A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells by Anna Babin-Ebell Gonçalves, Yifang Mao, Tinja Baljkas, Felix Wiedmann, Larissa Eis, Franziska Pilz, Manuel Winkler, Sina W. Kürschner-Zacharias, Marlene Hoffarth, Charlotta Funaya, Réza Shahidi, Cyrill Géraud, Chi-Chung Wu, Constanze Schmidt, Sergij Goerdt, Philipp-Sebastian Reiners-Koch, Mahak Singhal

“…Current techniques to isolate LSECs, relying on perfusion-dependent liver digestion, are cumbersome with limited throughput. We here describe a perfusion-independent high-throughput procedure to isolate LSECs with high purity. …”
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An EED/PRC2‐H19 Loop Regulates Cerebellar Development by Pei‐Pei Liu, Xiao Han, Xiao Li, Shang‐Kun Dai, Ya‐Jie Xu, Lin‐Fei Jiao, Hong‐Zhen Du, Li‐Hua Zhao, Rong‐Feng Li, Zhao‐Qian Teng, Yun‐Gui Yang, Chang‐Mei Liu

“…However, its biological function in cerebellar development remains unknown. Here, we show that EED deletion from neural stem cells (NSCs) or cerebellar granule cell progenitors (GCPs) leads to reduced GCPs proliferation, cell death, cerebellar hypoplasia, and motor deficits in mice. …”
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The zinc receptor, ZnR/GPR39, modulates taste sensitivity by regulating ion secretion in mouse salivary gland by Moran Melamed, Hila Asraf, Noa Livne, Milos Bogdanovic, Anil Shendge, Gilad Shamir, Maayan Mero, Omer Adir, Avi Schroeder, Israel Sekler, Michal Hershfinkel

“…A mechanism for zinc regulation of these processes is lacking. Here, we identified the Zn2+ sensing receptor ZnR/GPR39 as a mediator of ion transport in salivary gland epithelium. …”
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A Systemic Approach to Inceptive Constructions in Lithuanian by Rolandas Mikulskas

“…The remaining verbs in the list mostly presuppose agentive, intentional referents as their subjects. Exceptions here are the verbs prapliupo, pratrūko and pasiuto, pašėlo which designate the beginning of spontaneous, uncontrolled events. …”
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A charge reversal nano-assembly prevents hepatic steatosis by resolving inflammation and improving lipid metabolism by Haoyu Wang, Sheng'e Su, Xin An, Yuan Xu, Jiacheng Sun, Mingming Zhen, Chunru Wang, Chunli Bai

“…However, on-demand anchoring inflammation and lipid metabolism disorder for hepatic stestosis treatment has yet to be realized. Here we propose a charge reversal fullerene based nano-assembly to migrate hepatic steatosis via inhibiting macrophage-mediated inflammation and normalizing hepatocellular lipid metabolism in obesity mice. …”
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Construction of a Vero cell line expression human KREMEN1 for the development of CVA6 vaccines by Dongqing Zhang, Yuxiang Zou, Jiaying Wu, Longfa Xu, Zhifeng Ke, Yuanyuan Wu, Zhenhong Zhou, Mujin Fang, Ling Chen, Henggang Xu, Jianping Chu, Ningshao Xia, Rui Zhu, Tong Cheng

“…The expression level of virus-specific receptors on the cell membrane significantly influences viral infection. Here, a Vero cell line with stable over-expressing of KREMEN1 (KRM1), a crucial receptor for CVA6, was constructed using the lentivirus system. …”
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Source-specific acute cardio-respiratory effects of ambient coarse particulate matter exposure in California’s Salton Sea region by Yaning Miao, William C Porter, Tarik Benmarhnia, Catherine Lowe, Timothy W Lyons, Caroline Hung, Charles Diamond

