“…Abstract Brain glymphatic activity, as indicated by diffusion-tensor imaging analysis along the perivascular space (ALPS) index, is involved in developmental neuropsychiatric and neurodegenerative diseases, but its genetic architecture is poorly understood. Here, we identified 17 unique genome-wide significant loci and 161 candidate genes linked to the ALPS-indexes in a discovery sample of 31,021 individuals from the UK Biobank. …”
Get full text

Epidemiology of bacterial biofilms on polyps and normal tissues in a screening colonoscopy cohort by Julia L. Drewes, Samara B. Rifkin, Madison McMann, Sara Glass, Emma Spence, Caroline R. Wensel, Abby L. Geis, Courtney Stevens, Joell J. Gills, Hao Wang, Linda M. Hylind, Gerard Mullin, David Kafonek, David Cromwell, Louis La Luna, Francis M. Giardiello, Cynthia L. Sears

“…However, their prevalence on histologically normal tissues and polyps is not well established, and risk factors of biofilms have not been previously investigated. Here we evaluated potential procedural and demographic risk factors associated with biofilm status using a cross-sectional observational cohort.Methods Histologically normal colonic biopsies from 2,051 individuals undergoing screening colonoscopy were evaluated for biofilm status using fluorescence in situ hybridization with oligonucleotide probes targeting bacterial 16S rRNA. …”
Get full text

Group a Streptococcus remains viable inside fibrin clots and gains access to human plasminogen for subsequent fibrinolysis and dissemination by Henry M. Vu, Thomas E. Moran, Zhong Liang, Yun-Juan Bao, Paulina G. Carles, Jessica C. Keane, Madelyn G. Cerney, Caitlyn N. Dahnke, Ana L. Flores-Mireles, Victoria A. Ploplis, Francis J. Castellino, Shaun W. Lee

“…A major unresolved question regards the temporal dynamics of how GAS enmeshed in a fibrin clot can access plasminogen for clot dissolution and eventual dissemination. Here, we reveal through live imaging studies that GAS trapped inside a fibrin clot can remain viable in a latent state, until access to plasminogen activates fibrinolysis and dissemination. …”
Get full text

A low-protein soybean-free diet improves carcass traits and meat quality and modulates the colonic microbiota in Daweizi pigs by Haohua Fu, Taoming Yang, Hengjia Ni, Hengjia Ni, Jing Li, Fenfen Liu, Jingbo Liu, Yulong Yin, Yulong Yin

“…The use of safe and functional unconventional feed sources can effectively alleviate worldwide protein shortage problems.MethodsHere, we formulated a low-protein soybean-free diet (LPNS) for growing and fattening pigs using rice, potatoes, tea, and other unconventional feed sources. …”
Get full text

Sex differences in the relationships between 24-h rest-activity patterns and plasma markers of Alzheimer’s disease pathology by Maxime Van Egroo, Elise Beckers, Nicholas J. Ashton, Kaj Blennow, Henrik Zetterberg, Heidi I. L. Jacobs

“…Abstract Background Although separate lines of research indicated a moderating role of sex in both sleep-wake disruption and in the interindividual vulnerability to Alzheimer’s disease (AD)-related processes, the quantification of sex differences in the interplay between sleep-wake dysregulation and AD pathology remains critically overlooked. Here, we examined sex-specific associations between circadian rest-activity patterns and AD-related pathophysiological processes across the adult lifespan. …”
Get full text

Metabolic and molecular Characterization, following dietary exposure to DINCH, Reveals new Implications for its role as a Metabolism-Disrupting chemical by Sontje Krupka, Alix Sarah Aldehoff, Cornelius Goerdeler, Beatrice Engelmann, Ulrike Rolle-Kampczyk, Kristin Schubert, Nora Klöting, Martin von Bergen, Matthias Blüher

“…However, metabolic and molecular effects are not fully understood in vivo.Here, we performed a comprehensive in vivo analysis using C57BL/6N mice to investigate the effects of DINCH on adipose tissue physiology and function. …”
Get full text

Whole-genome resequencing landscape of adaptive evolution in Relict gull (Larus relictus) by Chao Yang, Qingxiong Wang, Kuo Sun, Lei Luo, Hao Yuan, Xuejuan Li, Yuan Huang, Hong Xiao

