Species classification of Tabanus (Diptera: Tabanidae) in Western Thailand: Integrating DNA barcoding and modern morphometrics by Tanasak Changbunjong, Thekhawet Weluwanarak, Sedthapong Laojun, Tanawat Chaiphongpachara

“…To enhance the reliability of species identification, DNA barcoding was employed to analyze 30 cytochrome c oxidase subunit 1 (cox1) gene sequences from 15 horse fly species, which were then compared with sequences in the GenBank and BOLD databases. Most cox1 sequences aligned with existing data, with similarity percentages ranging from 96% to 100%. …”
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Complex bacterial diversity of Guaymas Basin hydrothermal sediments revealed by synthetic long-read sequencing (LoopSeq) by John E. Hinkle, John E. Hinkle, Jeffrey P. Chanton, Molly A. Moynihan, Molly A. Moynihan, S. Emil Ruff, S. Emil Ruff, Andreas Teske

“…In general, LoopSeq-derived 16S rRNA gene sequences aligned consistently with reference sequences in GenBank; however, chimeras were prevalent in sequences as affiliated with the thermophilic Candidatus Desulfofervidus and Thermodesulfobacterium, and in smaller numbers within the sulfur-oxidizing family Beggiatoaceae. …”
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Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes by Archimède Mushagalusa Mulega, Arthur F. Boom, Imane Rahmouni, Hocein Bazairi, Abdelaziz Benhoussa, Maarten P. M. Vanhove, Maarten Van Steenberge

“…We identified 11 species using morphology, compared DNA sequences with those available on GenBank, and employed Automatic Barcode Gap Discovery (ABGD) and phylogenetic approaches. …”
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Massive subcutaneous filariosis by Filaria martis in beech marten (Martes foina) in Italy by Giovanni Sgroi, Ranju Ravindran Santhakumar Manoj, Ettore Napoli, Nicola D'Alessio, Maria Gabriella Lucibelli, Claudio de Martinis, Esterina De Carlo, Peyman Khademi, Alireza Sazmand, Vincenzo Veneziano

“…All the filarioids were identified as Filaria martis via morphology and Sanger sequencing of the cytochrome c oxidase subunit I (cox1) gene that revealed a single sequence type (GenBank accession number PQ034642) having 100 % nucleotide identity with those available in the literature. …”
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A model of the artificial metastasis of human epidermoid carcinoma A431 in nude mice for examination of the oncolytic activity of vaccinia virus by G. V. Kochneva, A. A. Grazhdantseva, G. F. Sivolobova, A. V. Tkacheva, A. N. Shvalov, A. Yu. Unusova, E. I. Ryabchikova, S. V. Netesov

“…We used the attenuated recombinant vaccinia virus (VACV) VVdGF-GFP2 of the L-IVP strain (GenBank accession number KP233807), with deletion of two virulence genes: the virus growth factor and thymidine kinase, with the gene for the green fluorescent protein (GFP2) inserted in an area of the latter. …”
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Distribution and species composition of potato viruses in the Novosibirsk region by V. S. Maslennikova, M. B. Pykhtina, K. A. Tabanyukhov, E. V. Shelikhova, K. I. Mosalev, A. V. Katokhin, A. A. Bondar, A. B. Beklemishev, M. I. Voevoda

“…Phylogenetic analysis of the studied sequences of NR isolates was carried out with a set of sequences of reference strains 261-4, Eu-N, N:O, NE-11, NTNa, NTNb, N-Wi, O, O5, SYR_I, SYR_II and SYR_III retrieved from GenBank. As a result of phylogenetic analysis, it was established that NR viral samples fell into two groups of strains: group 1, which also includes isolates of the reference strains 261-4/SYR_III, and group 2, NTNa. …”
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Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology by A. V. Bryanskaya, Y. E. Uvarova, A. S. Rozanov, N. M. Slynko, V. N. Shlyakhtun, K. V. Starostin, E. A. Demidov, E. V. Lazareva, O. P. Taran, S. E. Peltek

“…The resulting DNA sequences of the strains are deposited in the GenBank. The chemotaxonomic characteristics of strains have been determined. …”
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我国东北地区鼩鼱属物种的系统发生地位——基于线粒体COⅠ和16S rRNA分析 by 李金旭 1# 李博琦 1# 赵鑫旭 1 姜雪婷 1 张玉红 1 张隽晟 1 蔡赫 1 田新民 1, 2 金志民 1, 2 刘铸 1

