Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis by Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin

“…Knockdown of IncE caused loss of IncE and its interacting host protein SNX6 at the inclusion membrane, without affecting IncG protein levels. Similarly, IncG knockdown significantly reduced IncG levels and prevented recruitment of its interacting host protein 14-3-3β, without altering IncE protein levels. …”
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CCR2 signaling regulates anti-chlamydia T cell immune responses in the airway. by Shuaini Yang, Jinxi Yu, Xue Dong, Jiajia Zeng, Lu Tan, Hong Zhang, Ruoyuan Sun, Yuqing Tuo, Jing Yang, Chunxiao Wan, Hong Bai

“…CCR2, a member of the G protein-coupled receptor (GPCR) superfamily, is widely expressed on monocytes, macrophages, activated T cells, and other cell types, and plays a critical role in coordinating the immune response to various infections. …”
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Chronic GIPR agonism results in pancreatic islet GIPR functional desensitisation by Iona Davies, Alice E. Adriaenssens, William R. Scott, David Carling, Kevin G. Murphy, James S. Minnion, Stephen R. Bloom, Ben Jones, Tricia M-M. Tan

“…Objectives: There is renewed interest in targeting the glucose-dependent insulinotropic polypeptide receptor (GIPR) for treatment of obesity and type 2 diabetes. G-protein coupled receptor desensitisation is suggested to reduce the long-term efficacy of glucagon-like-peptide 1 receptor (GLP-1R) agonists and may similarly affect the efficacy of GIPR agonists. …”
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Optimization of Protein Hydrolysate from Earthworms (<i>Perionyx excavatus</i>) of Angiotensin-I-Converting Enzyme Inhibitory Activity by One Variable at a Time by Phong Thanh Bui, Truc Anh Hoang, My Thi Thuy Pham, Linh Le Phuong Tran, Oanh Kim Nguyen

“…The results showed that the protein hydrolysate of earthworms gave the highest ACEIA of 85.38 ± 2.31% (at the protein concentration of 2 mg/mL) and IC50 value of 844.64 μg/mL when the earthworms were hydrolyzed with Flavourzyme® 500 MG under optimized conditions such as earthworm:phosphate buffer ratio of 1:6 (w/v), hydrolysis temperature of 50 °C, pH 7, enzyme:substrate ratio of 600 U/g protein and hydrolysis time of 2 h. The membrane further fractionated the hydrolysate, and the < 1 kDa fraction had the highest ACEIA with an IC50 value of 261.94 μg/mL. …”
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A broad spectrum Shigella vaccine based on VirG53 – 353 multiepitope region produced in a cell-free system by Girmay Desalegn, Charlotte Abrahamson, K. Ross Turbyfill, Lucy Pill-Pepe, Leslie Bautista, Chitradevi S. Tamilselvi, Dylan Dunn, Neeraj Kapoor, Barbara Sullinger, Matheo Herrera, Edwin V. Oaks, Jeff Fairman, Marcela F. Pasetti

“…Seeking to refine the vaccine antigenic target and achieve scalable manufacturing compatible with Good Manufacturing Practices, we mapped linear B-cell epitopes spanning the entire VirG protein sequence by probing the immune reactivity of 10-mer peptides (overlapping 4–8 aa) with sera from Shigella-infected rhesus monkeys. …”
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Promoter Polymorphism of RGS2 Gene Is Associated with Change of Blood Pressure in Subjects with Antihypertensive Treatment: The Azelnidipine and Temocapril in Hypertensive Patients... by Ken Sugimoto, Tomohiro Katsuya, Kei Kamide, Tomomi Fujisawa, Izumi Shimaoka, Mitsuru Ohishi, Ryuichi Morishita, Toshio Ogihara, Hiromi Rakugi

“…At eight weeks, BP change showed a significant interaction between the A-638G polymorphism of Regulator of G protein signaling-2 (RGS2) gene and treatment with azelnidipine or temocapril. …”
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Iron-Binding Capacity and Antidiabetic Activity of Baby Clam (<i>Corbiculidae</i> sp.) Meat Protein Hydrolysate by Tam Dinh Le Vo, Thu Huynh, Thuy Thi Le, An Thi Tuong Tran, Bao Chi Vo

