Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification by Long Chen, Yi Xu, Lele Zhou, Ding Ma, Rong Zhang, Yifan Liu, Xianqiang Mi

“…Here, an ultra-sensitive single-cell sorting platform has been developed by integrating the FADS technology with Tetramer-HCR-EvaGreen (THE) fluorescence signal amplification. The THE system produced much higher fluorescence signal than that of the single Tetramer or Tetramer-HCR signal amplification. …”
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Assessment for Melting Temperature Measurement of Nucleic Acid by HRM by Jing Wang, Xiaoming Pan, Xingguo Liang

“…Both insufficiency and excessiveness of EvaGreen were found to give rise to a little bit higher Tm, showing that the proportion of dye should be considered for precise Tm measurement of nucleic acids. …”
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Cohesion policy and metropolitan governance: innovation in stakeholders collaboration practices in Lisbon by Margarida Queirós, Luís Balula, Mário Vale

“…Focusing on the Lisbon metropolitan area, which faces fragmented institutional structures and governance challenges, this research highlights the role of Cohesion Policy through Integrated Territorial Investments (ITI) in fostering innovation and collaboration among stakeholders. The EVA (Green and Blue Corridor) project in the Lisbon Metropolitan Area exemplifies the role of EU funding in promoting cooperation and governance innovation. …”
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<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci by E. A. Anisimova, N. A. Fakhrutdinov, D. A. Mirgazov, E. A. Dodonova, I. A. Elizarova, M. E. Gorbunova, N. I. Khammadov, L. I. Zainullin, K. A. Osyanin

“…To detect the differences between the strains, their PCR product melting points were measured in the presence of the EvaGreen (Sintol, Russia) intercalating dye. For SNP detection, a PCR assay with double TaqMan probes was applied. …”
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Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apcmin/+ mice from wildtype mice. by Yuting Sun, Tingyu Zhou, Silin Ye, Effie Yin Tung Lau, Yao Zeng, Jessie Qiaoyi Liang

“…This discrimination ability of these assays was unaffected by the use of different intercalating dyes (SYBR Green I or EvaGreen). Dual-probe qPCR assays were developed to simultaneously detect mutant and wildtype alleles using differently labelled probes. …”
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