“…However, these potential effects remain understudied. Here we use observed coarse particulate matter (PM _c ) concentrations and modeled atmospheric back trajectories along with land surface data to estimate individual source region contributions to particulates observed at long term surface air quality monitoring sites in the Salton Sea region. …”
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Preliminary effectiveness of social prescription and virtual patient information in increasing tertiary prevention among cancer patients (ESPRIT): protocol for a single-centre, ran... by Karen Steindorf, Michael Koller, Michael Leitzmann, Wolfgang Herr, Daniel Heudobler, Lisa Boyer, Anne Herrmann, Tobias Pukrop, Christine Bernardi, Sabine Einhell

“…However, more research is required on the effectiveness of strategies designed to increase the uptake of tertiary prevention programmes among cancer patients. Here, we present the protocol for a single-centre, randomised controlled pilot trial testing the preliminary effectiveness of social prescription and virtual patient information in increasing tertiary prevention among cancer patients and support persons (SPs) (ESPRIT “Effectiveness of a social prescription and virtual patient information in increasing tertiary prevention” pilot trial).Methods and analysis Cancer patients attending medical oncology units at a university hospital in southern Germany and their SPs will be randomly allocated as a dyad to group A (social prescription (n=36)), group B (virtual patient information (n=36)) or group C (usual care (n=36)). …”
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Unveiling emerging polycyclic aromatic compounds in the urban atmospheric particulate matter by Jia-Yong Lao, Tingyu Li, Le Jing, Xian Qin, Wenyuan Su, Huiju Lin, Yuefei Ruan, Ting Ruan, Eddy Y. Zeng, Kenneth M.Y. Leung, Paul K.S. Lam

“…Additionally, absorption, distribution, metabolism, excretion, and toxicity properties were evaluated to indicate PAC’s overall abilities to cause adverse outcomes and incorporated into a novel health risk assessment model to assess their inhalation risks. Here, except for target PACs, 98 PAC analogues across eight categories were identified in the outdoor samples of atmospheric particulate matter. …”
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Interferon-α promotes HLA-B-restricted presentation of conventional and alternative antigens in human pancreatic β-cells by Alexia Carré, Fatoumata Samassa, Zhicheng Zhou, Javier Perez-Hernandez, Christiana Lekka, Anthony Manganaro, Masaya Oshima, Hanqing Liao, Robert Parker, Annalisa Nicastri, Barbara Brandao, Maikel L. Colli, Decio L. Eizirik, Jahnavi Aluri, Deep Patel, Marcus Göransson, Orlando Burgos Morales, Amanda Anderson, Laurie Landry, Farah Kobaisi, Raphael Scharfmann, Lorella Marselli, Piero Marchetti, Sylvaine You, Maki Nakayama, Sine R. Hadrup, Sally C. Kent, Sarah J. Richardson, Nicola Ternette, Roberto Mallone

“…Abstract Interferon (IFN)-α is the earliest cytokine signature observed in individuals at risk for type 1 diabetes (T1D), but the effect of IFN-α on the antigen repertoire of HLA Class I (HLA-I) in pancreatic β-cells is unknown. Here we characterize the HLA-I antigen presentation in resting and IFN-α-exposed β-cells and find that IFN-α increases HLA-I expression and expands peptide repertoire to those derived from alternative mRNA splicing, protein cis-splicing and post-translational modifications. …”
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NF-κB/AP-1-Targeted Inhibition of Macrophage-Mediated Inflammatory Responses by Depigmenting Compound AP736 Derived from Natural 1,3-Diphenylpropane Skeleton by Van Thai Ha, Heung Soo Beak, Eunji Kim, Kwang-Soo Baek, Muhammad Jahangir Hossen, Woo Seok Yang, Yong Kim, Jun Ho Kim, Sungjae Yang, Jeong-Hwan Kim, Yung Hyup Joo, Chang Seok Lee, Joonho Choi, Hong-Ju Shin, Sungyoul Hong, Song Seok Shin, Jae Youl Cho

