Robust collection and processing for label-free single voxel proteomics by Reta Birhanu Kitata, Marija Velickovic, Zhangyang Xu, Rui Zhao, David Scholten, Rosalie K. Chu, Daniel J. Orton, William B. Chrisler, Tong Zhang, Jeremy V. Mathews, Benjamin M. Bumgarner, Demirkan B. Gursel, Ronald J. Moore, Paul D. Piehowski, Tao Liu, Richard D. Smith, Huiping Liu, Clive H. Wasserfall, Chia-Feng Tsai, Tujin Shi

“…However, such bulk measurement lacks spatial resolution and obscures tissue heterogeneity, precluding proteome mapping of tissue microenvironment. Here we report an integrated wet collection of single microscale tissue voxels and Surfactant-assisted One-Pot voxel processing method termed wcSOP for robust label-free single voxel proteomics. wcSOP capitalizes on buffer droplet-assisted wet collection of single voxels dissected by LCM to the tube cap and SOP voxel processing in the same collection cap. …”
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NKp46 enhances type 1 innate lymphoid cell proliferation and function and anti-acute myeloid leukemia activity by Rui Ma, Zhenlong Li, Hejun Tang, Xiaojin Wu, Lei Tian, Zahir Shah, Ningyuan Liu, Tasha Barr, Jianying Zhang, Sean Wang, Srividya Swaminathan, Guido Marcucci, Yong Peng, Michael A. Caligiuri, Jianhua Yu

“…Abstract NKp46 is a critical regulator of natural killer (NK) cell immunity, but its function in non-NK innate immune cells remains unclear. Here, we show that NKp46 is indispensable for expressing IL-2 receptor-α (IL-2Rα) by non-NK liver-resident type-1 innate lymphoid cells (ILC1s). …”
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Interstellar Pickup Ion Observations to 60 au by D. J. McComas, B. L. Shrestha, G. Livadiotis, J. R. Szalay, E. J. Zirnstein, H. A. Elliott, P. C. Brandt, A. R. Poppe, K. N. Singer, A. Stern, A. Verbiscer, the New Horizons Heliophysics Team

“…Prior to the Solar Wind Around Pluto (SWAP) observations from New Horizons, the only significant interstellar pickup ion (PUI) observations were taken inside 5.4 au from the Ulysses and 1 au missions. Here, we analyze SWAP data to provide the first comprehensive radial profile of interstellar PUIs in the outer heliosphere from ∼22 to 60 au. …”
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Case of Superficial Cancer Located at the Pharyngoesophageal Junction Which Was Dissected by Endoscopic Laryngopharyngeal Surgery Combined with Endoscopic Submucosal Dissection by Kenro Kawada, Tatsuyuki Kawano, Taro Sugimoto, Kazuya Yamaguchi, Yuudai Kawamura, Toshihiro Matsui, Masafumi Okuda, Taichi Ogo, Yuuichiro Kume, Yutaka Nakajima, Andres Mora, Takuya Okada, Akihiro Hoshino, Yutaka Tokairin, Yasuaki Nakajima, Ryuhei Okada, Yusuke Kiyokawa, Fuminori Nomura, Takahiro Asakage, Ryo Shimoda, Takashi Ito

“…Its efficacy is reported here. Material and Methods. An 88-year-old woman complaining of dysphagia, diagnosed with cervical esophageal cancer, and hoping to preserve her voice and swallowing function was admitted to our hospital. …”
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Data-driven Dynamics with Orbital Torus Imaging: A Flexible Model of the Vertical Phase Space of the Galaxy by Adrian M. Price-Whelan, Jason A. S. Hunt, Danny Horta, Micah Oeur, David W. Hogg, Kathryn Johnston, Lawrence Widrow

“…These orbits can be used to infer a mass model for the Milky Way, enabling constraints on the dark matter distribution in the inner galaxy. Here, we present a flexible model for foliating the vertical position–velocity phase space with orbits for use in data-driven studies of dynamics. …”
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JAUNOSIOS KARTOS KATALIKŲ INTELEKTUALŲ POŽIŪRIS Į FAŠIZMĄ IR NACIONALSOCIALIZMĄ TARPUKARIO LIETUVOJE by Valdas Pruskus

“…The consequences of this crisis were mostly suffered by Italy and Germany. Exactly here the new standpoint on democracy, a state and its functioning was being formed. …”
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Tuning the selectivity of NH3 oxidation via cooperative electronic interactions between platinum and copper sites by Lu Chen, Xuze Guan, Zhaofu Fei, Hiroyuki Asakura, Lun Zhang, Zhipeng Wang, Xinlian Su, Zhangyi Yao, Luke L. Keenan, Shusaku Hayama, Matthijs A. van Spronsen, Burcu Karagoz, Georg Held, Christopher S. Allen, David G. Hopkinson, Donato Decarolis, June Callison, Paul J. Dyson, Feng Ryan Wang

