Mucosal Immunization with an Influenza Vector Carrying SARS-CoV-2 N Protein Protects Naïve Mice and Prevents Disease Enhancement in Seropositive Th2-Prone Mice by Mariia V. Sergeeva, Kirill Vasilev, Ekaterina Romanovskaya-Romanko, Nikita Yolshin, Anastasia Pulkina, Daria Shamakova, Anna-Polina Shurygina, Arman Muzhikyan, Dmitry Lioznov, Marina Stukova

“…Influenza vectors with truncated NS1 are a promising vaccine approach that ensures a pronounced local CD8+ T-cellular immune response. Here, we describe the protective and immunomodulating properties of an influenza vector FluVec-N carrying the C-terminal fragment of the SARS-CoV-2 nucleoprotein within a truncated NS1 open reading frame. …”
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Insights into a Renowned Fishery: Distinct Spatial Patterns, Environmental Predictors and Species Associations of Trophy-Class Spotted Seatrout (<i>Cynoscion nebulosus</i> [Cuvier... by Catherine Eckert, Elliot Briell, Ethan Getz

“…With recent regulation changes intended to reduce the harvest mortality of trout and promote a healthy trophy-class trout fishery in Texas, understanding differences between trophy-class (>28 in, 712 mm) and adult-class (12–28 in, 305–710) trout has become especially relevant for fisheries managers. Here, we leveraged a long-term (1983–2023) gill net dataset collected by the Texas Parks and Wildlife Department to (1) compare the distribution of size classes both spatially and temporally, (2) determine environmental drivers of the abundance of each size class, and (3) compare the species associated with each size class in the Upper Laguna Madre. …”
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CDC20‐Mediated Selective Autophagy Degradation of PBRM1 Affects Immunotherapy for Renal Cell Carcinoma by Yizeng Fan, Weichao Dan, Taotao Que, Yi Wei, Bo Liu, Zixi Wang, Yulin Zhang, Yuzhao Wang, Tianjie Liu, Yanxin Zhuang, Mengxing Li, Chendong Guo, Jin Zeng, Bohan Ma, Lei Li

“…However, whether targeting PBRM1 has the potential to enhance immunotherapy efficacy in patients with wild‐type PBRM1 and the upstream pathways that regulate PBRM1 protein stability remain unclear. Here, it is demonstrated that PBRM1 knockdown induced M1 macrophage polarization and infiltration, which enhanced the efficacy of anti‐PD‐1 immunotherapy in RCC. …”
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Novel Inhibitors for MDM2-MDM4 E3 Ligase Potently Induce p53-Indepedent Apoptosis in Drug-Resistant Leukemic Cells by Rati Lama, Joseph M. Fose, Diana Martín, Inés G. Muñoz, Eunice S. Wang, Pamela J. Sung, Sherry R. Chemler, Xinjiang Wang

“…Owing to the importance of the E3 ligase of MDM2 in its p53-independent oncogenic activity, inhibitors targeting the E3 ligase activity of MDM2-MDM4 are desirable for p53-mutant cancer cells. Here, we report the development of such inhibitors with pro-apoptotic activity in p53-null leukemic cells. …”
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Disentangling the effects of eutrophication and natural variability on macrobenthic communities across French coastal lagoons by Jones, Auriane G., Schaal, Gauthier, Boyé, Aurélien, Creemers, Marie, Derolez, Valérie, Desroy, Nicolas, Fiandrino, Annie, Mouton, Théophile L., Simier, Monique, Smith, Niamh, Ouisse, Vincent

“…Because coastal lagoons are naturally highly variable in their environmental conditions, disentangling the effects of anthropogenic disturbances like eutrophication from those of natural variability is a challenging, yet necessary issue to address. Here, we analyze a dataset composed of macrobenthic invertebrate abundances and environmental variables (hydro-morphology, water, sediment and macrophytes) gathered across 29 Mediterranean coastal lagoons located in France, to characterize the main drivers of community composition and structure. …”
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Exogenous dihomo-γ-linolenic acid triggers ferroptosis via ACSL4-mediated lipid metabolic reprogramming in acute myeloid leukemia cells by Xiandong Jiang, Yingying Huang, Xiaoying Hong, Wei Wu, Yanfeng Lin, Liping Lin, Yan Xue, Donghong Lin

“…However, few studies have been reported on the lipid metabolism of ferroptosis in acute myeloid leukemia (AML). Here, we analyzed the relationship between lipid metabolism and ferroptosis in AML cells to explore new clinical treatment strategies. …”
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Divergent roles of m6A in orchestrating brown and white adipocyte transcriptomes and systemic metabolism by Ling Xiao, Dario F. De Jesus, Cheng-Wei Ju, Jiang-Bo Wei, Jiang Hu, Ava DiStefano-Forti, Valeria Salerno Gonzales, Tadataka Tsuji, Siying Wei, Matthias Blüher, Yu-Hua Tseng, Chuan He, Rohit N. Kulkarni

