Changes in African lion demography and population growth with increased protection in a large, prey‐depleted ecosystem by Scott Creel, Matthew S. Becker, Ben Goodheart, Anna Kusler, Kachama Banda, Kambwiri Banda, Milan Vinks, Catherine Sun, Chase Dart, Stephi Matsushima, Ruth Kabwe, Will Donald, Luka Zyambo, Peter Indala, Adrian Kaluka, Clive Chifunte, Craig Reid

“…There is consensus that increased protection from prey depletion will be necessary to reverse the decline of lion populations, but few studies have tested whether increased protection is sufficient to reverse the decline, particularly in the large, open ecosystems where most lions remain. Here, we used an integrated population model to test whether lion demography and population dynamics were measurably improved by increased protection. …”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…However, the function of RA in osteo/odontogenic differentiation of DPSCs remains unclear. Here, we investigated the expression pattern of RA in miniature pig tooth germ and intervened in the RA signal during osteo/odontogenic differentiation of human DPSCs. …”
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Ultrafast laser frequency tuning based on equivalent optical phase sampling and accumulation by Weiqiang Lyu, Huan Tian, Zhenwei Fu, Lingjie Zhang, Zhen Zeng, Yaowen Zhang, Heping Li, Zhiyao Zhang, Yong Liu

“…Nevertheless, the maximum frequency tuning rate and sweep rate are smaller than 107 THz/s and 1 GHz, respectively, due to the inherent speed limitation of the existing tuning mechanisms. Here, we propose and demonstrate a brand-new concept for ultrafast laser frequency tuning, which is realized based on equivalent optical phase sampling and accumulation in the laser cavity. …”
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Good soil management can reduce dietary zinc deficiency in Zimbabwe by Muneta G. Manzeke-Kangara, Edward J. M. Joy, Florence Mtambanengwe, Prosper Chopera, Michael J. Watts, Martin R. Broadley, Paul Mapfumo

“…Agronomic biofortification with Zn fertilizers and improved soil fertility management, using mineral and organic nutrient resources, has previously been shown to increase Zn concentration of staple grain crops, including maize. Here, we show the potential of different soil fertility management options on maize crops to reduce dietary Zn deficiency in Zimbabwe using secondary data from a set of surveys and field experiments. …”
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Secondary motor cortex tracks decision value during the learning of a non-instructed task by Elisabete Augusto, Vladimir Kouskoff, Nicolas Chenouard, Margaux Giraudet, Léa Peltier, Aron de Miranda, Alexy Louis, Lucille Alonso, Frédéric Gambino

“…However, its role in deterministic tasks during initial learning remains uncertain. Here, we describe a self-initiated deterministic task requiring mice to use their forepaws to make choices without guiding cues. …”
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Pan-cancer characterization of m6A-mediated regulation of T cell exhaustion dynamics and clinical relevancies in human cancers by Weiping Ji, Ye Fang, Liwei Chen, Yitong Zheng, Yifei Pei, Changqiu Mei, Meng Zhou

“…The role of N6-methyladenosine (m6A) modification in regulating immune cell function has been recognized, but its impact on TEX dynamics across cancer types and clinical outcomes remains unclear. Here, we conducted a pan-cancer analysis integrating multi-omics data from cell lines, single-cell RNA sequencing, and pan-cancer and immunotherapy datasets to explore the dynamic interplay between m6A modification and TEX. …”
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Enrichment of electrotrophic microorganisms from contrasting shallow-sea hydrothermal environments in bioelectrochemical reactors by Antoine Carissimo, Victoria Comes, Alenica Heussner, Anne-Hélène Prime, Roy E. Price, Gaël Erauso, Pierre-Pol Liebgott, Sven Kerzenmacher, Guillaume Pillot

“…Bioelectrochemical reactors can be used to investigate such electrotrophic diversity by providing an electrode as an electron donor.MethodsHere, a total of 60 different reactors were set up and inoculated with either a microbial community coming from the shallow, acidic (ca. pH 5.5) and hot (ca. 120°C) hydrothermal system of Panarea, Aeolian islands, Italy, or the shallow, alkaline (pH 11) and mild (40°C) hydrothermal system of Prony Bay, New Caledonia.ResultsWith the alkaline sample, no electrical current increase was seen in any of the 15 reactors operated for 6 days under Prony hydrothermal conditions (pH 10, 30–75°C). …”
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Tetrodotoxin, fungal pathogen infection, and bacterial microbiome associations are variable in the skin microecosystems of two Taricha newt species by Talon Jost, Talon Jost, Alysha Henderson, Brandon LaBumbard, Krisztian Magori, Amber Stokes, Danica Bergin, Autumn Holley, Autumn Holley, Molly Bletz, Molly Bletz, Obed Hernández-Gómez, Gary Bucciarelli, Douglas C. Woodhams, Jonah Piovia-Scott, Jenifer B. Walke