“…Under the best condition, the hydrolysates demonstrated the IBC of 1246.20 ± 44.00 µg Fe2+/g protein and α-amylase inhibition activity of 48.33 ± 1.44%, approaching three-quarters of the activity of ethylenediaminetetraacetic acid (EDTA) sodium salt and acarbose, respectively. …”
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Sustained Release of Prostaglandin E2 in Fibroblasts Expressing Ectopically Cyclooxygenase 2 Impairs P2Y-Dependent Ca2+-Mobilization by María Pimentel-Santillana, Paqui G. Través, Raquel Pérez-Sen, Esmerilda G. Delicado, Paloma Martín-Sanz, María Teresa Miras-Portugal, Lisardo Boscá

“…The nucleotide uridine trisphosphate (UTP) released to the extracellular milieu acts as a signaling molecule via activation of specific pyrimidine receptors (P2Y). P2Y receptors are G protein-coupled receptors expressed in many cell types. …”
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Phytocompounds from Indonesia Medicinal Herbs as Potential Apelin Receptor Agonist for Heart Failure Therapy: An In-silico Approach by Muhamad Rizqy Fadhillah, Wawaimuli Arozal, Muhammad Habiburrahman, Somasundaram Arumugam, Heri Wibowo, Suci Widya Primadhani, Aryo Tedjo, Surya Dwira, Nurul Gusti Khatimah

“…The Apelin Receptor (APR), a class A (rhodopsin-like) G-Protein Coupled Receptor (GPCR), is one of the cell membrane receptors that potentially become a specific target of heart failure therapy when activated. …”
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Quality Control of Fetal Wharton&rsquo;s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles by Krishnan I, Ng CY, Kee LT, Ng MH, Law JX, Thangarajah T, Zainuddin AA, Mahmood Z, Rajamanickam S, Subramani B, Lokanathan Y

“…Quantitatively, the protein and particle concentrations revealed that all the sEV preparations were “impure” with < 1.5 × 109 particles/μg protein. Albumin was co-isolated in all the sEV preparations.Conclusion: In short, all characterized and standardized individual and pooled sEV preparations were deemed “impure” due to albumin co-isolation using the TFF method. …”
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MiR-155-Mediated Deregulation of GPER1 Plays an Important Role in the Gender Differences Related to Inflammatory Bowel Disease by Xiaojuan Shao, Jintao Li, Fumin Xu, Dongfeng Chen, Kaijun Liu

“…In IBD, microRNA-155 (miR-155) is upregulated and regulates G protein coupled estrogen receptor (GPER1), which affects the intestinal flora. …”
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Binding of HCN channels to GABAB receptors in dopamine neurons of the VTA limits synaptic inhibition and prevents the development of anxiety by Enrique Pérez-Garci, Kateryna Pysanenko, Giorgio Rizzi, Florian Studer, Daniel Ulrich, Thorsten Fritzius, Simon Früh, Alessandra Porcu, Valérie Besseyrias, Adolf Melichar, Martin Gassmann, Tania Rinaldi Barkat, Rostislav Tureček, Kelly R. Tan, Bernhard Bettler

“…During GABAergic synaptic transmission, G protein-coupled GABAB receptors (GBRs) activate K+ channels that prolong the duration of inhibitory postsynaptic potentials (IPSPs). …”
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Unlocking the Potential of Receptor-Based Approaches in Diabetes Treatment by Mohsina Patwekar, Faheem Patwekar, J. Pavan Kumar, P. Dharani Prasad, Nazia Malik, A. Venkata Badarinath, Prashanth Parupathi, Konatham Teja Kumar Reddy, Selvaraja Elumalai, Zainul Abedeen Ab Samad

“…Pegbelfermin (BMS-986036), a fibroblast growth factor 21 (FGF21) analogue, is being investigated for its ability to regulate glucose and lipid metabolism, and potentially enhance glycemic control and lipid profiles. Additionally, G-protein-coupled receptor (GPCR) agonists and adenosine monophosphate-activated protein kinase (AMPK) activators are emerging as potential medicines to improve insulin sensitivity, glucose uptake, and insulin signaling pathways. …”
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ArfGAP with the SH3 Domain, Ankyrin Repeat and PH Domain 1 Inversely Regulates Programmed Death-Ligand 1 Through Negative Feedback of Phosphorylated Epithelial Growth Factor Recept... by Chiba N, Menju T, Shimazu Y, Toyazaki T, Sumitomo R, Miyamoto H, Tamari S, Nishikawa S, Date H

