Glycoconjugates and Related Molecules in Human Vascular Endothelial Cells by Norihiko Sasaki, Masashi Toyoda

“…In this paper, we consider the expression and functional roles of endogenous glycoconjugates and related molecules (galectins and glycan-degrading enzymes) in human ECs.…”
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Biochemical characterization of the Escherichia coli surfaceome: a focus on type I fimbriae and flagella by Devon W. Kavanaugh, Adeline Sivignon, Yannick Rossez, Zina Chouit, Christophe Chambon, Christophe Chambon, Louane Béal, Mathilde Bonnet, Michel Hébraud, Michel Hébraud, Yann Guérardel, Hang Thi Thu Nguyen, Nicolas Barnich

“…Using biochemical and glycobiological techniques, including biotin-hydrazide labeling of glycans and chemical and glycosidase treatments, we demonstrate (i) the presence of a well-defined and chemically resistant FimA oligomer in several strains of pathogenic and non-pathogenic E. coli, (ii) the major subunit of type I fimbriae, FimA, in pathogenic and laboratory strains is recognized by concanavalin A, (iii) standard methods to remove N-glycans (PNGase F) or a broad-specificity mannosidase fail to remove the glycan structure, despite the treatments resulting in altered migration in SDS-PAGE, (iv) PNGase F treatment results in a novel 32 kDa band recognized by anti-FliC antiserum. …”
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Identification of potential druggable targets for endometriosis through Mendelian randomization analysis by Peng Chen, Xin Wei, Xiao-Ke Li, Yi-Hang Zhou, Yi-Hang Zhou, Qi-Fang Liu, Ling Ou-Yang

“…Additionally, mechanistic roles of FN1, glycan degradation pathway, LGALS3, CPE, and FUT5 in EM warrant further investigation.…”
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A self-assembled nanoparticle vaccine elicits effective neutralizing antibody response against EBV infection by Ping Li, Ping Li, Ziyi Jiang, Jingjing Shi, Haochuan Sha, Zihang Yu, Yan Zhao, Sanyang Han, Lan Ma, Lan Ma, Lan Ma

“…These epitope peptides detected a binding capability with host cells were then fused by flexibility linkers and expressed in Escherichia coli to reduce the unnecessary glycan modifications to simulate their free-glycan status. …”
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A 2-decade survival from a double hemolytic disease: A case of paroxysmal nocturnal hemoglobinuria with hemoglobinopathy by Aria Namiq Chaqmachi, Brwa Ali Hussein

“…Due to a mutation in the phosphatidylinositol glycan class A (PIGA) gene, hemolytic anemia, thrombosis, and bone marrow failure are the hallmarks of the uncommon hematologic illness known as paroxysmal nocturnal hemoglobinuria (PNH). …”
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Toward spatial glycomics and glycoproteomics: Innovations and applications by Patcharaporn Boottanun, Sayaka Fuseya, Atsushi Kuno

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Biosynthesis and Role of N-Linked Glycosylation in Cell Surface Structures of Archaea with a Focus on Flagella and S Layers by Ken F. Jarrell, Gareth M. Jones, Divya B. Nair

“…Structures of archaeal N-linked glycans have indicated a variety of linking sugars as well as unique sugar components. …”
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Conserved role of spike S2 domain N-glycosylation across betacoronaviruses by Qi Yang, Anju Kelkar, Balaji Manicassamy, Sriram Neelamegham

“…Abstract Besides acting as an immunological shield, the N-glycans of SARS-CoV-2 are also critical for viral life cycle. …”
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Discovery of Nanosota-EB1 and -EB2 as Novel Nanobody Inhibitors Against Ebola Virus Infection. by Fan Bu, Gang Ye, Kimberly Morsheimer, Alise Mendoza, Hailey Turner-Hubbard, Morgan Herbst, Benjamin Spiller, Brian E Wadzinski, Brett Eaton, Manu Anantpadma, Ge Yang, Bin Liu, Robert Davey, Fang Li

“…Cryo-EM and biochemical data revealed that Nanosota-EB1 binds to the glycan cap of GP1, preventing its protease cleavage, while Nanosota-EB2 binds to critical membrane-fusion elements in GP2, stabilizing it in the pre-fusion state. …”
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Quantitative glycoproteomics of human milk and association with atopic disease. by Matilda Holm, Mayank Saraswat, Sakari Joenväärä, Antti Seppo, R John Looney, Tiialotta Tohmola, Jutta Renkonen, Risto Renkonen, Kirsi M Järvinen

“…We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. We found that the abundances of 38 glycopeptides differed significantly between Rochester and OOM mothers and also identified four glycopeptides with significantly different abundances between all comparisons. …”
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Unraveling the impact of SARS-CoV-2 mutations on immunity: insights from innate immune recognition to antibody and T cell responses by Rafael Bayarri-Olmos, Rafael Bayarri-Olmos, Adrian Sutta, Adrian Sutta, Anne Rosbjerg, Anne Rosbjerg, Mie Mandal Mortensen, Charlotte Helgstrand, Per Franklin Nielsen, Laura Pérez-Alós, Beatriz González-García, Laust Bruun Johnsen, Finn Matthiesen, Thomas Egebjerg, Cecilie Bo Hansen, Alessandro Sette, Alessandro Sette, Alba Grifoni, Ricardo da Silva Antunes, Peter Garred, Peter Garred

