Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH by Biji Shibulal, Saif N. Al-Bahry, Yahya M. Al-Wahaibi, Abdulkadir E. Elshafie, Ali S. Al-Bemani, Sanket J. Joshi

“…The 16S rDNA sequencing of cultivable spore-forming bacteria identified the hydrocarbon utilizing bacteria as Bacillus and Paenibacillus sp. and the nucleotide sequences were submitted to NCBI GenBank under accession numbers KP119097–KP119115. …”
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Phylogenetic Analysis and Antibiotics Resistance of Listeria Monocytogenes Contaminating Chicken Meat in Surabaya, Indonesia by Eduardus Bimo Aksono, Katty Hendriana Priscilla Riwu, A. T. Soelih Estoepangestie, Herinda Pertiwi

“…Some methods were used for the identification procedures, such as biochemical and morphological tests, antibiotic resistance test, PCR, and sequencing; also a phylogenetic analysis was conducted by a neighbor-joining analysis using Genetix Mac ver 8.0 with hlyA genes of Listeria monocytogenes recorded in GenBank, such as Lineage I (KC808543), Lineage II (AY229462, AY229346, AY229499, and AY229404), Lineage III (KJ504139, HQ686043, KJ504116, and DQ988349), and Lineage IV (EU840690, EF030606). …”
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Bacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in <i>Pseu... by Abirami Karthikeyan, Manoj Kumar Thirugnanasambantham, Fazlurrahman Khan, Arun Kumar Mani

“…The potent isolated <i>Bacillus cereus</i> SASAK, identified via 16S rRNA sequencing and deposited in GenBank under accession number MH885570, facilitated the microbial-mediated synthesis of ZnO nanoparticles and silver-doped ZnO NCs. …”
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Prevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of Blastocystis spp. in Anatolian Water Buffalo (Bubalus bubalis) in Van, Türkiye by Adnan Ayan, Burcak Aslan Celik, Ozgur Yasar Celik, Ali Bilgin Yilmaz, Ozlem Orunc Kilinc, Ozge Oktay Ayan

“…., amplicons from buffalo faeces were sequenced and then deposited in GenBank (OR576949.1, OR576950.1, OR576970.1, OR576971.1, OR577019.1, PP837943.1, PP837940.1, PP837939.1, PP837604.1, PP837937.1, PP837934.1, PP837601.1, PP837936.1 and PP837603.1). …”
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Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress by M. Subbulakshmi, Sugapriya Dhanasekaran, S. Abirami, M. Kannan, R. Palaniappan, Divya Venugopal

“…This study identified a unique isoform of 18S rRNA gene (864 bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and flavonoid fractions with retention factor (Rf) values of 0.955 and 0.927 respectively. …”
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黑熊苦味受体Tas2R2基因的分子克隆与序列分析 by 钟 源 潘 阳 侯文海 姚永芳 徐怀亮 张栓玲

“…实验采用PCR和克隆测序方法首次从黑熊基因组中获得一全长为1 019 bp的苦味受体Tas2R2基因DNA序列(GenBank登录号:JQ239035)。该序列含有完整的1个外显子(无内含子),大小为915 bp,编码304个氨基酸残基。…”
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<i>Solanum nigrum</i> L. is a potato spindle tuber viroid reservoir by N. V. Mironenko, A. V. Khiutti, E. I. Kyrova, N. M. Lashina, O. S. Afanasenko

“…The amplification products were cloned and sequenced, and the nucleotide sequences were deposited in the GenBank database.Results. RT-PCR showed that 80–100% of plants were infected with PSTVd. …”
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Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity. by Mostafa Omidian, Zohreh Mostafavi-Pour, Marzieh Asadi, Meysam Sharifdini, Navid Nezafat, Ali Pouryousef, Amir Savardashtaki, Mortaza Taheri-Anganeh, Fattaneh Mikaeili, Bahador Sarkari

