Chromosome-level reference genome assembly for the mountain hare (Lepus timidus) by Fekete, Zsófia, Absolon, Dominic E., Michell, Craig, Wood, Jonathan M. D., Goffart, Steffi, Pohjoismäki, Jaakko L. O.

“…We present here a high-quality genome assembly of a male mountain hare (Lepus timidus Linnaeus), from Ilomantsi, Eastern Finland, utilizing an isolated fibroblast cell line as the source for high quality DNA and RNA. …”
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Zinc finger proteins facilitate adaptation of a global insect pest to climate change by Tianpu Li, Jiao Guo, Guilei Hu, Fang Cao, Haiyin Su, Mengdi Shen, Huimin Wang, Minsheng You, Yuanyuan Liu, Geoff M. Gurr, Shijun You

“…As poikilotherms, insect pests such as the globally devastating diamondback moth (DBM), Plutella xylostella, are especially vulnerable to rising temperatures and extreme heat events, necessitating effective adaptive mechanisms. Results Here we demonstrate the roles of zinc finger proteins (ZFPs) in mediating thermal adaptability in DBM. …”
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A mobile-based multidomain lifestyle intervention using Cognitive Evergreenland for older adults with subjective cognitive decline: a feasibility study by Rong Lin, Yuanjiao Yan, Zhili Chen, Chenshan Huang, Junyu Zhao, Mingfeng Chen, Hong Li

“…Subjective cognitive decline (SCD) is present in > 50% of older adults and associated with progression to dementia. Here, we tested the compliance and effectiveness of a Multidomain Lifestyle Intervention Program using the mini-program, Cognitive Evergreenland, (MLIP-CE), based on the Health Action Process Approach model to support home-based intervention in older adults with SCD. …”
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A novel case of glial transdifferentiation in renal medullary carcinoma brain metastasis by Maria A. Gubbiotti, Ian E. McCutcheon, Priya Rao, Giannicola Genovese, Linghua Wang, Artem Tarasov, Vladislav Putintsev, Amber Berlinski, Danil Stupichev, Kirill Kriukov, Suren Davitavyan, Basim Salem, Alexander Sarachakov, Dmitry Lebedev, Michael Hensley, Alexander Bagaev, Francesca Paradiso, Vladimir Kushnarev, Gleb Khegai, Nizar M. Tannir, Pavlos Msaouel

“…Although metastasis to lungs, lymph nodes, and bone is commonly reported, distant spread to the central nervous system almost never occurs. Here we present an unusual case of a patient with renal medullary carcinoma with metastasis to the brain following treatment which included tazemetostat, an EZH2 inhibitor. …”
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Harnessing macrophage-drug conjugates for allogeneic cell-based therapy of solid tumors via the TRAIN mechanism by Bartlomiej Taciak, Maciej Bialasek, Malgorzata Kubiak, Ilona Marszalek, Malgorzata Gorczak, Olha Osadchuk, Daria Kurpiel, Damian Strzemecki, Karolina Barwik, Marcin Skorzynski, Julia Nowakowska, Waldemar Lipiński, Łukasz Kiraga, Jan Brancewicz, Robert Klopfleisch, Łukasz Krzemiński, Emilia Gorka, Anna Smolarska, Irena Padzinska-Pruszynska, Małgorzata Siemińska, Jakub Guzek, Jan Kutner, Marlena Kisiala, Krzysztof Wozniak, Giacomo Parisi, Roberta Piacentini, Luca Cassetta, Lesley M. Forrester, Lubomir Bodnar, Tobias Weiss, Alberto Boffi, Paulina Kucharzewska, Tomasz P. Rygiel, Magdalena Krol

“…Tumor-infiltrating macrophages play a pivotal role in tumor dynamics. Here, we present a Macrophage-Drug Conjugate (MDC) platform technology that enables loading macrophages with ferritin-drug complexes. …”
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Microenvironmental modulation breaks intrinsic pH limitations of nanozymes to boost their activities by Tong Li, Xiaoyu Wang, Yuting Wang, Yihong Zhang, Sirong Li, Wanling Liu, Shujie Liu, Yufeng Liu, Hang Xing, Ken-ichi Otake, Susumu Kitagawa, Jiangjiexing Wu, Hao Dong, Hui Wei

