广州地区HCMV临床病毒株UL137 基因的结构 特征与多态性

“…对3株临床分离株进行HCMV UL137基因全序列PCR扩增,PCR产物纯化后进行基因克隆,构建HCMV UL137-pMD18-T重组质粒;基因测序;将HCMV UL137基因序列呈报GenBank,并进行基因生物信息学分析?【结果】成功从广州地区新生儿感染HCMV患儿体内分离3株HCMV临床病毒株?…”
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Analysis of the IGS rRNA Region and Applicability for Leishmania (V.) braziliensis Characterization by Tayná C. de Goes, Rayana C. S. de Morais, Maria G. N. de Melo, Rômulo P. e Silva, Antônio M. Rezende, Milena de Paiva-Cavalcanti

“…The sequences obtained were aligned and analyzed for size and similarity, as well as deposited in GenBank. Characteristics of the repetitive elements (IGSRE) present in the IGS rRNA were also verified. …”
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The InBIO Barcoding Initiative Database: contribution to the knowledge of DNA barcodes of the vascular plants of north-eastern Portugal by João Queirós, Rodrigo Silva, Catarina J. Pinho, Hélia Vale-Gonçalves, Ricardo Pita, Paulo Alves, Pedro Beja, Joana Paupério, Miguel Porto

“…This is one of the public releases of the IBI database, which provides genetic and distributional data for several taxa.All vouchers are deposited in the Herbarium of the Museum of Natural History and Science of the University of Porto (MHNC-UP) and their DNA barcodes are publicly available in the Barcode of Life Data System (BOLD), NCBI GenBank online databases and International Nucleotide Sequence Database Collaboration (INSDC).…”
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Analyzing cellulolytic bacteria diversity in mangrove ecosystem soil using 16 svedberg ribosomal ribonucleic acid gene by I. Dewiyanti, D. Darmawi, Z.A. Muchlisin, T.Z. Helmi

“…These species exhibited an identity value of 97.77-100 percent when compared to data from GenBank, and B. velezensis was found to have a close relationship with B. amyloliquefaciens at a value of 0.002 percent. …”
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Coproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals by Dina Aboelsoued, Nagwa I. Toaleb, Kadria N. Abdel Megeed

“…Molecular identification of Cryptosporidium samples was performed based on COWP gene, revealing the isolates: GenBank: OQ121955.1, OR029973.1 and PP316107.1 which were identical to the C. parvum and GenBank: PP316108.1 and OR029972.1 which were identical to C. hominis and C. andersoni, respectively. …”
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Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey by Armagan Erdem Utuk, Fatma Cigdem Piskin

“…Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. …”
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L’enfant de Mezmaiskaya (Caucase) examiné dans une double perspective paléogénétique et paléoanthropologique by Véronique Barriel, Anne-Marie Tillier

“…The palaeogenetic interpretation is examined in the present article based on data available for modern humans (Anderson et al. 1981; HvrBase; http://www.hvrbase.de) and for three Middle Palaeolithic fossils from Germany (Feildhofer 1 and 2 ; Krings et al. 1997; Krings et al. 1999; Schmitz et al. 2002) and Croatia (Vindija-75-G3/h-203; Krings et al. 2000), which are attributed to the Neanderthal line and those of Pan paniscus and the four sub-species of Pan troglodytes (EMBL and GenBank). The question of the phylogenetic status of the Mezmaiskaya child is examined using both molecular analysis and anthropological arguments.…”
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Whole-Genome Sequence of Chelatococcus daeguensis Strain M38T9, Isolated from Ulu Slim Hot Spring in Malaysia by Yi Xian Goh, Kok Gan Chan, Kar Wai Hong

“…The draft genome has been deposited at DDBJ/ENA/GenBank under the BioProject accession number PRJNA668056. …”
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Characterization of the complete genome sequence of the recombinant norovirus GII.P16/GII.4_Sydney_2012 revealed in Russia by E. V. Zhirakovskaia, A. Y. Tikunov, S. N. Sokolov, B. I. Kravchuk, E. I. Krasnova, N. V. Tikunova

“…Before this study, only four complete genome sequences of the Russian GII.P16/GII.3 norovirus strains were available in the GenBank database. In this work, the complete genome sequence of the Russian strain Hu/GII.P16-GII.4/RUS/Novosibirsk/NS16-C38/2016 (GenBank KY210980) was determined. …”
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山东省某地鹦鹉喙羽病病毒的全基因组序列及对SPF鸡胚的致病性探究 by 吴少鹏宋艳孙淑红

“…由于目前尚未发现适合PBFDV繁殖的培养细胞或胚胎，我们尝试使用患病鹦鹉的羽毛研磨液注入7日龄SPF鸡胚的卵黄膜中。结果：分离到3株PBFDV（GenBank登录号：MH279594，MH180298和MH190788），全基因组序列测序后，发现它们彼此具有933%—999%的相似性，并且它们也与GenBank中的一些参考序列具有86.1%—98.8%的相似性。…”
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野鸭源H6N2亚型禽流感NS基因的克隆及序列分析 by 张智明, 李晓冰, 华育平