“…Notably, we identified two glycan sites (N717 and N801) as potentially essential for the structural integrity of the spike protein. …”
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De novo design of protein minibinder agonists of TLR3 by Chloe S. Adams, Hyojin Kim, Abigail E. Burtner, Dong Sun Lee, Craig Dobbins, Cameron Criswell, Brian Coventry, Adri Tran-Pearson, Ho Min Kim, Neil P. King

“…Cryo-EM structures of two minibinders in complex with TLR3 reveal that they bind the target as designed, although one partially unfolds due to steric competition with a nearby N-linked glycan. Multivalent forms of both minibinders induce NF-κB signaling in TLR3-expressing cell lines, demonstrating that they may have therapeutically relevant biological activity. …”
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Mutant glycosidases for labeling sialoglycans with high specificity and affinity by Shuyu Liang, Qi Tang, Xunzi Guo, Zi’an Li, Yilan Guo, Jinghan Chang, Bo Cheng, Qitao Song, Jiayu Sun, Peng Dai, Xing Chen

“…However, affinity probes recognizing glycans with high specificity remain scarce. Here we report the development of glycan recombinant affinity binders (GRABs) based on mutant bacterial sialidases, which are enzymatically inactive but preserve stringent specificity for sialoglycan substrates. …”
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A limited number of antibody specificities mediate broad and potent serum neutralization in selected HIV-1 infected individuals. by Laura M Walker, Melissa D Simek, Frances Priddy, Johannes S Gach, Denise Wagner, Michael B Zwick, Sanjay K Phogat, Pascal Poignard, Dennis R Burton

“…In approximately half of the donors, key N-linked glycans were critical for expression of the epitopes recognized by the bNAb specificities in the sera.…”
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Distinct vaginal microbiome and metabolome profiles in women with preterm delivery following cervical cerclage by Jun Zhang, Li Li, Mengjun Zhang, Jiaoning Fang, Zhimin Xu, Yijing Zheng, Zhi Lin, Mian Pan

“…In addition, vaginal metabolomics-based liquid chromatography–Orbitrap–tandem mass spectrometry (LC-Orbitrap-MS/MS) found that the alteration in vaginal metabolites in pregnant women with PTB is involved in starch and sucrose metabolism; arginine and praline metabolism; galactose metabolism; purine metabolism; arginine metabolism; tryptophan metabolism and N-glycan biosynthesis; cysteine and methionine metabolism; taurine and hypotaurine metabolism; amino acid metabolism; propanoate metabolism; valine, leucine, and isoleucine biosynthesis; glycine, serine, and threonine metabolism; and steroid hormone biosynthesis. …”
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The Evolution of Immunosuppressive Therapy in Pig-to-Nonhuman Primate Organ Transplantation by S. A. Sanatkar, K. Kinoshita, A. Maenaka, H. Hara, D. K. C. Cooper

“…Major steps were the identification and deletion of expression of the three known glycan xenoantigens on pig vascular endothelial cells, the transgenic expression of human “protective” proteins, e.g., complement-regulatory, coagulation-regulatory, and anti-inflammatory proteins, and the administration of an immunosuppressive regimen based on blockade of the CD40/CD154 T cell co-stimulation pathway. …”
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Pseudomonas aeruginosa pili and flagella mediate distinct binding and signaling events at the apical and basolateral surface of airway epithelium. by Iwona Bucior, Julia F Pielage, Joanne N Engel

“…Using two different cell-free systems, we demonstrate that Tfp-coated beads show highest binding affinity to complex N-glycan chains coated onto plastic plates and preferentially aggregate with beads coated with N-glycans, but not with single sugars or HS. …”
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Meta-analysis of RNA-Seq datasets allows a better understanding of P. tricornutum cellular biology, a requirement to improve the production of Biologics by Isabelle Boulogne, Charlotte Toustou, Muriel Bardor

“…Therefore, the expression of genes involved in the N-glycan biosynthesis, protein export and secretion, protein quality control and proteasome, as well as those encoding proteases were analyzed and compared. …”
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How the Knowledge of Interactions between Meningococcus and the Human Immune System Has Been Used to Prepare Effective Neisseria meningitidis Vaccines by R. Gasparini, D. Panatto, N. L. Bragazzi, P. L. Lai, A. Bechini, M. Levi, P. Durando, D. Amicizia

“…Capsular polysaccharide and lipooligosaccharide glycan composition, in particular, play a major role in circumventing immune response. …”
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CD22 modulation alleviates amyloid β-induced neuroinflammation by Yu Dong Mai, Qingqing Zhang, Cheuk Lim Fung, Shui On Leung, Chi Ho Chong

“…Moreover, sCD22 activated microglia via sialic acid binding domain and 2,6 linked sialic acid glycan on sCD22. The pivotal therapeutic potential of targeting CD22 was demonstrated in Amyloid β (Aβ) induced-neuroinflammation in hCD22 transgenic mice. …”
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