Molecular and Clinical Characterization of Giardia duodenalis Infection in Preschool Children from Lisbon, Portugal by Filipa Santana Ferreira, Rita Alexandre dos Santos Soares de Bellegarde Machado Sá da Bandeira, Cláudia Alexandra Cecílio de Sampaio Ferreira Constantino, Ana Maria Teixeira Duarte Cancela da Fonseca, Joana da Graça Matias Gomes, Rúben Miguel Lopes Rodrigues, Jorge Luís Marques da Silva Atouguia, Sónia Chavarria Alves Ferreira Centeno-Lima

“…The limited number of cases precluded an association of a determined symptom with an assemblage. The data presented here show the relevance of considering G. duodenalis analysis in children with intestinal complaints even in developed countries.…”
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Identification of novel fructo-oligosaccharide bacterial consumers by pulse metatranscriptomics in a human stool sample by Catherine Prattico, Emmanuel Gonzalez, Lharbi Dridi, Shiva Jazestani, Kristin E. Low, D. Wade Abbott, Corinne F. Maurice, Bastien Castagner

“…Understanding which bacteria consume complex carbohydrates, or glycans, provides a mechanistic link between dietary prebiotics and their beneficial health effects, an essential step for their therapeutic application. Here, we used a pulse metatranscriptomics pipeline to identify bacterial consumers based on glycan metabolism induction in a human stool sample. …”
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Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog by Ting Ye, Jingyuan Li, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Jianyu Wang, H. Rosie Xing

“…However, whether Nr5a2 regulates the self‐renewal of lung CSCs is unknown. Here, we showed the diagnostic and prognostic values of elevated Nr5a2 expression in human lung cancer. …”
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Do serial troponins predict the need for cardiac evaluation in trauma patients after ground-level fall? by Rachael A Callcut, Alyssa R Bellini, James T Ross, Madelyn Larson, Skyler Pearson, Anamaria J Robles

“…However, the high sensitivity of the test may lead to repeat testing that is of little clinical value. Here, we examine the role of serial troponins in predicting the need for further cardiac workup in trauma patients after sustaining a fall.Methods Retrospective review of all adult trauma activations for ground-level fall from January 1, 2021 to December 31, 2021 in patients who were hemodynamically and neurologically normal at presentation. …”
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Automating airborne pollen classification: Identifying and interpreting hard samples for classifiers by Manuel Milling, Simon D.N. Rampp, Andreas Triantafyllopoulos, Maria P. Plaza, Jens O. Brunner, Claudia Traidl-Hoffmann, Björn W. Schuller, Athanasios Damialis

“…To shed some light on this issue, we conducted a sample-level difficulty analysis based on the likelihood for one of the largest automatically-generated datasets of pollen grains on microscopy images and investigated the reason for which certain airborne samples and specific pollen taxa pose particular problems to deep learning algorithms. It is here concluded that the main challenges lie in A) the (partly) co-occurring of multiple pollen grains in a single image, B) the occlusion of specific markers through the 2D capturing of microscopy images, and C) for some taxa, a general lack of salient, unique features. …”
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Reaction kinetics and molecular characterization of the compounds formed by photosensitized degradation of the plastic additive bisphenol A in the atmospheric aqueous phase by Yiting Wang, Qingxin Deng, Yiqun Wang, Pan Li, Biao Jin, Jiangping Liu, Peng Cheng, Marcello Brigante, Dario D’Antuono, Luca Carena, Hui Chen, Davide Vione, Sasho Gligorovski

“…Abstract Bisphenol A (BPA, 4,4'-(propane-2,2-diyl)diphenol) is a common plasticizer that is very widespread in the environment and is also found at significant concentrations in the global oceans, due to contamination by plastics. Here we show that triplet sensitization is an important degradation pathway for BPA in natural surface waters, which could prevail if the water dissolved organic carbon is above 2–3 mgC L−1. …”
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Laser-induced texturing: A sustainable approach to self-cleaning mechanisms in solar panel by Rahool Rai, Mahadzir Ishak, Aiman Bin Mohd Halil, M.M. Quazi, Sudhakar Kumarasamy

