The Wayqecha Amazon Cloud Curtain Ecosystem Experiment: A new experimental method to manipulate fog water inputs in terrestrial systems by Daniel B. Metcalfe, Darcy F. Galiano Cabrera, Luis Miguel Alvarez Mayorga, Roxana Sacatuma Cruz, Daniela Corrales Alvarez, Blanca Rosa Espinoza Otazu, Walter Huaraca Huasco, Jimmy R. Chambi, Maria E. Ezquerra, Beisit L. Puma Vilca, Mark Mulligan, Matthew A. Vadeboncoeur, Heidi Asbjornsen, Paulo R. L. Bittencourt, Aline B. Horwath, David C. Bartholomew

“…The amount and frequency of fog immersion are affected by rapid ongoing anthropogenic changes but the impacts of these changes remain relatively poorly understood compared with changes in rainfall. Here, we present the design and performance of a novel experiment to actively manipulate low lying fog abundance in an old‐growth tropical montane cloud forest (TMCF) in Peru—the Wayqecha Amazon Cloud Curtain Ecosystem Experiment (WACCEE). …”
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Delineation of the Genetic Architecture and Clinical Polymorphism of 3q29 Duplication Syndrome: A Review of the Literature and a Report of Two Novel Patients With Single‐Gene BDH1... by A. A. Kashevarova, M. E. Lopatkina, O. Yu. Vasilyeva, D. A. Fedotov, A. D. Lobanov, E. A. Fonova, I. Z. Zhalsanova, A. A. Zarubin, O. A. Salyukova, E. O. Belyaeva, V. V. Petrova, E. G. Ravzhaeva, A. A. Agafonova, A. D. Cheremnykh, N. B. Torkhova, S. L. Vovk, I. N. Lebedev

“…Methods Patients were investigated by performing a cytogenetic analysis of GTG‐banded metaphases, aCGH with the SurePrint G3 Human CGH Microarray 8×60K, qPCR, FISH, and WES. Results Here, we report five new patients with atypical duplications overlapping with the 3q29 duplication syndrome region and no other genetic findings. …”
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The role of OCO-3 XCO₂ retrievals in estimating global terrestrial net ecosystem exchanges by X. Wang, F. Jiang, F. Jiang, F. Jiang, H. Wang, Z. Zhang, M. Wu, J. Wang, W. He, W. Ju, W. Ju, J. M. Chen, J. M. Chen

“…The Orbiting Carbon Observatory 3 (OCO-3) satellite, launched in May 2019, was designed to address important questions about the distribution of carbon fluxes on Earth, but its role in estimating global terrestrial NEE remains unclear. Here, using the Global Carbon Assimilation System, version 2, we investigate the impact of OCO-3 XCO<span class="inline-formula">₂</span> on the estimation of global NEE by assimilating the OCO-3 XCO<span class="inline-formula">₂</span> retrievals alone and in combination with the OCO-2 XCO<span class="inline-formula">₂</span> retrievals. …”
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Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target by Cèlia Dobaño-López, Juan García Valero, Ferran Araujo-Ayala, Ferran Nadeu, Fabien Gava, Carla Faria, Marine Norlund, Renaud Morin, Pascale Bernes-Lasserre, Fabian Arenas, Marta Grau, Cristina López, Irene López-Oreja, Neus Serrat, Ares Martínez-Farran, Lluís Hernández, Heribert Playa-Albinyana, Rubén Giménez, Silvia Beà, Elías Campo, Jean-Michel Lagarde, Armando López-Guillermo, Laura Magnano, Dolors Colomer, Christine Bezombes, Patricia Pérez-Galán

“…Abstract Follicular lymphoma (FL), the most common indolent non-Hodgkin lymphoma, constitutes a paradigm of immune tumor microenvironment (TME) contribution to disease onset, progression, and heterogenous clinical outcome. Here we present the first FL-Patient Derived Lymphoma Spheroid (FL-PDLS), including fundamental immune actors and features of TME in FL lymph nodes (LNs). …”
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Ocean equity: from assessment to action to improve social equity in ocean governance by Nathan J. Bennett, Nathan J. Bennett, Nathan J. Bennett, Nathan J. Bennett, Veronica Relano, Katina Roumbedakis, Katina Roumbedakis, Katina Roumbedakis, Jessica Blythe, Mark Andrachuk, Joachim Claudet, Neil Dawson, David Gill, Natali Lazzari, Natali Lazzari, Natali Lazzari, Shauna L. Mahajan, Ella-Kari Muhl, Maraja Riechers, Mia Strand, Sebastian Villasante

