In vitro antiplasmodial activity of six plants against chloroquine-sensitive and resistant strains of Plasmodium falciparum by Samuel Korsah

“…Method: Aqueous extracts were prepared from the plants by decoction and freeze-dried. A fluorescence-based SYBR Green assay was used to evaluate the antimalarial activity of the extracts against Plasmodium falciparum strains 3D7 and DD2. …”
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Experimental Operation of a Prototype 750C Advanced Chloride Molten Salt Bellows Valve by Kenneth Armijo, Aaron Overacker, Ansel Blumenthal, Jeffrey Parish, Michael Nelson, Ivica Radman, Javier Martell

“…This design includes an integrated closed-loop thermal control system to ensure robust design for freeze-thaw cycles. The self-contained thermal management STM system, is unique in the salt valve industry since it is an integrated solution to provide a consistent, repeatable alternative to typical heat tracing. …”
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Synthesis and characterization of a magnetic bacterial cellulose-chitosan nanocomposite and evaluation of its applicability for osteogenesis by Nahid Rezazadeh, Effat Alizadeh, Somaieh Soltani, Soodabeh Davaran, Neda Esfandiari

“…Methods: The magnetic nanocomposite was prepared by immersing Fe3O4 in a mixture of bacterial cellulose-chitosan scaffold and then freeze-drying it. The resulting nanocomposite was characterized using FE-SEM and FTIR techniques. …”
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Iron-oxidizing microorganisms affect the iron-bound organic carbon in the subsoil of alpine grassland during the thawing of seasonal frozen soil by Yuxin Tian, Maidinuer Abulaizi, Zailei Yang, Tianle Kou, Yuanbin Jia, Yunpeng Hu, Mo Chen, Mo Chen, Mo Chen, Hongtao Jia, Hongtao Jia, Hongtao Jia

“…Soil moisture (SM) and FeOB were significant indirect factors affecting Fe-OC changes. Freeze–thaw changes in the subsoil of alpine grassland in Central Asia significantly affected FeOB and Fe oxides, thus affecting the Fe-OC content. …”
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Quantification of Myocardial Biomarkers in Sudden Cardiac Deaths Using a Rapid Immunofluorescence Method for Simultaneous Biomarker Analysis by Matteo Antonio Sacco, Valerio Riccardo Aquila, Saverio Gualtieri, Roberto Raffaele, Maria Cristina Verrina, Lucia Tarda, Santo Gratteri, Isabella Aquila

“…Contrary to prior concerns, PMI showed no significant correlation with marker levels in samples handled without freeze–thaw cycles. Issues related to hemolysis were addressed, and no significant effects were observed from resuscitation maneuvers. …”
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Effect of Bovine Serum Albumin (BSA) Concentration on Cryopreservation of Booroolong Frog Sperm with Evaluation of Post-Thaw Motility in Caffeine by Zara M. Anastas, Aimee J. Silla, Phillip G. Byrne, Rebecca J. Hobbs, Michael S. McFadden, Jonathan Daly, Justine K. O’Brien

“…The aim of this study was twofold: (1) to investigate the effect of the addition of bovine serum albumin (BSA) to the cryopreservation medium (pre-freeze), and (2) the effect of the addition of caffeine to the activation medium (post-thaw), on post-thaw sperm characteristics in the critically endangered Booroolong frog (<i>Litoria booroolongensis</i>). …”
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The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine by Shukui Zhou, Ying Wang, Kaile Zhang, Nailong Cao, Ranxing Yang, Jianwen Huang, Weixin Zhao, Mahbubur Rahman, Hong Liao, Qiang Fu

“…The ADSC sheets were fabricated and subsequently decellularized using repeated freeze-thaw, Triton X-100 and SDS decellularization. …”
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Influence of Drying Methods on the Morphological Features, Microstructural Properties, and Antioxidant Performance of <i>Floccularia luteovirens</i>: A Metabolomic Analysis by Mengjun Xiao, Tao Wang, Chuyu Tang, Min He, Xiaojian Pu, Tingjing Zhao, Yuling Li

“…This research employed an untargeted metabolomics (LC-MS/MS) strategy to elucidate the similarities and differences in the morphological characteristics, microstructure, antioxidant capacity, and metabolic profiles of <i>F. luteovirens</i> subjected to three distinct drying methods: natural air-drying (YG), oven-drying (HG), and vacuum freeze-drying (DG). Our findings indicated that the color of <i>F. luteovirens</i> samples dried using the YG and HG methods was yellow-brown, exhibiting a high degree of browning, whereas the samples processed by the DG method displayed a golden-yellow hue and a desirable fullness. …”
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Efficacy Study of Carrageenan as an Alternative Infused Material (Filler) in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Porous 3D Scaffold by Nor Syamimi Che Johari, Syazwan Aizad, Saiful Irwan Zubairi

