CD4 T cell depletion increases memory differentiation of endogenous and CAR T cells and enhances the efficacy of Super2 and IL-33-armored CAR T cells against solid tumors by Chrystal Paulos, Mary Jo Turk, Megen C Wittling, Yina H Huang, Asmaa O Mohamed, David Tyler Boone, Shannon L Ferry, Melanie C Peck, Alicia M Santos, Haille E Soderholm

“…Multiple strategies improve persistence by increasing stem-like properties or sustaining CAR T cell activity with combination therapies. Here, we describe the intrinsic ability of CAR T cells to differentiate into memory T cells, the effect of cytokine armoring, and neoadjuvant CD4 depletion therapy on CAR and tumor-specific endogenous memory T cells.Methods TRP1-specific or NKG2D CAR T cells alone or with Super2+IL-33 (S233) armoring and/or CD4 depletion were evaluated in immunocompetent B16F10 melanoma or MC38 colon cell carcinoma models without preconditioning. …”
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Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries by Javier Abello, Ying Yin, Yonghui Zhao, Josh Maurer, Jihui Lee, Cherokee Bodell, Jahmiera Richee, Abigail J. Clevenger, Zarek Burton, Megan E. Goeckel, Michelle Lin, Stephanie Grainger, Carmen M. Halabi, Shreya A. Raghavan, Rajan Sah, Amber N. Stratman

“…Vascular stabilization is a mechanosensitive process, in part driven by blood flow. Here, we demonstrate the involvement of the mechanosensitive ion channel, Piezo1, in promoting arterial accumulation of vascular smooth muscle cells (vSMCs) during zebrafish development. …”
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Ten practical guidelines for microclimate research in terrestrial ecosystems by Pieter De Frenne, Rémy Beugnon, David Klinges, Jonathan Lenoir, Pekka Niittynen, Sylvain Pincebourde, Rebecca A. Senior, Juha Aalto, Kryštof Chytrý, Phillipa K. Gillingham, Caroline Greiser, Eva Gril, Stef Haesen, Michael Kearney, Martin Kopecký, Peter C. le Roux, Miska Luoto, Ilya Maclean, Matěj Man, Rachel Penczykowski, Liesbeth van denBrink, Stijn Van de Vondel, Karen De Pauw, Jonas J. Lembrechts, Julia Kemppinen, Koenraad Van Meerbeek

“…Overviews of the protocols and measurement methods related to microclimate are needed, especially for those starting in the field and to achieve more generality and standardisation in microclimate studies. Here, we present 10 practical guidelines for ground‐based research of terrestrial microclimates, covering methods and best practices from initial conceptualisation of the study to data analyses. …”
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Comparison of PD-L1 tumor cell expression with 22C3, 28-8, and SP142 IHC assays across multiple tumor types by Jeffrey S Ross, Richard S P Huang, Jake G Maule, Lani K Clinton, Ryon P Graf, Jinpeng Xiao, Geoffrey R Oxnard

“…Background Multiple PD-L1 immunohistochemistry (IHC) assays, including DAKO 22C3, DAKO 28-8, and Ventana SP142 PD-L1 IHC assays, have been approved by the Food and Drug Administration as a companion diagnostic (CDx) for various antiprogrammed death-1 and antiprogrammed death ligand 1 (PD-L1) based cancer immunotherapies. Here we present 22C3, 28-8, and SP142 analysis of 418 tumor specimens encountered in routine clinical practice.Methods All specimens were tested with 22C3, 28-8, and SP142 assays following the manufacturer’s established staining protocols.Results The same PD-L1 status (defined as tumor cell expression (TC) scores with all three assays ≥1% or all <1%) was observed in 60.0% (251/418) tumor specimens (45.9% (192/418) were triple negative and 14.1% (59/418) were triple positive). …”
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High-performance laser speckle contrast image vascular segmentation without delicate pseudo-label reliance by Shenglan Yao, Huiling Wu, Suzhong Fu, Shuting Ling, Kun Wang, Hongqin Yang, Yaqin He, Xiaolan Ma, Xiaofeng Ye, Xiaofei Wen, Qingliang Zhao