“…To date, however, there has been limited attention to how to meaningfully assess status and monitor progress on social equity in ocean governance (aka “ocean equity”) across different marine policy realms. Here, we contribute to ongoing efforts to advance ocean equity through providing guidance on five steps to develop bespoke, fit to purpose and contextually appropriate assessment and monitoring frameworks and approaches to measure status of and track changes in ocean equity. …”
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Mapping naturally presented T cell antigens in medulloblastoma based on integrative multi-omics by Julia Velz, Lena K. Freudenmann, Gioele Medici, Marissa Dubbelaar, Malte Mohme, David R. Ghasemi, Jonas Scheid, Daniel J. Kowalewski, Angelica B. Patterson, Anna M. Zeitlberger, Katrin Lamszus, Manfred Westphal, Matthias Eyrich, Martina Messing-Jünger, Andreas Röhrig, Harald Reinhard, Kévin Beccaria, Rogeiro B. Craveiro, Beat M. Frey, Martin Sill, Sven Nahnsen, Marie Gauder, Konstantina Kapolou, Manuela Silginer, Tobias Weiss, Hans-Georg Wirsching, Patrick Roth, Michael Grotzer, Niklaus Krayenbühl, Oliver Bozinov, Luca Regli, Hans-Georg Rammensee, Elisabeth J. Rushing, Felix Sahm, Juliane S. Walz, Michael Weller, Marian C. Neidert

“…Despite recent advances in integrated genomics, the prognosis in children with high-risk medulloblastoma remains devastating, and new tumor-specific therapeutic approaches are needed. Here, we present an atlas of naturally presented T cell antigens in medulloblastoma. …”
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Leveraging genomic insights from the neglected malaria parasites P. malariae and P. ovale using selective whole genome amplification (SWGA) approach by Fathia Ben-Rached, Amit Kumar Subudhi, Chang Li, Mariah Alawi, Rohit Satyam, Sui Xu, Guoding Zhu, Raeece Naeem, Sara Mfarrej, Di Liu, Zenaida Stead, Caroline Askonas, Yaobao Liu, Jun Cao, Arnab Pain

“…Conclusions SWGA approach implemented here provides a powerful tool for enhancing genomic analysis of these neglected parasites, revealing population diversity, drug resistance markers, and hypervariable regions. …”
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Effectiveness of hCMV, mEF1a and mAct promoters on driving of foreign gene expression in transgenic zebrafish by . Alimuddin, G. Yoshizaki, O. Carman, T. Takeuchi

“…The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis.  Here, as the first step of study, we conducted experiment to select an appropriate construct that allows higher expression levels of masu salmon (Oncorhynchus masou) D6-desaturase gene in zebrafish (Danio rerio) in order to increase its activity for synthesizing EPA/DHA.  …”
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Identification and quantification of transposable element transcripts using Long-Read RNA-seq in Drosophila germline tissues by Rebollo, Rita, Gerenton, Pierre, Cumunel, Eric, Mary, Arnaud, Sabot, François, Burlet, Nelly, Gillet, Benjamin, Hughes, Sandrine, S. Oliveira, Daniel, Goubert, Clément, Fablet, Marie, Vieira, Cristina, Lacroix, Vincent

“…However, assigning short reads to their correct genomic location, and genomic feature is not trivial. Here we retrieved full-length cDNA  (TeloPrime, Lexogen) of Drosophila melanogaster gonads and sequenced them using Oxford Nanopore Technologies. …”
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PPARα-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinoma by Valentin Van den bossche, Julie Vignau, Engy Vigneron, Isabella Rizzi, Hannah Zaryouh, An Wouters, Jérôme Ambroise, Steven Van Laere, Simon Beyaert, Raphaël Helaers, Cédric van Marcke, Lionel Mignion, Elise Y. Lepicard, Bénédicte F. Jordan, Céline Guilbaud, Olivier Lowyck, Hajar Dahou, Antonella Mendola, Manon Desgres, Léo Aubert, Isabelle Gerin, Guido T. Bommer, Romain Boidot, Perrine Vermonden, Aurélien Warnant, Yvan Larondelle, Jean-Pascal Machiels, Olivier Feron, Sandra Schmitz, Cyril Corbet

