Mapping Nanoscale‐To‐Single‐Cell Phosphoproteomic Landscape by Chip‐DIA by Gul Muneer, Sofani Tafesse Gebreyesus, Ciao‐Syuan Chen, Tzu‐Tsung Lee, Fengchao Yu, Chih‐An Lin, Min‐Shu Hsieh, Alexey I. Nesvizhskii, Chao‐Chi Ho, Sung‐Liang Yu, Hsiung‐Lin Tu, Yu‐Ju Chen

“…However, it remains a formidable analytical challenge due to the low detectability, abundance, and stoichiometry of phosphorylation sites. Here, we present a Chip‐DIA strategy, integrating a microfluidic‐based phosphoproteomic chip (iPhosChip) with data‐independent acquisition mass spectrometry (DIA‐MS) for ultrasensitive nanoscale‐to‐single‐cell phosphoproteomic profiling. …”
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Transforming Triple Cropping System to Four Crops Pattern: An Approach of Enhancing System Productivity through Intensifying Land Use System in Bangladesh by Md. Aminul Islam, Md. Jahedul Islam, M. Akkas Ali, A. S. M. Mahbubur Rahman Khan, Md. Faruque Hossain, Md. Moniruzzaman

“…Aman rice. Maize was introduced here as a relay crop with onion to fit it in the four crops pattern. …”
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Climate change as a wildlife health threat: a scoping review by Sabrina S. Greening, Lucie R. Pascarosa, Avery L. Munster, Roderick B. Gagne, Julie C. Ellis

“…Practitioners are working to integrate concepts such as vulnerability, adaptation, and resilience into wildlife health research, surveillance, and management actions. Here, we performed a scoping review to identify scholarly articles from 2008 onwards with a focus on climate change impacts on wildlife health. …”
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Spatial transcriptomics unveils estrogen-modulated immune responses and structural alterations in the ectocervical mucosa of depot medroxyprogesterone acetate users by Vilde Kaldhusdal, Mathias Franzen Boger, Annelie Tjernlund, Adam D. Burgener, Frideborg Bradley, Julie Lajoie, Kenneth Omollo, Joshua Kimani, Keith Fowke, Paulo Czarnewski, Kristina Broliden

“…High-resolution spatial transcriptomics is applied here to reveal the spatial localization of these altered molecular markers. …”
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Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs by Euler Moraes Penha, Cássio Santana Meira, Elisalva Teixeira Guimarães, Marcus Vinícius Pinheiro Mendonça, Faye Alice Gravely, Cláudia Maria Bahia Pinheiro, Taiana Maria Bahia Pinheiro, Stella Maria Barrouin-Melo, Ricardo Ribeiro-dos-Santos, Milena Botelho Pereira Soares

“…The use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. …”
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Molecular correlates for HPV-negative head and neck cancer engraftment prognosticate patient outcomes by Matthew Waas, Christina Karamboulas, Benson Z. Wu, Shahbaz Khan, Stephanie Poon, Jalna Meens, Meinusha Govindarajan, Amanda Khoo, Salvador Mejia-Guerrero, Annie Ha, Lydia Y. Liu, Kevin C. J. Nixon, Joseph Walton, Scott V. Bratman, Shao Hui Huang, David Goldstein, Federico Gaiti, Laurie Ailles, Thomas Kislinger

“…However, assessing engraftment is challenging in clinical settings. Here, we perform transcriptomic and proteomic profiling of 88 HNSCC patients and find the relationship between engraftment and clinical outcomes is recapitulated by molecular phenotype. …”
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Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion by Elisa Montanari, Joel Pimenta, Luca Szabó, François Noverraz, Solène Passemard, Raphael P. H. Meier, Jeremy Meyer, Jonathan Sidibe, Aurelien Thomas, Henk-Jan Schuurman, Sandrine Gerber-Lemaire, Carmen Gonelle-Gispert, Leo H. Buhler

