Comparison of lateral tail vein and retro-orbital venous sinus as routes of inoculation to study Group B streptococcal systemic infection by Jéssica da Conceição Mendonça, Stephen Lumsdaine, Lindsey R. Burcham

“…In this study, one of the leading bacterial meningeal pathogens, Streptococcus agalactiae, or Group B Streptococcus (GBS), was used to compare experimental outcomes of two commonly used routes of hematogenous infection, lateral tail vein injection and retro-orbital venous sinus injection. Here we demonstrate that both routes of infection result in systemic disease and the onset of clinical symptoms of infection. …”
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Insights into mapping tropical primary wet forests in the Amazon Basin from satellite-based time series metrics of canopy stability by Brendan Mackey, Sonia Hugh, Tatiana Shestakova, Brendan M. Rogers, Ludmila Rattis

“…High resolution modelled data on forest structure can be usefully complemented in tropical wet forests by the kinds of time series analyses presented here, which can assist in tracking changes in forest condition and responses to disturbances.…”
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Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections. by Nils Pilotte, Victor Omballa, Monica Voss, Leah Padgett, Malathi Manuel, Jeanne L Goodman, Tim Littlewood, Zayina Zondervenni Manoharan, Lisette van Lieshout, Jaco J Verweij, Manigandan Sekar, Ajith Kumar Muthukumar, Gretchen Walch, Abriana Warren, Mariyam Sheikh, Andrew Gonzalez, Sean R Galagan, Sitara Swarna Rao Ajjampur, Moudachirou Ibikounlé, Steven A Williams, Doug Rains, Ushashi Dadwal, Judd L Walson

“…<h4>Conclusions/significance</h4>Resulting from the rigorous developmental approach taken, the platform we describe here provides the needed confidence in testing outcomes when utilized in conjunction with large-scale efforts such as the DeWorm3 trial. …”
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Species specificity and specificity diversity (SSD) framework: a novel method for detecting the unique and enriched species associated with disease by leveraging the microbiome het... by Zhanshan Sam Ma

“…The second element is a new concept to introduce here, the (species) specificity diversity (SD), which is inspired by traditional species (abundance) diversity in community ecology and measures the diversity of specificity (a proxy for metacommunity heterogeneity, essentially) with Renyi’s entropy. …”
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Antiepileptic activity and potential mechanism of full-spectrum hemp extract by Zengliang Jin, Zhuo Huang

“…The effect and underlying mechanism of full-spectrum hemp extract (HE) remains unclear. Here this study investigated the anti-seizure effect of HE on seizure models. …”
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Identification and semi-quantification of protein allergens in complex mixtures using proteomic and AllerCatPro 2.0 bioinformatic analyses: a proof-of-concept investigation by Nora L. Krutz, Ian Kimber, Jason Winget, Minh N. Nguyen, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Catherine Mahony, G. Frank Gerberick

“…However, no method has yet been accepted or validated for assessment of potential allergenic hazards in such materials. In the studies here, a dual proteomic-bioinformatic approach is proposed to evaluate holistically allergenic hazards in complex mixtures of plants, insects, or animal proteins. …”
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Perinatal Development of the Motor Systems Involved in Postural Control by Laurent Vinay, Faïza Ben-Mabrouk, Frédéric Brocard, François Clarac, Céline Jean-Xavier, Edouard Pearlstein, Jean-François Pflieger

“…Motor behaviors of some species, such as the rat and the human baby, are quite immature at birth. Here we review recent data on some of the mechanisms underlying the postnatal maturation of posture in the rat, in particular the development of pathways descending from the brain stem and projecting onto the lumbar enlargement of the spinal cord. …”
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About Takhcheia in the letter of commendation to Yakov and Grigory Stroganov dated may 30, 1574. by Korchagin P.A., Samigulov G.Kh

“…The Stroganovs were interested in joining the Takhcheya region to their possessions, since trade routes, that passed here, connected the Urals with Central Asia, in particular, Bukhara and Samarkand. …”
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RUBY® – a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties by Barnabas Nyesiga, Mattias Levin, Anna Säll, Anna Rosén, Kim Jansson, Sara Fritzell, Karin Hägerbrand, Dietmar Weilguny, Laura von Schantz

“…Despite the large number of existing bispecific antibody (bsAb) formats, the generation of novel bsAbs is still associated with development and bioprocessing challenges. Here, we present RUBY, a novel bispecific antibody format that allows rapid generation of bsAbs that fulfill key development criteria. …”
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Mapping C. difficile TcdB interactions with host cell-surface and intracellular factors using proximity-dependent biotinylation labeling by Jennifer S. Ward, Karl J. Schreiber, John Tam, Ji-Young Youn, Roman A. Melnyk

“…., BioID) is a powerful method to identify nearby host factors in living cells, offering the potential to identify host targets of microbial toxins. Here, we used BioID to interrogate proximal interactors of the multi-domain Clostridioides difficile TcdB toxin. …”
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Chemical Changes Under Heat Stress and Identification of Dendrillolactone, a New Diterpene Derivative with a Rare Rearranged Spongiane Skeleton from the Antarctic Marine Sponge <i>... by Andrea Prófumo, Conxita Avila, Adele Cutignano