“…Abstract N6-methyladenosine (m6A) is among the most abundant mRNA modifications, yet its cell-type-specific regulatory roles remain unclear. Here we show that m6A methyltransferase-like 14 (METTL14) differentially regulates transcriptome in brown versus white adipose tissue (BAT and WAT), leading to divergent metabolic outcomes. …”
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Advanced tissue technologies of blood-brain barrier organoids as high throughput toxicity readouts in drug development by Luisa Bell, Claire Simonneau, Chiara Zanini, Elena Kassianidou, Christelle Zundel, Rachel Neff, Bernd Steinhuber, Marco Tecilla, Alex Odermatt, Roberto Villaseñor, Nadine Stokar-Regenscheit

“…However, their application in drug development, and especially for the regulatory purposes of toxicity assessment, requires robust and reproducible techniques. Here, we developed an adapted set of orthogonal image-based tissue methods including hematoxylin and eosin staining (HE), immunohistochemistry (IHC), multiplex immunofluorescence (mIF), and Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) to validate CIVMs for drug toxicity assessments. …”
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Annual cycle variations in the gut microbiota of migratory black-necked cranes by Yujia Zhang, Ruifeng Ma, Suolangduoerji, Shujuan Ma, Shujuan Ma, Akebota Nuertai, Ke He, Hongyi Liu, Ying Zhu

“…However, the annual dynamics of gut microbiota in migratory birds remain unclear, hindering our understanding of their environmental adaptation.MethodsHere, we collected fecal samples from black-necked cranes (Grus nigricollis) across four seasons at their breeding grounds and used wintering ground data from databases to characterize their gut microbial compositions throughout the year.Results and discussionThe results showed that the gut microbiota was clustered by season (Bray-Curtis: R2 = 0.348, p &lt; 0.001; UniFrac: R2 = 0.352, p &lt; 0.001). …”
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Effect of Lanthanum‐Aluminum Co‐Doping on Structure of Hafnium Oxide Ferroelectric Crystals by Zhenhai Li, Shuqi Tang, Tianyu Wang, Yongkai Liu, Jialin Meng, Jiajie Yu, Kangli Xu, Ruihong Yuan, Hao Zhu, Qingqing Sun, Shiyou Chen, David Wei Zhang, Lin Chen

“…Abstract Hafnium oxide (HfO2)‐based devices have been extensively evaluated for high‐speed and low‐power memory applications. Here, the influence of aluminum (Al) and lanthanum (La) co‐doping HfO2 thin films on the ferroelectric characteristics of hafnium‐based devices is investigated. …”
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Genomic 8-oxoguanine modulates gene transcription independent of its repair by DNA glycosylases OGG1 and MUTYH by Tobias Obermann, Teri Sakshaug, Vishnu Vignesh Kanagaraj, Andreas Abentung, Mirta Mittelstedt Leal de Sousa, Lars Hagen, Antonio Sarno, Magnar Bjørås, Katja Scheffler

“…It has been suggested that OG and its repair has epigenetic-like properties and mediates transcription, but genome-wide evidence of this interdependence is lacking. Here, we applied an improved OG-sequencing approach reducing artificial background oxidation and RNA-sequencing to correlate genome-wide distribution of OG with gene transcription in OGG1 and/or MUTYH-deficient cells. …”
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Psychometric characteristics and feasibility of microinteraction-based Ecological Momentary Assessment in audiology research by Yu-Hsiang Wu, Elizabeth Stangl, Samuel Smith, Jacob Oleson, Christi Miller, Octav Chipara

“…The objectives of the two studies presented here were to evaluate micro-EMA's psychometric characteristics and feasibility across three response formats (2-point, 5-point, and 10-point scales) for adults with hearing loss.DesignIn the first study, thirty-two participants completed a dual-task experiment aimed at assessing the construct validity, responsiveness, intrusiveness, and test-retest reliability of micro-EMA across the three response formats. …”
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Focal therapy of prostate cancer: Use of artificial intelligence to define tumour volume and predict treatment outcomes by Wayne G. Brisbane, Alan M. Priester, Anissa V. Nguyen, Mark Topoozian, Sakina Mota, Merdie K. Delfin, Samantha Gonzalez, Kyla P. Grunden, Shannon Richardson, Shyam Natarajan, Leonard S. Marks

