pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity by Sofia Fernanda Gonçalves Zorzella-Pezavento, Fernanda Chiuso-Minicucci, Thais Graziela Donegá França, Larissa Lumi Watanabe Ishikawa, Larissa Camargo da Rosa, Priscila Maria Colavite, Bianca Balbino, Camila Marques, Maura Rosane Valerio Ikoma, Ana Paula Masson, Célio Lopes Silva, Alexandrina Sartori

“…MOG35–55 immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. …”
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Synergistic Synbiotic-Containing <i>Lactiplantibacillus plantarum</i> and Fructo-Oligosaccharide Alleviate the Allergenicity of Mice Induced by Soy Protein by Jing Bai, Qian Zeng, Wen Den, Liheng Huang, Zhihua Wu, Xin Li, Ping Tong, Hongbing Chen, Anshu Yang

“…Furthermore, Lp–FOS intervention upregulated the mRNA levels of T-bet and Foxp3 and downregulated the mRNA levels of GATA3. …”
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Clinical Significance of Peripheral Blood T Lymphocyte Subsets in Helicobacter pylori-Infected Patients by Yuka Satoh, Hatsue Ogawara, Osamu Kawamura, Motoyasu Kusano, Hirokazu Murakami

“…In order to clarify the immunological changes induced by this infection, we determined the percentage and, as indicated, ratios of the following cells in peripheral blood of 45 H. pylori-infected patients and 21 control subjects: CD4+ T cell, CD8+ T cells, T helper 1 cells (Th1), T helper 2 cells (Th2), CD4+CD25+ T cells, Foxp3+ regulatory T cells (Tregs), CD4/CD8 ratio, and Th1/Th2 ratio. …”
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The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System by Sara Trabanelli, Federico La Manna, Marco Romano, Valentina Salvestrini, Michele Cavo, Marilena Ciciarello, Roberto M. Lemoli, Antonio Curti

“…Specifically, the weak downregulation of CD80 and CD86 caused by Det-DOC on DC surface results in a weak modulation of DC functions, which indeed retain a high capacity to induce T-cell proliferation and to generate CD4+CD25+Foxp3+ T cells. Moreover, Det-DOC enhance the DC capacity to differentiate CD4+CD161+CD196+ Th17-cells by upregulating IL-6 secretion. …”
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Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis by Mónica Kurte, Javiera Bravo-Alegría, Alexander Torres, Vania Carrasco, Cristina Ibáñez, Ana María Vega-Letter, Catalina Fernández-O’Ryan, Carlos E. Irarrázabal, Fernando E. Figueroa, Rodrigo A. Fuentealba, Claudia Riedel, Flavio Carrión

“…Interestingly, regardless of time administration, MSCs significantly reduced IL-6 and also T-bet, RORγT, and Foxp3 mRNA levels in brain samples of EAE mice. The downregulation of IL-6 could restore the well-functioning of the blood-brain barrier of EAE mice, correlated with a decreased number of brain infiltrating leukocytes. …”
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DC-Based Immunotherapy Combined with Low-Dose Methotrexate Effective in the Treatment of Advanced CIA in Mice by Jun-Eui Park, Jinah Jang, Ji-Hye Choi, Mi-sun Kang, Yun-Ju Woo, Young-Rim Seong, Chan-Bum Choi, Hye-Soon Lee, Sang-Cheol Bae, Yong-Soo Bae

“…The effect of CII-smDCs alone was also comparable to the combination therapy. CD4+Foxp3+ Treg populations and IL-10 secretion markedly increased, and CII-specific autoreactive T cells decreased in mice treated with CII-smDCs alone or in combination with MTX. …”
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Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi by Dania López-Moreno, Zeinab Yazdi, Francisco N. Morales-Serna, Juan M. Martínez-Brown, Leonardo Ibarra-Castro, Alejandra García-Gasca, Selene M. Abad-Rosales, Rodolfo Lozano-Olvera, Emma J. Fajer-Ávila, Esteban Soto

“…RT-qPCR assays were developed to investigate the expression of il1B, il8, il10, il12, il17, igM, igT, ifnγ, tnfα, tbet, hsp70, foxp3a, stat4, and cmip in gills and head kidney. …”
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Computational network analysis of two popular skin cancers provides insights into the molecular mechanisms and reveals common therapeutic targets by Md Sujan Mahmud, Bikash Kumar Paul, Md. Rakibul Hasan, K.M. Tanjida Islam, Imran Mahmud, Shahin Mahmud

