Unveiling the endogenous CRISPR-Cas system in Pseudomonas aeruginosa PAO1. by Javier Alejandro Delgado-Nungaray, Luis Joel Figueroa-Yáñez, Eire Reynaga-Delgado, Ana Montserrat Corona-España, Orfil Gonzalez-Reynoso

“…Additionally, we demonstrated a co-evolutionary relationship between the orphan CRISPR system in P. aeruginosa PAO1 and the mobile genetic element and prophages identified. One self-targeting spacer was identified, often associated with bacterial evolution and autoimmunity, and no Acr proteins. …”
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A Case of Hypophosphatasia Started Enzyme Replacement Therapy Since Babyhood Stage by Tatsuya Akitomo, Noriko Niizato, Ami Kaneki, Masashi Ogawa, Taku Nishimura, Mariko Kametani, Momoko Usuda, Yuko Iwamoto, Chieko Mitsuhata, Ryota Nomura

“…She started enzyme replacement therapy 6 days after birth, and genetic analysis later confirmed the diagnosis of HPP. …”
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Energy and exergy analysis of a modified air handling unit assisted by phase change material, heat recovery unit, and solar energy by Saman Faramarzi, Seyed Mohammad Mehdi Asadzadeh, Mohammad Reza Mohammad Hassani, Seyyed Amirreza Abdollahi, Reza Zarehzadeh, Mostafa Mafi

“…Through detailed energy and exergy analyses, the study evaluates the AHU’s performance and optimises the thickness and position of the PCM layer using genetic algorithm (GA) optimisation. Simulation of the proposed system was conducted using ASPEN Hysys and MATLAB software. …”
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Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies by Qi Zhang, Min Li, Wenbo Hu, Xin Wang, Jinlian Hu

“…Beginning with a brief description of biological and mechanical properties of spidroin-based materials and the cellular regulatory mechanism, this review summarizes various types of spidroin-based biomaterials from genetically engineered spider silks and their prospects for specific biomedical applications (e.g., lung tissue engineering, vascularization, bone and cartilage regeneration, and peripheral nerve repair), and finally, we prospected the development direction and manufacturing technology of building more refined and customized spidroin-based protein scaffolds.…”
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Intestinal Type of Lung Adenocarcinoma in Younger Adults by Jelena Stojsic, Milica Kontic, Dragan Subotic, Marko Popovic, Dragana Tomasevic, Jelena Lukic

“…Colorectal adenocarcinoma claimed to be genetically similar to an ILADC. Patients. We describe 24- and 26-year-old patients of both genders who went under surgery because of a lung tumor mass detected on CT scan. …”
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Diversity and Potential Cross-Species Transmission of Rotavirus A in Wild Animals in Yunnan, China by Xiang Le, Yinzhu Tao, Binghui Wang, Yutong Hou, Yuting Ning, Junjie Hou, Ruimei Wang, Qian Li, Xueshan Xia

“…Next generation sequencing and phylogenetic analyses revealed a high degree of genetic diversity and reassortment, particularly for VP4 and VP7. …”
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Molecular analysis of zoonotic pathogens in free-ranging six-banded armadillos (Euphractus sexcinctus) from the Brazilian semiarid region by Ilanna Vanessa Pristo de Medeiros Oliveira, José Artur Brilhante Bezerra, Gabriela Hémylin Ferreira Moura, Ana Carolina Yamakawa, Mariana Guimarães Nilsson, Jéssica da Silva Ferreira, Amanda Haisi, Felipe Fornazari, Hélio Langoni, João Marcelo Azevedo de Paula Antunes

“…All tests presented negative results apart from whole blood PCR to detect T. cruzi, which was positive in one of the 20 animals tested and confirmed by genetic sequencing. It is important to highlight that this is the first study comprising a molecular investigation of different zoonotic pathogens in six-banded armadillos, and the findings reported here bring new and important knowledge regarding zoonotic diseases in this species.…”
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Electricity Usage Scheduling in Smart Building Environments Using Smart Devices by Eunji Lee, Hyokyung Bahn

“…An efficient heuristic based on genetic algorithms is performed on a smart device to cut down the huge searching space and find a reasonable schedule within a feasible time budget. …”
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A Link between Autophagy and the Pathophysiology of LRRK2 in Parkinson's Disease by Patricia Gómez-Suaga, Elena Fdez, Marian Blanca Ramírez, Sabine Hilfiker

“…Mutations in leucine-rich repeat kinase 2 (LRRK2) cause dominantly inherited PD, and variations increase risk, indicating that LRRK2 is an important player in both genetic and sporadic forms of the disease. G2019S, the most prominent pathogenic mutation, maps to the kinase domain and enhances enzymatic activity of LRRK2, which in turn seems to correlate with cytotoxicity. …”
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Fully automated extraction of high-quality total nucleic acids from FFPE specimens for comprehensive genomic profiling of solid tumors by Kristopher Amirault, Michael Collins, Luca Beker, Brandon Mills, Martina Werner, Jonathan Andreas, Daniel Hartman, Jordan Dargert, Vanessa Process, Sean Cederlund, Thuy Dao, Linnea Menin, Molly Ferrara, Andrew Briggs, Joshua Shreve, Daniel Metzger, Angela Stout, Erin Deblasi, Jie An, Taylor Jensen, Shengle Zhang, Vinesh Rana, Bryn Burlingame, Ulrich Thomann, Durga Prasad Dash, Sha Liao, Jeffrey M. Conroy, Eugenio Daviso