“…However, achieving high conversion over a wide operating temperature range while avoiding over-oxidation to NOx remains a significant challenge. Here, we report a bi-metallic surficial catalyst (PtSCuO/Al2O3) with improved Pt atom efficiency that overcomes the limitations of current catalysts. …”
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Tannic acid-loaded inorganic-organic coating on magnesium bone nails for enhanced antibacterial, anti-inflammatory and corrosion resistance by Sirui Xu, Chuanyao Dong, Xiaolin Yu, Tianshu Lv, Dapeng Zeng, Ruiyan Li, Xingfu Bao, Kan Zhang

“…However, its excessive corrosion rate and susceptibility to bacterial infection have significantly limited its use as an intramedullary nailing material. Here, we develop a dual-layer structural coating with the micro-arc oxidation layer and tannic acid-doped chitosan layer (TA-CS/MAO) on the magnesium alloy from bottom to up. …”
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A new approach to continuous monitoring of carbon use efficiency and biosynthesis in soil microbes from measurement of CO₂ and O₂ by K. E. Smart, D. O. Breecker, C. B. Blackwood, C. B. Blackwood, C. B. Blackwood, T. M. Gallagher

“…The fraction of carbon consumed by microbes that is used for biomass growth (the carbon use efficiency or CUE) is an important variable controlling soil carbon stocks but is difficult to measure. Here we show that CUE can be continuously monitored in laboratory glucose-amended soil incubations by measuring CO<span class="inline-formula">₂</span> and O<span class="inline-formula">₂</span> gas concentrations, allowing instantaneous estimates of microbial biomass growth. …”
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Systematic underestimation of type-specific ecosystem process variability in the Community Land Model v5 over Europe by C. Poppe Terán, C. Poppe Terán, C. Poppe Terán, B. S. Naz, B. S. Naz, H. Vereecken, H. Vereecken, R. Baatz, R. A. Fisher, H.-J. Hendricks Franssen, H.-J. Hendricks Franssen

“…Past studies have shown the ability of CLM5 to model ET and GPP magnitudes well but emphasized systematic underestimations and lower variability than in the observations.</p> <p>Here, we evaluated CLM5's predictions of water and energy fluxes using observations from eddy covariance stations from the Integrated Carbon Observation System (ICOS), remote sensing, and reanalysis data sets. …”
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Glucose‐induced LINC01419 reprograms the glycolytic pathway by recruiting YBX1 to enhance PDK1 mRNA stability in hepatocellular carcinoma by Yanfang Liu, Junjiao Song, Qili Shi, Bing Chen, Wenying Qiu, Yizhe Liu, Shenglin Huang, Xianghuo He

“…The mechanism and therapeutic potential of cancer‐specific lncRNAs in modulating tumour glucose metabolism warrant in‐depth exploration. Here we revealed that glucose‐induced LINC01419 promoted the growth and metastasis of HCC cells by driving metabolic reprogramming. …”
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Derivation of atmospheric reaction mechanisms for volatile organic compounds by the SAPRC mechanism generation system (MechGen) by W. P. L. Carter, J. Jiang, J. J. Orlando, K. C. Barsanti, K. C. Barsanti, K. C. Barsanti

“…Many of the estimation methods discussed here have not been published previously, and others have not been used previously in developing comprehensive mechanisms. …”
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Spatial and Temporal Variations in 94-GHz Radar Backscatter From a Springtime Snowpack by William D. Harcourt, Duncan A. Robertson, David G. Macfarlane, Brice R. Rea, Mike R. James, Mark Diggins, Blair Fyffe

“…Measuring the interaction between atmospheric conditions and a snowpack at high spatial and temporal resolution requires the use of close-range sensors. Here, we measured the variability of a spring snowpack across two corries in Scotland using ground-based 94 GHz radar in order to assess its ability to monitor snowpack changes. …”
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Frequency multiplexed photothermal correlation tomography for non-destructive evaluation of manufactured materials by Pengfei Zhu, Rongbang Wang, Koneswaran Sivagurunathan, Stefano Sfarra, Fabrizio Sarasini, Clemente Ibarra-Castanedo, Xavier Maldague, Hai Zhang, Andreas Mandelis

