Managing xylazine-involved overdoses in a community harm reduction setting: lessons from Tijuana, Mexico by Lilia Pacheco Bufanda, Alejando González Montoya, Brenda Torres Carrillo, Mariana Alejandra Gonzalez Tejeda, Luis A. Segovia, Alhelí Calderón-Villarreal, Joseph R. Friedman

“…Although it is increasingly found in overdose deaths across North America, the clinical management of xylazine-involved overdoses has not been extensively studied, especially in community-based harm reduction settings. Here we present a clinical series of xylazine-involved overdose and share the clinical approach and lessons learned by a community overdose response team in Tijuana, Mexico amidst the arrival of xylazine. …”
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LIN28A-dependent lncRNA NEAT1 aggravates sepsis-induced acute respiratory distress syndrome through destabilizing ACE2 mRNA by RNA methylation by Jun Liu, Xiang Li, Peng Yang, Yufeng He, Weilong Hong, Yawei Feng, Zhiqiang Ye

“…However, the precise mechanisms underlying sepsis-induced ARDS remain unclear. Methods Here RNA methylation was detected by methylated RNA immunoprecipitation (MeRIP), RNA stability was determined by RNA decay assay while RNA antisense purification (RAP) was used to identify RNA-protein interaction. …”
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Age and moisture affect the relationship between competition and tree growth by Xinyu Han, Lushuang Gao, Mingqian Liu, Yihong Zhu, Keda Cui, Xinyu Zhang, Xiuhai Zhao, Ruibo Zhang, Klaus von Gadow, Yiyu Tian, Kangchen Wang

“…While competition among trees is recognized as a critical factor influencing their growth, the precise mechanism underlying its impact remains unclear. Here, we investigate the role of crown size in the process of competition affecting tree growth across varying gradients of age and moisture conditions. …”
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Interaction Effects of Lipoprotein Lipase Polymorphisms with Lifestyle on Lipid Levels in a Korean Population: A Cross-sectional Study by Jung-A Pyun, Sunshin Kim, KyungChae Park, Inkyung Baik, Nam H Cho, InSong Koh, Jong-Young Lee, Yoon Shin Cho, Young Jin Kim, Min Jin Go, Eugene Shim, KyuBum Kwack, Chol Shin

“…However, studies about the influence of interactions between LPL SNPs and lifestyle factors have not been sufficiently performed. Here, we examine if LPL polymorphisms, as well as their interaction with lifestyle factors, influence lipid concentrations in a Korean population. …”
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Determination of the quantitative content of chlorophylls in leaves by reflection spectra using the random forest algorithm by E. A. Urbanovich, D. A. Afonnikov, S. V. Nikolaev

“…The use of machine learning methods for these purposes is promising, since these methods allow inferring the relationships between input and output variables (prediction model), and in order to improve the quality of the prediction, a researcher may modify predictors and selects a set of method parameters. Here, we present the results of the implementation and evaluation of the random forest algorithm for predicting the total concentration of chlorophylls a and b from the ref lection spectra of plant leaves in the visible and infrared wavelengths. …”
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Multifunctional nanoparticles confers both multiple inflammatory mediators scavenging and macrophage polarization for sepsis therapy by Wenjie Xi, Weijie Wu, Lili Zhou, Qi Zhang, Shushu Yang, Lihong Huang, Yijun Lu, Jing Wang, Xinjin Chi, Yang Kang

“…Thus, cfDNA clearance and down-regulation of inflammatory factors are essential for the effective treatment of sepsis. Here we designed and constructed a polydopamine-based multifunctional nanoparticle for the treatment of sepsis. …”
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A high-valence bismuth(V) nanoplatform triggers cancer cell death and anti-tumor immune responses with exogenous excitation-free endogenous H2O2- and O2-independent ROS generation by Yizhang Tang, Xujiang Yu, Liangrui He, Meng Tang, Wenji Yue, Ruitong Chen, Jie Zhao, Qi Pan, Wanwan Li

“…Abstract Reactive oxygen species with evoked immunotherapy holds tremendous promise for cancer treatment but has limitations due to its dependence on exogenous excitation and/or endogenous H2O2 and O2. Here we report a versatile oxidizing pentavalent bismuth(V) nanoplatform (NaBiVO3-PEG) can generate reactive oxygen species in an excitation-free and H2O2- and O2-independent manner. …”
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Calcitonin Gene-Related Peptide (Cgrp), Adrenomedullin (Am), Amylin, And Calcitonin (Ct) Receptors And Overlapping Biological Actions by J.A. Fischer, W. Born, R. Muff