“…Naohisa Chiba,1 Toshi Menju,1 Yumeta Shimazu,1 Toshiya Toyazaki,1 Ryota Sumitomo,1 Hideaki Miyamoto,1 Shigeyuki Tamari,1,2 Shigeto Nishikawa,1 Hiroshi Date1 1Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan; 2Department of Thoracic Surgery, Shizuoka City Shizuoka Hospital, Shizuoka, JapanCorrespondence: Toshi Menju, Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, 54 Shigoin-Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan, Tel +81-757514975, Email toshimnj@kuhp.kyoto-u.ac.jpBackground: Signaling pathways centered on the G-protein ADP-ribosylation factor 6 (Arf6) and its downstream effector ArfGAP with the SH3 Domain, Ankyrin Repeat and PH Domain 1 (AMAP1) drive cancer invasion, metastasis, and therapy resistance. …”
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Delivery determinants of an Acinetobacter baumannii type VI secretion system bifunctional peptidoglycan hydrolase by Valeriya Bezkorovayna, Brooke K. Hayes, Francesca N. Gillett, Amy Wright, David I. Roper, Marina Harper, Sheena McGowan, John D. Boyce

“…Each effector, Tse15, Tde16, and Tae17, is delivered via a non-covalent interaction with a specific T6SS VgrG protein (VgrG15, VgrG16, and VgrG17, respectively). …”
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Effects of Storage Time on Total Protein and Globulin Concentrations in Bovine Fresh Frozen Plasma Obtained for Transfusion by D. Proverbio, E. Spada, L. Baggiani, G. Bagnagatti De Giorgi, N. Roggero, A. Belloli, D. Pravettoni, R. Perego

“…This study has shown IgG protein fraction stability in bovine fresh frozen plasma collected for transfusion; therefore, bovine fresh frozen plasma seems to be suitable for the treatment of hypogammaglobulinemia (failure of passive transfer) in calves when stored for 12 months at −20°C.…”
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Osteocalcin Alleviates Nonalcoholic Fatty Liver Disease in Mice through GPRC6A by Mingliang Zhang, Xiaomin Nie, Yeqing Yuan, Yansu Wang, Xiaojing Ma, Jun Yin, Yuqian Bao

“…However, the potential mechanisms remain unclear. Although the G protein-coupled receptor family C group 6 subtype A (GPRC6A) is the putative receptor of osteocalcin, there is no direct evidence showing that GPRC6A mediates the effects of uncarboxylated osteocalcin in alleviating NAFLD in mice. …”
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Integration of ATAC-seq and RNA-seq reveals signal regulation during post-molt and inter-molt stages in muscle of Eriocheir sinensis by Bin Wang, Bin Wang, Jingjing Li, Mingzhi Zhang, Mingzhi Zhang, Anqi Li, Zhentao Song, Zhentao Song, Zhen He, Zhen He, Ruiqi Zhang, Ruiqi Zhang, Tong Hao, Tong Hao, Jinsheng Sun, Jinsheng Sun

“…GO enrichment analysis revealed that the up-regulated genes are largely associated with protein phosphorylation and phosphorus metabolism, while the down-regulated genes are mainly involved in DNA metabolism, transcription, cell adhesion, and G protein-coupled receptor (GPCR) signaling pathway. …”
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Integrative bioinformatic approach reveals novel melatonin-related biomarkers for Alzheimer’s disease by Hua-xiong Zhang, Dilmurat Hamit, Qing Li, Xiao Hu, San-feng Li, Fu Xu, Ming-yuan Wang, Guo-qing Bao, Hong-yan Li

“…After overlapping with 3,490 DEGs and 550 MRGs in GSE5281, four genes were found to be causally related to AD, namely, G protein-coupled receptor, family C, group 5, member B (GPRC5B), Methyltransferase-like protein 7 A (METTL7A), NF-κB inhibitor alpha (NFKBIA) and RAS association domain family 4(RASSF4). …”
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Progesterone induces meiosis through two obligate co-receptors with PLA2 activity by Nancy Nader, Lama Assaf, Lubna Zarif, Anna Halama, Sharan Yadav, Maya Dib, Nabeel Attarwala, Qiuying Chen, Karsten Suhre, Steven Gross, Khaled Machaca

“…This PLA2 activity bifurcates P4 signaling by inducing clathrin-dependent endocytosis of mPRβ, resulting in the production of lipid messengers that are G-protein coupled receptor agonists. Therefore, P4 drives meiosis by inducing an ABHD2 PLA2 activity that requires both mPRβ and ABHD2 as obligate co-receptors.…”
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