Genome-Wide Analyses Reveal the Roles of CCT Genes as Regulators of Abiotic Stress Responses in <i>Citrullus lanatus</i> by Yajie Hong, Mengsha Li, Wona Ding, Jun Shi, Zishuo Zheng, Nailin Xing, Qiuping Li

“…Functional diversity was also evident among CCT family genes within a given species as well as among species attributable to changes in the structural features and expression patterns of the genes and the encoded proteins.…”
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Effective Resistivity in Relativistic Reconnection: A Prescription Based on Fully Kinetic Simulations by Abigail Moran, Lorenzo Sironi, Aviad Levis, Bart Ripperda, Elias R. Most, Sebastiaan Selvi

“…The guide field dependence is encoded in α and p , which we fit to PIC data. This resistivity formulation—which relies only on single-fluid MHD quantities—successfully reproduces the spatial structure and strength of nonideal electric fields and thus provides a promising strategy for enhancing the reconnection rate in resistive MHD simulations.…”
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Genomic, Evolutionary, and Pathogenic Characterization of a New <i>Polerovirus</i> in Traditional Chinese Medicine <i>Viola philippica</i> by Yuanling Chen, Gaoxiang Chen, Jiaping Yu, Yali Zhou, Shifang Fei, Haorong Chen, Jianxiang Wu, Shuai Fu

“…VPPV possesses a linear, positive-sense, single-stranded RNA genome consisting of 5535 nucleotides (nt) and encodes seven highly overlapping open reading frames (ORFs). …”
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NaP-TRAP reveals the regulatory grammar in 5’UTR-mediated translation regulation during zebrafish development by Ethan C. Strayer, Srikar Krishna, Haejeong Lee, Charles Vejnar, Nils Neuenkirchen, Amit Gupta, Jean-Denis Beaudoin, Antonio J. Giraldez

“…Abstract The cis-regulatory elements encoded in an mRNA determine its stability and translational output. …”
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Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections by Yuehan Ni, Jiamiao Wu, Fengqi Liu, Yating Yi, Xiangjiao Meng, Xiang Gao, Luyi Xiao, Weiwei Zhou, Zexi Chen, Peng Chu, Dan Xing, Ye Yuan, Donghui Ding, Ge Shen, Min Yang, Ronjie Wu, Ling Wang, Luiza Martins Nascentes Melo, Sien Lin, Xiaoguang Cheng, Gang Li, Alpaslan Tasdogan, Jessalyn M. Ubellacker, Hu Zhao, Shentong Fang, Bo Shen

“…Completed within approximately 12 days, this process yields highly transparent tissues that maintain genetically encoded or antibody-stained fluorescent signals. The bone hemisections are compatible with diverse antibody labeling strategies. …”
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Production and characterization of nervous necrosis virus vaccine antigens in wild type and CRISPR/Cas9 genome edited Nicotiana benthamiana and edible crop lettuce Lactuca sativa by Iva Andrasevic, Hang Su, Espen Rimstad, Jihong Liu Clarke

“…In the present study, we report: (1) selection and design of recombinant plasmids encoding the capsid proteins (CPs) of two genotypes of NNV, red-spotted grouper nervous necrosis virus (RGNNV) and striped jack nervous necrosis virus (SJNNV); (2) design and optimization of plasmids for transient expression of NNV vaccine antigens in wild type Nicotiana benthamiana, CRISPR/Cas9 genome edited Nicotiana benthamiana and Lactuca sativa; (3) test of different Agrobacterium strains (LBA4404 and AGL1) for effective production of NNVCPs; (4) the expression patterns of NNVCPs over time post infiltration for different plants and cultivars; (5) successful production of NNV antigens in N. benthamiana lines and lettuce cultivars, indicating the potential of the plants as antigen producers in the development of a plant-based vaccine against VNN.…”
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SensorDBSCAN: Semi-Supervised Active Learning Powered Method for Anomaly Detection and Diagnosis by Petr Ivanov, Maria Shtark, Alexander Kozhevnikov, Maksim Golyadkin, Dmitry Botov, Ilya Makarov

“…The proposed approach combines a transformer-based encoder trained with a triplet-based contrastive learning objective and the classical density-based clustering algorithm DBSCAN, enabling strong feature extraction, efficient and interpretable feature space organization and simple clustering algorithm. …”
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Altered Forebrain Functional Connectivity and Neurotransmission in a Kinase-Inactive Mouse Model of Autism by Shiyu Tang PhD, Elizabeth M. Powell PhD, Wenjun Zhu MS, Fu-Sun Lo MD, Reha S. Erzurumlu PhD, Su Xu PhD

“…MET , the gene encoding the tyrosine kinase receptor for hepatocyte growth factor, is a susceptibility gene for autism spectrum disorder (ASD). …”
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Splicing and expression dynamics of SR genes in hot pepper (Capsicum annuum): regulatory diversity and conservation under stress by Lin Li, Yueqin Zhang, Rui Zhang, Rui Zhang, Xiangtao Cen, Yongxiang Huang, Yongxiang Huang, Hanqiao Hu, Hanqiao Hu, Xingyu Jiang, Xingyu Jiang, Yu Ling, Yu Ling