“…The interaction of tetrodotoxin with the skin microbiome, including pathogenic fungi, is not well understood, and here we describe these patterns across 12 populations of Taricha granulosa and T. torosa in Washington, Oregon, and California. …”
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Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris by Sibel Kalyoncu, Dogu Sayili, Ayca Zeybek Kuyucu, Hakan Soyturk, Seyda Gullu, Busra Ersayan, Ibrahim Oguzhan Tarman, Mehmet Ender Avci, Olcay Mert, Umut Haskok, Ege Tekin, Huseyin Akinturk, Ridvan Orkut, Aysegul Demirtas, Idil Tilmensagir, Ceren Ulker, Bilgi Gungor, Mehmet Inan

“…Pichia pastoris is an ideal expression system for recombinant protein production due to its cost‐effective and easy‐to‐scale‐up process. Here, we developed a next‐generation SARS‐CoV2 Omicron BA.1‐based recombinant vaccine candidate expressed in P. pastoris. …”
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D-mannose promotes diabetic wound healing through inhibiting advanced glycation end products formation in keratinocytes by Jialiang Luo, Tianxing Wu, Jing Zhang, Zhicheng Liang, Weijie Shao, Di Wang, Lei Li, Daming Zuo, Jia Zhou

“…D-mannose is enriched in cirtus peel and exerts beneficial effects among various diseases, especially against inflammation-related disorders. Methods Here, we examined the potential effect of D-mannose during wound healing process in streptozotocin (STZ)-induced diabetes mice in vivo and by culturing keratinocytes under high glucose condition in vitro. …”
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A bispecific antibody-drug conjugate targeting pCAD and CDH17 has antitumor activity and improved tumor-specificity by Alyssa Synan, Nila C. Wu, Roberto Velazquez, Thomas Gesner, Claude Logel, Kathrin Mueller, Andrew Green, Patrizia Barzaghi-Rinaudo, Quincey Simmons, Samuele Mercan, Xingyi Shi, Xun Li, Vivek Sagar, Joshua Korn, Margaret McLaughlin, William R. Tschantz, Dominik Hainzl, Anthony Malamas, Thomas Huber, Regis Cebe, Kathleen T. Xie, Joseph A. D’Alessio

“…Overall, the preclinical data presented here support the proof-of-concept bispecific antibody discovery approach, demonstrating a rational design for screening antibodies by prioritizing cross-arm avid IgGs to target dual-positive cells.…”
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Efficient Inverted ITO-Free Organic Solar Cells Based on Transparent Silver Electrode with Aqueous Solution-Processed ZnO Interlayer by Zhizhe Wang, Dazheng Chen, Chunfu Zhang, Zhenhua Lin, Yan Liu, Genquan Han, Jingjing Chang, Jincheng Zhang, Lixin Guo, Yue Hao

“…The blend of low bandgap poly[[4,8-bis[(2-ethylhexyl)oxy]benzo[1,2-b:4,5-b′]dithiophene-2,6-diyl][3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4-b]thiophenediyl]] (PTB7) and [6,6]-phenyl-C71-butyric acid methylester (PC71BM) is employed as the photoactive layer here. A power conversion efficiency (PCE) of 5.55% is achieved for such indium tin oxide- (ITO-) free OSCs under AM 1.5G simulated illumination, comparable to that of ITO-based reference OSCs (PCE of 6.11%). …”
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A single-cell and spatial wheat root atlas with cross-species annotations delineates conserved tissue-specific marker genes and regulators by Yuji Ke, Vincent Pujol, Jasper Staut, Lotte Pollaris, Ruth Seurinck, Thomas Eekhout, Carolin Grones, Maite Saura-Sanchez, Michiel Van Bel, Marnik Vuylsteke, Andrea Ariani, Christophe Liseron-Monfils, Klaas Vandepoele, Yvan Saeys, Bert De Rybel

