A guide to selecting high-performing antibodies for Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PPP2R5D) for use in Western Blot, immunoprecipi... by Carl Laflamme, Kathleen Southern, Riham Ayoubi, Maryam Fotouhi, Charles Alende

“…The etiology of these genetic disorders remains unknown, which can partly be due to the lack of independently characterized antibodies. Here we have characterized six PPP2R5D commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. …”
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Level of physical activity of students from the police force academy in Bratislava by Gabriel Bujdos, Štefan Adamčák, Michal Marko, Anna Kozaňáková

“…Female participants of our study were slightly more active in walking physical activity, 2.92 minutes a week on average, but the difference between our two studied groups have not proven to be statistically significant at the level of significance p < 0.05. Conclusions. Here in our study, we’ve looked at physical activity, physical activity levels and how much students attending Academy of the Police Force in Bratislava engage in them. …”
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Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis by Fábio Peixoto, Maurício T. Nascimento, Rúbia Costa, Juliana Silva, Gaby Renard, Luiz Henrique Guimarães, Gerson Penna, Manoel Barral-Netto, Lucas P. Carvalho, Paulo R. L. Machado, Edgar M. Carvalho

“…Miltefosine is more effective than pentavalent antimony (Sbv) in the treatment of CL, and here, we evaluate the ability of Sbv, miltefosine, and GM-CSF administered intravenously, orally, or topically, respectively, to modify the immune response. …”
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ŽINOJIMO SOCIALUMO PROBLEMA ŠIUOLAIKINĖJE SOCIALINĖJE EPISTEMOLOGIJOJE: DU POŽIŪRIAI by Audronė Rimkutė

“…Knowledge is conceived here as institutionalised belief, and epistemology as defined as knowledge economics and as science policy. …”
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Toward super-clean bearing steel by a novel physical-data integrated design strategy by Jian Guan, Guolei Liu, Wenguang Hu, Hongwei Liu, Paixian Fu, Yanfei Cao, Dong-Rong Liu, Dianzhong Li

“…Finally, a systematic framework based on a genetic algorithm was proposed to select the optimal combination of CAS. Here, the ML-optimized processing parameters comprised current of 4255 A, helium pressure of 0.69 kPa, and melting rate of 2.5 kg/min. …”
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The subventricular zone neurogenic niche provides adult born functional neurons to repair cortical brain injuries in response to diterpenoid therapy by Ricardo Pardillo-Díaz, Patricia Pérez-García, María Ortego-Domínguez, Ricardo Gómez-Oliva, Nora Martínez-Gómez, Samuel Domínguez-García, Francisco García-Cózar, Juan Pedro Muñoz-Miranda, Rosario Hernández-Galán, Livia Carrascal, Carmen Castro, Pedro Nunez-Abades

“…Providing the injured area with the adequate signaling molecules may improve both migration and functional integration of newly generated neurons. Methods In here, we have studied the effect of a diterpene with the capacity to induce neuregulin release at promoting neurogenesis in a murine model of cortical brain injury. …”
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Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens by Daqing Dai, Xiqiong Wang, Kexin Wu, Fangren Lan, Jiaming Jin, Wenxin Zhang, Chaoliang Wen, Junying Li, Ning Yang, Congjiao Sun

“…Simultaneously, protein modification is affected by the surrounding environment, which ultimately affects the structure and function of proteins. Here, we measured the phenotypes of eggs at different time points during storage and incubation and used 4D label-free quantitative proteomics technology and liquid chromatography/tandem mass spectrometry (LC-MS/MS)-technique to identify the differential proteins and N-glycosylation sites in egg whites during storage and incubation. …”
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Allogeneic DNT cell therapy synergizes with T cells to promote anti-leukemic activities while suppressing GvHD by Jongbok Lee, Hyeonjeong Kang, Branson Chen, Yoosu Na, Ismat Khatri, Fraser Soares, Housheng Hansen He, Arjun D. Law, Tianzhong Pan, Armin Gerbitz, Xiaoyu Zhu, Mark D. Minden, Li Zhang

