Serum Pharmacochemistry and Network Pharmacology Reveal Active Compounds and Mechanisms of the Huaxian Formula in Alleviating Radiation-Induced Pulmonary Fibrosis by Gong C, Chen J, Zou P, Fang Z, Quan L, Wang J, Yin J, Lin B, Lang J, Chen M

“…The Huaxian formula (HXF), a traditional Chinese herbal remedy, shows promise in alleviating RIPF; however, its active ingredients and underlying mechanisms remain poorly understood.Methods: Through serum pharmacochemistry, network pharmacology, molecular docking, and experimental validation, we investigate the potential mechanisms of HXF in the prevention and treatment of radiation-induced pulmonary fibrosis (RIPF).Results: Histological examination and non-invasive computed tomography (CT) scans in animal experiments revealed that HXF improved extracellular matrix collagen deposition in the lung tissue of irradiated mice and reduced fibrosis manifestations on CT images. …”
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Deficiency of ATF3 facilitates both angiotensin II‐induced and spontaneously formed aortic aneurysm and dissection development by activating cGAS–STING pathway by Yifan Du, Poyi Hu, Xiangchao Ding, Dashuai Wang, Jingjing Luo, Sheng Le, Lingyun Ren, Manhua Chen, Ping Ye, Jiahong Xia

“…Abstract Background Sporadic aortic aneurysm and dissection (AAD) is a critical condition characterised by the progressive loss of vascular smooth muscle cells (VSMCs) and the breakdown of the extracellular matrix. However, the molecular mechanisms responsible for the phenotypic switch and loss of VSMCs in AAD are not fully understood. …”
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Biotechnological Phytocomplex of <i>Zanthoxylum piperitum</i> (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo by Giovanna Rigillo, Giovanna Pressi, Oriana Bertaiola, Chiara Guarnerio, Matilde Merlin, Roberto Zambonin, Stefano Pandolfo, Angela Golosio, Francesca Masin, Fabio Tascedda, Marco Biagi, Giulia Baini

“…This study proposed a novel biotechnological <i>Z. piperitum</i> phytocomplex (ZPP) obtained by plant cell culture for skin health, specifically targeting collagen synthesis, extracellular matrix stability, and resilience against cellular stress. …”
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Overexpression of MMP13 Is Associated with Clinical Outcomes and Poor Prognosis in Oral Squamous Cell Carcinoma by Vui King Vincent-Chong, Iman Salahshourifar, Lee Peng Karen-Ng, Ming Yhong Siow, Thomas George Kallarakkal, Anand Ramanathan, Yi-Hsin Yang, Goot Heah Khor, Zainal Ariff Abdul Rahman, Siti Mazlipah Ismail, Narayanan Prepageran, Wan Mahadzir Wan Mustafa, Mannil Thomas Abraham, Keng Kiong Tay, Sok Ching Cheong, Rosnah Binti Zain

“…Matrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis. …”
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Depot-specific acetylation profiles of adipose tissues—therapeutic targets for metabolically unhealthy obesity by Haoyue Guo, Zhiyi Zhang, Juntao Yang, Jiangfeng Liu, Hongwei Lin, Ningbei Yin

“…Upregulated proteins and acetylated proteins in SA were predominantly enriched in extracellular matrix (ECM) remodeling pathways, while those in VA were enriched in energy metabolism and disease-related pathways. …”
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Host–virus interactions during infection with a wild-type ILTV strain or a glycoprotein G deletion mutant ILTV vaccine strain in an ex vivo system by Gayathri Gopakumar, Mauricio J.C. Coppo, Andrés Diaz-Méndez, Carol A. Hartley, Joanne M. Devlin

“…Additionally, several other biological processes, molecular functions, and cellular components were enriched uniquely in the ∆gG-ILTV-infected TOCs, including those that indicated modifications to tracheal extracellular matrix (ECM) structural components, which may have a role in immune modulation in vivo. …”
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CD133+PD-L1+ cancer cells confer resistance to adoptively transferred engineered macrophage-based therapy in melanoma by Jiaojiao Xu, Zhe Li, Qinli Tong, Sihang Zhang, Jianchen Fang, Aihua Wu, Guoguang Wei, Chen Zhang, Sheng Yu, Binbin Zheng, Hongzheng Lin, Xueling Liao, Zeyu Xiao, Wei Lu