“…The structure of a CT receptor was elucidated by the group of Goldring in 1991 through molecular cloning, and of a 60% homologous human CT receptor-like receptor (CRLR) shortly thereafter here. The latter was an orphan receptor until the discovery of the receptor-activity-modifying proteins (RAMP) by Foord which upon coexpression yield a CGRP receptor with RAMP1 and an AM receptor with RAMP2. …”
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Mutual control of electric and magnetic orders near room temperature in Al doped Y-type hexaferrite single crystals by Pengzheng Li, Mengfang Yu, Lin Yang, Qiutian Duan, Yinchen Wu, Aihua Zhang, Min Zeng, Meifeng Liu, Xingsen Gao

“…Realizing robust magnetoelectric (ME) coupling effect near room temperature is still a long-standing challenge for the application of multiferroic materials in next-generation low-power spintronic and memory devices. Here we report a systematic study on the magnetic, dielectric, and ME coupling properties of Y-type hexaferrite Ba0.5Sr1.5Co2Fe12–xAlxO22 (x = 0.0, 0.5, 1.0) single crystals. …”
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Linking the life stages of fish into a habitat-ecological flow assessment scheme under climate change and human activities by Yiming Zhang, Wensheng Wang, Siyi Yu

“…Meanwhile, a comprehensive study of ecological flow combining fish habitats with swimming ability and ecological preferences of fish is currently lacking. Here the runoff under different scenarios was firstly acquired, including baseline scenario (BS, with no impact), climate change scenario (CS, impacted by climate change), and variation scenario (VS, impacted by climate change and human activities). …”
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Discovery of robust and highly specific microbiome signatures of non-alcoholic fatty liver disease by Emmanouil Nychas, Andrea Marfil-Sánchez, Xiuqiang Chen, Mohammad Mirhakkak, Huating Li, Weiping Jia, Aimin Xu, Henrik Bjørn Nielsen, Max Nieuwdorp, Rohit Loomba, Yueqiong Ni, Gianni Panagiotou

“…However, finding robust bacterial signatures of NAFLD has been a great challenge, mainly due to its co-occurrence with other metabolic diseases. Results Here, we collected public metagenomic data and integrated the taxonomy profiles with in silico generated community metabolic outputs, and detailed clinical data, of 1206 Chinese subjects w/wo metabolic diseases, including NAFLD (obese and lean), obesity, T2D, hypertension, and atherosclerosis. …”
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Oncolytic alphavirus-induced extracellular vesicles counteract the immunosuppressive effect of melanoma-derived extracellular vesicles by Darshak K. Bhatt, Annemarie Boerma, Silvina Odete Bustos, Andréia Hanada Otake, Alexis Germán Murillo Carrasco, Patrícia Pintor Reis, Roger Chammas, Toos Daemen, Luciana Nogueira de Sousa Andrade

“…However, the impact of oncolytic viruses on the cargo and function of EVs released by cancer cells remains unknown. Here we show that upon oncolytic virotherapy with Semliki Forest virus-based replicon particles (rSFV), metastatic melanoma cells release EVs with a distinct biochemical profile and do not lead to suppression of immune cells. …”
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Audit and feedback is an effective implementation strategy to increase fidelity to a multi-component labor induction protocol designed to reduce obstetric inequities by Rebecca F. Hamm, Sreya Pattipati, Lisa D. Levine, Samuel Parry, Sindhu K. Srinivas, Rinad S. Beidas

“…To target identified implementation barriers, audit and feedback (A&F) was selected as an implementation strategy. Here, we aimed to understand the acceptability and effect of A&F on fidelity to this complex intervention through quantitative and qualitative approaches. …”
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A prospective, multi-site, cohort study to estimate incidence of infection and disease due to Lassa fever virus in West African countries (the Enable Lassa research programme)-Stud... by Suzanne Penfold, Ayola Akim Adegnika, Danny Asogun, Olufemi Ayodeji, Benedict N Azuogu, William A Fischer, Robert F Garry, Donald Samuel Grant, Christian Happi, Magassouba N'Faly, Adebola Olayinka, Robert Samuels, Jefferson Sibley, David A Wohl, Manfred Accrombessi, Ifedayo Adetifa, Giuditta Annibaldis, Anton Camacho, Chioma Dan-Nwafor, Akpénè Ruth Esperencia Deha, Jean DeMarco, Sophie Duraffour, Augustine Goba, Rebecca Grais, Stephan Günther, Énagnon Junior Juvénal Prince Honvou, Chikwe Ihekweazu, Christine Jacobsen, Lansana Kanneh, Mambu Momoh, Aminata Ndiaye, Robert Nsaibirni, Sylvanus Okogbenin, Chinwe Ochu, Ephraim Ogbaini, Énagnon Parsifal Marie Alexandre Logbo, John Demby Sandi, John S Schieffelin, Thomas Verstraeten, Nathalie J Vielle, Anges Yadouleton, Emmanuel Koffi Yovo, Enable Protocol authorship group