“…In addition, supervised learning methods heavily rely on high-quality labels for accurate segmentation results, which often necessitate extensive labeling efforts. Here, we propose a novel approach LSWDP for high-performance real-time vessel segmentation that utilizes low-quality pseudo-labels for nonmatched training without relying on a substantial number of intricate labels and image pairing. …”
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Impact of a cardio‐oncology unit on prevention of cardiovascular events in cancer patients by Alessandra Cuomo, Valentina Mercurio, Gilda Varricchi, Maria Rosaria Galdiero, Francesca Wanda Rossi, Antonio Carannante, Grazia Arpino, Luigi Formisano, Roberto Bianco, Chiara Carlomagno, Carmine De Angelis, Mario Giuliano, Elide Matano, Marco Picardi, Domenico Salvatore, Ferdinando De Vita, Erika Martinelli, Carminia Maria Della Corte, Floriana Morgillo, Michele Orditura, Stefania Napolitano, Teresa Troiani, Carlo G. Tocchetti

“…Abstract Aims As the world population grows older, the co‐existence of cancer and cardiovascular comorbidities becomes more common, complicating management of these patients. Here, we describe the impact of a large Cardio‐Oncology unit in Southern Italy, characterizing different types of patients and discussing challenges in therapeutic management of cardiovascular complications. …”
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Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer by Jeremy L. Pinyon, Georg vonJonquieres, Stephen L. Mow, Amr Al Abed, Keng‐Yin Lai, Mathumathi Manoharan, Edward N. Crawford, Stanley H. Xue, Sarah Smith‐Moore, Lisa J. Caproni, Sarah Milsom, Matthias Klugmann, Nigel H. Lovell, Gary D. Housley

“…In the emerging field of DNA and particularly RNA‐based gene therapies, vector‐free delivery platforms are identified as a key unmet need. Here, this work addresses these challenges through gene electrotransfer (GET) of “naked” polyanionic DNA/mRNA using a single needle form‐factor which supports “electro‐lens” based compression of the local electric field, and local control of tissue conductivity, enabling single capacitive discharge minimal charge gene delivery. …”
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Generation of live mice from haploid ESCs with germline-DMR deletions or switch by Yongjian Ma, Meng Yan, Zhenfei Xie, Hongling Zhang, Zhoujie Li, Yuanyuan Li, Suming Yang, Meiling Zhang, Wen Li, Jinsong Li

“…However, what imprinting network is essential for development is still unclear. Here, we establish a stepwise system to reconstruct a development-related imprinting network, in which diploid embryonic stem cells (ESCs) are derived by fusing between parthenogenetic (PG)- and androgenetic (AG)-haploid embryonic stem cells (haESCs) with different DMR deletions (termed Ha-Ha-fusion system), followed by tetraploid complementation to produce all-haESC fetuses. …”
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Tax on Vacant Houses and Housing Price Bubble in Shiraz by Sara Parang, zahra dehghan shabani, Ebrahim Hadian, Ali Asgary

“…In fact, traders add tax to the price of the property, and increasing tax rates, in return worsens the bubble. So, here selecting the optimal tax rate becomes critically important. …”
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CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp by Jie Zhang, Liqiang Zhang, Xiaoman Wu, Minhui Tao, Yang Chen, Mingxian Chang

“…Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. …”
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[18F]FB(ePEG12)12-exendin-4 noninvasive imaging of insulinoma negative for insulin immunostaining on specimen from endoscopic ultrasonography-guided fine needle aspiration: a case... by Daisuke Otani, Takaaki Murakami, Saeko Murakami, Ikuko Hanaoka, Hiroyuki Fujimoto, Yoichi Shimizu, Kanae Kawai Miyake, Kentaro Sakaki, Yohei Ueda, Daisuke Tanaka, Tsuyoshi Ohno, Hironori Shimizu, Naoki Uyama, Norishige Iizuka, Daisuke Yabe, Yuji Nakamoto, Nobuya Inagaki