“…Abstract Anti-epidermal growth factor receptor (EGFR) therapy (cetuximab) shows a limited clinical benefit for patients with locally advanced or recurrent/metastatic head and neck squamous cell carcinoma (HNSCC), due to the frequent occurrence of secondary resistance mechanisms. Here we report that cetuximab-resistant HNSCC cells display a peroxisome proliferator-activated receptor alpha (PPARα)-mediated lipid metabolism reprogramming, with increased fatty acid uptake and oxidation capacities, while glycolysis is not modified. …”
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A Dual Active Black Hole Candidate with Mass Ratio ~7:1 in a Disk Galaxy by Xiao Cao, Yan-Mei Chen, Yong Shi, Junfeng Wang, Zhijie Zhou, Min Bao, Qiusheng Gu, Alexei Moiseev, Luis C. Ho, Lan Wang, Guangquan Zeng

“…Moreover, good estimates of the masses of both BHs are not yet available to test this idea. Here we report the evidence of a dual AGN candidate with mass ratio ~7:1 located in an undisturbed disk galaxy. …”
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Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis by José Alberto Choreño-Parra, Luis Armando Jiménez-Álvarez, Ellis Daniela Maldonado-Díaz, Graciela Cárdenas, Luis Alejandro Fernández-Lopez, José Luis Soto-Hernandez, Marcela Muñoz-Torrico, Gustavo Ramírez-Martínez, Alfredo Cruz-Lagunas, Armando Vega-López, María Lilia Domínguez-López, Carlos Sánchez-Garibay, Parménides Guadarrama-Ortíz, Silvia Giono, Luis Antonio Jiménez-Zamudio, Shabaana A. Khader, Ethel A. García-Latorre, Citlaltepetl Salinas-Lara, Joaquín Zúñiga

“…However, their role in TBM is unknown. Here, we performed a cross-sectional analysis of circulating NK cellCID="C008" value="s" phenotype in a prospective cohort of TBM patients (n=10) using flow cytometry. …”
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Compensatory synaptotagmin-11 expression conceals parkinson’s disease-like phenotypes in parkin knockout mice by Nan Dong, Zhenli Xie, Anqi Wei, Yuxin Yang, Yongning Deng, Xu Cheng, Bianbian Wang, Yang Chen, Yuhao Gu, Jingyu Yao, Yuhao Qin, Chaowen Zheng, Xi Zhang, Yuqing Zhang, Xinjiang Kang, Guoqing Chen, Qiumin Qu, Changhe Wang, Huadong Xu

“…However, the mechanism underlying this discrepancy remains elusive. Here, we provide compelling evidence that the compensatory expression of synaptotagmin-11 (Syt11) plays a key role in concealing PD-associated phenotypes in parkin knockout (KO) mouse models. …”
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Duodenal Organoids From Metabolic Dysfunction-Associated Steatohepatitis Patients Exhibit Absorptive and Barrier Alterations by Alia Hadefi, Morgane Leprovots, Gilles Dinsart, Maryam Marefati, Marjorie Vermeersch, Daniel Monteyne, David Pérez-Morga, Anne Lefort, Frédérick Libert, Laurine Verset, Claire Liefferinckx, Christophe Moreno, Jacques Devière, Eric Trépo, Marie-Isabelle Garcia

“…Recent research has emphasized the importance of the gut-liver axis in its pathogenesis, an aspect less explored in human studies. Here, we investigated whether the duodenal epithelium of MASH patients could exhibit intrinsic dysfunctions. …”
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LRRK2 Mutations and Asian Disease-Associated Variants in the First Parkinson’s Disease Cohort from Kazakhstan by Rauan Kaiyrzhanov, Akbota Aitkulova, Chingiz Shashkin, Nazira Zharkinbekova, Mie Rizig, Elena Zholdybayeva, Zharkyn Jarmukhanov, Vadim Akhmetzhanov, Gulnaz Kaishibayeva, Talgat Khaibullin, Altynay Karimova, Serik Akshulakov, Askhat Bralov, Nurlan Kissamedenov, Zhanar Seidinova, Anjela Taskinbayeva, Aliya Muratbaikyzy, Henry Houlden

