Evaluation of twin-arginine translocation substrate proteins as potential antigen candidates for serodiagnosis of brucellosis by Yao Wu, Yao Wu, Xin Yan, Mingjun Sun, Xiaohan Guo, Jiaqi Li, Xiangxiang Sun, Xiangxiang Sun, Mengda Liu, Mengda Liu, Haobo Zhang, Haobo Zhang, Wenlong Nan, Weixing Shao, Fangkun Wang, Xiaoxu Fan, Xiaoxu Fan, Shufang Sun

“…Both the combined protein group and YxeI displayed no cross-reactivity with rabbit serum immunized against Yersinia enterocolitica O9, Escherichia coli O157:H7, Mycobacterium tuberculosis, Vibrio cholerae, Legionella, and Salmonella, indicating relatively good specificity.ConclusionThe findings of this study suggest that Tat substrate proteins serve as promising candidate antigens with significant potential value for the clinical diagnosis of brucellosis.…”
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Development of a Recombinant Fusion Vaccine Candidate Against Lethal <i>Clostridium botulinum</i> Neurotoxin Types A and B by Eun-Sun Choi, Seong-Wook Pyo, So-Hyeon Kim, Jun-Ho Jeon, Gi-Eun Rhie, Mi-Ran Yun, Hwajung Yi, Yoon-Seok Chung

“…Methods: We combined the HC_C domains of botulinum neurotoxin type A (BoNT/A) and botulinum neurotoxin type B (BoNT/B) in <i>Escherichia coli</i> to produce a recombinant protein (rHC_CB-L-HC_CArHCcB) that offers dual protection against both toxins by inhibiting their receptor binding. …”
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Akkermansia muciniphila identified as key strain to alleviate gut barrier injury through Wnt signaling pathway by Xin Ma, Meng Li, Yuanyuan Zhang, Tingting Xu, Xinchen Zhou, Mengqi Qian, Zhiren Yang, Xinyan Han

“…The aim of this research was to explore the effect of FMT on antibiotic-induced microbiome-disordered (AIMD) models infected with enterotoxigenic Escherichia coli (ETEC). We used piglet, mouse, and intestinal organoid models to explore the protective effects and mechanisms of FMT on ETEC infection. …”
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Antimicrobial Activities of Polysaccharide-Rich Extracts from the Irish Seaweed <i>Alaria esculenta</i>, Generated Using Green and Conventional Extraction Technologies, Against Foo... by Ailbhe McGurrin, Rahel Suchintita Das, Arturo B. Soro, Julie Maguire, Noelia Flórez Fernández, Herminia Dominguez, Maria Dolores Torres, Brijesh K. Tiwari, Marco Garcia-Vaquero

“…This study investigates the antimicrobial activity of compounds produced by the Irish seaweed <i>Alaria esculenta</i> against <i>Escherichia coli</i> and <i>Listeria innocua</i>, bacterial species which are relevant for food safety. …”
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Prevalence and Molecular Characterization of Carbapenemase-Producing Multidrug-Resistant Bacteria in Diabetic Foot Ulcer Infections by Mohd Saleem, Soha Abdallah Moursi, Tahani Nasser Almofeed Altamimi, Mohammed Salem Alharbi, Alwaleed Mohammad Alaskar, Sahar Adly Hassan Hammam, Ehab Rakha, Ozair Ilyas Syed Muhammad, Hamoud Abdulmohsin Almalaq, Metab Nasser Alshammari, Azharuddin Sajid Syed Khaja

“…The key identified pathogens included <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>, and <i>Escherichia coli</i>. The most commonly detected carbapenemase genes were <i>blaKPC</i> (27.6%) and <i>blaNDM</i> (24.1%). …”
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Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System by Eulalia de la Torre, Rocío Colello, Nora Lía Padola, Analía Etcheverría, Edgardo Rodríguez, Fabián Amanto, María Ofelia Tapia, Alejandro Luis Soraci

“…The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. …”
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OXA β-lactamases from Acinetobacter spp. are membrane bound and secreted into outer membrane vesicles by Lucia Capodimonte, Fernando Teixeira Pinto Meireles, Guillermo Bahr, Robert A. Bonomo, Matteo Dal Peraro, Carolina López, Alejandro J. Vila