“…However, two-dimensional imaging modality limits its development. Here, a technique named frequency multiplexed photothermal correlation tomography (FM-PCT) was developed to enable non-destructive and contactless cross-sectional imaging for manufactured material evaluation and characterization. …”
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A bioinformatic search for correspondence between differentially expressed genes of domestic versus wild animals and orthologous human genes altering reproductive potential by M. P. Ponomarenko, I. V. Chadaeva, P. M. Ponomarenko, A. G. Bogomolov, D. Yu. Oshchepkov, E. B. Sharypova, V. V. Suslov, A. V. Osadchuk, L. V. Osadchuk, Yu. G. Matushkin

“…., growth of the number of humans in the absence of restrictions caused by limiting factors. Here, we applied this model to 68 human genes whose changes in expression alter the reproductive health of women and men and to 3080 DEGs of domestic versus wild animals. …”
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How does gut microbiota affect the vaginitis axis? The mediating role of plasma metabolites by Mo Li, Qianyu Zhang, Tong Wu, Lanfang Ma, Dianxing Hu, Zixuan Yuan, Shixuan Wang, Aiyue Luo, Jinjin Zhang

“…However, the underlying etiological factors remain poorly understood, leading to recurrent vaginitis and constraining clinical management. Here, we explored whether the gut microbiota influences the risk of vaginitis by performing a two-sample Mendelian randomization analysis using the largest genome-wide association studies to date. …”
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An electrolyte-gated transistor for the monitoring of a CRISPR/Cas activity by Pierre Guermonprez, Le Tu Anh, Louis Renaud, Pierre Nioche, Eric Krejci, Aurélie Alleaume-Butaux, Nicolas Battaglini, Vu Thi Thu, Sébastien Sanaur, Benoît Piro

“…Reverse transcriptase-polymerase chain reaction (RT-PCR) remains the gold standard, but clustered regularly interspaced short palindromic repeats linked to a Cas endoribonuclease particle (CRISPR/Cas) have recently revolutionized the recognition step of two RNAs, i.e. the CRISPR-RNA (crRNA) and the target, providing a much better selectivity compared to the naked hybridization on which RT-PCR is based. Here, we combine the high efficiency of the CRISPR/Cas13a system with the transduction and amplification capabilities of an electrolyte-gated graphene field-effect transistor (EGGFET) for the detection of specific RNA sequences. …”
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Two-dimensional anion-rich NaCl2 crystal under ambient conditions by Ruobing Yi, Jie Jiang, Yizhou Yang, Yueyu Zhang, Siyan Gao, Yimin Zhao, Jiahao Hu, Xuchang Su, Xinming Xia, Bingquan Peng, Fangfang Dai, Pei Li, Zhao Guan, Haijun Yang, Fangyuan Zhu, Jiefeng Cao, Zhe Wang, Haiping Fang, Lei Zhang, Liang Chen

“…However, only a few 2D anion-rich “sandwich” materials have been experimentally discovered and fabricated, as they are vastly limited by their conventional stoichiometric ratios and structural stability under ambient conditions. Here, we report a 2D anion-rich NaCl2 crystal with sandwiched structure confined within graphene oxide membranes with positive surface potential. …”
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Assessing the suitability of sites near Pine Island Glacier for subglacial bedrock drilling aimed at detecting Holocene retreat–readvance by J. S. Johnson, J. Woodward, I. Nesbitt, K. Winter, S. Campbell, K. A. Nichols, R. A. Venturelli, S. Braddock, B. M. Goehring, B. Hall, D. H. Rood, G. Balco, G. Balco

“…Such efforts are needed to understand the significance and potential future reversibility of ongoing and projected change in Antarctica. Here we evaluate the suitability for subglacial bedrock drilling of sites in the Hudson Mountains, which are located in the Amundsen Sea sector of West Antarctica. …”
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Anti-proteolytic regulation of KRAS by USP9X/NDRG3 in KRAS-driven cancer development by Han Koo, Kyung Chan Park, Hyun Ahm Sohn, Minho Kang, Dong Joon Kim, Zee-Yong Park, Sehoon Park, Sang Hyun Min, Seong-Hwan Park, Yeon-Mi You, Yohan Han, Bo-Kyung Kim, Chul-Ho Lee, Yeon-Soo Kim, Sang J. Chung, Young Il Yeom, Dong Chul Lee

“…Abstract Cancers with activating mutations of KRAS show a high prevalence but remain intractable, requiring innovative strategies to overcome the poor targetability of KRAS. Here, we report that KRAS expression is post-translationally up-regulated through deubiquitination when the scaffolding function of NDRG3 (N-Myc downstream-regulated gene 3) promotes specific interaction between KRAS and a deubiquitinating enzyme, USP9X. …”
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