“…Since macrophage-mediated inflammatory responses are critical for skin health, here we investigated the potential anti-inflammatory activity of AP736. …”
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RNA-seq reproducibility of Pseudomonas aeruginosa in laboratory models of cystic fibrosis by Rebecca P. Duncan, Gina R. Lewin, Daniel M. Cornforth, Frances L. Diggle, Ananya Kapur, Dina A. Moustafa, Yasmin Hilliam, Jennifer M. Bomberger, Marvin Whiteley, Joanna B. Goldberg

“…We leverage a large data set that includes samples prepared in three different laboratories and paired data sets where the same sample was sequenced using at least two different sequencing pipelines. We report here that expression data are highly reproducible across laboratories. …”
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Liver metastases of colorectal cancer contain different subsets of tissue-resident memory CD8 T cells correlated with a distinct risk of relapse following surgery by Syrine Abdeljaoued, Alexandre Doussot, Marie Kroemer, Emilien Laloy, Jean René Pallandre, Antoine El Kaddissi, Laurie Spehner, Myriam Ben Khelil, Adeline Bouard, Virginie Mougey, Ugo Chartral, Angélique Vienot, Julien Viot, Zaher Lakkis, Franck Monnien, Romain Loyon, Christophe Borg

“…The prognostic value of TRM cells was also assessed in a well-defined cohort of CRC liver metastases. Here we identified two subsets of TRM cells expressing CD103 and/or CD69 showing significantly higher expression of tissue residency and activation biomarkers. …”
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Repetitive antigen stimulation in the periphery dictates the composition and recall responses of brain-resident memory CD8+ T cells by Madison R. Mix, Stephanie van de Wall, Mohammad Heidarian, Elizabeth A. Escue, Cori E. Fain, Lecia L. Pewe, Lisa S. Hancox, Sahaana A. Arumugam, Cassie M. Sievers, Vladimir P. Badovinac, John T. Harty

“…However, the impact of repeated peripheral viral infection on the generation, phenotype, localization, and recall responses of brain TRM remains elusive. Here, utilizing two murine models of peripheral viral infection, we demonstrate that circulating memory CD8+ T cells with previous antigen exposure exhibit a markedly reduced capacity to form brain TRM compared to naive CD8+ T cells. …”
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An Overview of the Environmental Applicability of Vermicompost: From Wastewater Treatment to the Development of Sensitive Analytical Methods by Madson de Godoi Pereira, Lourdes Cardoso de Souza Neta, Maurício Paulo Ferreira Fontes, Adriana Nascimento Souza, Thaionara Carvalho Matos, Raquel de Lima Sachdev, Arnaud Victor dos Santos, Marluce Oliveira da Guarda Souza, Marta Valéria Almeida Santana de Andrade, Gabriela Marinho Maciel Paulo, Joselito Nardy Ribeiro, Araceli Verónica Flores Nardy Ribeiro

“…The use of vermicompost (humified material) for treating wastewaters, remediating polluted soils, improving agricultural productivity, protecting crop production, and developing sensitive analytical methods is reviewed here, covering the past 17 years. The main advantages of vermicompost, considering all applications covered in this paper, comprise (i) easy acquisition, (ii) low costs, (iii) structural, chemical, and biological characteristics responsible for exceptional adsorptive capacities as well as pollutant degradation, and (iv) the promotion of biocontrol. …”
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Preclinical model for evaluating human TCRs against chimeric syngeneic tumors by Olivier Michielin, Philippe Guillaume, Melita Irving, Patrick Reichenbach, Vincent Zoete, Elise Gray-Gaillard, Evangelos Stefanidis, Aikaterini Semilietof, Julien Pujol, Alessia D'Esposito

