Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish, by Mohammad Nazrul Islam, Shirin Sultana, Md. Jobaidul Alam

“…The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).…”
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细菌-酵母穿梭表达质粒pGBKT7-LMP1 的构建与鉴定 by 刘　鹏, 马涧泉

“…【结 果】限制性内切酶酶切和DNA 测序分析证实RT-PCR 获得的LMP1 cDNA 片段与GenBank 中的数据完全吻合;LMP1 准确 克隆入pGBKT7 的多克隆位点, 未改变读码框架。…”
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Plant Growth Promoting of Endophytic Sporosarcina aquimarina SjAM16103 Isolated from the Pneumatophores of Avicennia marina L. by S. Rylo Sona Janarthine, P. Eganathan

“…Nucleotide sequence of this strain has been deposited in the GenBank under accession number GU930359. This endophytic bacterium produced 2.37 μMol/mL of indole acetic acid and siderophore as it metabolites. …”
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H6N1亚型禽流感病毒A/Mallard/Heilongjiang/301/2007非结构蛋白基因全序列扩增及分析 by 李晓冰, 华育平, 张进

“…通过设计特异性引物对其非结构蛋白基因（NS）进行了全序列的扩增,并在GenBank中的序列进行比较,发现A/Mallard/Heilongjiang/301/2007属于欧亚系禽流感病毒,此株病毒NS基因与duck/Hokkaido/Vac-1/ 2004 H5N1株NS基因同源率高达98.5%,可能与北海道地区禽流感病毒具有共同起源。…”
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鹤类COI条码变异研究 by 符 渊 白素英 田秀华

“…世界现存15种鹤,该研究采集了7种鹤的样本,获得21条鹤类COI条码序列(695 bp),从GenBank数据库下载41条COI序列,最终获得62条长为620 bp的COI序列用于分析。分析获得25个单倍型,其中包括5个新发现单倍型。…”
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日本血吸虫表达基因 EST 测定及同源性分析 by 吴忠道, 余新炳, 李　焱, 郑亦男, 李晖婷, 周俊梅

“…【结果】完成了 160 个 S j 表达基因EST 序列测定, 获得了126 个可用于分析的 EST, 并在GenBank 登录。10 个为 S j 已知编码基因, 其中3 个为 S j 抗原基因全长 cDNA 。…”
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First microscopic and molecular identification of Cryptosporidium spp. in fat sand rats (Psammomys obesus) in Egypt and their potential zoonotic implications by Sara Abdel-Aal Mohamed, Fatma A. S. Anwar, Ahmed Gareh, Marwa M. I. Ghallab, Remigio Martínez, Asmaa Aboelabbas Gouda, Fatemah Enad Alajmi, Hind Alzaylaee, Ignacio García-Bocanegra, Ignacio García-Bocanegra, Ehab Kotb Elmahallawy, Ehab Kotb Elmahallawy

“…A subset of these positive samples by PCR was subjected to sequencing, with the resulting sequences deposited in GenBank™ and analyzed through phylogenetic methods.ResultsConventional microscopy revealed that 46.7% (70/150; 95% CI: 38.7–54.6) of the analyzed stool samples contained structures consistent with Cryptosporidium oocysts. …”
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Distribution and genetic diversity of three apple viruses in Belarus by P. V. Kuzmitskaya, O. Yu. Urbanovich

“…Estimates of selective pressure on the genome fragments of ACLSV, ASPV and ASGV obtained in this study as well as homologous sequences from the GenBank database indicate negative selection, except for one codon in the sequence of the ACLSV coat protein, which is under positive selection. …”
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Specific PCR Primers Based on Cytochrome-b Gene for Molecular Detection of Javan Mongoose (Herpestes javanicus) by Sena Adi Subrata, Subeno Subeno, Atus Syahbudin

“…The DNA sequences were of 19 Javan carnivore collected from GenBank (NCBI). PCR primers were designed and tested using both in-silico and in-vitro techniques. …”
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华南虎源血巴尔通体的PCR检测 by 陈武, 苏力, 彭仕明

“…2对引物分别扩增出1452 bp和170 bp的rDNA序列,1452 bp序列与GenBank^TM发表的3种猫血巴尔通体序列相似性分别为99.3%、91.7%和79.5%,而170 bp序列与序列AM748929的相似性为100%。…”
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Correction to: Ophiostomatoid fungi associated with Ips subelongatus, including eight new species from northeastern China by Zheng Wang, Ya Liu, Huimin Wang, Xianjing Meng, Xuewei Liu, Cony Decock, Xingyao Zhang, Quan Lu