“…However, the deviation between the working and optimal pHs of nanozymes has been limiting their practical applications. Here we develop a strategy to modulate the microenvironmental pHs of metal–organic framework (MOF) nanozymes by confining polyacids or polybases (serving as Brønsted acids or bases). …”
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Methyltransferase-like 7B participates in bladder cancer via ACSL3 m6A modification in a ferroptosis manner by Jiani He, Changming Dong, Xiandong Song, Zhongkai Qiu, Hao Zhang, Yuanjun Jiang, Tao Liu, Xiaojun Man

“…Considering that ferroptosis is emerging as a therapeutic target for cancer, and the possible relationship between METTL7B and antioxidant enzymes. We, here, examined that ectopic METTL7B expression abolished ferroptosis markers in cells raised by Erastin treatment, including the production of lipid ROS, the increased cellular iron and MDA content, the decreased gene expression of ACSL3, FANCD2, and FADS2, as well as the mitochondrial injury observed by electron microscopy. …”
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Dynamic changes in the gastrointestinal microbial communities of Gangba sheep and analysis of their functions in plant biomass degradation at high altitude by Xiaozhen Liu, He Ding, Xiaoxue Zhang, Na Ta, Jinmei Zhao, Qian Zhang, Huiyun Liu, Mengjiao Sun, Xiaoqing Zhang

“…Additionally, the catalogue of microbial genomes and the encoded biomass-degrading enzymes identified here provide insights into the potential microbial functions of the gastrointestinal tract of Gangba sheep at high altitudes. …”
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Spatiotemporal dynamics and influencing factors of native and invasive saltmarshes in a rapidly silting bay during 1985–2023 by Mingshan Xu, Mingshan Xu, Mingshan Xu, Jiali Gu, Peng Yun, Jianjun Gan, Wenzhou Fan, Zhentao Chen, Jie Shao, Guangzhi Zhang, Jian Zeng

“…However, the underlying processes and driving mechanisms behind these trends remain poorly understood, particularly in rapidly silting coastal bays. Here, we investigated the spatiotemporal dynamics and influencing factors of native saltmarshes (Scirpus mariqueter and Phragmites australis) and invasive Spartina alterniflora in Yueqing Bay, eastern China, by interpreting Landsat series images during 1985-2023. …”
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Quantifying Stress and Relaxation: A New Measure of Heart Rate Variability as a Reliable Biomarker by Emese Rudics, András Buzás, Antónia Pálfi, Zoltán Szabó, Ádám Nagy, Emőke Adrienn Hompoth, József Dombi, Vilmos Bilicki, István Szendi, András Dér

“…In order to remedy this problem, here we introduce a novel HRV parameter, the normalized variability derived from an RMSSD “Master Curve”, and we compare it with the conventional metrics. …”
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The role of civil society organisations in initiating an anti-corruption policy in Serbia by Korać Srđan T., Bulatović Aleksandra

“…As the concept of political community is a special construct based on democracy and human rights, non-inclusiveness as previously outlined here destructively affected society by making deeper asymmetries of all kinds due to disintegrating influence that prevents the final community constitution through democratic institutions.…”
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Single-cell transcriptomic analysis reveals characteristic feature of macrophage reprogramming in liver Mallory-Denk bodies pathogenesis by Zixuan Fang, Bei Zhong, Yi Shi, Wanmei Zhou, Maoping Huang, Samuel W. French, Xiaoping Tang, Hui Liu

“…Mallory-Denk bodies (MDBs) are protein aggregates associated with hepatic inflammation, and MDBs pathogenesis could be induced in mice by feeding 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC). Here, we investigate the macrophage heterogeneity and the role of macrophage during MDBs pathogenesis on DDC-induced MDBs mouse model by single-nucleus RNA sequencing (snRNA-seq). …”
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Disposable and flexible smart electronic tapes for long-term biopotential monitoring by Jiuwei Gao, Mingwei Hu, Hao Sun, Yuanyuan Wang, Yuan Wei, Weiwei Li, Lu Zheng, Manzhang Xu, Qianbo Lu, Zhiyuan Liu, Hui Yang, Yongtian Wang, Weitao Song, Xuewen Wang, Wei Huang

“…Repeating the use of electrodes on different individuals can lead to the risk of cross-infection. Here, we propose a smart electronic tape (SET) to acquire biopotentials, in the form of a dry single-sided tape made from a combination of an ultra-stretchable conductive pattern on substrates with different functions. …”
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Targeted detection of sequence variants in cell-free DNA from cerebrospinal fluid in pediatric central nervous system tumors by Katrina O’Halloran, Katrina O’Halloran, Erin E. Crotty, Eirini Christodoulou, Sarah E. Leary, Alexandra Miller, Alexandra Miller, Vera A. Paulson, Christina M. Lockwood, Ashley S. Margol, Ashley S. Margol, Jaclyn A. Biegel, Jaclyn A. Biegel