“…Porcine hepatocytes transplanted during acute liver failure might support metabolic functions until the diseased liver recovers its function. Here, we isolated high numbers of viable pig hepatocytes and evaluated hepatocyte functionality after encapsulation. …”
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A Body‐Temperature‐Triggered In Situ Softening Peripheral Nerve Electrode for Chronic Robust Neuromodulation by Xueyang Ren, Wenjie Tang, Yuehui Yuan, Shisheng Chen, Fangzhou Lu, Jinyang Mao, Jidan Fan, Xufeng Wei, Ming Chu, Benhui Hu

“…However, these mechanically tissue‐like electrodes suffer from unmanageable operating forces, leading to high risks of nerve injury and fragile electrode‐tissue interfaces. Here, a peripheral nerve electrode is developed that simultaneously fulfills the criteria of body temperature softening and tissue‐like modulus (less than 0.8 MPa at 37 °C) after implantation. …”
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Insecticide resistant Anopheles from Ethiopia but not Burkina Faso show a microbiota composition shift upon insecticide exposure by Netsanet Worku, Antoine Sanou, Juliane Hartke, Marion Morris, Fatoumata Cissé, Salimata Ouédraogo, Madou Tapsoba, Nicola Vallon, Tewodros Debebe Akilu, Ligabaw Worku, Moussa Wamdaogo Guelbeogo, Victoria A. Ingham

“…Understanding the underlying mechanisms contributing to this phenotype is necessary to both track the spread of resistance and to design new tools to overcome it. Methods Here, we compare the microbiota composition of insecticide-resistant populations of Anopheles gambiae, An. coluzzii and An. arabiensis from Burkina Faso, and in the latter case additionally from Ethiopia, to insecticide-susceptible populations. …”
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Colder temperatures augment viability of an indirectly transmitted songbird pathogen on bird feeders by Sara R. Teemer, Edan R. Tulman, Alicia G. Arneson, Steven J. Geary, Dana M. Hawley

“…Although bird feeder surfaces serve as an indirect route of MG transmission between sick and healthy individuals, the contributions of feeders to MG transmission in the wild will depend on how ambient temperature affects viability and pathogenicity of MG on feeder surfaces over time. Here, we used two experiments, with identical initial design, to assess such temperature effects. …”
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A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula by Nicolás Vidal-Vázquez, Ismael Hernández-Núñez, Pablo Carballo-Pacoret, Sarah Salisbury, Paula R. Villamayor, Francisca Hervas-Sotomayor, Xuefei Yuan, Francesco Lamanna, Céline Schneider, Julia Schmidt, Sylvie Mazan, Henrik Kaessmann, Fátima Adrio, Diego Robledo, Antón Barreiro-Iglesias, Eva Candal

“…However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. …”
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Spatiotemporal Assessment of Temperature Data Products for the Detection of Warming Trends and Abrupt Transitions over the Largest Irrigated Area of Pakistan by Zain Nawaz, Xin Li, Yingying Chen, Xufeng Wang, Kun Zhang, Naima Nawaz, Yanlong Guo, Akynbekkyzy Meerzhan

“…Gridded data products (GDPs) offer an opportunity to estimate and monitor temperature indices at a range of spatiotemporal resolutions; however, their reliability must be quantified by spatiotemporal comparison against in situ records. Here, we present spatial and temporal assessments of temperature indices (Tmax, Tmin, Tmean, and DTR) products against the reference data during the period of 1979–2015 over Punjab Province, Pakistan. …”
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Genetic diversity of the immunoglobulin heavy chain locus in cohorts of patients affected with SARS-CoV-2 by Patrizia Malaspina, Carla Jodice, Bianca Maria Ciminelli, Michela Biancolella, Vito Luigi Colona, Andrea Latini, Francesca Leonardis, Paola Rogliani, Antonio Novelli, Giuseppe Novelli, Andrea Novelletto