“…Following publication of the original article [1], the authors reported that Table 1 contained some errors in the GenBank number of BT gene. The correct Table 1 is presented below.…”
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First report of wilt disease in cashew (Anacardium occidentale L.) caused by Fusarium decemcellulare in Kerala, India by A. V. Meera Manjusha, P. K. Laya, Amal Premachandran, M. Veena

“…The culture of the fungus has been deposited with the National Fungal Culture Collection of India at Agharkar Research Institute, Pune, India with accession number NFCCI 4801, and sequence of the fungus was deposited at GenBank of NCBI with accession number OP942472.…”
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广东人AGTR2突变热区的SNPs位点分析

“…【结果】 发现5个SNPs位点:G300663A,A300672T,A300818T,G301404T和A301410G,除A301410G与Gen-Bank dbSNP数据库中的位点(rs5194)相同外,其余4个位点为新发现的SNPs位点?…”
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PCR技术在麦哲伦企鹅性别鉴定上的应用 by 王玉英 1# 徐翊轩 1# 靳鹏 1 王云忠 1 黄大鹏 1 李超 2

“…以青岛水族馆7只麦哲伦企鹅的新鲜血液为对象提取基因组DNA，根据基因组数据设计性别特异引物P2/P8，对其CHD基因进行PCR扩增，回收扩增的目的片段进行测序，获得这些麦哲伦企鹅的性别相关基因序列。将这7只麦哲伦企鹅的CHD基因序列与GenBank上公布的短趾雕的CHD基因序列进行同源性比对分析，建立遗传进化树，初步确定编号为1572、1573、1574、1575、36的麦哲伦企鹅鉴定为雄性，编号29和1577号麦哲伦企鹅为雌性。…”
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小熊猫源犬瘟热病毒DNA疫苗构建及其在真核细胞的表达 by 张晓明 陈 武 彭仕明

“…根据Gen Bank中发表的犬瘟热病毒(CDV)的基因组序列设计了H和F基因的2对引物,通过RT-PCR扩增出从发病小熊猫分离的CDV的H和F基因,并将其克隆入到18T-simple载体上进行测序。…”
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人 CD134L 基因原核表达载体的构建、鉴定及序列分析 by 张　强, 张春艳, 宁　波, 陈琼玲

“…【结果】人CD134L cDNA 已正确克隆到原核表达载体 pGEX-4T-1 中,测序结果表明中国人CD134L cDNA 与GenBank 国外报告的完全 一致。【结论】本研究成功构建人CD134L 的原核表达载体,为下一步进行人 CD134L 与自身免疫性疾病的关系奠定基础。…”
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Purification and Optimization of Extracellular Lipase from a Novel Strain Kocuria flava Y4 by Adyasa Barik, Sudip Kumar Sen, Geetanjali Rajhans, Sangeeta Raut

“…In this study, the extracellular lipase activity of Kocuria flava Y4 (GenBank accession no. MT773277), isolated from Dioscorea villosa during our previous study, was regulated by different physicochemical parameters, such as pH, temperature, shaking speed, and incubation time. …”
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Genetic Diversity in Passiflora Species Assessed by Morphological and ITS Sequence Analysis by Shiamala Devi Ramaiya, Japar Sidik Bujang, Muta Harah Zakaria

“…The final dataset of aligned sequences from nine studied Passiflora accessions and 30 other individuals obtained from GenBank database (NCBI) yielded one most parsimonious tree with two strongly supported clades. …”
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浙江金华发现红耳巢鼠 by 苏梓诚, 问青青, 章铁睿, 龚宴安, 洪佳, 庄前进, 魏濮瑞, 庄家尧, 黄松

“…基于细胞色素<italic>b</italic>基因（<italic>Cyt b</italic>）序列（972 bp）构建最大似然树，显示该标本与从GenBank下载的红耳巢鼠同源序列构成单系群（支持率为100%）。基于以上结果，确定该标本为红耳巢鼠，是浙江省新纪录种。…”
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Application of internal transcribed spacer (ITS) sequences for identifying Anoectochilus setaceus Blume in Thanh Hoa, Vietnam by B. B. Thinh, L. D. Chac, L. T.M. Thu

“…The findings identified the samples as the same species and showed 99% similarity to the ITS gene sequence of A. roxburghii (Wall.) Lindl. published in GenBank, GQ328774. This study also demonstrates that the method employing internal transcribed spacer (ITS) sequences is an effective tool to identify A. setaceus taxa.…”
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