“…In contrast to broad lower-depth sequencing, commonly referred to as low pass whole genome sequencing (WGS), targeted platforms with a higher depth of coverage have also been established. Here, we review targeted liquid biopsy techniques with applicability to pediatric CNS tumors. …”
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The Holy Grail of Orthopedic Surgery: Mesenchymal Stem Cells—Their Current Uses and Potential Applications by Roberto Berebichez-Fridman, Ricardo Gómez-García, Julio Granados-Montiel, Enrique Berebichez-Fastlicht, Anell Olivos-Meza, Julio Granados, Cristina Velasquillo, Clemente Ibarra

“…MSCs are easily obtained and can be cultivated and expanded in vitro; thus, they represent a promising and encouraging treatment approach in orthopedic surgery. Here, we review the application of MSCs to various orthopedic conditions, namely, orthopedic trauma; muscle injury; articular cartilage defects and osteoarthritis; meniscal injuries; bone disease; nerve, tendon, and ligament injuries; spinal cord injuries; intervertebral disc problems; pediatrics; and rotator cuff repair. …”
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A bivalent COVID-19 mRNA vaccine elicited broad immune responses and protection against Omicron subvariants infection by Jun Liu, Li Wang, Alexandra Kurtesi, Patrick Budylowski, Kyle G. Potts, Haritha Menon, Yilin Tan, Philip Samaan, Xinan Liu, Yisen Wang, Queenie Hu, Reuben Samson, Freda Qi, Danyel Evseev, Cini John, Kristofor K. Ellestad, Yue Fan, Frans Budiman, Ellaine Riczly Tohan, Suji Udayakumar, Jennifer Yang, Eric G. Marcusson, Anne-Claude Gingras, Douglas J. Mahoney, Mario A. Ostrowski, Natalia Martin-Orozco

“…Especially, the protection breadth and degree of these vaccines against antigenically distant Omicron subvariants is unclear. Here, we report the immunogenicity and efficacy of a bivalent mRNA vaccine, PTX-COVID19-M1.2 (M1.2), which encodes native spike proteins from Wuhan-Hu-1 (D614G) and Omicron BA.2.12.1, in mouse and hamster models. …”
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Irregular Pulse and Cardiovascular Disease From NHANES (1999–2018): A Cross‐Sectional Study by Qingping Zeng, Weihong Chang, Rui Zhang, Hongxuan Fan, Aman Liu, Ping Zhang, Fei She, Boda Zhou

“…However, whether irregular pulse was associated with cardiovascular disease (CVD) or mortality has not been reported on a large population scale. Here, we investigated the association between irregular pulse, CVD, and CVD mortality, to explore the potential of irregular pulse as screening indicator for CVD and mortality, thus influencing health policy. …”
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Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro by Klaus Neef, Philipp Treskes, Guoxing Xu, Florian Drey, Sureshkumar Perumal Srinivasan, Tomo Saric, Erastus Nembo, Judith Semmler, Filomain Nguemo, Christof Stamm, Douglas B. Cowan, Antje-Christin Deppe, Maximilian Scherner, Thorsten Wittwer, Jürgen Hescheler, Thorsten Wahlers, Yeong-Hoon Choi

“…Autologous cells with the potential to regenerate damaged heart tissue would be an ideal source for cell therapeutic approaches. Here, we compared different methods of conditional culture for increasing the yield and cardiogenic potential of murine skeletal muscle-derived stem cells. …”
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Association of clinical knowledge with patient care ownership among resident physicians: a nationwide cross-sectional study in Japan by Hirohisa Fujikawa, Hidetaka Tamune, Yuji Nishizaki, Taro Shimizu, Yu Yamamoto, Kiyoshi Shikino, Miwa Sekine, Hiroyuki Kobayashi, Yasuharu Tokuda

“…However, the association between these two concepts is under-examined. Here, we conducted a nationwide cross-sectional study to investigate the association between clinical knowledge and PCO among resident physicians in Japan. …”
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Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi by Mireia Larrosa-Godall, Joshua X. D. Ang, Philip T. Leftwich, Estela Gonzalez, Lewis Shackleford, Katherine Nevard, Rob Noad, Michelle A. E. Anderson, Luke Alphey

“…Methods In this study, a geographically confineable gene drive system targeting dsx was developed (dsx gRNA ). Here, a transgenic line which expresses Cas9 under the control of the endogenous zpg promoter was generated. …”
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