First report of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae), biotypes B and Q, in soybean Glycine max (L.) Merrill (Fabales: Fabaceae) in Paraguay by Nancy Noemí Espinoza-Morel, Marta Alicia Fernández-Gamarra, Liliana Noelia Talavera-Stefani, Cinthia Noemí Burgos-Cantoni, Felipe Giménez, Claudia Cabral, Andrés Sanabria-Velazquez

“…Sequencing of the PCR products revealed a 100% match with known sequences for B. tabaci and its biotypes, as confirmed by GenBank BLAST analysis. This research highlights the critical importance of continuously monitoring the population dynamics and geographical distribution of different biotypes. …”
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Detection of CRISPR‒Cas and type I R–M systems in Klebsiella pneumoniae of human and animal origins and their relationship to antibiotic resistance and virulence by Xue Li, Ling Wang, Jinghuan Lin, Yingjuan Gu, Zhihua Liu, Jing Hu

“…In this study, the whole genomes of 520 strains of K. pneumoniae from GenBank, including 325 from humans and 195 from animals, were collected for CRISPR‒Cas systems and type I R-M systems, virulence genes, antibiotic resistance genes, and multilocus sequence typing detection. …”
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First evidence of transovarial transmission of Kyasanur Forest disease virus in Haemaphysalis and Rhipicephalus ticks in the wild by Sarah J. Burthe, Bhimanagoud Kumbar, Stefanie M. Schäfer, Bethan V. Purse, Abi T. Vanak, Natrajan Balakrishnan, Richard Hassall, Subhash L. Hoti, Darshan Narayanaswamy, Santoshkumar Potadar, Mujeeb Rahman, Mohammed Mudassar Chanda

“…Five different tick species were found to have KFDV-positive larvae: Haemaphysalis spinigera, H. bispinosa, Rhipicephalus annulatus, R. microplus and an unidentifiable species of Haemaphysalis (no close match in GenBank). Conclusions Our empirical confirmation of transovarial transmission has important implications for understanding and predicting KFD dynamics, suggesting that ticks may act as a reservoir for KFDV. …”
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Analysis of changes in the genome of the Omsk hemorrhagic fever virus (Flaviviridae: <i>Orthoflavivirus</i>) during laboratory practices for virus preservation by Zhanna S. Tyulko, Artem V. Fadeev, Aleksei G. Vasilenko, Ekaterina A. Gradoboeva, Valerii V. Yakimenko, Andrey B. Komissarov

“…Whole-genome nucleotide sequences of OHF virus strains from the working collection of the laboratory of arboviral infections of the department of natural focal viral infections of the Omsk Research Institute of Natural Focal Infections of Rospotrebnadzor were used in the study, as well as sequences from GenBank. Assessment of adaptive changes in the genome of the OHF virus was carried out using discriminant analysis methods, analyzing the composition and localization of emerging substitutions in viral RNA sequences obtained during the adaptation of viruses to the mouse organism as a result of passaging. …”
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Identification of Differentially Expressed Genes in Metastatic and Non-Metastatic Nasopharyngeal Carcinoma Cells by Suppression Subtractive Hybridization by Xu-Yu Yang, Cai-Ping Ren, Lei Wang, Hui Li, Chun-Jie Jiang, Hong-Bo Zhang, Ming Zhao, Kai-Tai Yao

“…Among these 20 clones, 16 were previously identified genes (flotilin-2, ezrin, pim-3, fli-1, mel, neugrin, znf216, ASB1, raly, UBE2A, keratin6A, TMED7, EIF3S9, FTL, two ribosomal proteins RPL21 and RPL16), two were predicted genes (c9orf74 and MDS006), and two sequences shared no homology with known genes listed in GenBank and may represent novel genes. The proposed functions of the genes identified in this study include cell signal transduction, cell survival, transcription regulation, cell mobility, protein synthesis, and DNA damage repair. …”
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Plastid genome comparison and phylogenetic analyses of the Chinese group of medicinal species and related taxa within Asparagus genus by Pingxuan Xie, Tinglu Wang, Jiawei Tan, Linyuan Fan, Changkun Liu, Hanjing Yan

