Mesenchymal stem cell-derived exosome and liposome hybrids as transfection nanocarriers of Cas9-GFP plasmid to HEK293T cells. by Behnaz Gharehchelou, Mehrnoush Mehrarya, Yahya Sefidbakht, Vuk Uskoković, Fatemeh Suri, Sareh Arjmand, Farnaz Maghami, Seyed Omid Ranaei Siadat, Saeed Karima, Massoud Vosough

“…These exosomes were then hybridized with liposomes using freeze-thaw cycles and direct mixing techniques to evaluate whether this combination enhances the transfection efficiency of large plasmids. …”
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Cryo-SEM Investigation of Chlorella Using Filter Paper as Substrate by Peng Wan, Meiyue Tao, Yumeng Zhou, Wenjun Han, Jianxia Wang, Jinghan Wang

“…By employing filter paper as the sample substrate, we propose a simple and reliable method for cryo-fixation and freeze-fracture of Chlorella sorokiniana in water suspension. …”
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Volatile Organic Compounds, Total Phenolic Content, Color, and Heating Uniformity of Lemon Peel as Affected by Rotational Speed of Turntable During Microwave Drying by Sevilay San, Işıl Barutçu Mazı

“…The quality analyses were also performed in freeze-dried peels. During microwave drying with rotation, speed of turntable affected the surface temperature values and TPC depending on the power level but did not exert a clear effect on the homogeneity of the surface temperature distribution and the color parameters. …”
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Fetal Spinal Cord Tissue in Mini-Guidance Channels Promotes Longitudinal Axonal Growth after Grafting into Hemisected Adult Rat Spinal Cords by Norman I. Bamber, Huaying Li, Patrick Aebischer, Xiao Ming Xu

“…In control rats, the FSC-channel congraft struct was exposed to 5 freeze/thaw cycles to produce non-viable grafts before implantation into the hemisected cord. …”
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Mechanical and Durable Performance of Lime-Steel Slag-Coal Gangue Mixtures Prepared by a Uniform Design Method for Pavement Base Applications by Hanlong CUI, Bingyang DENG, Chao WANG, Shenglei FENG, Yapeng ZHANG, Wenqing MENG, Xin LIU, Hao JI

“…There is no obvious microscopical difference before and after freeze-thaw cycles. The immersion expansion rate of the mixtures was far less than 1.5 %.…”
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Performance Evaluation of Recycled Asphalt Mixtures Containing Construction and Demolition Waste Applicated as Pavement Base by Le Ding, Junhui Zhang, Bowen Feng, Cheng Li

“…Subsequently, the uniaxial compression test, splitting tensile test, immersion Marshall test, freeze-thaw splitting test, and Hamburg rutting test were performed to investigate mechanical properties, water stability, and high-temperature stability of RAM. …”
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Cricket Flour and Pullulan Microparticle Formation via Electro-Blow Spinning as a New Method for the Protection of Antioxidant Compounds from Fruit Extracts by Sabina I. Wilkanowicz, Keara T. Saud

“…Electro-blow spinning (EBS) was used as a high throughput technique for encapsulation and compared to freeze-drying. The particles’ morphology was analyzed via scanning electron microscopy (SEM). …”
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Effects of Different Concentrations of Eugenol on the Structure and Emulsion Gel Properties of Myofibrillar Protein by Jiaqi LI, Deyin PAN, Jinming MA, Jingjing DIAO, Hongsheng CHEN

“…In contrast, upon the addition of high-concentration eugenol (100 mg/g), the gel strength and water retention were significantly decreased, the cooking loss and freeze-thaw loss were significantly increased, and the gel network structure became rough, and the pore size increased, as revealed by the scanning electron microscopy images. …”
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Experimental study of polyurethane-cement composite reinforcing talik beneath subgrade in permafrost regions: Exothermic characteristics, durability and micro-mechanism by Fujun Niu, Yuru Wang, Ziyi Wang, Zekun Ding, Minghao Liu

