Changes in Methylation Patterns of Tumor Suppressor Genes during Extended Human Embryonic Stem Cell Cultures by Kyung Min Kang, Jeoung Eun Lee, Ji Eun Park, Hyunjin Kim, Hee Yeon Jang, Minyeon Go, Dong Ryul Lee, Sung Han Shim

“…While studies on embryonic stem cells have been actively conducted, little is known about the epigenetic mechanisms in human embryonic stem cells (hESCs) in extended culture systems. …”
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Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens by Gopu Sriram, Vaishali Prakash Natu, Intekhab Islam, Xin Fu, Chaminda Jayampath Seneviratne, Kai Soo Tan, Tong Cao

“…Recently, the potential application of multipotent stem cells and pluripotent stem cells including human embryonic stem cells (hESCs) in periodontal regenerative therapy has been proposed. …”
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miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells by Zhong-Yan Chen, Fei Chen, Nan Cao, Zhi-Wen Zhou, Huang-Tian Yang

“…Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs). With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. …”
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Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis by Jane Carr-Wilkinson, Nilendran Prathalingam, Deepali Pal, Mohammad Moad, Natalie Lee, Aishwarya Sundaresh, Helen Forgham, Peter James, Mary Herbert, Majlinda Lako, Deborah A. Tweddle

“…Our aim was to optimise a method for the differentiation of human embryonic stem cells (hESCs) to sympathetic neuron-like cells (SN) to model normal human SNS development. …”
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Generation and analysis of mouse embryonic stem cells with knockout of the <i>Mcph1</i> (microcephalin) gene by A. M. Yunusova, A. V. Smirnov, T. A. Shnaider, I. E. Pristyazhnuk, S. Y. Korableva, N. R. Battulin

“…In this study, we generated several mouse embryonic stem cell (mESC) lines with knockout of the Mcph1 gene and analyzed their gene expression profile. …”
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Generation and Characterization of Erythroid Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells: An Overview by Kai-Hsin Chang, Halvard Bonig, Thalia Papayannopoulou

“…Because of the imbalance in the supply and demand of red blood cells (RBCs), especially for alloimmunized patients or patients with rare blood phenotypes, extensive research has been done to generate therapeutic quantities of mature RBCs from hematopoietic stem cells of various sources, such as bone marrow, peripheral blood, and cord blood. Since human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be maintained indefinitely in vitro, they represent potentially inexhaustible sources of donor-free RBCs. …”
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Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy by Xinxin Han, Liming Yu, Jie Ren, Min Wang, Zhongliang Liu, Xinyu Hu, Daiyu Hu, Yihong Chen, Li Chen, Ying Zhang, Yuehua Liu, Xiaoqing Zhang, Hua He, Zhengliang Gao

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p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation by Na Li, Zhaoyu Du, Yunxiang Li, Wenjing Xu, Yumei Yang, Haodong Peng, Tianxiang Song, Qihua Qin, Huining Lei, Jinlian Hua

“…Embryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. …”
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Laminin 521 Stabilizes the Pluripotency Expression Pattern of Human Embryonic Stem Cells Initially Derived on Feeder Cells by Halima Albalushi, Magdalena Kurek, Leif Karlsson, Luise Landreh, Kristín Rós Kjartansdóttir, Olle Söder, Outi Hovatta, Jan-Bernd Stukenborg

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Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production by Chloë Deelkens, Elly De Vlieghere, Mario Van Poucke, Masaki Kinoshita, Jeffrey Aalders, Lieven Thorrez, Bert Devriendt, Ann Van Soom, Luc Peelman, Björn Menten, Catharina De Schauwer, Jolanda van Hengel

Subjects: “…Bovine embryonic stem cells…”
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Dynamic Proteomic Analysis of Pancreatic Mesenchyme Reveals Novel Factors That Enhance Human Embryonic Stem Cell to Pancreatic Cell Differentiation by Holger A. Russ, Limor Landsman, Christopher L. Moss, Roger Higdon, Renee L. Greer, Kelly Kaihara, Randy Salamon, Eugene Kolker, Matthias Hebrok