“…However, accurate authentication of these medicinal species and their phylogenetic relationships with related taxa remains unresolved.MethodsTo identify simple sequence repeats (SSRs) and divergence hotspot regions appropriate for future authentication studies, as well as to infer the phylogenetic relationships among Asparagus species, we employed a plastid genome (plastome) dataset consisting of 25 Asparagus species (21 newly sequenced and four retrieved from GenBank), encompassing 12 Chinese medicinal species, for comparative and phylogenetic analyses.ResultsAll Asparagus plastomes displayed a typical quadripartite structure with sizes ranging from 155,948 bp to 157,128 bp and harbored 114 unique genes (80 protein-coding genes, 30 tRNA genes, and four rRNA genes). …”
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Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties by O. Y. Antonova, N. S. Klimenko, Z. Z. Evdokimova, L. I. Kostina, T. A. Gavrilenko

“…The sequences of the Rpi-sto1- and BLB1 F/R-amplicons were identical in all the genotypes of group ‘C’ and showed respective 99 % and 100 % similarity to the corresponding fragments of the Rpi-sto1 and Rpi-blb1 genes from the GenBank database. Among the genotypes of group ‘C’ various mt-types were detected, and some of them were male fertile.…”
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Comparative Genomic Analysis of <i>Campylobacter</i> Plasmids Identified in Food Isolates by Yiping He, Gretchen Elizabeth Dykes, Siddhartha Kanrar, Yanhong Liu, Nereus W. Gunther, Katrina L. Counihan, Joe Lee, Joseph A. Capobianco

“…Pangenomic analysis of the plasmid assemblies and reference plasmids from GenBank revealed five distinct groups, namely, pTet, pVir, mega plasmids (>80 kb), mid plasmids (~30 kb), and small plasmids (<6 kb). …”
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Global insights into the genome dynamics of Clostridioides difficile associated with antimicrobial resistance, virulence, and genomic adaptations among clonal lineages by Mohammad Sholeh, Mohammad Sholeh, Masoumeh Beig, Masoumeh Beig, Ebrahim Kouhsari, Mahdi Rohani, Mohammad Katouli, Farzad Badmasti

“…This study offers a genomic analysis of C. difficile, focusing on sequence types (STs), global distribution, antibiotic resistance genes, and virulence factors in its chromosomal and plasmid DNA.MethodsA total of 19,711 C. difficile genomes were retrieved from GenBank. Prokka was used for genome annotation, and multi-locus sequence typing (MLST) identified STs. …”
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Growth performance and gut health of Murrah buffalo calves fed with developed novel synbi- otic formulation by Antony J. Thanippilly, Sachin Kumar, Vinay V. Varada, Nitin Tyagi, Ashish K. Samanta

“…Ligilactobacillus salivarius BF17 (NCBI GenBank Accession No-MG966326), a potential probiotic previously isolated from Murrah buffalo calves, was chosen and evaluated for the best prebiotic substrate by screening five different prebiotics (fructo oligosaccharides, mannan oligosaccharides, inulin, corn dextrin, and wheat dextrin). …”
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Specific detection of pigeon parvovirus with TaqMan real-time PCR technology by Cuiteng Chen, Chunhua Zhu, Shuyu Chen, Zhen Chen, Huanru Fu, YuYi Chen, Mengyan Zhang, Wenyu Zhang, Yu Huang, Longfei Cheng, Chunhe Wan

“…The specific primers and probes used were designed on the basis of the NS gene characterization of PiPV downloaded from GenBank. After optimization, the established TaqMan‒PCR assay provides a sensitive, accurate, reliable and cost-effective platform for PiPV detection. …”
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Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of Europ... by Sergey V. Lenshin, Tatyana V. Vishnevskaya, Alexey V. Romashin, Yulia I. Bulycheva, Oleg I. Vyshemirsky, Sophya A. Solovyeva, Asya K. Gitelman, Alexey S. Pazilin, Dmitry K. Lvov, Ben Hu, Zheng-Li Shi, Sergey V. Alkhovsky