“…Formalin-fixed paraffin-embedded tissues are notoriously difficult samples in respect to reliably extracting high-quality nucleic acids and sufficient genetic material to meet sequencing input requirements. …”
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Hmox1 Deficiency Sensitizes Mice to Peroxynitrite Formation and Diabetic Glomerular Microvascular Injuries by Olivia Lenoir, François Gaillard, Hélène Lazareth, Blaise Robin, Pierre-Louis Tharaux

“…This was associated with a renal increase in the expression of the oxidative stress marker, nitrotyrosine. Conclusions. Genetic Hmox1 partial deficiency is sufficient to sensitize mice to the development of diabetic glomerular microvascular lesions. …”
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A sparrow search algorithm based hybrid meta-heuristic algorithm for population growth rate prediction by Milad Shahvaroughi Farahani, Hamed Farrokhi-Asl Farrokhi-Asl, Ghazal Ghasemi

“…A set of 18 economic indicators serves as input variables, and a Genetic Algorithm (GA) is used for feature selection. …”
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Promising Remission with Reduced Bevacizumab and Pembrolizumab Dosage in a Patient with AT-rich Interaction Domain 1A Mutated Ovarian Clear-cell Carcinoma Refractory to Chemotherap... by Cheng-Wei Huang, Ruo-Han Tseng

“…Immune checkpoint inhibitors may play a new role in the treatment of these malignancies. Genetic analyses revealed a significant proportion of ovarian clear-cell carcinoma carrying the AT-rich interaction domain 1A protein (ARID1A) mutation. …”
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The Role of Neuroglia in the Development and Progression of Schizophrenia by Neha S. Rawani, Allen W. Chan, Kathryn G. Todd, Glen B. Baker, Serdar M. Dursun

“…Schizophrenia is a complex heterogenous disorder thought to be caused by interactions between genetic and environmental factors. The theories developed to explain the etiology of schizophrenia have focused largely on the dysfunction of neurotransmitters such as dopamine, serotonin and glutamate with their receptors, although research in the past several decades has indicated strongly that other factors are also involved and that the role of neuroglial cells in psychotic disorders including schizophrenia should be given more attention. …”
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Isolation of MDCK cells with low expression of mdr1 gene and their use in membrane permeability screening by BOKULIĆ ANA, PADOVAN JASNA, STUPIN-POLANČEC DARIJA, MILIĆ ASTRID

“…Because MDCK wild type (WT) is genetically heterogeneous, an isolation procedure was investigated in this study to obtain the subclonal line with low P-glycoprotein expression. …”
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Bardet-Biedl Syndrome, Crohn Disease, Primary Sclerosing Cholangitis, and Autoantibody Positive Thyroiditis: A Case Report and A Review of a Cohort of BBS Patients by Ugur Halac, Denise Herzog

“…Bardet-Biedel syndrome (BBS) is a rare autosomal recessive, genetically heterogeneous ciliopathy. Although the disease has been described in a patient with psoriasis, individuals with BBS are not known to be at risk of developing autoimmune disorders. …”
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Trajectory Planning of Camellia Oleifera Pollen Picking Manipulators Based on an Improved Particle Swarm Optimization Algorithm by Li Jun, Zhao Qing, Li Lijun, Wu Zechao, Guo Xin, Fan Ziyan, Gong Hongbin

“…The comparison between traditional genetic algorithm (SGA) and traditional particle swarm optimization (SPSO) shows that the IPSO algorithm can be better applied to the time optimal trajectory planning of camellia oleifera pollen picking manipulators.…”
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Natural Killer Cell and Large Granular Lymphocyte Deficiency in the Gut of Children with Inflammatory Bowel Disease by F Hadziselimovic, LR Emmons, U Schaub

“…The lack of natural killer cells and LGL in the gut epithelium in children with IBD may be indicative of a possible genetic predisposition. The authors also present a new therapeutic strategy consisting of low dose interferon-alpha-2a that is efficacious in ameliorating ulcerative col iris and Crohn's disease and concomitantly increasing the number of natural killer cells and LGL in the gut.…”
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Collaborative Multidepot Petrol Station Replenishment Problem with Multicompartments and Time Window Assignment by Guangcan Xu, Maozeng Xu, Yong Wang, Yong Liu, Qiguang Lv

“…The hybrid heuristic algorithm composed of genetic algorithm and particle swarm optimization is used as a solution, and then the Shapley value method is applied to analyze the profit allocation of each petrol depot under different coalitions. …”
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An integrative approach to the delimitation of pseudocryptic species in the Eucyclops speratus complex (Copepoda, Cyclopoida) with a description of a new species by Aleksandr Novikov, Dayana Sharafutdinova, Ekaterina Abramova, Tatyana Mayor, Elena Chertoprud

“…Eucyclops speratus (Lilljeborg, 1901) (Copepoda, Cyclopoida, Cyclopidae) was studied using various methods. Molecular genetic methods (comparison of COI and ITSn molecular markers) have shown that this species represents a species complex, and the following methods were used to search for differences between the species: analysis of qualitative and quantitative characters, linear morphometrics, landmark-based geometric morphometrics, and integumental pore pattern of the cephalothorax. …”
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