“…TV was compared against conventional baseline variables as a correlate of a successful primary outcome—defined here as the absence of GG ≥ 2 on follow‐up MRGB at 6 months. …”
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Modelling GNSS-observed seasonal velocity changes of the Ross Ice Shelf, Antarctica, using the Ice-sheet and Sea-level System Model (ISSM) by F. Baldacchino, F. Baldacchino, N. R. Golledge, M. Morlighem, H. Horgan, H. Horgan, H. Horgan, A. V. Alevropoulos-Borrill, A. Malyarenko, A. Malyarenko, A. Gossart, D. P. Lowry, L. van Haastrecht

“…However, the drivers of this variability remain poorly understood. Here, we present three new velocity datasets from Global Navigation Satellite System (GNSS) stations on the Ross Ice Shelf collected between early 2020 and late 2021 and show that they have two distinct peaks observed in austral summer and austral winter. …”
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Abundance, occurrence, and degradation of airborne antibiotic resistance genes in coastal and marine atmospheres by Shijie Jia, Shijie Jia, Xiaohong Yao, Jianhua Qi, Jianhua Qi, Xiaohuan Liu, Xiaohuan Liu, Huiwang Gao, Huiwang Gao

“…Airborne ARGs are reportedly widely distributed across inland cities, but little is known about their abundance in marine atmospheres. Here, we report observations of ambient ARGs during a cruise over the marginal seas of the Bohai Sea and Yellow Sea and compare them with ARGs in the coastal atmosphere. …”
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Comparative assessment of Mini-FLOTAC, McMaster and semi-quantitative flotation for helminth egg examination in camel faeces by Khalid M. Mohammedsalih, Salma A. Hassan, Fathel-Rahman Juma, Shamsaldeen I. Saeed, Ahmed Bashar, Georg von Samson-Himmelstjerna, Jürgen Krücken

“…For camels, no evaluations of coproscopic methods regarding precision, sensitivity and correlation between individual and pooled faecal samples are currently available. Methods Here, 410 camel faecal samples were collected in 2022 from South Darfur State, Sudan, and analysed to compare the semi-quantitative flotation, McMaster and Mini-FLOTAC methods in terms of precision, sensitivity, inter-rater reliability and helminth egg count correlations, as well as the effects of pooling samples. …”
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Towards equitable carbon responsibility: Integrating trade-related emissions and carbon sinks in urban decarbonization by Junliang Wu, Yafei Wang, Shuya Zhang, Yu Zhu, Bingyue Fu, Zhihui Zhang, Hanxi Chen, Shaoqing Chen

“…However, the combined impact of trade-related carbon leakage and forest carbon sinks on CMR allocation remains poorly understood. Here, we develop an integrated CMR allocation framework that accounts for both carbon leakage and variation of forest carbon offsets. …”
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Non‐Invasive Photoacoustic Cerebrovascular Monitoring of Early‐Stage Ischemic Strokes In Vivo by Jiwoong Kim, Joo Young Kweon, Seongwook Choi, Hyunseo Jeon, Minsik Sung, Rongkang Gao, Chengbo Liu, Chulhong Kim, Yong Joo Ahn

“…Photoacoustic computed tomography (PACT), a non‐invasive imaging modality that captures multiparametric high‐resolution images of vessel structures, is well suited for evaluating cerebrovascular structures and their function. Here 3D multiparametric transcranial PACT is implemented to monitor the early stage of a photothrombotic (PT)‐stroke model in living rats. …”
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Modulation of Acid Sphingomyelinase in Melanoma Reprogrammes the Tumour Immune Microenvironment by Emma Assi, Davide Cervia, Laura Bizzozero, Annalisa Capobianco, Sarah Pambianco, Federica Morisi, Clara De Palma, Claudia Moscheni, Paolo Pellegrino, Emilio Clementi, Cristiana Perrotta

“…Since acid sphingomyelinase (A-SMase) downregulation in melanoma was shown to determine a malignant phenotype, we aimed here to elucidate the role of A-SMase in the regulation of tumour immunogenic microenvironment using in vivo melanoma models in which A-SMase was either downregulated or maintained at constitutively high levels. …”
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Impacts of benchmarking choices on inferred model skill of the Arctic–Boreal terrestrial carbon cycle by Jeralyn Poe, Deborah Huntzinger, Nathan Collier, Christopher Schwalm, Jon Wells, Christina Schädel, William J Riley, Stephen Sitch

“…We also evaluate observed and modeled functional relationships between ecosystem respiration and air temperature and between gross primary production and precipitation. Here, we find that the magnitude and shape of the responses are strongly impacted by the choice of observational dataset and the approach used to construct the functional relationship benchmark. …”
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