Context effects on repair of 5′‐overhang DNA double‐strand breaks induced by Cas12a in Arabidopsis by Sébastien Lageix, Miguel Hernandez, Maria E. Gallego, Jérémy Verbeke, Yannick Bidet, Sandrine Viala, Charles I. White

“…Abstract Sequence‐specific endonucleases have been key to the study of the mechanisms and control of DNA double‐strand break (DSB) repair and recombination, and the availability of CRISPR‐Cas nucleases over the last decade has driven rapid progress in the understanding and application of targeted recombination in many organisms, including plants. We present here an analysis of recombination at targeted chromosomal 5′ overhang DSB generated by the FnCas12a endonuclease in the plant, Arabidopsis thaliana. …”
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Thermally tunable add-drop filter based on valley photonic crystals for optical communications by Sun Lu, Li Xingfeng, Hu Pan, Wang Hongwei, Zhang Yong, Tang Guojing, He Xintao, Dong Jianwen, Su Yikai

“…However, there are few studies on how to achieve power-efficient tunable devices based on VPCs, which are essential for implementing basic functions such as optical switching and routing. Here, we propose and experimentally demonstrate a thermally tunable add-drop filter (ADF) based on VPCs operating at telecommunication wavelengths. …”
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Maximum carbon uptake potential through progressive management of plantation forests in Guangdong Province, China by Xueyan Li, Chongyuan Bi, Jianping Wu, Chaoqun Zhang, Wenting Yan, Zhenzhen Xiao, Ying-Ping Wang, Jiashun Ren, Yongxian Su

“…Yet given the declining carbon accumulation rates as forests mature and limited land for further planting, how best to manage these forests to maximize their future carbon uptake remains poorly studied. Here we employed fine-resolution satellite data, forest growth models, and machine learning to identify key drivers of carbon accumulation. …”
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Patients’ and plastic surgeons’ experiences with an online patient decision aid for breast reconstruction: considerations for nationwide implementation by Jacqueline A. ter Stege, Ellen G. Engelhardt, Leonie A.E. Woerdeman, Hester S.A. Oldenburg, Jacobien M. Kieffer, Daniela E.E. Hahn, Frederieke H. van Duijnhoven, Martine A. van Huizum, Regina The, Klemens Karssen, Marianne Kuenen, Miranda A. Gerritsma, Quinten (PQ) Ruhe, Irene S. Krabbe-Timmerman, Martijne van’t Riet, Nikola (AN) Kimmings, Eveline M.L. Corten, Kerry A. Sherman, Arjen J. Witkamp, Eveline M.A. Bleiker

“…We developed a patient decision aid (pDA) to support patients’ BR decision-making. Here, we assess patients’ and physicians’ use of the BR pDA and their views on the barriers and facilitators for widespread implementation. …”
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Anti-Scar Effects of Micropatterned Hydrogel after Glaucoma Drainage Device Implantation by Yiling Han, Qiangwang Geng, Aimeng Dong, Menglu Jiang, Jingyi Ma, Wulian Song, Pan Fan, Yuanyuan Li, Jiawen Gao, Fenghua Zhang, Jinsong Leng, Huiping Yuan

“…Although it is known that in implanted medical devices, microscale modification of the implant surface can modulate cell behavior and reduce the incidence of fibrosis, in the field of ophthalmic implants, especially the modification and effects of hydrogel micropatterns have rarely been reported. Here, we designed the patterned gelatin/acrylamide double network hydrogel and developed an innovative GDD with micropattern to suppress inflammatory and fibroblast activation after GDD implantation. …”
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Microbiota Landscape of Gut System of Guppy Fish (Poecilia reticulata) Plays an Outstanding Role in Adaptation Mechanisms by Christian Aimé Kayath, Armel Ibala Zamba, Joseph Goma-Tchimbakala, Victor Mamonékéné, Gloire Moniceth Mombo Makanga, Aimé Augustin Lebonguy, Etienne Nguimbi