“…鼩鼱属（Sorex）动物分布广泛且与人类接触密切，但其较难捕获导致其系统分化关系并不明确，因此利用线粒体COⅠ和16S rRNA对采集自东北地区的9个鼩鼱属物种，以及从GenBank上下载的8种鼩鼱属的COⅠ序列和13种鼩鼱属的16S rRNA序列进行系统分化分析，计算遗传距离并构建系统进化树，探讨我国东北地区鼩鼱属的系统分化和分类学问题，进而明确其在鼩鼱属的系统发生地位。…”
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Molecular genetic detection and differentiation of <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i>, bacterial leaf streak agents of rice by M. L. Koroleva, S. A. Blinova, A. A. Shvartsev, V. E. Kurochkin, Ya. I. Alekseev

“…They allowed identifying all the strains the sequences of which had been loaded in the GenBank NCBI Nucleotide database before November 11, 2021. …”
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基于泛基因组学和消减蛋白质组学挖掘新型抗诺卡氏菌药物靶点 by 黎尔彤, 苏雅琳, 刘文彬, 金小宝

“…近年来发现诺卡氏菌对常用药物已产生耐药性，因此，挖掘新抗诺卡氏菌药物靶点、开发新药物具有迫切需求。方法取GenBank数据库中31株诺卡氏菌的全基因组序列，利用BPGA进行泛基因组学分析，利用消减蛋白组学筛选药物靶点。…”
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Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi by Meng Xinan, Ou Yonglin, Jiang Wen, Guo Yaqiong, Xiao Lihua, Feng Yaoyu, Li Na

“…In this study, comparison of a clinical genome sequence (C44566) with the whole genome sequence of an E. bieneusi isolate (H348) in GenBank led to the selection of the hypothetical protein 1 (hp1) and tubulin 1 (tub1) loci. …”
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D型肉毒毒素灭鼠剂对棕背雪雀危害机理的试验研究 by 李生庆, 李淑萍, 胡国元, 雷萌桐, 韩生义, 刘成录, 王林业

“…结果显示:棕背雪雀对D型肉毒毒素的敏感性介于高原鼢鼠与青海田鼠之间,LD₅₀为2.71万～3.16万MLD/kg(95%可信限为1.69万～4.88万MLD/kg); 大白鼠胃肠内容物对D型肉毒毒素的破坏强度明显高于高原鼠兔及棕背雪雀; 在基因编码区序列推导的氨基酸序列水平上,棕背雪雀体内的VAMP1氨基酸序列(GenBank No.GQ905710)与昆明系小鼠完全相同,VAMP1氨基酸序列在第48号氨基酸位点(为48M型,而非48Ⅰ型)属于D型肉毒毒素敏感型氨基酸,表明棕背雪雀对D型肉毒毒素具有一定的敏感性,在现地鼠害防治中应精准控制鼠药投放剂量,建议采取药物警戒色趋避鸟类食毒饵。…”
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我国华南中麝鼩分子系统地理学分析 by 栾天琪, 郭秋颖, 高艺, 韩美凤, 张春凤, 陈欢, 蔡赫, 金志民, 张隽晟, 刘铸

“…为了明确我国华南中麝鼩的分子系统地理格局，为其分类学提供进一步分子生物学证据，测定了我国贵州华南中麝鼩20个样本的<italic>Cyt b</italic>基因序列，并结合GenBank下载的19个华南中麝鼩<italic>Cyt b</italic>基因序列进行一并分析。…”
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Immunoinformatics Approach for Multiepitope Vaccine Prediction from H, M, F, and N Proteins of Peste des Petits Ruminants Virus by Bothina B. M. Gaafar, Sumaia A. Ali, Khoubieb Ali Abd-elrahman, Yassir A. Almofti

“…The sequences of the immunogenic proteins were retrieved from GenBank of the National Center for Biotechnology Information (NCBI). …”
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蚊虫细胞色素P450 基因系列研究 by 黄炯烈, 周国理, 吴　瑜, 葛春喜, 王　玲