“…Compared to the CD133-PD-L1- cancer cells, the CD133+PD-L1+ cancer cells express higher transforming growth factor-β signaling molecules to foster a resistant tumor niche, that restricts the trafficking of the transferred macrophages by stiffened extracellular matrix, and inhibits their cell-killing capability by immunosuppressive factors. …”
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Integrated transcriptomic hypothalamic-pituitary-ovarian axis network analysis reveals the role of energy availability on egg production in layers by Jianling Peng, Yong Cui, Haiping Liang, Shenyijun Xu, Linjian Weng, Meng Ru, Ramlat Ali, Qing Wei, Jiming Ruan, Jianzhen Huang

“…Consequently, this suppression of the hypothalamus and pituitary affected ovarian function through ovarian steroidogenesis and the extracellular matrix (ECM)-receptor interaction. These findings suggest that energy deprivation inhibits the function of the HPO axis, leading to impaired follicle development and reduced egg production, and that refeeding can partially restore these indicators.…”
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Mesenchymal stromal cells-derived extracellular vesicles in cartilage regeneration: potential and limitations by María Piñeiro-Ramil, Iván Gómez-Seoane, Ana Isabel Rodríguez-Cendal, Isaac Fuentes-Boquete, Silvia Díaz-Prado

“…Most studies indicate that MSC-derived sEVs enhance chondrocyte proliferation, improve cartilage extracellular matrix composition, and reduce matrix-degrading enzymes and inflammation, thereby delaying OA progression. …”
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Cisplatin resistance alters ovarian cancer spheroid formation and impacts peritoneal invasion by Lydia C. Powell, Marcos Quintela, David W. James, Emenike Onyido, David Howard, Kadie Edwards, Jordan L. Turney, Charlotte R. Morgan, Jenny Worthington, Nicole Williams, Alexander Dulebo, Heiko Haschke, Deyarina Gonzalez, R. Steven Conlan, Lewis W. Francis

“…Gene expression arrays revealed upregulation of genes associated with cell adhesion, extracellular matrix (ECM) and epithelial-to-mesenchymal transition (EMT) in CR spheroids, while immunofluorescence assays demonstrated increased protein expression of PAI-1 (p &lt; 0.05; implicated in cell adhesion) and reduced protein expression of Tenascin-C (p &lt; 0.01; implicated in elasticity) in CR spheroids compared to P spheroids. …”
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β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis by Yijia Zhang, Xinya Zhu, Liyuan Chen, Tianyu Gao, Guang Chen, Jin Zhu, Guoyu Wang, Daiying Zuo

“…RNA-seq analysis of PDAC cells exposed to TAM-derived factors demonstrated enhanced mesenchymal transition and stemness properties, with direct enrichment of OSM signaling and extracellular matrix remodeling pathways. Mechanistically, β-catenin activation directly regulated both TAM phenotype and OSM expression, while TAM-conditioned medium enhanced PDAC cell migration, invasion, and lung metastasis. …”
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Umbilical Cord Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Modulate Skin Matrix Synthesis and Pigmentation by Kee LT, Foo JB, How CW, Nur Azurah AG, Chan HH, Mohd Yunus MH, Ng SN, Ng MH, Law JX

“…Therefore, this study aims to evaluate the impact of UC-MSC-sEVs on the synthesis of the skin’s extracellular matrix (ECM) and pigmentation using in vitro models.Methods: The study investigated the impact of characterized UC-MSC-sEVs on various aspects including the proliferation, migration, antioxidant activity, and ECM gene expression of human dermal fibroblasts (HDF). …”
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Antioxidant Properties of Tonsil-Derived Mesenchymal Stem Cells on Human Vocal Fold Fibroblast Exposed to Oxidative Stress by Sung-Chan Shin, Hyung-Sik Kim, Yoojin Seo, Cho Hee Kim, Ji Min Kim, Hyun-Keun Kwon, Jin-Choon Lee, Eui-Suk Sung, Byung-Joo Lee

“…However, few studies have examined the direct correlation between oxidative damage and reconstitution of extracellular matrix (ECM) in the vocal fold fibrosis. We, therefore, sought to investigate the impact of oxidative stress on cell survival and ECM production of human vocal fibroblasts (hVFFs) and the protective effects elicited by TMSCs against oxidative damages in hVFFs. hVFFs were exposed to different concentrations of tert-butyl hydroperoxide in the presence or absence of TMSCs. …”
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In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds by Michel Haagdorens, Vytautas Cėpla, Eline Melsbach, Laura Koivusalo, Heli Skottman, May Griffith, Ramūnas Valiokas, Nadia Zakaria, Isabel Pintelon, Marie-José Tassignon