“…We recently developed the novel fluorine-18-labeled exendin-4-based probe conjugated with polyethylene glycol, [18F]FB(ePEG12)12-exendin-4 (18F-exendin-4) for positron emission tomography (PET) imaging and reported its clinical benefit in a case of insulinoma in the pancreatic tail. We report here a case of insulinoma in the pancreatic head in which an EUS-FNA specimen was negative on insulin immunostaining while precise preoperative localization and conclusive evidence for curative enucleation was provided by 18F-exendin-4 PET/CT (Japan Registry of Clinical Trials; jRCTs051200156).…”
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Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal by Jianrong He, Yuda Cheng, Yan Ruan, Jiali Wang, Yanping Tian, Jiaqi Wang, Fengsheng Wang, Chen Zhang, Yixiao Xu, Lianlian Liu, Meng Yu, Jiangjun Wang, Binyu Zhao, Yue Zhang, Yi Yang, Gaoke Liu, Wei Wu, Ping He, Jiaxiang Xiong, He Huang, Junlei Zhang, Rui Jian

“…However, the role and the molecular mechanism of Dax1 in the maintenance of different pluripotency states are poorly understood. Here, we constructed a stable Dax1 knockout (KO) cell line using the CRISPR/Cas9 system to analyze the precise function of Dax1. …”
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PHARAOH: A collaborative crowdsourcing platform for phenotyping and regional analysis of histology by Kevin Faust, Min Li Chen, Parsa Babaei Zadeh, Dimitrios G. Oreopoulos, Alberto J. Leon, Ameesha Paliwal, Evelyn Rose Kamski-Hennekam, Marly Mikhail, Xianpi Duan, Xianzhao Duan, Mugeng Liu, Narges Ahangari, Raul Cotau, Vincent Francis Castillo, Nikfar Nikzad, Richard J. Sugden, Patrick Murphy, Safiyh S. Aljohani, Philippe Echelard, Susan J. Done, Kiran Jakate, Zaid Saeed Kamil, Yazeed Alwelaie, Mohammed J. Alyousef, Noor Said Alsafwani, Assem Saleh Alrumeh, Rola M. Saleeb, Maxime Richer, Lidiane Vieira Marins, George M. Yousef, Phedias Diamandis

“…However, its context-specific nature and continued reliance on large expert-annotated training datasets hinders the development of a critical mass of applications to garner widespread adoption in clinical/research workflows. Here, we present an online collaborative platform that streamlines tissue image annotation to promote the development and sharing of custom computer vision models for PHenotyping And Regional Analysis Of Histology (PHARAOH; https://www.pathologyreports.ai/ ). …”
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An interactive course program on nutrition for medical students: interdisciplinary development and mixed-methods evaluation by Gonza B. Ngoumou, Daniela A. Koppold, Laetitia Wenzel, Anne Schirmaier, Carolin Breinlinger, Lisa M. Pörtner, Stefan Jordan, Julia K. Schiele, Etienne Hanslian, Annika Koppold, Beate Stock-Schröer, Dimitra M. Varvarezou, Michael Jeitler, Miriam Ortiz, Andreas Michalsen, Wiebke Stritter, Georg Seifert, Christian S. Kessler

“…The relevance of nutrition for individual and planetary health is insufficiently addressed in German medical schools. Here, we present a two-week course for medical students in the 8th semester at Charité - Universitätsmedizin Berlin on nutrition and fasting in the context of human and planetary health. …”
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Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function by Annat Raiter, Yael Barhum, Julia Lipovetsky, Chen Menachem, Sharona Elgavish, Shmuel Ruppo, Yehudit Birger, Shai Izraeli, Orna Steinberg-Shemer, Rinat Yerushalmi