“…A variety of humanized mouse models have been described but they have limitations in immune reconstitution and are technically challenging to implement. Here, we have developed a chimeric syngeneic tumor model in which A2Kb transgenic C57BL/6 mice are engrafted with B16 expressing A2Kb:NY as a single chain trimer (SCT) and treated by ACT with murine T cells expressing A2/NY TCRs comprising human variable fused to mouse constant regions.Methods We compared the function of a supraphysiological affinity A2/NY TCR (wtc51m), a computationally designed TCR in an optimal affinity range (DMβ), and a near non-binding TCR (V49I), engineered in both primary human and murine T cells by lentiviral and retroviral transduction, respectively. …”
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Strontium–Alix interaction enhances exosomal miRNA selectively loading in synovial MSCs for temporomandibular joint osteoarthritis treatment by Wenxiu Yuan, Jiaqi Liu, Zhenzhen Zhang, Chengxinyue Ye, Xueman Zhou, Yating Yi, Yange Wu, Yijun Li, Qinlanhui Zhang, Xin Xiong, Hengyi Xiao, Jin Liu, Jun Wang

“…Addressing lower strontium (Sr) levels in arthritic synovial microenvironment, we studied the effect of Sr element on exosomes and miRNA selectively loading in synovial mesenchymal stem cells (SMSCs). Here, we developed an optimized system that boosts the yield of SMSC-derived exosomes (SMSC-EXOs) and improves their miRNA profiles with an elevated proportion of beneficial miRNAs, while reducing harmful ones by pretreating SMSCs with Sr. …”
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Efficacy and safety of surufatinib plus toripalimab in treatment-naive, PD-L1-positive, advanced or metastatic non-small-cell lung cancer and previously treated small-cell lung can... by Ying Cheng, Panpan Zhang, Ming Lu, Zhendong Chen, Lijie Song, Si Shi, Feng Ye, Xing Zhang, Baorui Liu, Dongmei Ji, Yanqiao Zhang, Weiguo Su, Michael Shi, Songhua Fan, Panfeng Tan, Chen Zhong

“…Abstract Background Combining the programmed death-1 inhibitor toripalimab and the angio-immuno kinase inhibitor surufatinib showed preliminary antitumor activity in patients with advanced solid tumors in a phase I study. Here, we report the efficacy and safety of this combination regimen in treatment-naive advanced or metastatic non-small-cell lung cancer (NSCLC) patients with a programmed death-ligand 1 (PD-L1) tumor proportion score (TPS) of 1% or greater (PD-L1-positive) and patients with previously treated small-cell lung cancer (SCLC). …”
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Emotional words evoke region- and valence-specific patterns of concurrent neuromodulator release in human thalamus and cortex by Seth R. Batten, Alec E. Hartle, Leonardo S. Barbosa, Beniamino Hadj-Amar, Dan Bang, Natalie Melville, Tom Twomey, Jason P. White, Alexis Torres, Xavier Celaya, Samuel M. McClure, Gene A. Brewer, Terry Lohrenz, Kenneth T. Kishida, Robert W. Bina, Mark R. Witcher, Marina Vannucci, Brooks Casas, Pearl Chiu, Pendleton R. Montague, William M. Howe

“…Work from model organisms suggests that valence assignments are carried out in part by the neuromodulators dopamine, serotonin, and norepinephrine. Here, we ask whether valence signaling by these neuromodulators extends to word semantics in humans by measuring sub-second neuromodulator dynamics in the thalamus (N = 13) and anterior cingulate cortex (N = 6) of individuals evaluating positive, negative, and neutrally valenced words. …”
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Distinguishable Immunologic Characteristics of COVID-19 Patients with Comorbid Type 2 Diabetes Compared with Nondiabetic Individuals by Ruxing Zhao, Yujing Sun, Yongyuan Zhang, Weili Wang, Shouyu Wang, Chuang Wang, Jinbo Liu, Ling Gao, Zhao Hu, Jianchun Fei, Xinguo Hou, Huizhen Zheng, Li Chen

“…The immune system poses the critical interactive chain between the human body and the virus. Here, we make efforts to examine whether comorbidity with type 2 diabetes (T2D) affects the immunological response in COVID-19 patients. …”
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