“…A novel alphacoronavirus, designated Kudep virus (GenBank acc. # PQ649435), was identified in R. ferrumequinum. …”
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Morphological Characteristics, Mitochondrial Genome, and Evolutionary Insights Into a New Sea Squirt From the Beibu Gulf by Yichuan Zhang, Yuting Qin, Yueying Wu, Liping Liu, Wenguang Zhang, Ling Ding, Xiangpei Ya, Zhiting Wen, Kuaili Feng, Hong Wang, Yujun Wang

“…Most importantly, the mitochondrial genome characteristics of YZ‐2024 effectively and accurately distinguish it from other congeneric species, with a mitochondrial genome size of 14,520 bp (GenBank No. PP067884) and the proportions of bases A, T, G, and C comprising 26.83%, 47.16%, 16.91%, and 9.10%, respectively. …”
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Bees on the run: Nosema spp. (Microsporidia) in Apis mellifera and related products, Italy by Giovanni Sgroi, Luigi Jacopo D’Auria, Luigi Jacopo D’Auria, Maria Gabriella Lucibelli, Andrea Mancusi, Yolande Thérèsa Rose Proroga, Mauro Esposito, Mauro Esposito, Simona Rea, Daniel Signorelli, Federica Gargano, Nicola D’Alessio, Ranju Ravindran Santhakumari Manoj, Peyman Khademi, Giuseppe Rofrano, Giuseppe Rofrano

“…The sequences of N. apis and N. ceranae had 99.5–100% homology with those in the GenBank database. Whereas all sequences of N. apis were identical to each other, four sequences types of N. ceranae characterized by single nucleotide polymorphisms (SNPs) were identified. …”
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Current data on the circulation of the Q fever pathogen in the Republic of Guinea by Ekaterina V. Naidenova, Kirill S. Zakharov, Dmitry A. Agafonov, Mikhail Yu. Kartashov, Aislu M. Senichkina, Erik S. Khalilov, Abdoul B. Ibrahim, Mamadou B. Bah, Ibrahim Nourdine, Nikolai K. Tokarevich, Sanaba Boumbaly, Yusuf Sidime, Svetlana A. Shcherbakova, Vladimir V. Kutyrev

“…For the first time, the complete nucleotide sequence of 16S rRNA of the Q fever pathogen (OQ152497–OQ152500) identified on the territory of Guinea was determined and registered in the GenBank database. Conclusion. Taking into account the high epidemiological significance of Q fever, the study of the specifics of C. burnetii circulation in Guinea remains an urgent task. …”
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L-glutaminase synthesis by Klebsiella pneumoniae (AS KP 23) isolated from clinical strain, and its efficacy against human hepatocellular and breast cancer cell lines by Lina J. Abdel-Hafez, Eman Y. T. Elariny, Asmaa E. Ibrahim, Mahmoud E. F. Abdel-Haliem

“…The 16 S rRNA gene was 100% identical to the sequence from Klebsiella pneumoniae and was submitted to GenBank under accession number OQ703039. Thus, this strain was named Klebsiella pneumoniae AS KP 23. …”
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Optimization and characterization studies of poultry waste valorization for peptone production using a newly Egyptian Bacillus subtilis strain by Hajar Saeed, Anthony Ragaey, Ziad Samy, Viola Ashraf, Aly ElMostafa, Norhan Ahmad, Enjy Bebawy, Nour ElHoda M. Sorour, Salwa M. El-Sayed, Ashraf Bakry, Naglaa Ebeed, Hesham Elhariry, Thanaa El-Noby, Samah H. Abu-Hussien

“…The isolation process yielded the highly potent proteolytic B.subtilis isolate P6, identified through 16S rRNA gene sequencing to share 94% similarity with the B.subtilis strain KEMET024 (GenBank accession number PP694485.1) and deposited in MIRCEN culture collection, Cairo, Egypt as EMCC 998871. …”
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