“…But coatings consists of hazardous chemicals such as: TiO2, SiO2, ZnO, Si3N4,MgF2 and ZrO2. Therefore, the focus here shifts towards the modern use of laser technology for surface modification. …”
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Effects of Preoperative Sleep Disorders on Anesthesia Recovery and Postoperative Pain in Patients Undergoing Laparoscopic Gynecological Surgery under General Anesthesia by Sicong Yu, Yicheng Xiong, Guangtao Lu, Xiangqing Xiong

“…Sleep disorder dramatically affects people’s physical and mental health. Here, we investigated the effect of preoperative sleep disorders on anesthesia recovery and postoperative pain in patients undergoing laparoscopic gynecological surgery under general anesthesia. 120 patients who underwent elective laparoscopic gynecological surgery under general anesthesia in Taizhou Central Hospital from November 2021 to March 2022 were included. …”
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Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of <i>Haemaphysalis longicornis</i> (Parthenogenesis) by Min-Xuan Liu, Xiao-Pei Xu, Fan-Ming Meng, Bing Zhang, Wei-Gang Li, Yuan-Yuan Zhang, Qiao-Ying Zen, Wen-Ge Liu

“…The life cycle of <i>H. longicornis</i> goes through a tightly regulated life cycle to adapt to the changing host and environment, and these stages of transition are also accompanied by proteome changes in the body. Here, we used the isobaric tags for a relative and absolute quantification (iTRAQ) technique to systematically describe and analyze the dynamic expression of the protein and the molecular basis of the proteome of <i>H. longicornis</i> in seven differential developmental stages (eggs, unfed larvae, engorged larvae, unfed nymphs, engorged nymphs unfed adults, and engorged adults). …”
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Differential use of multiple food sources at a bathyal benthic ecosystem in the central Sagami Bay revealed by amino acid nitrogen isotopic compositions by Hidetaka Nomaki, Yoshito Chikaraishi, Nanako O. Ogawa, Koh Maki, Motohiro Shimanaga, Hisami Suga, Masashi Tsuchiya, Toshi Nagata, Naohiko Ohkouchi

“…Sagami Bay, Japan, is located at the tectonic plate boundary and have complex bottom topography, making multiple sources of organic matter to bathyal deep-sea ecosystems. Here, we applied this method to benthic animals (meiofauna, macrofauna, and megafauna) living at 1430 m deep in the central Sagami Bay, Japan. …”
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Residual ciprofloxacin in chicken manure inhibits methane production in an anaerobic digestion system by Yiting Wang, Qing Liu, Hongli Ran, Pingcai Peng, Yan Wang, Guoliang Peng, Yinbao Wu, Xin Wen

“…However, veterinary antibiotic residues present in chicken manure may affect the AD process. Here, the effects of three types of veterinary antibiotics commonly used in laying hen breeding on AD were explored. …”
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Erythrocyte Shape Abnormalities, Membrane Oxidative Damage, and β-Actin Alterations: An Unrecognized Triad in Classical Autism by Lucia Ciccoli, Claudio De Felice, Eugenio Paccagnini, Silvia Leoncini, Alessandra Pecorelli, Cinzia Signorini, Giuseppe Belmonte, Roberto Guerranti, Alessio Cortelazzo, Mariangela Gentile, Gloria Zollo, Thierry Durand, Giuseppe Valacchi, Marcello Rossi, Joussef Hayek

“…Although red blood cells (RBCs) membrane lipidomics and rheological variables have been reported to be altered, with some suggestions indicating an increased lipid peroxidation in the erythrocyte membrane, to date no information exists on how the oxidative membrane damage may affect cytoskeletal membrane proteins and, ultimately, RBCs shape in autism. Here, we investigated RBC morphology by scanning electron microscopy in patients with classical autism, that is, the predominant ASDs phenotype (age range: 6–26 years), nonautistic neurodevelopmental disorders (i.e., “positive controls”), and healthy controls (i.e., “negative controls”). …”
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Protein identification using Cryo-EM and artificial intelligence guides improved sample purification by Kenneth D. Carr, Dane Evan D. Zambrano, Connor Weidle, Alex Goodson, Helen E. Eisenach, Harley Pyles, Alexis Courbet, Neil P. King, Andrew J. Borst