“…Our analysis revealed 22 common genes between BCC and AK: TP53, EGFR, CDKN2A, MMP9, PTGS2, VDR, BCL2, MMP2, EZH2, TP63, FOXP3, MSH2, MMP14, FLG, MC1R, CDKN2B, TIMP3, TYR, SOX10, IRF4, KRT17, and NID1. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…The levels of IL-33, orphan nuclear receptor gamma t (RORγt), and forkhead transcription factor protein 3 (FOXP3) protein in lung tissue were evaluated by Western blotting. …”
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Therapeutic Efficacy of <i>Lavandula dentata</i>’s Oil and Ethanol Extract in Regulation of the Neuroinflammation, Histopathological Alterations, Oxidative Stress, and Restoring Ba... by Aziza Antar, Eman S. Abdel-Rehiem, Areej A. Al-Khalaf, Abdelaziz S. A. Abuelsaad, Mohamed Abdel-Gabbar, Gaber M. G. Shehab, Ayman M. Abdel-Aziz

“…Moreover, <i>L. dentata’s</i> oil and ethanol extract re-balances T-reg/Th-17 cytokines, restoring the pro/anti-inflammatory cytokines and Treg markers, e.g., FOXP3 and CTLA-4, to their normal level. <b>Conclusions</b>: The present work confirms that the extract and essential oils of <i>L. dentata</i> L. have different activities to ameliorate the progression of histopathological alterations. …”
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Sequence-structure based prediction of pathogenicity for amino acid substitutions in proteins associated with primary immunodeficiencies by Ekaterina S. Porfireva, Anton D. Zadorozhny, Anastasia V. Rudik, Dmitry A. Filimonov, Alexey A. Lagunin, Alexey A. Lagunin

“…In this study, we have developed classification sequence-structure-property relationships (SSPR) models for predicting the pathogenicity of amino acid substitutions (AAS) in 25 proteins associated with the most important and genetically studied PIDs and encoded genes: IL2RG, JAK3, RAG1, RAG2, ADA, DCLRE1C, CD40LG, WAS, ATM, STAT3, KMT2D, BTK, FOXP3, AIRE, FAS, ELANE, ITGB2, CYBB, G6PD, GATA2, STAT1, IFIH1, NLRP3, MEFV, and SERPING1.MethodsThe data on 4825 pathogenic and benign AASs in the selected proteins were extracted from ClinVar and gnomAD. …”
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Adoptive Cell Therapy with Tumor-Infiltrating Lymphocytes in Advanced Melanoma Patients by Mélanie Saint-Jean, Anne-Chantal Knol, Christelle Volteau, Gaëlle Quéreux, Lucie Peuvrel, Anabelle Brocard, Marie-Christine Pandolfino, Soraya Saiagh, Jean-Michel Nguyen, Christophe Bedane, Nicole Basset-Seguin, Amir Khammari, Brigitte Dréno

“…Notably, patients treated with high percentages of CD4 + CD25 + CD127lowFoxp3+ T cells among their TILs had significantly shorter OS. …”
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Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study by Janette Furuzawa-Carballeda, Guadalupe Lima, Perla Simancas, Dolores Ramos-Bello, Margaret Simancas, Ian C. Bostock, Mario Vilatobá, Bernardo Gabilondo, Julio Granados, Luis Morales-Buenrostro, Josefina Alberú, Luis Llorente

“…Regulatory Foxp3-expressing T cells (Tregs), IL-10-producing B cells (Bregs), and IDO-expressing dendritic cells (DCregs) downregulate inflammatory processes and induces peripheral tolerance. …”
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Correlation and diagnostic significance of CD4 T cell subsets and NLRP3 inflammasome in ulcerative colitis: the role of the NLRP3/T-bet/GATA3 axis by Yingnan Hu, Jingyi Tang, Dian Yu, Shuo Su, Jintao Fang, Linying Xia, Wenjun Xu, Weihan Zhu, Ninping Song, Fengyong Wang, Dechang Diao, Wei Zhang