“…Microbial consortium that is present in fish gut systems works together to achieve unknown specific roles. Here, we collected guppy fish from hydrocarbon- and trace metal-contaminated wastewater to assess the relationships between gut microbiota and host fish adaptation. …”
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A Neural Circuit For Bergamot Essential Oil‐Induced Anxiolytic Effects by Meng‐Yu Zhu, Wan‐Ying Dong, Jin‐Rong Guo, Ji‐Ye Huang, Ping‐Kai Cheng, Yumeng Yang, An Liu, Xin‐Lu Yang, Xia Zhu, Zhi Zhang, Yuanyin Wang, Wenjuan Tao

“…Abstract Aromatic essential oils have been shown to relieve anxiety and enhance relaxation, although the neural circuits underlying these effects have remained unknown. Here, it is found that treatment with 1.0% bergamot essential oil (BEO) exerts anxiolytic‐like effects through a neural circuit projecting from the anterior olfactory nucleus (AON) to the anterior cingulate cortex (ACC) in acute restraint stress model mice. …”
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On the time lag between sea-level rise and basin infilling at tidal inlets by Roshanka Ranasinghe, Zheng Bing Wang, Janaka Bamunawala, Trang Minh Duong

“…To date, an indicative M value has only been derived for small tidal inlet systems (M ~0.5), and due to the lack of M estimates for larger systems, studies have been using M ~0.5 indiscriminately. Here, for the first time, we derive indicative M values for small, medium, and large tidal inlet systems (M ~0.5, ~0.25 and ~0.15 respectively) via analytical considerations. …”
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Phylogeography and genetic diversity of Ulmus elongata (Ulmaceae), a Tertiary relict tree with extremely small populations (PSESP) by Yakun Wang, Xiankun Wang, Junyuan Wu, Jun Yang, Yanpei Liu, Peng Guo, Fude Shang, Nan Lin

“…Therefore, a further study on the genetic diversity and drivers of genetic pattern in U. elongata is crucial for preserving genetic resources and can serve as a reference for other Tertiary relict plants and PSESP under climate change. Results Here, a total of 12 populations from 70 individuals of U. elongata were collected covering its geographical distribution in China. …”
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High and low dose radiotherapy combined with ICIs for MSS colorectal cancer patients with liver metastases: a phase I study (HaRyPOT) by Yuxuan Ding, Yong Feng, Yangfan Ye, Jiayi Shen, Chang Guo, Xia He, Liangjun Zhu, Lijun Wang

“…Radiotherapy in combination with immune checkpoint inhibitors is one of promising therapies, and the choice of radiotherapy and immunotherapy modalities is also a hot issue.MethodsHere, we report on a Phase I trial treating nine patients with MSS CLRM using a combination of high and low dose radiotherapy and immune checkpoint inhibitors (ICIs).ResultsThe primary endpoint of the trial was the safety and tolerability of this combination treatment modality. …”
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Long-term outcome of photodynamic therapy with hexyl aminolevulinate, 5-aminolevulinic acid nanoemulsion and methyl aminolevulinate for low-risk Basal Cell Carcinomas by M. Salmivuori, M. Grönroos, T. Tani, J. Räsänen, E. Snellman, N. Neittaanmäki

“…We have previously reported comparable short-term efficacies for MAL 16 %, BF-200 ALA 7.8 % and HAL 2 % in PDT of low-risk BCC, and here we report the long-term results. Objectives: The goal of this trial was to compare long-term outcomes of HAL and BF-200 ALA, compared to MAL in PDT of low-risk BCCs. …”
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Thyroid hormone receptor- and stage-dependent transcriptome changes affect the initial period of Xenopus tropicalis tail regeneration by Shouhong Wang, Liezhen Fu, Bin Wang, Yanmei Cai, Jianping Jiang, Yun-Bo Shi

“…It is well established that the developmental effects of T3 are primarily mediated through transcriptional regulation by thyroid hormone receptors (TRs). Here we have taken advantage of mutant tadpoles lacking both TRα and TRβ (TRDKO), the only receptor genes in vertebrates, for RNA-seq analyses to investigate the transcriptome changes underlying the initiation of tail regeneration, i.e., wound healing and blastema formation, because this crucial initial step determines the extent of the functional regeneration in the later phase of tissue regrowth. …”
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Top health service concerns: a data mining study of the Shanghai health hotline by Lili Shi, Tong Zhao, Shimiao Shi, Tianyu Tan, Aksara Regmi, Yuyang Cai