“…A phase I clinical trial demonstrated the feasibility, safety, and potential efficacy of allogeneic DNT therapy for patients with relapsing acute myeloid leukemia (AML) post-allo-HSCT. Here, we studied the impact of DNTs on the anti-leukemic and GvHD-inducing activities of Tconv cells. …”
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Cross-protection against homo and heterologous influenza viruses via intranasal administration of an HA chimeric multiepitope nanoparticle vaccine by Yongqiang Zhao, Jia Liu, Chun Peng, Shuangshuang Guo, Bo Wang, Longping Chen, Yating Wang, Haiwen Tang, Liming Liu, Qi Pan, Shiren Li, Jingyu Wang, Dongni Yang, Enqi Du

“…Multiepitope vaccines combining multiple conserved epitopes can induce more robust and broader immune responses and provide a potential solution. Results Here, we demonstrated that an HA chimeric multiepitope nanoparticle vaccine, delivered intranasally conferred broad protection against challenges with various influenza viruses in mice. …”
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Chemical profiles of venom glands in queens, foundresses, pre-wintering gynes, and workers in the hornet Vespa velutina nigrithorax by Berville, Laurence, Lucas, Christophe, Haouzi, Mélissa, Khalil, Ali, Gévar, Jérémy, Bagnères, Anne-Geneviève, Darrouzet, Eric

“…Naturally produced by insects, semio-chemicals have been proposed as integrated management tools in this context, either for disruption or mass trapping, as an alternative tool to conventional non-selective traps. Here, we focused on the venom gland, which produces the alarm pheromone. …”
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Multiphoton imaging-based quantifiable collagen signatures for predicting outcomes in patients with pancreatic ductal adenocarcinoma by Xiwen Chen, Jikui Miao, Xingxin Huang, Xiahui Han, Liqin Zheng, Jianxin Chen, Linying Chen, Lianhuang Li

“…Abstract Pancreatic ductal adenocarcinoma (PDAC) presents a clinical challenge due to its poor prognosis and high mortality rate. Here, we aimed to enhance the prognostic prediction of patients with PDAC by studying collagen features in tumor microenvironment using multiphoton microscopy (MPM) combining with image processing technique. …”
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GluN2D-containing NMDA receptors in parvalbumin neurons in the nucleus accumbens regulate nocifensive responses in neuropathic pain by Sukanya G. Gakare, Gajanan P. Shelkar, Dinesh Y. Gawande, Ratnamala Pavuluri, Pauravi J. Gandhi, Shashank M. Dravid

“…Neuropathic pain presents a significant challenge, with its underlying mechanisms still not fully understood. Here, we investigated the role of GluN2C- and GluN2D-containing NMDA receptors in the development of neuropathic pain induced by cisplatin, a widely used chemotherapeutic agent. …”
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Evidence for direct dopaminergic connections between substantia nigra pars compacta and thalamus in young healthy humans by Giovanni Cirillo, Giuseppina Caiazzo, Federica Franza, Mario Cirillo, Michele Papa, Fabrizio Esposito

“…The impossibility of tract-tracing studies in humans has boosted advanced MRI techniques and multi-shell high-angular resolution diffusion MRI (MS-HARDI) has promised to shed more light on the structural connectivity of subcortical structures. Here, we estimated the possible dopaminergic innervation of the human thalamus via an MS-HARDI tractography of the SNc in healthy human young adults. …”
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Hotspots of genetic change in Yersinia pestis by Yarong Wu, Youquan Xin, Xiaoyan Yang, Kai Song, Qingwen Zhang, Haihong Zhao, Cunxiang Li, Yong Jin, Yan Guo, Yafang Tan, Yajun Song, Huaiyu Tian, Zhizhen Qi, Ruifu Yang, Yujun Cui

“…Abstract The relative contributions of mutation rate variation, selection, and recombination in shaping genomic variation in bacterial populations remain poorly understood. Here we analyze 3318 Yersinia pestis genomes, spanning nearly a century and including 2336 newly sequenced strains, to shed light on the patterns of genetic diversity and variation distribution at the population level. …”
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Effectiveness of an Innovative Pulsed Electromagnetic Fields Stimulation in Healing of Untreatable Skin Ulcers in the Frail Elderly: Two Case Reports by Fabio Guerriero, Emanuele Botarelli, Gianni Mele, Lorenzo Polo, Daniele Zoncu, Paolo Renati, Carmelo Sgarlata, Marco Rollone, Giovannoi Ricevuti, Niccolò Maurizi, Matthew Francis, Mariangela Rondanelli, Simone Perna, Davide Guido, Piero Mannu

