The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis by Louise Tzung-Harn Hsieh, Belinda S Hall, Jane Newcombe, Tom A Mendum, Sonia Santana Varela, Yagnesh Umrania, Michael J Deery, Wei Q Shi, Josué Diaz-Delgado, Francisco J Salguero, Rachel E Simmonds

“…Hence supplementing mycolactone-depleted extracellular matrix may be a future therapeutic avenue, to improve wound healing rates.…”
Get full text

Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats by Xiaoru Xu, Kaixiu Fang, Lifeng Wang, Xiangwei Liu, Yuchao Zhou, Yingliang Song

“…The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. …”
Get full text

Predicting the Most Deleterious Missense Nonsynonymous Single-Nucleotide Polymorphisms of Hennekam Syndrome-Causing CCBE1 Gene, In Silico Analysis by Khyber Shinwari, Liu Guojun, Svetlana S. Deryabina, Mikhail A. Bolkov, Irina A. Tuzankina, Valery A. Chereshnev

“…Using state-of-the-art in silico tools, this study examined the most pathogenic nonsynonymous single-nucleotide polymorphisms (nsSNPs) that disrupt the CCBE1 protein and extracellular matrix remodeling and migration. Our results indicate that seven nsSNPs, rs115982879, rs149792489, rs374941368, rs121908254, rs149531418, rs121908251, and rs372499913, are deleterious in the CCBE1 gene, four (G330E, C102S, C174R, and G107D) of which are the highly deleterious, two of them (G330E and G107D) have never been seen reported in the context of Hennekam syndrome. …”
Get full text

Investigating the antioxidant and anti-inflammatory potential of Nypa fruticans: a multifaceted approach to skin protection and aging by Seul Ki Kim, Sang-Back Kim, Seul Bi Lee, Kang sub Kim, So-Ri Son, En Jin Choi, Byung Chul Park, Eunyoung Hong, You Ah Kim, Byoung Seok Moon, Sullim Lee

“…Abstract Reactive oxygen species (ROS) produced in the mitochondria of skin cells play a significant role in the degradation of the extracellular matrix and induction of inflammatory responses, both of which are major contributors to skin aging. …”
Get full text

In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells by Annunziata Mauro, Hashimita Sanyal, Angelo Canciello, Paolo Berardinelli, Valentina Russo, Nicola Bernabò, Luca Valbonetti, Barbara Barboni

“…Conversely, P4 exposure inhibited oAEC chondrogenic differentiation, which was induced in E2-treated cells as confirmed by the upregulation of chondrogenesis-related genes (SOX9, ACAN, and COL2A1) and by the accumulation of Alcian blue-positive extracellular matrix. Simultaneously, E2-treated cells remained unresponsive to osteogenic inductive stimuli. …”
Get full text

Anti-Scar Effects of Micropatterned Hydrogel after Glaucoma Drainage Device Implantation by Yiling Han, Qiangwang Geng, Aimeng Dong, Menglu Jiang, Jingyi Ma, Wulian Song, Pan Fan, Yuanyuan Li, Jiawen Gao, Fenghua Zhang, Jinsong Leng, Huiping Yuan

“…Moreover, the modified GDD effectively controlled intraocular pressure (IOP), mitigated fibrous formation, and remodeled extracellular matrix (ECM) collagen distribution in vivo. …”
Get full text

Undernutrition disrupts jejunal and ileal microbiota and epithelial tissue homeostasis in a pregnant sheep model by Weibin Wu, Muhammad Faheem Akhtar, Jiahong Geng, Huizhen Lu, Muhammad Ajwad Rahim, Jianbo Cheng, Xiaoling Ding, Shengyong Mao, Yanfeng Xue

“…In the jejunal epithelium, genes associated with extracellular matrix–receptor interactions, cell growth, and immune response were down-regulated (P < 0.05) upon undernutrition. …”
Get full text

TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton by Mona Elsafadi, Muthurangan Manikandan, Sami Almalki, Mohammad Mobarak, Muhammad Atteya, Zafar Iqbal, Jamil Amjad Hashmi, Sameerah Shaheen, Nehad Alajez, Musaad Alfayez, Moustapha Kassem, Raed Abu Dawud, Amer Mahmood

“…Bioinformatics analysis revealed that TGFβl treatment was associated with an enrichment of genes in the skeletal and extracellular matrix categories and the regulation of the actin cytoskeleton. …”
Get full text

Harnessing natural polymers and nanoparticles: Synergistic scaffold design for improved wound healing by Anitha S, Nandini Robin Nadar, Srividya Shivakumar, Sharma S C, Siddharth P, Surekha Varalakshmi V, Rajesh Lenka, Rajadurai S

“…The synergistic approach results in novel scaffold designs that promote cell functions and mimic the extracellular matrix (ECM) to further enhance wound healing. …”
Get full text

Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation by Julie Anne Côté, Frédéric Guénard, Julie Lessard, Marc Lapointe, Simon Biron, Marie-Claude Vohl, André Tchernof

“…Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1). …”
Get full text

Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF by Chary Marquez Batista, Leonardo Luis Torres Bianqui, Bruno Bonganha Zanon, Mauricio Menezes Aben Athar Ivo, Gabriela Pintar de Oliveira, Jessica Ruivo Maximino, Gerson Chadi

“…PEDF treatment to SC injury triggered neuroplasticity in lumbar CPG and regulation of neurotrophic factors, extracellular matrix molecules, and ephrins.…”
Get full text

Current Treatment Regimens and Promising Molecular Therapies for Chronic Hepatobiliary Diseases by Marilena Durazzo, Arianna Ferro, Victor Manuel Navarro-Tableros, Andrea Gaido, Paolo Fornengo, Fiorella Altruda, Renato Romagnoli, Søren K. Moestrup, Pier Luigi Calvo, Sharmila Fagoonee