“…The methodologies employed here can be applied to other urban health contexts, providing a valuable framework for improving public health strategies globally.…”
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Impact of wave–current coupling on the bottom boundary layer in Bohai Bay by Siyu Liu, Siyu Liu, Guangxue Li, Xue Liu, Lulu Qiao, Nan Wang, Shidong Liu, Xiangdong Wang, Di Yu, Lei Zhang

“…Our results highlight the profound impacts of wave–current interactions on the formation and disappearance of the FML within the BBL in silty, shallow marine environments. The work here offers critical insights into the dynamics of fine-particle sediment, and provides suggestions for mitigating the negative effects associated with the FML.…”
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Targeting murine metastatic cancers with cholera toxin A1-adjuvanted peptide vaccines by Sanchari Paul, Mustafa Kaya, Olivia Johnsson, Hanna Grauers Wiktorin, Andreas Törnell, Mohammad Arabpour, Kristoffer Hellstrand, Anna Martner

“…Cholera toxin-based adjuvants have shown efficacy in vaccines for infectious diseases, but their role in cancer vaccine therapies remains to be elucidated. Here, we explored the potential of cholera toxin A1 (CTA1)-based adjuvants to boost anti-tumor T cell responses and protect against metastasis. …”
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Blobby is a synaptic active zone assembly protein required for memory in Drosophila by J. Lützkendorf, T. Matkovic-Rachid, S. Liu, T. Götz, L. Gao, O. Turrel, M. Maglione, M. Grieger, S. Putignano, N. Ramesh, T. Ghelani, A. Neumann, N. Gimber, J. Schmoranzer, A. Stawrakakis, B. Brence, D. Baum, Kai Ludwig, M. Heine, T. Mielke, F. Liu, A. M. Walter, M. C. Wahl, S. J. Sigrist

“…However, regulatory principles steering AZ scaffold assembly, function, and plasticity remain insufficiently understood. We here identify an additional Drosophila AZ protein, “Blobby”, essential for proper AZ nano-organization. …”
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Balancing on carbon storage and ecological security in urban areas: The case of Dongguan city, China by Xinyi Wang, Fenzhen Su, Fengqin Yan, Vincent Lyne, Yikun Cui, Bin He, Rong Fan

“…Current and future national urban policies must therefore enhance carbon sink functions of ecosystems while maintaining spatial ecological structure networks. Here, we assessed current policies against future scenarios using systems thinking and land-use change simulation coupled to carbon-storage accounting. …”
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The Campylobacter jejuni BumS sensor phosphatase detects the branched short-chain fatty acids isobutyrate and isovalerate as direct cues for signal transduction by Nestor Ruiz, Jiawei Xing, Igor B. Zhulin, Chad A. Brautigam, David R. Hendrixson

“…However, BumS only indirectly senses butyrate to inhibit dephosphorylation of its cognate BumR response regulator. Here, we expanded the repertoire of cues sensed by BumS to the branched-short chain fatty acids isobutyrate and isovalerate that are also abundant in the lower intestines. …”
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Atherogenic Index of Plasma (AIP) a Tool to Assess Changes in Cardiovascular Disease Risk Post Laparoscopic Sleeve Gastrectomy by Eman Al Shawaf, Ebaa Al-Ozairi, Fahad Al-Asfar, Anwar Mohammad, Shaima Al-Beloushi, Sriraman Devarajan, Fahd Al-Mulla, Jehad Abubaker, Hossein Arefanian

“…However, studies utilizing predictive indices for CVD risk in CVD-free morbid-obese patients who undertook laparoscopic sleeve gastrectomy (LSG) are lacking. Here, we use AIP as a tool to evaluate the improvement in CVD risk post-LSG in morbid-obese people who had no history of CVD. …”
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A human skin-on-a-chip platform for microneedling-driven skin cancer treatment by Natan R. Barros, Raehui Kang, Jinjoo Kim, Menekse Ermis, Han-Jun Kim, Mehmet R. Dokmeci, Junmin Lee

“…However, challenges remain in creating models suitable for microneedling applications and monitoring, as well as developing skin cancer models for analysis and targeted therapy. Here, we developed a human skin/skin cancer-on-a-chip platform within a microfluidic device using bioprinting/bioengineering techniques. …”
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