“…However, our knowledge of the evolutionary history of L. relictus is limited. Here, we performed whole-genome resequencing of L. relictus (n = 14) and L. brunnicephalus (n = 3) to explore the genetic relationships and population structures and understand their adaptive evolution. …”
Get full text

Real-world of Limosilactobacillus reuteri in mitigation of acute experimental colitis by Ningning Yue, Hailan Zhao, Peng Hu, Yuan Zhang, Chengmei Tian, Chen Kong, Zhiliang Mai, Longbin Huang, Qianjun Luo, Daoru Wei, Ruiyue Shi, Shaohui Tang, Yuqiang Nie, Yujie Liang, Jun Yao, Lisheng Wang, Defeng Li

“…However, the underlying mechanisms mediating microbiota-host crosstalk remain largely elusive. Here, we report that Limosilactobacillus reuteri (L. reuteri), as a probiotic, secretes cytoplasmic membrane vesicles (CMVs) that communicate with host cells, alter host physiology, and alleviate dextran sulfate sodium (DSS)-induced colitis. …”
Get full text

Field‐Induced Antiferromagnetic Correlations in a Nanopatterned Van der Waals Ferromagnet: A Potential Artificial Spin Ice by Avia Noah, Nofar Fridman, Yishay Zur, Maya Markman, Yotam Katz King, Maya Klang, Ricardo Rama‐Eiroa, Harshvardhan Solanki, Michael L. Reichenberg Ashby, Tamar Levin, Edwin Herrera, Martin E. Huber, Snir Gazit, Elton J. G. Santos, Hermann Suderow, Hadar Steinberg, Oded Millo, Yonathan Anahory

“…With the advent of atomically thin 2D van der Waals (vdW) magnets, a pertinent question is whether such compounds could make their way into this realm where interactions can be tailored so that unconventional states of matter can be assessed. Here, it is shown that square islands of CrGeTe3 vdW ferromagnets distributed in a grid manifest antiferromagnetic correlations, essential to enable frustration resulting in an artificial spin‐ice. …”
Get full text

Identification and functional characterization of AsWRKY9, a WRKY transcription factor modulating alliin biosynthesis in garlic (Allium sativum L.) by Jiaying Wu, Min Li, Wanni Wang, Yiren Su, Jie Li, Jiaxin Gong, Xianfeng Meng, Chenyuan Lin, Qiantong Zhang, Yanyan Yang, Chunyan Xu, Limei Zeng, Jihong Jiang, Xuqin Yang

“…However, the molecular mechanisms regulating alliin biosynthesis remain unexplored. Here, we report for the first time that a WRKY family transcription factor regulates the expression of a key enzyme gene in the alliin biosynthesis pathway, enhancing the accumulation of alliin. …”
Get full text

Chronic Rhinosinusitis with Nasal Polyps and Biologics: A Call for Better Data Standardisation and Presentation in Clinical Studies by Kariyawasam HH, Langan D, Rimmer J

“…Such RCTs must facilitate meta-analysis. Here we report the need for urgent improvement in double-blind randomised controlled trials of biologics in CRSwNP, having previously undertaken a systematic review and meta-analysis of such studies. …”
Get full text

Host-Induced Gene Silencing of the <i>Aspergillus flavus O</i>-Methyl Transferase Gene Enhanced Maize Aflatoxin Resistance by Olanike Omolehin, Yenjit Raruang, Dongfang Hu, Zhu-Qiang Han, Surassawadee Promyou, Robert L. Brown, Qijian Wei, Kanniah Rajasekaran, Jeffrey W. Cary, Kan Wang, Dan Jeffers, Zhi-Yuan Chen

“…Maize is one of the major crops that are susceptible to <i>Aspergillus flavus</i> infection and subsequent aflatoxin contamination, which poses a serious health threat to humans and domestic animals. Here, an RNA interference (RNAi) approach called Host-Induced Gene Silencing (HIGS) was employed to suppress the <i>O</i>-methyl transferase gene (<i>omtA</i>, also called <i>aflP</i>), a key gene involved in aflatoxin biosynthesis. …”
Get full text