Degenerated vision, altered lipid metabolism, and expanded chemoreceptor repertoires enable Lindaspio polybranchiata to thrive in deep-sea cold seeps by Yujie Yan, Inge Seim, Yang Guo, Xupeng Chi, Zhaoshan Zhong, Dantong Wang, Mengna Li, Haining Wang, Huan Zhang, Minxiao Wang, Chaolun Li

“…As a pioneer species, L. polybranchiata has an expanded repertoire of genes encoding potential chemoreceptor proteins, including ionotropic receptors (IRs) and gustatory receptor-like receptors (GRLs). …”
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Antimicrobial resistance in clinical <I>Escherichia coli</I> isolates obtained from animals by M. N. Isakova, O. V. Sokolova, N. A. Bezborodova, A. S. Krivonogova, A. G. Isaeva, V. D. Zubareva

“…Absence of VIM carbapenemase-encoding gene in the DNA of the recovered isolates and a low level of phenotypic resistance (10.22% of isolates from cervical swabs) can be one of the reasons for recommending first-line carbapenems as antibiotics of choice to treat animal diseases associated with Escherichia coli, along with fluoroquinolones as reserve antimicrobials. …”
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Newcastle Disease Virus Displaying an Ectodomain of Middle East Respiratory Syndrome Coronavirus Spike Protein Elicited Robust Humoral and Cellular Immunity in Mice by Jaturawitt Prasopsiri, Kanjana Srisutthisamphan, Benjamas Liwnaree, Juggragarn Jengarn, Jarin Kramyu, Payuda Hansoongnern, Papon Muangsanit, Nathiphat Tanwattana, Challika Kaewborisuth, Suttipun Sungsuwan, Anan Jongkaewwattana, Nanchaya Wanasen

“…Methods: We codon-optimized the gene encoding the ectodomain of the MERS-CoV spike protein and incorporated modifications at the S1/S2 and S2’ cleavage sites, along with a proline substitution at residues V1060-L1061. …”
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Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang by Wenqiang Wang, Hao Wen, Qiang Jin, Wenjun Yu, Gen Li, Minyu Wu, Hongjin Bai, Lirong Shen, Cuiyun Wu

“…Ten key enzymes including ACCase, LACS6, LACS8, SAD, FAD2, FAD3, LPAAT1, DGAT2, PDAT2, and PLC encoded by 19 genes were highly associated with lipid biosynthesis. …”
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Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells by Carl D. Richards, Laura Izakelian, Anisha Dubey, Grace Zhang, Steven Wong, Karen Kwofie, Aatif Qureshi, Fernando Botelho

“…Responses were assessed in BALB/c mouse lung at day 7 of transient overexpression using endotracheally administered adenovirus encoding OSM (AdOSM) or empty vector (AdDel70). Whole lung extracts showed induction of IL-33 mRNA (>20-fold) and protein (10-fold increase in immunoblots) by AdOSM relative to AdDel70. …”
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Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in Plasmodium berghei by Samhita Das, Tanaya Unhale, Carine Marinach, Belsy del Carmen Valeriano Alegria, Camille Roux, Hélène Madry, Badreddine Mohand Oumoussa, Rogerio Amino, Shiroh Iwanaga, Sylvie Briquet, Olivier Silvie

“…To illustrate the versatility of this new tool, we conditionally knocked out the essential gene encoding the claudin-like apicomplexan micronemal protein (CLAMP) in P. berghei and confirmed the role of CLAMP during invasion of erythrocytes.…”
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Current molecular understanding of central nervous system schwannomas by Takahiro Tsuchiya, Satoru Miyawaki, Yu Teranishi, Kenta Ohara, Yudai Hirano, Shotaro Ogawa, Seiei Torazawa, Yu Sakai, Hiroki Hongo, Hideaki Ono, Nobuhito Saito