“…Current approaches in human embryonic stem cell (hESC) to pancreatic beta cell differentiation have largely been based on knowledge gained from developmental studies of the epithelial pancreas, while the potential roles of other supporting tissue compartments have not been fully explored. …”
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F4/80+ Host Macrophages Are a Barrier to Murine Embryonic Stem Cell-Derived Hematopoietic Progenitor Engraftment In Vivo by Heather L. Thompson, Nico van Rooijen, Bryce T. McLelland, Jennifer O. Manilay

“…Understanding how embryonic stem cells and their derivatives interact with the adult host immune system is critical to developing their therapeutic potential. …”
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Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments by Abdullah A. Alarfaj, Abdurahman H. Hirad, Murugan A. Munusamy, S. Suresh Kumar, Akon Higuchi

“…Vitronectin‐derived peptides with glycine joint nanosegments (GCGG) were conjugated onto poly (vinyl alcohol‐co‐itaconic acid) hydrogels via PEG joint nanosegments, and human embryonic stem cells (hESCs) were cultivated on these hydrogels. hESCs could successfully be cultivated on hydrogels while maintaining their pluripotency and differentiation potential to differentiate into cells that are induced from three germ layers in vitro and in vivo, where only a 50 μg/ml ECM‐derived peptide concentration was used when the PEG joint nanosegments were introduced into peptides that were grafted onto hydrogel surfaces. …”
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Development of Hematopoietic and Endothelial Cells from Human Embryonic Stem Cells: Lessons from the Studies using Mouse as a Model by Anna Jezierski, Albert Swedani, Lisheng Wang

“…The current progress using the human embryonic stem cell (hESC) model system has provided much insight into the early origins of the hematopoietic and endothelial lineages, particularly the elusive hemangioblast. …”
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Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells by Romain Barbet, Isabelle Peiffer, Antoinette Hatzfeld, Pierre Charbord, Jacques A. Hatzfeld

“…Using sensitive and quantitative TaqMan Low Density Arrays (TLDA) methodology, we compared the expression of 381 genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs (hES-MSCs), and hMSCs. …”
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Chromatin Structure and U2AF1-RS1 Gene Expression in Embryonic Stem Cells following RA-Induced Apoptosis by N. Andollo, M.D. Boyano, M. Garcia-Sanz, A. Asumendi, M.M. Zalduendo, J. Arechaga

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Effects of the auxin-dependent degradation of the cohesin and condensin complexes on the repair of distant DNA double-strand breaks in mouse embryonic stem cells by A. V. Smirnov, A. S. Ryzhkova, A. M. Yunusova

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Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation by Sergio Mora-Castilla, Juan R. Tejedo, Rafael Tapia-Limonchi, Irene Díaz, Ana B. Hitos, Gladys M. Cahuana, Abdelkrim Hmadcha, Franz Martín, Bernat Soria, Francisco J. Bedoya

“…We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. …”
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Mitofusin2 Promotes β Cell Maturation from Mouse Embryonic Stem Cells via Sirt3/Idh2 Activation by Li Lu, Li Zhitao, Cui Nannan, Huang Mingzhu, Hu Xiaoping, Hong Dongsheng, Pan Zongfu, Lu Xiaoyang

“…We demonstrated that Mfn2 promoted embryonic stem cell-derived β cell maturation via the Sirt3/Idh2 pathway, providing new insights into β cell development. …”
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Morphometric Analysis of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes: Determining the Maturation State of a Population by Quantifying Parameters in Individual Cells by Harvey Y. S. Chan, Wendy Keung, Ronald A. Li, Andrew L. Miller, Sarah E. Webb

“…Quantitative methods were established to determine the level of maturation of human embryonic stem cell-derived ventricular cardiomyocytes (hESC-vCMs) that were treated with different metabolic stimulants (i.e., isoproterenol and oleic acid) during early differentiation. …”
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