“…Moreover, OXA-loaded OMVs were able to protect A. baumannii, Escherichia coli, and Pseudomonas aeruginosa cells susceptible to piperacillin and imipenem. …”
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Microbiological Analysis Conducted on Raw Milk Collected During Official Sampling in Liguria (North-West Italy) over a Ten-Year Period (2014–2023) by Sara Antonia Chiarlone, Andrea Gori, Serena Ravetta, Andrea Armani, Lisa Guardone, Francesca Pedonese, Salvatore Bavetta, Caterina Fiannacca, Nicola Pussini, Cristiana Maurella, Elisabetta Razzuoli

“…., <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i> O157 were analysed to better characterise the associated risk for consumers. …”
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Prevalence of common diarrheagenic enterobacteriaceae in Iran (2000–2023): a systematic review and meta-analysis by Mozhgan Derakhshan-Sefidi, Fereshteh Eidy, Somayyeh Nadi-Ravandi, Sareh Bagheri-Josheghani, Maryam Mirfakhraei

“…Subgroup analysis revealed Shigella spp. had the highest prevalence at 18.0% (95% CI: 0.13–0.24; I²=99%), followed by diarrheagenic Escherichia coli at 11.0% (95% CI: 0.09–0.15; I²=97%), Salmonella spp. at 9.0% (95% CI: 0.05–0.17; I²=99%), and Yersinia spp. at 2.0% (95% CI: 0.00-0.10; I²=94%). …”
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Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient by Yongjin Hu, Rong Tang, Shanshan Jin, Jiahao Guan, Xiaoxiao Meng, Zengpeijie Dan, Ruilan Wang, Hong-Yu Ou, Jian Lu

“…The carbapenem-resistant plasmid p2-KP3762–1 was found to transfer within species, from CRKP to hypervirulent KPRJF293HA, carbapenem-sensitive KP KP3657 and Escherichia coli C600 at a frequency of (1.19 ± 1.58) ×10−6, (1.09 ± 1.38) ×10−7 and (10.9 ± 9.53) ×10−6 respectively, resulting in the dissemination of carbapenem resistance genes. …”
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Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system by Sarah Hollingshead, Gareth McVicker, Maria R. Nielsen, YuGeng Zhang, Giulia Pilla, Rebekah A. Jones, Jonathan C. Thomas, Sarah E. H. Johansen, Rachel M. Exley, Ditlev E. Brodersen, Christoph M. Tang

“…Of note, mutations that confer tolerance to antibiotics in Escherichia coli also map to the DNA-binding domain of VapBC encoded by the chromosomally integrated F plasmid. …”
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Antimicrobial drug use and its association with antimicrobial resistance in fecal commensals from cows on California dairies by Essam M. Abdelfattah, Essam M. Abdelfattah, Pius S. Ekong, Emmanuel Okello, Emmanuel Okello, Tapakorn Chamchoy, Betsy M. Karle, Randi A. Black, Wagdy ElAshmawy, Wagdy ElAshmawy, David Sheedy, Deniece R. Williams, Terry W. Lehenbauer, Terry W. Lehenbauer, Barbara A. Byrne, Sharif S. Aly, Sharif S. Aly

“…The current study objective was to investigate the risk factors associated with the isolation of antimicrobial-resistant Escherichia coli, Enterococcus spp., and Streptococcus spp. …”
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Cloning and sequencing of VP19-encoding gene of white spot syndrome virus from Situbondo’s isolate by Sahrul Alim, Dinamella Wahjuningrum, Muhamad Ali

“…This research conducted through several stages, DNA genome isolation of Penaeus monodon infected with WSSV, amplification of VP19-encoding gene, isolation of VP19-encoding gene, ligation of VP19-encoding gene into pGEM-T Easy vector, transformation to Escherichia coli, recombinant vector screening, vector isolation using mini preparation technique, vector verification, and sequencing. …”
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Estandarización de zimosán opsonizado como estímulo en la técnica de la 1,2,3-dihidrorrodamina para evaluar el estallido respiratorio del neutrófilo by Uriel Pérez-Blanco, Jenniffer Yissel Girón, Guillermo Juárez -Vega, María Jiménez, Carlos Sánchez, Ricardo Rioja, Sara Espinosa-Padilla, Lizbeth Blancas-Galicia