“…The development of these tumors is primarily linked to mutations in the NF2 gene. Merlin, the protein encoded by NF2, is integral to several signaling pathways, including Ras/Raf/MEK/ERK, PI3K/Akt/mTORC1, Wnt/β-catenin, and the Hippo pathway. …”
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SF1-specific deletion of the energy sensor AMPKγ2 induces obesity by Óscar Freire-Agulleiro, Ánxela Estévez-Salguero, Vitor Ferreira, Cassie Lynn Holleman, Julia García-Currás, Ismael González-García, Rubén Nogueiras, Manuel Tena-Sempere, Cristina García-Cáceres, Carlos Diéguez, Miguel López

“…Objective: AMP-activated protein kinase (AMPK) is a heterotrimer complex consisting of a catalytic α subunit (α1, α2) with a serine/threonine kinase domain, and two regulatory subunits, β (β1, β2) and γ (γ1, γ2, γ3), encoded by different genes. In the hypothalamus, AMPK plays a crucial role in regulating energy balance, including feeding, energy expenditure, peripheral glucose and lipid metabolism. …”
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Epigenetic and Neurological Impairments Associated with Early Life Exposure to Persistent Organic Pollutants by Nathalie Grova, Henri Schroeder, Jean-Luc Olivier, Jonathan D. Turner

“…Together, these first two “hits” produce a quiescent or latent phenotype, most probably encoded in the epigenome, which has become susceptible to a third environmental “hit” in later life. …”
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Convergent origin and accelerated evolution of vesicle-associated RhoGAP proteins in two unrelated parasitoid wasps by Colinet, Dominique, Cavigliasso, Fanny, Leobold, Matthieu, Pichon, Apolline, Urbach, Serge, Cazes, Dominique, Poullet, Marine, Belghazi, Maya, Volkoff, Anne-Nathalie, Drezen, Jean-Michel, Gatti, Jean-Luc, Poirié, Marylène

“…We show that the gene encoding the cellular RacGAP1 is at the origin of the virulent RhoGAP families found in Leptopilina sp. and V. canescens. …”
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Analysis of expression and transport activity of sugar transporter gene HpSWEET7 from red pitaya by ZHENG Qianming, YAN Shuang, XIE Pu, WANG Honglin, MA Yuhua

“…[Results] The ORF of HpSWEET7 was 771 bp, encoding 256 amino acid residues. HpSWEET7 contained two MtN3_saliva (Pfam No. …”
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Characterization of chemosensory genes in the subterranean pest Gryllotalpa Orientalis based on genome assembly and transcriptome comparison by Amna Chudhary, De-Long Guan, Yandi Xu, Tao Jiang, Lulu Yang, Mengyang Chen, Muhammad Salabat Khan, Wenhui Zhu, Sheng-Quan Xu

“…Results We present a draft genome of G. orientalis at the scaffold level, spanning 2.94 Gb and comprising 10,497 scaffolds. This assembly encodes 19,155 protein-coding genes, including 158 chemosensory genes: 30 odorant receptors (ORs), 64 ionotropic receptors (IRs), ten gustatory receptors (GRs), 28 odorant-binding proteins (OBPs), 25 chemosensory proteins (CSPs), and a single sensory neuron membrane protein (SNMP). …”
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Single-cell RNA sequencing of circulating immune cells supports inhibition of TNFAIP3 and NFKBIA translation as psoriatic arthritis biomarkers by Ameth N. Garrido, Rohan Machhar, Omar F. Cruz-Correa, Darshini Ganatra, Sarah Q. Crome, Sarah Q. Crome, Joan Wither, Joan Wither, Joan Wither, Igor Jurisica, Igor Jurisica, Igor Jurisica, Dafna D. Gladman, Dafna D. Gladman, Dafna D. Gladman

“…Immunoblotting of the proteins encoded in these genes (IκBα and A20, respectively) showed higher levels in PsA CD4+ T cells compared to HC. …”
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Knockdown of hexokinase 2 results in a decreased expression level of the glycolytic enzymes PFKP, BPGM, and GPI in RKO cell line by M. S. Fedorova, I. Y. Karpova, A. V. Lipatova, E. A. Pudova, Z. G. Guvatova, D. V. Kochetkov, A. V. Chaika, B. Y. Alekseev, M. V. Kiseleva, A. D. Kaprin, A. V. Kudryavtseva, A. V.  Snezhkina