“…<h4>Methodology/principal findings</h4>The coding sequences for SsIR and Ss1a were selected from GenBank and were gene-optimized. Using bioinformatics analysis, the regions with the highest antigenicity that did not overlap with other parasite antigens were selected. …”
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A study of macroinvertebrate communities in Bolshiye Koty Bay of Lake Baikal using DNA metabarcoding by L. S. Kravtsova, T. E. Peretolchina, T. I. Triboy, I. A. Nebesnykh, A. E. Tupikin, M. R. Kabilov

“…At a high level of resolution (with homology with GenBank reference sequences ≥ 95 %, coverage ≥ 90 %), 46 taxa of macroinvertebrates comprising three communities were registered: one dominated by molluscs (Choanomphalus conf. maacki) and two dominated by chironomids (Orthocladius grega rius Linev., Sergentia baicalensis Tshern.). …”
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Monkeypox: epidemiological situation, diagnostics, prevention, new challenges and problems of our time by Belyakova E.N., Shipulin G.A.

“…The review used publications presented in the main international medical information databases PubMed, Web of Science, Embase, Elibrary, as well as data from WHO, Centers for Disease Control and Prevention (CDC USA, ECDC, Africa CDC), NCBI GenBank and GISAID. An epidemiological method was used to analyze the publications.…”
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宁夏野生岩羊PPRV全基因组测定与系统进化分析 by 阚龙飞, 孟宪踊, 闫飞虎, 李元果, 王铁成, 徐钰, 初冬, 王卫东, 李岳城, 赵永坤, 高玉伟, 冯娜, 夏咸柱

“…为探究宁夏野生岩羊（<italic>Pseudois nayaur</italic>）小反刍兽疫病毒（Peste des petits ruminants virus，PPRV）的分子遗传特征，根据GenBank公布的PPRV基因组序列设计并合成13对全基因组扩增引物，通过RT-PCR和DNA测序及拼接获取毒株（wild-bharal/China-NXYH/2021株）全基因组序列，借助DNASTAR和MEGA 7.0软件进行序列分析。…”
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Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens by Amira M. Helal, Osama Mahana, Ali Zanaty, Karim Selim, Mohamed. E. Taha, Mostafa M. Saleh, Mohamed I. Hassan, Mona S. Abdallah

“…The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. …”
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Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles by Abebe Bizuye, Lashitew Gedamu, Christine Bii, Erastus Gatebe, Naomi Maina

“…KDT32 and KGT32 isolates were identified as genus Streptomyces and their 16S rRNA gene sequences were deposited in the GenBank database with MH301089 and MH301090 accession numbers, respectively. …”
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Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia by Adyatma Irawan Santosa, Adenisa Hanifah Irbati, Geby Nandita Cahyaning Pratiwi, Antama Surwadinata, Putri Laeshita, Krisnanda Surya Dharma, Reko Saputra Jaya, Annisa Lisa Andriyani, Chilya Qurrota A'yun, Chusnu Naura Syifa Fatika

“…The 552 bp partial AV1 gene sequences of the three isolates were given NCBI GenBank acc nos. OR924278-80. PepYLCIV OR924279 and OR924280 formed a subgroup with LC542629 from Bali in the phylogenetic tree constructed using MEGA11, and shared 95.8 – 96.7% identities at nucleotide (nt) and 98.4 – 99.5% at amino acid (aa) levels according to Sequence Demarcation Tool v1.2 software. …”
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Insights into incompatible plasmids in multidrug-resistant Raoultella superbugs by Jiao Feng, Changxin Wu, Dongsheng Zhou, Lingfei Hu, Kai Mu, Zhe Yin

“…This study aims to elucidate the genomic features of novel incompatible plasmids in MDR Raoultella by presenting 13 complete plasmid sequences from four isolates, along with an analysis of 16 related plasmids from GenBank. These 29 plasmids were classified into five distinct groups: IncFII single-replicon plasmids, dual-replicon plasmids containing the IncFII replicon, IncHI plasmids, IncR plasmids, and IncX8 plasmids. …”
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ANALYSIS OF GENETIC CHARACTERISTICS OF INFLUENZA VIRUS A/CHICKEN/CHELYABINSK/30/2019 H9N2 ISOLATED IN CHELYABINSK OBLAST by N. G. Zinyakov, Щю S. Osipova, P. B. Akshalova, V. Yu. Sosipatorova, A. V. Andriyasov, D. B. Andreychuk, I. A. Chvala