“…The results show that the talik solidified by WPU10 has good water stability and relatively low strength damage during the freeze-thaw cycles. Freeze-thaw cycles affect the pore structure, the morphology of hydration products within the specimens, and the effective porosities of PUC and WPU10 increase by 62.44% and 59.58%, respectively. …”
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Trends in Florida Citrus with Emphasis on Indian River, St. Lucie, Martin, and Palm Beach Counties by Jackie K. White, P.J. van Blokland

“… FE636, a 9-page fact sheet by Jackie K White and P.J. van Blokland, examines how hurricanes, freezes, citrus diseases and pests, competition with Brazil, and a booming population growth are affecting the citrus industry of Florida's Treasure Coast. …”
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Trends in Florida Citrus with Emphasis on Indian River, St. Lucie, Martin, and Palm Beach Counties by Jackie K. White, P.J. van Blokland

“… FE636, a 9-page fact sheet by Jackie K White and P.J. van Blokland, examines how hurricanes, freezes, citrus diseases and pests, competition with Brazil, and a booming population growth are affecting the citrus industry of Florida's Treasure Coast. …”
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Fruit By-Product Processing and Bioactive Compounds by Mohammed Saleh, Lana Amro, Haya Barakat, Rahma Baker, Amani Abu Reyash, Reham Amro, Jihad Qasem

“…Total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant potential (FRAP), and diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of avocado peel, avocado seeds, kiwi fruit, orange peel, pineapple peel, and pomegranate skin by-products during processing (i.e., dried, blanched, freezed, and fresh) were determined. It was hypothesized that fruit by-products would add a significant value to the food products. …”
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Turmeric-black pepper-honey nanoemulsion formulation and antiulcerogenic effect evaluation against ethanol-induced gastric ulcers in rats. by Amirah Adlia, Cynthiana Christabella Aslan, Lia Safitri, I Ketut Adnyana

“…Two stability evaluations were carried out, i.e., storage and freeze-thaw stability tests. The organoleptic, droplet size, polydispersity index, pH, viscosity, and curcumin content of the nanoemulsion were evaluated. …”
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Spent coffee grounds as a sustainable coffee flavouring ingredient in muffins by Sina Breian Solberg, Svein Øivind Solberg

“…The vacuum dried was the grainiest and the freeze dried had the least coffee flavour. Ranking data showed the control in top and the vacuum dried bottom. …”
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Evaluation of the performance of diagnostic methods of canine parvovirus-2 and canine enteric coronavirus infections under different storage conditions and determination of molecul... by Irmak Dik, H. Pelin Aslim, Oya Bulut

“…Besides, it was aimed to determine the sensitivity and specificity rates of the diagnostic tests after fresh or freeze-thawed stool for infection diagnosis.<p> <b>Materials and Methods: </b>Faecal samples were collected from 50 unvaccinated, 0-12 months old dogs with diarrhoea symptoms at the shelter. …”
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Method of obtaining and storing hyperimmune anthrax serum by S. V. Ivanova, L. A. Melnikova, A. P. Rodionov, V. V. Evstifeev

“…For long-term storage of the serum produced, the freeze-drying modes were optimized, giving 2% residual moisture content of the finished product. …”
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Preparation and transfer of Burkholderia mallei production strain 5584 in accordance with the biosafety requirements by E. A. Artemeva, L. A. Melnikova, A. P. Rodionov

“…As the work progressed, Burkholderia mallei strain 5584 culture was isolated and freeze-dried. Before the transfer, biological properties of the freeze-dried Burkholderia mallei strain 5584 were studied for their compliance with the passport data. …”
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Experimental Results on Charge Fluctuations in Heavy-Ion Collisions by D. K. Mishra, P. Garg, P. K. Netrakanti, L. M. Pant, A. K. Mohanty

“…We also discuss the extraction of the freeze-out parameters using particle ratios and experimentally measured higher moments of net-charge fluctuations. …”
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Conventional and Specialty Eggplant Varieties in Florida by Monica Ozores-Hampton

“…In south Florida, where winters are mild and freezes are infrequent, eggplants are planted from August to March. …”
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Conventional and Specialty Eggplant Varieties in Florida by Monica Ozores-Hampton

“…In south Florida, where winters are mild and freezes are infrequent, eggplants are planted from August to March. …”
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