“…Quantitative expression analysis of 15 genes (GAPDH, ADPGK, ALDOA, ENO3, PFKL, PGK1, PGAM1, PKM2, ENO1, PDK1, PDK3, PFKP, ENO2, GPI, and BPGM), encoding the key glycolysis enzymes, as well as HIF1A gene was carried out in a modified RKO cell line, which constantly expresses the short hairpin RNA (shRNA) for the inhibition of hexokinase 2. …”
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Multiscale Adaptively Spatial Feature Fusion Network for Spacecraft Component Recognition by Wuxia Zhang, Xiaoxiao Shao, Chao Mei, Xiaoying Pan, Xiaoqiang Lu

“…The MASFFN comprises a spatial attention-aware encoder (SAE) and a multiscale adaptively spatial feature fusion-based decoder (Multi-ASFFD). …”
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Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital by Tania Nawfal Dagher, Linda Hadjadj, Fadi Bittar, Fadi Fenianos, Elias Abdo, Jean-Marc Rolain, Charbel Al-Bayssari

“…Antibiotic susceptibility testing was carried out using the disk diffusion method and carbapenemase-encoding genes were searched for using standard polymerase chain reaction. …”
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Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation by Yiping Wan, Jiannan Zhang, Xiao Zhang, Jiliang He, Ningkun Shi, Yuanyou Li, Juan Li, Yajun Wang

“…Our results showed that: (1) cC5H11ORF96 cDNA encodes a 120 amino acids protein, which shares high sequence identity with that of birds, mammals, reptiles, frogs and fish; (2) cC5H11ORF96 has a fully conserved RFKTQP motif and high proportion of serine, indicating its multiple potential phosphorylation sites; (3) cC5H11ORF96 is widely expressed in various chicken tissues, with high expression levels in the parathyroid gland, adrenal gland, and pituitary; (4) glucocorticoids (GCs) and stress significantly upregulate C5H11ORF96 mRNA and protein expression in the chicken pituitary and hypothalamus, suggesting its involvement in regulating stress response by influencing the negative feedback of GCs on the HPA axis in chickens. …”
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Purpurin suppresses Salmonella invasion of host cells by reducing the secretion of T3SS-1 effector proteins by Zhenxu Shi, Zhimin Guo, Siqi Li, Chenxiao Jiang, Jianfeng Wang, Xuming Deng, Hongtao Liu, Jiazhang Qiu

“…Salmonella pathogenicity island 1 (SPI-1), encoding type three secretion system 1 (T3SS-1), serves as a crucial virulence factor for the invasion of ST into host cells, making it an ideal target for screening anti-Salmonella virulence drugs. …”
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A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways in <i>Komagataella phaffii</i> by Albert Fina, Sílvia Àvila-Cabré, Enrique Vázquez-Pereira, Joan Albiol, Pau Ferrer

“…Co-overexpression of genes encoding a NADPH-dependent redox shuttle from <i>Saccharomyces cerevisiae</i> (<i>IDP2</i>/<i>IDP3</i>) significantly increased 3-HP yields across all substrates, whereas peroxisomal targeting of the <i>S. cerevisiae</i> NADH kinase Pos5 failed to improve 3-HP production. …”
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Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum) by Yi Liu, Chao Bian, Ka Yan Ma, Yexin Yang, Yuanyuan Wang, Chao Liu, Guochang Ouyang, Meng Xu, Jinhui Sun, Changwei Shao, Jiehu Chen, Qiong Shi, Xidong Mu

“…., around 26-kb upstream of foxl2 gene (encoding forkhead box L2). Its strong interaction with the neighboring foxl2 on the same chromosome suggests foxl2 as a candidate sex-related gene in silver arowana. …”
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