“…Drugs targeting molecular pathways involved in chronic hepatobiliary diseases, such as inflammation, hepatic stellate cell activation and proliferation, and extracellular matrix production, are being developed. Etiology-specific treatments, such as those for hepatitis B and C viruses, are already in clinical use, and efforts to develop new, targeted therapies for other chronic hepatobiliary diseases are ongoing. …”
Get full text

Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…Serial evaluation of the expression of mesenchymal and extracellular matrix (ECM) markers revealed that HIOs have an adequately developed mesenchymal component, which gradually declines through culture passages. …”
Get full text

The GLP-1R agonist semaglutide reshapes pancreatic cancer associated fibroblasts reducing collagen proline hydroxylation and favoring T lymphocyte infiltration by Chiara Cencioni, Silvia Malatesta, Virginia Vigiano Benedetti, Valerio Licursi, Livia Perfetto, Federica Conte, Danilo Ranieri, Armando Bartolazzi, Martina Kunkl, Loretta Tuosto, Alberto Larghi, Geny Piro, Antonio Agostini, Giampaolo Tortora, Vincenzo Corbo, Carmine Carbone, Francesco Spallotta

“…Pancreatic neoplastic lesions evolve within a dense desmoplastic stroma, consisting in abundant extracellular matrix settled by cancer associated fibroblasts (CAFs). …”
Get full text

Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications by Colin D. Paul, Chris Yankaskas, Pradip Shahi Thakuri, Brittany Balhouse, Shyanne Salen, Amber Bullock, Sylvia Beam, Anthony Chatman, Sybelle Djikeng, Xiaoyu Jenny Yang, Garrett Wong, Isha Dey, Spencer Holmes, Abigail Dockey, Lindsay Bailey-Steinitz, Lina Zheng, Weizhong Li, Vivek Chandra, Jakhan Nguyen, Jason Sharp, Erik Willems, Mark Kennedy, Matthew R. Dallas, David Kuninger

“…Additionally, OncoPro medium was compatible with both embedded culture in extracellular matrix and growth in a suspension format for facile culture and scale up. …”
Get full text

Studying breast cancer lung metastasis using a multi-compartment microfluidic device with a mimetic tumor-stroma interaction model by Bahareh Zarin, Laleh Rafiee, Sorosh Abdollahi, Maryam Vatani, Mohsen Hassani, Amir Sanati-Nezhad, Shaghayegh Haghjooy Javanmard

“…Materials and methods: To model the primary tumor microenvironment, breast cancer cells (MCF7) and cancer-associated fibroblasts (CAFs) were co-cultured within the tumor compartment of the microfluidic chip while normal lung fibroblasts (NLFs) were seeded in a different compartment, as the secondary tumor site, separated from the tumor compartment via a Matrigel™ layer resembling the extracellular matrix. Results: The cytotoxic effect of β4 integrin blockade on cancer cells gradually increased after 48 and 72 h of co-culture. …”
Get full text

TEAD1‐Mediated Trans‐Differentiation of Vascular Smooth Muscle Cells into Fibroblast‐Like Cells Contributes to the Stabilization and Repair of Disrupted Atherosclerotic Plaques... by Ming Zhai, Zhijun Lei, Yefei Shi, Jiayun Shi, Yanxi Zeng, Shiyu Gong, Weixia Jian, Jianhui Zhuang, Qing Yu, Mark W. Feinberg, Wenhui Peng

“…Thus, this study illustrated that TEAD1 played a critical role in promoting the trans‐differentiation of VSMCs into fibroblast‐like cells and subsequent extracellular matrix production through the Wnt4/β‐Catenin pathway. …”
Get full text

Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen by Chizuka Obara, Kazuya Takizawa, Kenichi Tomiyama, Masaharu Hazawa, Ai Saotome-Nakamura, Takaya Gotoh, Takeshi Yasuda, Katsushi Tajima

“…Our results suggest that extracellular matrix components such as gelatin biases generated iMSC differentiation potential towards adipose or bone tissue in their derivation process via up- or downregulation of these master genes.…”
Get full text

Investigation of the Mechanism Underlying Calcium Dobesilate-Mediated Improvement of Endothelial Dysfunction and Inflammation Caused by High Glucose by Yijun Zhou, Chaojun Qi, Shu Li, Xinghua Shao, Zhaohui Ni

“…After PTX3 gene silencing, the urine protein and renal function of db/db mice were ameliorated, the glomerular extracellular matrix was decreased, and the expression of p-eNOS/eNOS was increased. …”
Get full text

Gallic acid protects intervertebral disc cells from ferroptosis and alleviates intervertebral disc degeneration by regulating key factors of oxidative stress by Zaishi Zhu, Zeling Huang, Chaofeng Zhang, Bo Xu, Hua Chen, Shuai Pei, Baofei Zhang, Lishi Jie, Xiaoqing Shi, Yujiang Liu, Yuwei Li, Yuwei Li, Xiaofeng Shen, Xiaofeng Shen

“…However, the role of GA in IDD ferroptosis remains unclear.MethodsThis study explored the pathological mechanism of GA in IDD in relation to ferroptosis: (1) to identify ferroptosis-related targets for GA treatment of IDD using network pharmacology and molecular docking technology, (2) to evaluate the therapeutic effect of GA in an IDD rat model and changes in ferroptosis-related targets, (3) to investigate the changes of oxidative stress and lipid peroxidation products in NP cells after GA intervention, and (4) to study the changes of ferroptosis-related proteins and iron ions in cells and mitochondria after GA intervention.ResultsExperimental results confirmed that GA can treat IDD by reducing the degradation of extracellular matrix (ECM) and pathological changes in IDD. …”
Get full text