“…This issue is particularly problematic for self-assembling protein nanomaterials, where unexpected geometries may reflect novel assembly states, cross-contamination, or native proteins originating from the expression host. Here, we used an automated structure-to-sequence pipeline to first identify an unknown co-purifying protein found in several purified designed protein samples. …”
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Southern Ocean control on atmospheric CO₂ changes across late Pliocene Marine Isotope Stage M2 by S. Hou, L. Toebrock, M. van der Linden, F. Rothstegge, M. Ziegler, L. J. Lourens, P. K. Bijl

“…Specifically, questions remain about the nature of a lag of <i>p</i><span class="inline-formula">CO₂</span> relative to benthic foraminiferal <span class="inline-formula"><i>δ</i>¹⁸O</span> in late Pliocene Marine Isotope Stage (MIS) M2 (3300 ka), which was longer than during the Pleistocene. Here, we present a multiproxy paleoceanographic reconstruction of the late Pliocene subtropical–subantarctic zone. …”
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Characterization of subcutaneous and visceral de-differentiated fat cells by Yan Li, Houyu Zhang, Carlos F. Ibáñez, Meng Xie

“…Moreover, whether de-differentiation of mature adipocytes from classical subcutaneous and visceral depots occurs under physiological conditions remains unknown. Methods: Here, we used in vitro ''ceiling culture'', single cell/nucleus RNA sequencing, epigenetic anaysis and genetic lineage tracing to address these unknowns. …”
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Pairs and his role in pattem of trade in Vilnius and Kaunas province in 1861-1914 by Aelita Ambrulevičiūtė

“…Because of the specifics of trade in fairs, wholesale of factory goods didn't spread here even since the middle of the eighties when the number of factories and the amount of industrial goods were increasing. …”
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The ground beneath our feet: A critical reflection on 135 years of landscape evolution models for southern Africa by Rivoningo Khosa, Vela Mbele, Kelly Kirsten, Robyn Pickering

“…This notion of long-term aridity and stability has dominated perspectives on southern African landscape evolution. Here, we present a review of this field, starting with the foundational studies from the late 1890s, which underpin Dart’s hypothesis. …”
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Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue by Sara Nava, Valeria Sordi, Luisa Pascucci, Carlo Tremolada, Emilio Ciusani, Offer Zeira, Moris Cadei, Gianni Soldati, Augusto Pessina, Eugenio Parati, Mark Slevin, Giulio Alessandri

“…Interestingly, in orthopedic diseases, this ready-to-use adipose tissue cell derivative seems to have a prolonged time efficacy even upon a single shot injection into osteoarthritic tissues. Here, we investigated the long-term survival and content of MSCs as well the anti-inflammatory activity of LP and its derived MFAT in vitro, with the aim to better understand a possible in vivo mechanism of action. …”
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Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer by Lukas Klein, Mengyu Tu, Niklas Krebs, Laura Urbach, Daniela Grimm, Muhammad Umair Latif, Frederike Penz, Anna Blandau, Xueyan Wu, Rebecca Diya Samuel, Stefan Küffer, Florian Wegwitz, Nathan Chan, Kazeera Aliar, Foram Vyas, Uday Kishore, Elisabeth Hessmann, Andreas Trumpp, Elisa Espinet, Argyris Papantonis, Rama Khokha, Volker Ellenrieder, Barbara T. Grünwald, Shiv K. Singh

“…However, the underlying mechanisms remain unclear. Here, by combining preclinical models, multi-center clinical, transcriptomic, proteomic, and patient bioimaging data, we identify an interplay between neoplastic intrinsic AP1 transcription factor dichotomy and extrinsic macrophages driving subtype co-existence and an immunosuppressive microenvironment. …”
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Multivariate Statistical Analysis Reveals the Heterogeneity of Lacustrine Tight Oil Accumulation in the Middle Permian Jimusar Sag, Junggar Basin, NW China by Yuce Wang, Jian Cao, Keyu Tao, Xiuwei Gao, Erting Li, Chunhua Shi

“…This heterogeneity, however, is hard to investigate by conventional geological and (organic) geochemical tools and thus is critical and challenging to study. Here, we attempted multivariate statistical analysis to reveal the heterogeneity based on a case study in the lacustrine tight oil accumulation in the middle Permian Lucaogou Formation of the Jimusar sag, Junggar Basin, NW China. …”
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