“…Here, we also report our studies on the changes in the chemical arsenal of this sponge by slow temperature increase in aquaria experiments. …”
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Spatio‐Temporal Patterns of Hybridization in an Alloploid Salamander (Ambystomatidae: Ambystoma) and Conservation Implications of Introgression in a Unisexual Vertebrate by Alessa V. Laserna, Christopher A. Phillips, Andrew R. Kuhns, Mark A. Davis, Joel B. Corush, Ken N. Paige

“…Understanding the stability of these lineages over time can inform our understanding of the advantages of an asexual mode of life. Here we investigate a gynogenetic allopolyploid triploid, the Silvery Salamander (LJJ—historically referred to as Ambystoma platineum), an asexual lineage closely related to A. barbouri. …”
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A Deep Learning-Based Approach for Two-Phase Flow Pattern Classification Using Void Fraction Time Series Analysis by Jefferson Dos Santos Ambrosio, Marco Jose da Silva, Andre Eugenio Lazzaretti

“…The results demonstrate that the approach chosen here presents superior classification metrics in all cases evaluated, particularly in cross-dataset experiments. …”
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eIF3 engages with 3’-UTR termini of highly translated mRNAs by Santi Mestre-Fos, Lucas Ferguson, Marena I Trinidad, Nicholas T Ingolia, Jamie HD Cate

“…However, the molecular mechanisms underlying this translational burst and the involvement of initiation factors remains largely unknown. Here, we investigate the role of eukaryotic initiation factor 3 (eIF3) in early differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (NPCs). …”
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Persistence and emergence of X4 virus in HIV infection by Ariel D. Weinberger, Alan S. Perelson

“…Thus, highly active antiretroviral therapy (HAART), which increases CD4+ T cell counts and decreases cellular activation levels, inhibits X4 viral growth. However, we show here that even in the simplest dual-strain framework, competition between R5 and X4 viruses often results in accelerated X4 emergence in response to CCR5 inhibition, further highlighting the potential danger of anti-CCR5 monotherapy in multi-strain HIV infection.…”
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Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening by Channabasavaiah B. Gurumurthy, Poonam S. Joshi, Scott G. Kurz, Masato Ohtsuka, Rolen M. Quadros, Donald W. Harms, K. C. Kent Lloyd

“…The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens. …”
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In vivo interspecies dissemination of IncM2-type blaNDM-1 carrying plasmid by Mor N. Lurie-Weinberger, Darya Bychenko-Banyas, Meirav Mor, Sivan Laviad-Shitrit, Ella Kaplan, Nadya Rakovitsky, Alona Keren-Paz, Chaim Ben-Zvi, Yehuda Carmeli

“…Seven New Delhi metallo-beta-lactamase(NDM) positive and one NDM-negative bacteria were sent for WGS followed by bioinformatics analysis. Here, we describe three separate cases of patients simultaneously colonized by two NDM producing-species (Escherichia coli and Klebsiella pneumoniae), in which we documented interspecies dissemination of the blaNDM-1 by an 87,450 bp IncM2 type multi-drug resistance plasmid (here named pNCICAN_NDM-01_21-il). …”
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Molecular mechanisms and comparative transcriptomics of diapause in two corn rootworm species (Diabrotica spp.) by Melise C. Lecheta, Chad Nielson, B. Wade French, Emily A.W. Nadeau, Nicholas M. Teets

“…Diapause is a programmed developmental arrest that can occur at any developmental stage depending on species, but the mechanisms that underscore embryonic diapause are poorly understood. Here, we identified molecular mechanisms underscoring distinct phases of diapause in the Diabrotica spp. complex. …”
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Optimizing fully-efficient two-stage models for genomic selection using open-source software by Javier Fernández-González, Julio Isidro y Sánchez

“…However, unweighted (UNW) two-stage models assume independent errors among adjusted means, neglecting correlations among estimation errors. Here, we show that fully-efficient two-stage models perform similarly to UNW models for randomized complete block designs but substantially better for augmented designs. …”
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A reproducible representation of healthy tibiofemoral kinematics during stair descent using REFRAME – part I: REFRAME foundations and validation by Ariana Ortigas-Vásquez, William R. Taylor, Barbara Postolka, Pascal Schütz, Allan Maas, Matthias Woiczinski, Thomas M. Grupp, Adrian Sauer

“…Consequently, for consistent interpretation of joint motion (especially direct comparison) to be possible, differences in local frame position and orientation must first be addressed. Here, building on previous work that introduced a frame orientation optimisation method and demonstrated its potential to induce convergence towards a consistent kinematic signal, we present the REference FRame Alignment MEthod (REFRAME) that addresses both rotational and translational kinematics, is validated here for a healthy tibiofemoral joint, and allows flexible selection of optimisation criteria to fittingly address specific research questions. …”
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