“…Additionally, Th1 and Th2 cytokines (IL-2 and IL-4) were significantly elevated in severe UC, while Treg markers (FOXP3, IL-10, TGF-β1) were elevated only in mild-to-moderate UC. …”
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Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation by Laura Adalid-Peralta, Asiel Arce-Sillas, Gladis Fragoso, Graciela Cárdenas, Marcos Rosetti, Didier Casanova-Hernández, Claudia Rangel-Escareño, Laura Uribe-Figueroa, Agnes Fleury, Edda Sciutto

“…Monocyte-derived dendritic cells (DC) from healthy human donors, cocultivated with autologous CD4+ naïve cells either in the presence or absence of cysticerci, promoted CD25highFoxp3+ Treg differentiation. An increased Treg induction was observed when cysticerci were present. …”
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Prognostic value of tumor‐infiltrating lymphocytes and PD‐L1 expression in esophageal squamous cell carcinoma by Jie Hu, Takeshi Toyozumi, Kentaro Murakami, Satoshi Endo, Yasunori Matsumoto, Ryota Otsuka, Tadashi Shiraishi, Shinichiro Iida, Hiroki Morishita, Tenshi Makiyama, Yuri Nishioka, Masaya Uesato, Koichi Hayano, Akira Nakano, Hisahiro Matsubara

“…Immunohistochemical analysis and multiplex immunofluorescence for CD4, CD8, CD25, FOXP3, and PD‐L1 in the tumor were used to identify multiple tumor‐infiltrating immune cells (TIIC), Tregs, and TC. …”
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Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients by Aline Michelle Barbosa Bafica, Luciana Santos Cardoso, Sérgio Costa Oliveira, Alex Loukas, Alfredo Góes, Ricardo Riccio Oliveira, Edgar M. Carvalho, Maria Ilma Araujo

“…These two antigens increased the expression of CTLA-4 in CD4+ T cells and they also expanded the frequency of CD4+CD25highFoxp3+ T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.…”
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The Role of Circulating Regulatory T Cell Levels on Subclinical Atherosclerosis and Cardiovascular Risk Factors in Women with Systemic Lupus Erythematosus by Mario García-Carrasco, Pamela Soto-Santillán, Claudia Mendoza-Pinto, Rebeca González-Ramírez, Ana Lidia López-Carmona, Pamela Munguía-Realpozo, Ivet Etchegaray-Morales, Socorro Méndez-Martínez, José Luis Gálvez-Romero, Aurelio López-Colombo, Alejandro Ruiz-Arguelles

“…Flow cytometry was used to analyze Foxp3 and Helios expression in peripheral blood CD4 T cells. …”
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Mechanistic investigation and the optimal dose based on baicalin in the treatment of ulcerative colitis–A preclinical systematic review and meta-analysis by Jinchen Chong, Zepeng Chen, Jiaze Ma, Linhai He, Yijia Zhu, Zhihua Lu, Zhengxi Qiu, Chen Chen, Yugen Chen, Feng Jiang

“…Baicalin significantly modulated various biomarkers: HCS (SMD = -3.91), DAI (MD = -2.75), spleen index (MD = -12.76), MDA (SMD = -3.88), IL-6 (SMD = -10.59), IL-1β (SMD = -3.98), TNF-α (SMD = -8.05), NF-κB (SMD = -5.46), TLR4 (MD = -0.38), RORγ (MD = -0.89), MCP-1 (MD = -153.25), MPO (SMD = -7.34), Caspase-9 (MD = -0.93), Caspase-3 (MD = -0.45), FasL (MD = -1.20)) and enhanced BWC (MD = 0.06), CL (MD = 1.39), ZO-1 (MD = 0.44), SOD (SMD = 3.04), IL-10 mRNA (MD = 3.14), and FOXP3 (MD = 0.45) levels. Baicalin’s actions may involve the PI3K/AKT, TLR4/NF-κB, IKK/IKB, Bcl-2/Bax, Th17/Treg, and TLRs/MyD88 pathways. …”
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CD4+CD25+调节性T细胞的体外扩增

“…培养第7、14、21天收集细胞计数，第21天收集细胞行流式细胞术三标法检测CD4、CD25、FOXP3的表达。MTT法检测体外扩增的CD4+CD25+Treg对自体和异体的CD4+T细胞增殖的抑制作用。 …”
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