“…Upon confluence, primary LESC showed high expression of the stem cell marker ΔNp63, proliferation marker cytokeratin (KRT) 14, adhesion markers integrin-β4 and E-cadherin, and LESC-specific extracellular matrix proteins laminin-α1, and collagen type IV. …”
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Paracrine activity of Smurf1-silenced mesenchymal stem cells enhances bone regeneration and reduces bone loss in postmenopausal osteoporosis by Alberto González-González, Itziar Álvarez-Iglesias, Daniel García-Sánchez, Monica Dotta, Ricardo Reyes, Ana Alfonso-Fernández, Alfonso Bolado-Carrancio, Patricia Díaz-Rodríguez, María Isabel Pérez-Núñez, José Carlos Rodríguez-Rey, Jesús Delgado-Calle, Flor M. Pérez-Campo

“…Proteomic analysis revealed both soluble and vesicular fractions of hCM-Smurf1 were enriched with proteins essential for ossification and extracellular matrix organization, enhancing osteogenic differentiation. …”
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Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo by Xiangwei Liu, Naiwen Tan, Yuchao Zhou, Xueying Zhou, Hui Chen, Hongbo Wei, Ji Chen, Xiaoru Xu, Sijia Zhang, Guodong Yang, Yingliang Song

“…The analyses in vitro showed that the overexpression of Sema3A in ASCs significantly enhanced the expression of bone-related genes and extracellular matrix calcium deposition, while decreasing the expression of adipose-related genes and thus lipid droplet formation, resembling a BMSCs phenotype. …”
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Herbal HuoXueTongFu Formula with anti-inflammatory and fibrinolytic activity regulation for the prevention of postoperative peritoneal adhesions by Lili Yang, Lili Yang, Yali Wang, Yali Wang, Zhengjun Li, Zhengjun Li, Wen Li, Yanqi Chen, Ziyang Kong, Huixiang Zhang, Jiafei Wu, Mingqi Shang, Ming Li, Yaoyao Bian, Yaoyao Bian, Yaoyao Bian, Li Zeng, Li Zeng

“…., matrix metalloproteinase 9 (MMP9), tissue-type plasminogen activator (tPA), and plasminogen activator inhibitor 1 (PAI-1), were annotated as important biological processes (extracellular matrix disassembly and the collagen catabolic process). …”
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Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis by Junxian Hu, Junxian Hu, Yibo Zhu, Yibo Zhu, Zeyu Pang, Zeyu Pang, Xiaoxiao Li, Xiaoxiao Li, Huilin Zhang, Huilin Zhang, Xiangwei Li, Xiangwei Li, Yongjian Gao, Yongjian Gao, Yiyang Wang, Yiyang Wang, Pei Li, Pei Li, Qiang Zhou, Qiang Zhou

“…These changes in extracellular matrix (ECM) composition helped maintain the physiological function of NP cells. …”
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Achalasia—An Autoimmune Inflammatory Disease: A Cross-Sectional Study by J. Furuzawa-Carballeda, D. Aguilar-León, A. Gamboa-Domínguez, M. A. Valdovinos, C. Nuñez-Álvarez, L. A. Martín-del-Campo, A. B. Enríquez, E. Coss-Adame, A. E. Svarch, A. Flores-Nájera, A. Villa-Baños, J. C. Ceballos, G. Torres-Villalobos

“…Achalasia tissue exhibited an increase in the expression of proteins involved in extracellular matrix turnover, apoptosis, proinflammatory and profibrogenic cytokines, and Tregs and Bregs versus controls (P<0.001). …”
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Oral submucous fibrosis: pathogenesis and therapeutic approaches by Jianfei Tang, Junjie Liu, Zekun Zhou, Xinyan Cui, Hua Tu, Jia Jia, Baike Chen, Xiaohan Dai, Ousheng Liu

“…Abstract Oral submucous fibrosis (OSF), characterized by excessive deposition of extracellular matrix (ECM) that causes oral mucosal tissue sclerosis, and even cancer transformation, is a chronic, progressive fibrosis disease. …”
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