“…The core protocol described here harmonises key study components, such as eligibility criteria, case definitions, outcome measures, and laboratory tests, which will maximise the comparability of data for between-country analyses.…”
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Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis by Meimei Liu, Zhichao Wang, Hongwei Ni, Wenqing Zhuo, Gaoyuan Yuan, Zhiguo Dong

“…However, the impact of MHWs on Cyclina sinensis remains largely unknown. Here, this study assessed the effects of acute (25°C or 35°C for 12 h) and prolonged heatwave stress (no heatwave (25°C for 4 weeks), short heatwave (25°C for 3 weeks then 1 week at 35°C), and long heatwave (35°C for 4 weeks)) on the physiology and metabolism of C. sinensis. …”
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Enabling regulatory policy globally will promote realization of the potential of animal biotechnology by Eric Hallerman, Justin Bredlau, Luiz Sergio A. Camargo, Maria Lucia Zaidan Dagli, Margaret Karembu, Daniel Kovich, Anne Ndanu Muia, Mariana L. Murrone, Pedro Jesus Rocha-Salavarrieta, Rhodora Romero-Aldemita, Mark Tizard, Mark Walton, Diane Wray-Cahen

“…An international workshop on regulatory approaches for animal biotechnology was convened in 2022 with 27 countries represented. We synthesize here technical progress, development of regulatory policy, and strategies for engagement with diverse publics on animal biotechnology reported in the workshop. …”
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Investigating misclassification of type 1 diabetes in a population-based cohort of British Pakistanis and Bangladeshis using polygenic risk scores by Timing Liu, Alagu Sankareswaran, Gordon Paterson, Genes & Health Research Team, Diane P. Fraser, Sam Hodgson, Qin Qin Huang, Teng Hiang Heng, Meera Ladwa, Nick Thomas, David A. van Heel, Michael N. Weedon, Chittaranjan S. Yajnik, Richard A. Oram, Giriraj R. Chandak, Hilary C. Martin, Sarah Finer

“…Polygenic risk scores (PRSs) for T1D and T2D have been shown to work relatively well in South Asians, despite being derived from largely European-ancestry samples. Here we used PRSs to investigate the rate of potential misclassification of diabetes amongst British Bangladeshis and Pakistanis. …”
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Altered cytoskeleton dynamics in patient-derived iPSC-based model of PCDH19 clustering epilepsy by Rossella Borghi, Stefania Petrini, Valentina Apollonio, Marina Trivisano, Nicola Specchio, Sandra Moreno, Enrico Bertini, Marco Tartaglia, Claudia Compagnucci

“…Our previous work showed that PCDH19 dysfunction is associated with altered orientation of the mitotic spindle and accelerated neurogenesis, suggesting a contribution of altered cytoskeleton organization in PCDH19-CE pathogenesis in the control of cell division and differentiation. Here, we evaluate the consequences of altered PCDH19 function on microfilaments and microtubules organization, using a disease model obtained from patient-derived induced pluripotent stem cells. …”
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Antimicrobial Susceptibility of <i>Glaesserella parasuis</i> to Macrolides and Characterization of <i>erm</i>(T)-Carrying Mobile Elements on Chromosome by Peng Zhang, Changmin Li, Shuna Shang, Ting Huang, Junqi Liu, Qianwen Ge, Xiaoping Liao, Liangxing Fang, Yang Yu

“…Macrolide resistance poses an urgent threat to their treatment, as macrolides are widely used for preventing and treating <i>G. parasuis</i> infections. Here, we determined the susceptibilities to five macrolides and characterized the genetic markers of macrolide resistance. …”
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Repetitive Mild but Not Single Moderate Brain Trauma Is Associated with TAR DNA-Binding Protein 43 Mislocalization and Glial Activation in the Mouse Spinal Cord by Tamara Janković, Jelena Rajič Bumber, Nika Gržeta Krpan, Petra Dolenec, Marc Jaeger, Jasna Kriz, Gordana Župan, Kristina Pilipović

“…For both types of injury, sham-injured mice were used as a control group. <b>Results</b>: Here, we found an early formation of toxic phosphorylated TDP-43 species on the 3rdday post-injury which, together with TDP-43 cytoplasmic translocation, remained present in the subacute period of 14 days after repetitive mild but not single moderate TBI. …”
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