“…HS1.2), which are flanked by the SNP set here analyzed.…”
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Middle East respiratory syndrome coronavirus (MERS-CoV) internalization does not rely on DPP4 cytoplasmic tail signaling by Karthika Thankamani, Divakar Shubham, Gayatri Kandpal, Ann Mary Isaac, Modenkattil Sethumadhavan Kavitha, V. Stalin Raj

“…However, the role of the cytoplasmic tail in the entry process remains unclear. Here, we show that mutating or deleting individual aa residues or the entire cytoplasmic tail of DPP4 (ΔcytDPP4) does not completely prevent DPP4 from being inserted into the membrane or from allowing the binding of the MERS-CoV spike protein and pseudovirus infection. …”
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Novel protein-based prognostic signature linked to immunotherapeutic efficiency in ovarian cancer by Shuo-Fu Chen, Liang-Yun Wang, Yi-Sian Lin, Cho-Yi Chen

“…While conventional clinicopathological factors have exhibited limited efficacy as prognostic indicators in ovarian cancer, multi-omics profiling presents a promising avenue for comprehending the interplay between the tumor and immune components. Here we aimed to leverage the individual proteomic and transcriptomic profiles of ovarian cancer patients to develop an effective protein-based signature capable of prognostication and distinguishing responses to immunotherapy. …”
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A novel cytoprotective organ perfusion platform for reconstructing homeostasis of DCD liver while alleviating IRI injury by Tingting Lan, Mingxing Yu, Tao Ming, Hong Wang, Juan Deng, Shuhan Cheng, Zhongyang Shen, Deling Kong

“…However, the continuous extrusion forming with the rigid stationary shaft of the peristaltic pumps can damage cells, leading to metabolic disorders and eventual dysfunction of transplanted organs. Here, we developed a novel biomimetic blood‐gas system (BBGs) for preventing cell damage. …”
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Design and structure of overlapping regions in PCA via deep learning by Yan Zheng, Xi-Chen Cui, Fei Guo, Ming-Liang Dou, Ze-Xiong Xie, Ying-Jin Yuan

“…However, there still exists DNA sequences that are challenging to design and construct in the genome synthesis. Here we proposed a deep learning model based on extensive synthesis data to discern latent sequence representations in overlapping regions with an AUPR of 0.805. …”
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Hydrogen sulfide attenuates disturbed flow-induced vascular remodeling by inhibiting LDHB-mediated autophagic flux by Xia Wang, Xiying Huang, Yongya Zhang, Huanhuan Huo, Guo Zhou, Linghong Shen, Long Li, Ben He

“…However, its specific contribution to DF-induced vascular remodeling remains unclear. Here, we showed that the H2S donor, NaHS suppressed DF-induced vascular remodeling in mice. …”
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CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments by Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su

“…Therefore, enhanced monitoring of NoV in aqueous environments plays an active role in preventing diseases. Here, we reverse transcribed viral RNA into cDNA, and then used the constructed RPA-CRISPR/Cas13a-based platform for sensitive and quantitative monitoring of NoV GII.4 in aqueous environments. …”
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Host Factors in the Natural History of Chronic Hepatitis B: Role of Genetic Determinants by null Turyadi, Billy Witanto, Korri E. El-Khobar, M. Luthfi Parewangi, M. Rezki Rasyak, Dhita P. Wibowo, Meta D. Thedja, Irawan Yusuf, Muh. Nasrum Massi, Ilhamjaya Patellongi, Din Syafruddin, David H. Muljono

“…CHB progression can be categorized into four phases, immune tolerance (IT), immune clearance (IC), low/no replicative (LR), and e-negative hepatitis (ENH), with HBeAg seroconversion as an important milestone. Here, we determined the association of TNF-α (rs1800629) and IL-10 (rs1800896 and rs1800872) SNPs in the context of CHB natural history progression, particularly to HBeAg seroconversion, in Indonesian CHB patients. …”
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