“…The comparative analysis of all genomic segments using the GenBank database revealed a close relationship (over 99%) between the A/chicken/Chelyabinsk/30/2019 H9N2 virus and the A/H9 infl uenza virus isolates circulating in Israel in 2006–2012. …”
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Improving natural red pigment production by Streptomyces phaeolivaceus strain GH27 for functionalization of textiles with in silico ADME prediction by Gehad H. El Sayed, Mohamed Fadel, Rasha Fouad, Hend M. Ahmed, Ahmed A. Hamed

“…Abstract The red pigment was recovered from the S. phaeolivaceus GH27 isolate, which was molecularly identified using 16S rRNA gene sequencing and submitted to GenBank as OQ145635.1. The ideal growth conditions included 1% (w/v) starch, diammonium citrate, dibasic sodium phosphate, 5% (v/v) inoculum, pH 8, a rotation speed of 150 rpm, a temperature of 37 °C, and an incubation period of 9 days. …”
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人血管内皮生长因子165慢病毒载体的构建及其抗放射所致细胞凋亡的作用

“…【目的】 构建人血管内皮生长因子165(hVEGF165)慢病毒表达载体pCDH-CMV-MCS-EF1-copGFPhVEGF165,并转染至HT22 细胞,并进一步观察其对放射线损伤所致的细胞凋亡的保护作用【方法】 PCR扩增hVEGF165基因,克隆到pCDH-CMV-MCS-EF1-copGFP载体;通过酶切电泳,菌落PCR初步筛选和测序鉴定构建的pCDH-CMV-MCS-EF-GFP1-hVEGF165重组载体;采用磷酸钙共转染法转染293T细胞包装制备慢病毒液,并感染HT22细胞,采用实时RT-PCR及Western Blot检测hVEGF165基因在HT22细胞中的表达情况进一步对单纯HT22细胞空质粒转染组以及hVEGF165转染组HT22细胞进行0510 Gy的X线照射,流式细胞仪检测细胞凋亡情况【结果】 hVEGF165基因片段重组到pCDH-CMV-MCS-EF1-copGFP载体,酶切后电泳结果显示均能得到与理论大小相符的片段,测序结果与GenBank序列完全一致,转染HT22后hVEGF mRNA及蛋白表达水平明显增高而且同各对照组相比,hVEGF165可以降低细胞放疗后的凋亡率【结论】 pCDH-CMV-MCS-EF1-copGFP-hVEGF165 慢病毒表达载体构建成功,其转染HT22细胞后可获得高水平hVEGF165 mRNA和蛋白的表达,hVEGF165具有抗放射所致细胞凋亡的作用…”
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Effect of NAM-1 genes on the protein content in grain and productivity indices in common wheat lines with foreign genetic material introgressions in the conditions of Belarus by O. A. Orlovskaya, S. I. Vakula, K. K. Yatsevich, L. V. Khotyleva, A. V. Kilchevsky

“…Full-length NAM-A1 nucleotide sequences of T. dicoccoides k-5199, T. dicoccum k-45926, T. kiharae, and T. spelta k-1731 accessions were established and registered with the international molecular database GenBank. Six combinations of NAM-A1/B1 alleles were identified in the accessions studied and their frequency of occurrence varied from 40 to 3 %. …”
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Molecular Detection of Bartonella spp. in China and St. Kitts by Ke Huang, Patrick John Kelly, Jilei Zhang, Yi Yang, Weiguo Liu, Anwar Kalalah, Chengming Wang

“…By comparing highly conserved 16S rRNA sequences of the better-recognized Bartonella spp. on GenBank, we selected primers and probes for a genus-specific pan-Bartonella FRET-qPCR. …”
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