“…报告中山医科大学寄生虫学教研室医学昆虫学室近5 年来有关蚊虫细胞色素P450 基因(CYP450)系列研究 结果, 包括:①通过简并引物PCR 分别从致倦库蚊、白纹伊蚊及中华按蚊中获得3 个、2 个、2 个CYP4 家族成员基因片段;通 过简并引物PCR 及RT-PCR 从白纹伊蚊中获得16 个CYP6 家族成员基因片段;②利用cDNA 末端快速扩增(RACE)法获得 了白纹伊蚊CYP 6 家族3 个新的全长cDNA 序列及7 个超过其全长一半以上的cDNA 序列。GenBank accession number 分别 为AF283836-AF283838(全长cDNA 序列)和AF284782-AF284783(非全长cDNA 序列), 被国际P450 命名委员会正式命名为 CYP 6N3v1-v3(全长cDNA 序列)和CYP 6N3v4 、CYP 6N4v1-v6, 并证实这些新基因同时存在于白纹伊蚊溴氰菊酯抗性株及 敏感株中;③运用基因步移方法从白纹伊蚊溴氰菊酯抗性株中扩增出CYP 6N3 上游3 076 bp 调控序列;④对白纹伊蚊 CYP 6N3、CYP 6N4 非翻译区序列功能分析显示:昆虫CYP450 与其它真核m RNA 翻译起始及终止机制相似, 但具有多态性 的特点;⑤对白纹伊蚊CYP 6N3 上游启动子区序列特征与功能分析显示:蚊虫中CYP450 转录起始存在着复杂性;⑥证实蚊 虫中CYP 6 存在多样性, 并分析讨论了此种多样性形成的可能机制;⑦分析讨论了蚊虫细胞色素P450 CYP 6 家族分子进化 机制;⑧阐明了下一步研究的目标。…”
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Optimization, characterization and biosafety of oregano, rosemary and mint oil mixture against Penicillium digitatum in citrus using L-optimal mixture design by Rahaf Khaled, Sara Mohamed, Amira Mohamed, Aya Khairy, Hesham Elhariry, Ashraf Bakry, Neima K. Elsenosy, Naglaa Ebeed, Salem S. Salem, Thanaa ElNoby, Samah H. Abu-Hussien

“…P. digitatum NPAGRASU 2024 was isolated and identified as the most abundant species (60.2%) from infected oranges. It was deposited in GenBank with gene accession number PP930644 and deposited in MIRCEN culture collection as EMCC 358874. …”
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In silico development of a multi-epitope-based vaccine against Burkholderia cepacia complex using reverse vaccinology by Donya Ghorbani, Donya Ghorbani, Masoumeh Beig, Masoumeh Beig, Narjes Noori Goodarzi, Mohammad Sholeh, Mohammad Sholeh, Behzad Shahbazi, Behzad Shahbazi, Yaser Moghaddam, Farzad Badmasti

“…BackgroundMultidrug-resistant Burkholderia cenocepacia and Burkholderia multivorans have emerged as significant pathogens, particularly in patients with cystic fibrosis (CF) and chronic granulomatous disease (CGD).ObjectiveGiven the absence of approved vaccines, this study aimed to identify potential vaccine candidates against these pathogens.MethodsThe complete genomes of B. cenocepacia and B. multivorans were retrieved from the GenBank. Surface-exposed proteins that were antigenic, non-allergenic, and non-homologous to human proteins were selected for further analysis. …”
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Genome-Wide Genomic Analysis and Evolutionary Insights into Bovine Coronavirus Strains in Southwest China by Qingqing Li, Huili Bai, Yan Pan, Yuying Liao, Zhe Pei, Cuilan Wu, Chunxia Ma, Zhongwei Chen, Changting Li, Yu Gong, Jing Liu, Yangyan Yin, Ling Teng, Leping Wang, Ezhen Zhang, Tianchao Wei, Hao Peng

“…To elucidate the genetic evolution and characterisation of the prevalent strains, the results of BCoV sequences were compared in GenBank, with a focus on genetic evolution, mutation, and recombination patterns. …”
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Safety evaluation and whole genome sequencing for revealing the ability of Penicillium oxalicum WX-209 to safely and effectively degrade citrus segments by Xiao Hu, Yujiao Qian, Zhipeng Gao, Gaoyang Li, Fuhua Fu, Jiajing Guo, Yang Shan

“…Data availability: The whole genome sequencing data in this study can be found in GenBank database of NCBI website with the accession number PRJNA 715321. …”
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<i>Salmonella enterica</i> serovar Schwarzengrund: Distribution, Virulence, and Antimicrobial Resistance by Monique A. Felix, Jing Han, Bijay K. Khajanchi, Yasser M. Sanad, Shaohua Zhao, Steven L. Foley

“…Data were obtained from GenBank and analyzed for AMR genes using AMRFinder. …”
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