“…根据GenBank上已发表的H6N2亚型的非结构蛋白基因（NS）序列,设计一对特异性引物,成功从本实验室分离、保存的H6N2禽流感病毒分离株A／Mallard／SanJiang/151／06（H6N2）中克隆到NS基因序列。…”
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Analysis of whole genome sequence of strain "0" of avian infectious laryngotracheitis virus by A. A. Kozlov, N. G. Zinyakov, S. N. Kolosov, N. S. Mudrak, I. A. Chvala

“…The mapped nucleotide sequence has been deposited in the international GenBank database under the accession number KU128407. …”
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Fungal phoenix rising from the ashes? by M.J. Wingfield, M.P.A. Coetzee, P.W. Crous, D. Six, B.D. Wingfield

“…The ITS and IGS-1 regions were sequenced and compared to sequences of Armillaria spp. available on GenBank. Cladograms were generated using ITS sequences to determine the phylogenetic relationship of the isolates with other Armillaria spp. …”
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dataFishing: An efficient Python tool and user-friendly web-form for mining mitochondrial and chloroplast sequences, taxonomic, and biodiversity data by Luan Rabelo, Davidson Sodré, Oscar David Albito Balcázar, Murilo Furtado do Rosário, Aurycéia Jaquelyne Guimarães-Costa, Grazielle Gomes, Iracilda Sampaio, Marcelo Vallinoto

“…NCBI GenBank and BOLD Systems are important databases for biodiversity research, in which the deposited data can be used for various purposes, such as species identification analysis, evolutionary studies, biodiversity monitoring, as well as assessing the effects of possible climate changes on species distributions. …”
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转基因植物表达质粒中SBR 基因的序列分析 by 蒋少云, 凌均

“…【方法】从 E .coli DH5α中提取质粒pROP1, 用PCR 测序试剂盒和DNA 自动测序仪检测P1 片段的序列, 并与GenBank 上的变形链球菌 表面蛋白基因序列比较, 检测其准确性。【结果】测定了P1 片段5′端616 个碱基序列和3′端466 个碱基序列, 其准确性达 98 %。…”
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VARIABILITY OF MITOCHONDRIAL CYTOCHROME OXYDASE SUBUNIT I GENE SEQUENCE IN SPECIES OF THE GENERA AEDES AND OCHLEROTATUS (DIPTERA: CULICIDAE) by N. V. Khrabrova, Yu. V. Andreeva, O. V. Vaulin, S. S. Alekseeva, A. K. Sibataev

“…Sequence variation in the 538-bp fragment from the 5′ end of the COI region was analyzed to test its usefulness in the identification of 15 mosquito species of the genera Aedes and Ochlerotatus (Diptera: Culicidae) from Tomsk and Kemerovo regions and 14 species from GenBank. The divergences between congeneric species averaged 7,8 % (from 0,7 to 13,1 %), whereas those for conspecific individuals averaged 0,57 % (0 to 1,6 %). …”
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Bioadsorptive Removal of the Pollutant Zn(II) from Wastewater by Delftia tsuruhatensis Biomass by Samiah Hamad Al-Mijalli

“…D. tsuruhatensis SA-101 has been assigned the accession number MW629784 in the GenBank database. The optimal pH and reaction contact time for Zn(II) removal by D. tsuruhatensis SA-101 were 6.0 and 30 min, respectively. …”
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野猪甘露聚糖结合凝集素A基因的克隆及其生物信息学分析 by 孙玉刚, 刘玉芬, 王洋, 满朝来, 赵文阁

“…根据GenBank上已发表的猪甘露聚糖结合凝集素A基因序列,设计一对特异性引物,通过RT-PCR法成功地从野猪肝脏中克隆到MBL-A基因序列。…”
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GEOMETRIC PROPERTIES OF EVOLUTIONARY DISTANCES by V. M. Efimov, M. A. Melchakova, V. Yu. Kovaleva

“…The applicability of the EPQ distance is illustrated by the example of a cytochrome b sequence set of 12 rodent species from West Siberia and Altai, taken from the GenBank, and compared with the results of the use of the LogDet-distance.…”
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c-myb 反义RNA 重组载体构建及其包装细胞株的建立 by 马会慧, 姚集鲁, 李　刚, 姚春斓, 陈雪娟, 杨绍基

“…采用DOTAP 法将重组逆转录病毒载体转入包 装细胞, 以NIH3T3 细胞为靶细胞测定产病毒滴度。【结果】序列测定结果与GenBank 中序列一致。c-myb 基因片段已定向 克隆入pDOR。细胞转染表明, 已形成稳定的包装细胞株PA317/ pDOR-my b, 产病毒滴度达5.2 ×104 ～ 9.5 ×104 CFU/m L。 …”
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