“…We recently demonstrated that Galectin-3 (Gal-3) binding protein/Gal-3 complex secreted by TNBC cells induces immunosuppression, through inhibiting CD45 signaling in T cells. Here, we further investigated the interaction between secreted Gal-3 and T cells in TNBC.Using CRISPR/Cas9 gene editing of the TNBC MDA-MB-231 cell-line, we obtained Gal-3 negative(neg) clones. …”
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A compilation of surface inherent optical properties and phytoplankton pigment concentrations from the Atlantic Meridional Transect by T. M. Jordan, G. Dall'Olmo, G. Tilstone, R. J. W. Brewin, F. Nencioli, R. Airs, C. S. Thomas, L. Schlüter

“…<p>In situ measurements of particulate inherent optical properties (IOPs) – absorption (<span class="inline-formula"><i>a</i>_p(<i>λ</i>)</span>), scattering (<span class="inline-formula"><i>b</i>_p(<i>λ</i>)</span>), and beam attenuation (<span class="inline-formula"><i>c</i>_p(<i>λ</i>)</span>) – are crucial for the development of optical algorithms that retrieve biogeochemical quantities such as chlorophyll <span class="inline-formula"><i>a</i></span>, particulate organic carbon (POC), and total suspended matter (TSM). Here we present a compilation of particulate absorption–attenuation spectrophotometric data measured underway on nine Atlantic Meridional Transect (AMT) cruises between 50° N and 50° S from 2009–2019. …”
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The AMD-associated genetic polymorphism CFH Y402H confers vulnerability to Hydroquinone-induced stress in iPSC-RPE cells by Angela Armento, Inga Sonntag, Ana-Cristina Almansa-Garcia, Merve Sen, Sylvia Bolz, Blanca Arango-Gonzalez, Ellen Kilger, Ruchi Sharma, Kapil Bharti, Rosario Fernandez-Godino, Berta de la Cerda, Simon J. Clark, Simon J. Clark, Simon J. Clark, Marius Ueffing

“…In our study, we examined the cell characteristics of the retinal pigment epithelium (RPE) cells, which play a major role in retinal homeostasis and stability and which are synonymously linked to AMD.MethodsHere, we employ RPE cells derived from induced pluripotent stem cells (iPSC) generated from donors, carrying either homozygous 402Y (low risk) or 402H (high risk) variants of the CFH gene. …”
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The transcriptional analysis of pepper shed light on a proviral role of light-harvesting chlorophyll a/b binding protein 13 during infection of pepper mild mottle virus by Weihong Lin, Shugen Zhang, Hao Zhang, Xiaomei Deng, Tong Jiang, Xifeng Chen, Laihua Dong, Qin Yan, Lianyi Zang, Yongping Xing, Zhenquan Wang, Qin Zhang, Kaitong Du, Huolin Shen, Junmin Zhang, Tao Zhou

“…Despite its impact, the pathogenicity mechanisms of PMMoV and the pepper plant’s response to infection remain poorly understood. Here, we compared the transcriptomic changes in a susceptible pepper inbred line 21C241 with a resistant inbred line 21C385 seedlings, following systemic PMMoV infection using RNA sequencing. …”
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IFN-γ reprograms cardiac microvascular endothelial cells to mediate doxorubicin transport and influences the sensitivity of mice to doxorubicin-induced cardiotoxicity by Haoyu Ji, Wenya Ma, Xu Liu, Hongyang Chen, Yining Liu, Zhongyu Ren, Daohong Yin, Ao Cai, Zizhen Zhang, Xin Wang, Wei Huang, Leping Shi, Yanan Tian, Yang Yu, Xiuxiu Wang, Yang Li, Yu Liu, Benzhi Cai

“…The sensitivity to DIC varies among patients, but the precise mechanisms remain elusive. Here we constructed a mouse model of DIC using DOX to investigate potential mechanisms contributing to the differential susceptibility to DIC. …”
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