“…Sin embargo, para la detección de la oxidación mediada por EROS y p40phox, se requiere de otros estímulos como zimosán, Escherichia coli o Staphylococcus aureus. Objetivo. Estandarizar el estímulo con zimosán en la técnica de la 1,2,3-dihidrorrodamina para evaluar el estallido respiratorio del neutrófilo. …”
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Contaminant-degrading bacteria are super carriers of antibiotic resistance genes in municipal landfills: A metagenomics-based study by Fang-Zhou Gao, Wei-Li Jia, Bing Li, Min Zhang, Liang-Ying He, Hong Bai, You-Sheng Liu, Guang-Guo Ying

“…Klebsiella pneumoniae, Acinetobacter nosocomialis and Escherichia coli were abundant multidrug-resistant pathogenic species in raw leachate (∼10.2 % of total microbiomes), but they rarely carried contaminant-degradation genes. …”
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Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses by Mathias K.-M. Herzog, Audrey Peters, Nizar Shayya, Monica Cazzaniga, Kardokh Kaka Bra, Trisha Arora, Manja Barthel, Ersin Gül, Luca Maurer, Patrick Kiefer, Philipp Christen, Katharina Endhardt, Julia A. Vorholt, Gad Frankel, Markus M. Heimesaat, Stefan Bereswill, Cormac G. M. Gahan, Marcus J. Claesson, Xavier Domingo-Almenara, Wolf-Dietrich Hardt

“…., Listeria monocytogenes and enteropathogenic/enterohemorrhagic Escherichia coli (EPEC/EHEC) are leading causes of food-borne illness worldwide. …”
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Antimicrobial Resistance Surveillance of Skin and Soft Tissue Infections: Hospital-Wide Bacterial Species and Antibiograms to Inform Management at a Zonal Tertiary Hospital in Mwan... by Justine BN, Mushi MF, Silago V, Igembe Z, Muyombe J, Kishengena PP, Michael Jnr NS, Maganga MG, Massenga A, Tegete F, Massaga FA, Seni J

“…The commonest pathogens were Staphylococcus aureus (17.1%), Escherichia coli (17.1%), and K. pneumoniae (16.0%). The AMR phenotypes identified were methicillin resistant Staphylococcus aureus, 29.0%; Extended-spectrum beta lactamase producing Gram-negative bacteria, 47.3%; and carbapenem resistant Gram-negative bacteria, 12.9%. …”
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Thermoresponsive Gels with Rosemary Essential Oil: A Novel Topical Carrier for Antimicrobial Therapy and Drug Delivery Applications by Ludovic Everard Bejenaru, Adina-Elena Segneanu, Cornelia Bejenaru, Ionela Amalia Bradu, Titus Vlase, Dumitru-Daniel Herea, Marius Ciprian Văruţ, Roxana Maria Bălăşoiu, Andrei Biţă, Antonia Radu, George Dan Mogoşanu, Maria Viorica Ciocîlteu

“…Antimicrobial efficacy was assessed against common pathogens associated with skin infections, including <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Candida albicans</i>. The RoEO-PLGA-loaded gels demonstrated significant inhibitory effects, confirming their potential as effective carriers for controlled and localized drug delivery. …”
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Late-stage diversification strategy for the synthesis of peptide acids and amides using hydrazides by Shoko Tanaka, Mizuki Kanno, Yosuke Tashiro, Tetsuo Narumi, Nobuyuki Mase, Kohei Sato

“…The antimicrobial activity of modelin-5 derivatives synthesized from the corresponding hydrazides was evaluated by the colony count of Escherichia coli (E. coli) after treatment with the peptides. …”
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Confocal Microscopy for Detection of Intracellular Bacteria in Urine Samples of patients Suspected with Urinary Tract Infection: A Cross-sectional Observational Study by Bhagyashri Patil-Takbhate, Nilam Memane, Srikanth Tripathy, Nageswari Gandham, Shahzad Mirza

“…Introduction: Uropathogenic bacteria like Escherichia coli (UPEC) and Pseudomonas are the most common agents of Urinary Tract Infection (UTI). …”
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