“…Carrageenan was later infused into 3D porous scaffolds under vacuum pressure and freeze-drying process. Five carrageenan concentrations were prepared and its physicochemical properties such as pH and viscosity were carried out on each concentration to determine the best solutions to produce a new composite 3D structure. …”
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Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats by Katarzyna Papierska, Ewa Ignatowicz, Jadwiga Jodynis-Liebert, Małgorzata Kujawska, Róża Biegańska-Marecik

“…The aim of this study was to investigate the potential adverse effects and antioxidant properties of freeze-dried kale leaves in 90-day dietary experiment on Wistar rats. …”
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Anti-arthritic potential of crude sulfated polysaccharide from marine macroalgae Sargassum ilicifolium (Turner) C. Agardh: Regulation of cytokine cascade by Ramamoorthi Lavanya, Jeyabalan Srikanth, Sankar Seethalakshmi, Begum M. Yasmin, Duraipandian Chamundeeswari, Sekar Mahendran, Wong Ling Shing, Subramaniyan Vetriselvan

“…In the current work, we extracted the sulfated polysaccharide from a marine source and investigated its anti-arthritic potential in vivo. The isolated and freeze-dried polysaccharide was tested for acute oral toxicity based on OECD 423. …”
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Tr ansatlantic Trade and Investment Partnership: Consigned to Oblivion or Waiting for a Second Chance? by A. О. Mamedova

“…A question inevitably arises: what can account for the freeze of negotiations? Was it the result of Donald Trump’s election, who was a vocal critic of his predecessor’s legacy during his election campaign? …”
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Early Effects of Bronchoscopic Cryotherapy in Metastatic Non-Small Cell Lung Cancer Patients Receiving Immunotherapy: A Single-Center Prospective Study by Gediminas Vasiliauskas, Evelina Žemaitė, Erika Skrodenienė, Lina Poškienė, Gertrūda Maziliauskienė, Aurimas Mačionis, Skaidrius Miliauskas, Donatas Vajauskas, Marius Žemaitis

“…<b>Background/Objectives:</b> Cryotherapy is used for local tissue destruction through rapid freeze–thaw cycles. It induces cancer cell necrosis followed by inflammation in the treated tumor microenvironment, and it stimulates systemic adaptive immunity. …”
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Whey Protein Hydrogels and Emulsion Gels with Anthocyanins and/or Goji Oil: Formation, Characterization and In Vitro Digestion Behavior by Abdullah S. Seddiek, Kaiwen Chen, Fanlin Zhou, Muhindo Mwizerwa Esther, Abdelaziz Elbarbary, Hazem Golshany, Angelo Uriho, Li Liang

“…The co-inclusion of blueberry extract and goji oil increased the syneresis during the freeze–thaw cycles, with the values rising from 13% to 36% for 5% BE hydrogel and BE-containing emulsion gels after the first cycle. …”
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Effect of Arthrospira platensis (Spirulina) as a dietary supplement during transition period in dairy buffaloes by Giuseppe De Rosa, Vittoria L. Barile, Carmela M.A. Barone, Claudia Lambiase, Francesco Serrapica, Giovanna De Matteis, Emanuela Rossi, Corrado Pacelli

“…In top dressing mode, the S group additionally received 50 g/head/d of freeze-dried Spirulina for one month before calving and 100 g/head/d for 90 d after calving. …”
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Biointegration of soft tissue-inspired hydrogels on the chorioallantoic membrane: An experimental characterization by Manuel P. Kainz, Mathias Polz, Daniel Ziesel, Marta Nowakowska, Muammer Üçal, Sabine Kienesberger, Sophie Hasiba-Pappas, Raimund Winter, Nassim Ghaffari Tabrizi-Wizsy, Sarah Kager, Theresa Rienmüller, Julia Fuchs, Michele Terzano, Christian Baumgartner, Gerhard A. Holzapfel

“…Composite hydrogels of polyvinyl alcohol and Phytagel were fabricated by sterile freeze-thawing. CAM assays, as an alternative to traditional in vivo models, enabled the evaluation of the compatibility, attachment, and biointegration of hydrogels with three distinct compositions. …”
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