“…Peculiar geographic distribution of these mutations has triggered an interest in genotyping PD cohorts of different ethnic backgrounds for LRRK. Objective. Here, we report on the results of LRRK2 screening in the first Central Asian PD cohort. …”
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Understanding the Broad-line Region of Active Galactic Nuclei with Photoionization. I. The Moderate-accretion Regime by Qiaoya Wu, Yue Shen, Hengxiao Guo, Scott F. Anderson, W. N. Brandt, Catherine J. Grier, Patrick B. Hall, Luis C. Ho, Yasaman Homayouni, Keith Horne, Jennifer I-Hsiu Li, Donald P. Schneider

“…., mass and Eddington ratio) reveal a substantial intrinsic dispersion around the average R – L relation, suggesting that variations in the broadband spectrum, driven by accretion parameters and other factors such as the cloud distribution and inclination, significantly influence the measured R – L relation. Here we perform a detailed photoionization investigation of expected broad-line properties as functions of accretion parameters using AGN continuum models from qsosed . …”
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Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice by Christian Thome, Jan Maximilian Janssen, Seda Karabulut, Claudio Acuna, Elisa D'Este, Stella J Soyka, Konrad Baum, Michael Bock, Nadja Lehmann, Johannes Roos, Nikolas A Stevens, Masashi Hasegawa, Dan A Ganea, Chloé M Benoit, Jan Gründemann, Lia Y Min, Kalynn M Bird, Christian Schultz, Vann Bennett, Paul M Jenkins, Maren Engelhardt

“…Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the native Ank3 gene. …”
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Activity-based chemical proteomics reveals caffeic acid ameliorates pentylenetetrazol-induced seizures by covalently targeting aconitate decarboxylase 1 by Guanjun Li, Ling Huang, Di Gu, Peili Wang, Letai Yi, Wenhua Kuang, Ying Zhang, Junzhe Zhang, Dandan Liu, Qiaoli Shi, Huan Tang, Jichao Sun, Guohua Zeng, Xin Peng, Jigang Wang

“…The anti-inflammatory effects of caffeic acid in LPS-activated BV2 cells was proved by knocking down the target protein. Results Here, we found that caffeic acid exhibits antiepileptic effects in pentylenetetrazol-induced epilepsy mice and exerts anti-neuroinflammation effect in vivo and in vitro. …”
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Extracellular viral microRNAs as biomarkers of virus infection in human cells by Cheryl Chan, Joanne Xin Yi Loh, Wei-Xiang Sin, Denise Bei Lin Teo, Nicholas Kwan Zen Tan, Chandramouli Nagarajan, Yunxin Chen, Francesca Lorraine Wei Inng Lim, Michael E. Birnbaum, Rohan B.H. Williams, Stacy L. Springs

“…Nucleic acid amplification tests (NAATs) have enabled fast and sensitive detection of virus infections but are unable to discriminate between live and dead/inert viral fragments or between latent and reactivated virus infections. Here, we show that extracellular viral microRNAs (viral exmiRs) are cell-free candidate biomarkers of live, latent, and reactivated virus infections, achieving fast (under 1 day) and sensitive (30 attomolar [aM]) detection by quantitative real-time reverse transcription PCR (real-time RT-qPCR). …”
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Clinical implementation of next-generation sequencing testing and genomically-matched therapy: a real-world data in a tertiary hospital by Jin Won Kim, Hee Young Na, Sejoon Lee, Ji-Won Kim, Koung Jin Suh, Se Hyun Kim, Yu Jung Kim, Keun-Wook Lee, Jong Seok Lee, Jaihwan Kim, Jin-Hyeok Hwang, Kihwan Hwang, Chae-Yong Kim, Yong Beom Kim, Soomin Ahn, Kyu Sang Lee, Hyojin Kim, Hye Seung Lee, So Yeon Park, Gheeyoung Choe, Jee Hyun Kim, Jin-Haeng Chung

“…Abstract Next-generation sequencing (NGS) cancer profiling has gained traction in routine clinical practice in South Korea. Here, we evaluated the use of NGS testing and genomically-matched therapies for patients with advanced solid tumors in a real-world clinical practice. …”
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