“…Pulsed electromagnetic fields (PEMFs) have proved to be a promising therapy for wound healing. Here we describe the first reported case of an innovative PEMF therapy, Emysimmetric Bilateral Stimulation (EBS), used to successfully treat refractory skin ulcers in two elderly and fragile patients. …”
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Quantum key distribution implemented with d-level time-bin entangled photons by Hao Yu, Stefania Sciara, Mario Chemnitz, Nicola Montaut, Benjamin Crockett, Bennet Fischer, Robin Helsten, Benjamin Wetzel, Thorsten A. Goebel, Ria G. Krämer, Brent E. Little, Sai T. Chu, Stefan Nolte, Zhiming Wang, José Azaña, William J. Munro, David J. Moss, Roberto Morandotti

“…As well, increasing the number of time bins per photon state typically requires decreasing the repetition rate of the system, affecting in turn the effective qudit rates. Here, we demonstrate a fiber-pigtailed, integrated photonic platform enabling the generation and processing of picosecond-spaced time-bin entangled qudits in the telecommunication C band via an on-chip interferometry system. …”
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Constraining the Geochemical Fingerprints of Gases from the UK Carboniferous Coal Measures at the Glasgow Geoenergy Observatories Field Site, Scotland by Rebecca M. Chambers, Gareth Johnson, Adrian J. Boyce, Stuart M. V. Gilfillan

“…Determining the presence, magnitude, and origin of subsurface gases, and how their geochemical fingerprints evolve within the shallow subsurface is vital to developing an understanding of how to manage the risk posed by ground gases in geoenergy technology development. Here, we present the first CH4 and CO2 concentration-depth profiles and stable isotope (δ13CCH4, δ13CCO2, and δDCH4) profiles obtained from UK mine workings, through analysis of headspace gas samples degassed from cores and chippings collected during construction of the Glasgow Observatory. …”
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Roads to remission: evolving treatment concepts in type 2 inflammatory diseases by Marek Lommatzsch, Katharina Blumchen, Lisa A. Beck, Jean Bousquet, Guy G. Brusselle, Wytske J. Fokkens, Eckard Hamelmann, Susanne Lau, Hagen Ott, Oliver Pfaar, Hugh A. Sampson, Josef S. Smolen, Christian Taube, Ingo H. Tarner, Martin Wagenmann, Thomas Werfel, Margitta Worm, Harald Renz

“…This new paradigm has been developed based on a better understanding of the pathophysiology of type 2 inflammation and the advent of highly effective drugs selectively interfering with type 2 pathways. Here, we review the evolution of the new remission concepts in type 2 inflammatory diseases and discuss associated challenges and future research needs. …”
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Single-cell atlas of human pancreatic islet and acinar endothelial cells in health and diabetes by Rebecca Craig-Schapiro, Ge Li, Kevin Chen, Jesus M. Gomez-Salinero, Ryan Nachman, Aleksandra Kopacz, Ryan Schreiner, Xiaojuan Chen, Qiao Zhou, Shahin Rafii, David Redmond

“…Abstract Characterization of the vascular heterogeneity within the pancreas has previously been lacking. Here, we develop strategies to enrich islet-specific endothelial cells (ISECs) and acinar-specific endothelial cells (ASECs) from three human pancreases and corroborate these findings with three published pancreatic datasets. …”
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Layer-by-layer phase transformation in Ti3O5 revealed by machine-learning molecular dynamics simulations by Mingfeng Liu, Jiantao Wang, Junwei Hu, Peitao Liu, Haiyang Niu, Xuexi Yan, Jiangxu Li, Haile Yan, Bo Yang, Yan Sun, Chunlin Chen, Georg Kresse, Liang Zuo, Xing-Qiu Chen

“…Despite extensive studies, the underlying microscopic mechanism remains unclear. Here, we discover a kinetically favorable in-plane nucleated layer-by-layer transformation mechanism through metadynamics and large-scale molecular dynamics simulations. …”
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