“…Summary: Despite the broad use of single-cell/nucleus RNA sequencing in plant research, accurate cluster annotation in less-studied plant species remains a major challenge due to the lack of validated marker genes. Here, we generated a single-cell RNA sequencing atlas of soil-grown wheat roots and annotated cluster identities by transferring annotations from publicly available datasets in wheat, rice, maize, and Arabidopsis. …”
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Microarray Analysis Reveals a Potential Role of lncRNA Expression in 3,4-Benzopyrene/Angiotensin II-Activated Macrophage in Abdominal Aortic Aneurysm by Yingying Zhou, Jiaoni Wang, Yangjing Xue, Aili Fang, Shaoze Wu, Kaiyu Huang, Luyuan Tao, Jie Wang, Yigen Shen, Jinsheng Wang, Lulu Pan, Lei Li, Kangting Ji

“…We have also demonstrated that macrophage activation plays a key role in Bap-induced AAA, but the mechanism is unknown. Here, we used a mouse lncRNA array to investigate the expression signatures of lncRNAs and mRNAs in Bap-activated macrophage. …”
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Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics by Huifang Li, Ni Deng, Jiayi Yang, Yang Zhao, Xiaoxuan Jin, Ang Cai, Navindra P. Seeram, Hang Ma, Dongli Li, Huilan Yang, Chang Liu, Chang Liu

“…Oleuropein is a phenolic compound commonly found in cosmetic ingredients including olive leaves and jasmine flowers with various skin-beneficial effects. Here, we evaluated oleuropein’s anti-inflammatory and antioxidant activities in human skin cells. …”
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Mechanistic evaluation of enhanced graphene toxicity to Bacillus induced by humic acid adsorption by Xuejiao Zhang, Jin Zeng, Jason C. White, Fangbai Li, Zhiqiang Xiong, Siyu Zhang, Yuze Xu, Jingjing Yang, Weihao Tang, Qing Zhao, Fengchang Wu, Baoshan Xing

“…The humic acid (HA) corona is traditionally considered to reduce GNSs toxicity. Here, we evaluate the effect of sorbed HA (GNSs-HA) on the toxicity of GNSs to Gram positive Bacillus tropicus. …”
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Biglycan-driven risk stratification in ZFTA-RELA fusion supratentorial ependymomas through transcriptome profiling by Konstantin Okonechnikov, David R. Ghasemi, Daniel Schrimpf, Svenja Tonn, Martin Mynarek, Jan Koster, Till Milde, Tuyu Zheng, Philipp Sievers, Felix Sahm, David T.W. Jones, Andreas von Deimling, Stefan M. Pfister, Marcel Kool, Kristian W. Pajtler, Andrey Korshunov

“…However, molecular prognosticators within ST-EPN ZFTA-RELA have not been determined yet. Here, we performed methylation-based DNA profiling and transcriptome RNA sequencing analysis of 80 ST-EPN ZFTA-RELA investigating the clinical significance of various molecular patterns. …”
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Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infect... by Emilio Barba, José Manuel Molina, Francisco Rodríguez, Otilia Ferrer, María Carmen Muñoz, Liliana M. R. Silva, María Cristina Del Río, José Adrián Molina, Anja Taubert, Carlos Hermosilla, Antonio Ruiz

“…The results showed that the Gran Canaria (GC) <i>E. christenseni</i> strain had similar morphological and biological characteristics to those previously described, but no significant clinical signs were observed despite the high oocyst counts here recorded. The novel strain isolated would therefore be of low pathogenicity but still able to develop significant immunoprotective responses upon challenge infections. …”
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Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease by Angel D'Oliviera, Xuhang Dai, Saba Mottaghinia, Sophie Olson, Evan P Geissler, Lucie Etienne, Yingkai Zhang, Jeffrey S Mugridge

“…The SARS-CoV-2 main protease (Mpro or Nsp5) is critical for production of viral proteins during infection and, like many viral proteases, also targets host proteins to subvert their cellular functions. Here, we show that the human tRNA methyltransferase TRMT1 is recognized and cleaved by SARS-CoV-2 Mpro. …”
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Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge by M. Biddle, P. Stylianou, M. Rekas, A. Wright, J. Sousa, D. Ruddy, M. I. Stefana, K. Kmiecik, A. Bandrowski, R.A. Kahn, C. Laflamme, E. M. Krockow, H.S. Virk

“…Researchers frequently use antibodies without confirming that they perform as intended in their application of interest. Here we argue that the determinants of end-user antibody choice and use are critical, and under-addressed, behavioral drivers of this problem. …”
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