“…In this study, we identified and characterized 23 genes encoding serine/arginine-rich (SR) protein in hot pepper (Capsicum annuum), named CaSR here. …”
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Multiscale guided attention network for optic disc segmentation of retinal images by A Z M Ehtesham Chowdhury, Andrew Mehnert, Graham Mann, William H. Morgan, Ferdous Sohel

“…The method is named multiscale guided attention network (MSGANet-OD), comprising encoders for extracting multiscale features and decoders for constructing segmentation maps from the extracted features. …”
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Mutation-Specific Phenotypes in hiPSC-Derived Cardiomyocytes Carrying Either Myosin-Binding Protein C Or α-Tropomyosin Mutation for Hypertrophic Cardiomyopathy by Marisa Ojala, Chandra Prajapati, Risto-Pekka Pölönen, Kristiina Rajala, Mari Pekkanen-Mattila, Jyrki Rasku, Kim Larsson, Katriina Aalto-Setälä

“…To date, over 1400 mutations have been linked to HCM, mostly in genes encoding for sarcomeric proteins. However, the pathophysiological mechanisms of the disease are still largely unknown. …”
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Open-Label Fosmetpantotenate, a Phosphopantothenate Replacement Therapy in a Single Patient with Atypical PKAN by Yiolanda-Panayiota Christou, George A. Tanteles, Elena Kkolou, Annita Ormiston, Kostas Konstantopoulos, Maria Beconi, Randall D. Marshall, Horacio Plotkin, Kleopas A. Kleopa

“…PKAN is caused by biallelic PANK2 mutations, a gene that encodes pantothenate kinase 2, a regulatory enzyme in coenzyme A biosynthesis. …”
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Association of PTPN22 Haplotypes (−1123G>C/+1858C>T) with Rheumatoid Arthritis in Western Mexican Population by Yeniley Ruiz-Noa, Jorge Ramón Padilla-Gutiérrez, Jorge Hernández-Bello, Claudia Azucena Palafox-Sánchez, Yeminia Valle, Edith Oregón-Romero, Ana Laura Pereira-Suárez, Ana Guilaisne Bernard-Medina, José Francisco Muñoz-Valle

“…Two polymorphic variants (−1123G>C and +1858C>T) at PTPN22 gene that encodes this protein have been associated with autoimmune disorders and found in strong linkage disequilibrium in Caucasian population. …”
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Genome Mining and Characterization of Two Novel <i>Lacticaseibacillus rhamnosus</i> Probiotic Candidates with Bile Salt Hydrolase Activity by Gianluigi Agolino, Marianna Cristofolini, Amanda Vaccalluzzo, Davide Tagliazucchi, Alice Cattivelli, Alessandra Pino, Cinzia Caggia, Lisa Solieri, Cinzia Lucia Randazzo

“…Each strain harbors a single <i>bsh</i> gene, encoding a C-N amide hydrolase, which conserved the essential residues required in the BSH core site. …”
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Immune Dysfunction in Rett Syndrome Patients Revealed by High Levels of Serum Anti-N(Glc) IgM Antibody Fraction by Anna Maria Papini, Francesca Nuti, Feliciana Real-Fernandez, Giada Rossi, Caterina Tiberi, Giuseppina Sabatino, Shashank Pandey, Silvia Leoncini, Cinzia Signorini, Alessandra Pecorelli, Roberto Guerranti, Solange Lavielle, Lucia Ciccoli, Paolo Rovero, Claudio De Felice, Joussef Hayek

“…Rett syndrome (RTT), a neurodevelopmental disorder affecting exclusively (99%) female infants, is associated with loss-of-function mutations in the gene encoding methyl-CpG binding protein 2 (MECP2) and, more rarely, cyclin-dependent kinase-like 5 (CDKL5) and forkhead box protein G1 (FOXG1). …”
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Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ by HAO Lanlan, WANG Hong, ZHANG Fan, ZHANG Xuebing, YANG Yang

“…[Results] (1) Peach PpSAT4 gene with a sequence of 864 bp was cloned, encoding a protein with a molecular weight of 30.49 kD and an isoelectric point of 6.70. …”
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The biogenesis, regulation and functions of transitive siRNA in plants by Tan Huijun, Liu Yuelin, Guo Hongwei

“…As core molecules of RNAi, various sRNAs are encoded in the plant genome or derived from invading RNA molecules, and their biogenesis depends on distinct genetic pathways. …”
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Deterministic genetic barcoding for multiplexed behavioral and single-cell transcriptomic studies by Jorge Blanco Mendana, Margaret Donovan, Lindsey Gengelbach O'Brien, Benjamin Auch, John Garbe, Daryl M Gohl

“…This method, called Targeted Genetically-Encoded Multiplexing (TaG-EM), involves inserting a DNA barcode just upstream of the polyadenylation site in a Gal4-inducible UAS-GFP construct so that the barcode sequence can be read out during single-cell sequencing, labeling a cell population of interest. …”
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Scent of COVID-19: Whole-Genome Sequencing Analysis Reveals the Role of <i>ACE2</i>, <i>IFI44</i>, and <i>NDUFAF4</i> in Long-Lasting Olfactory Dysfunction by Beatrice Spedicati, Alessandro Pecori, Maria Pina Concas, Aurora Santin, Romina Ruberto, Giuseppe Giovanni Nardone, Andrea D’Alessandro, Giancarlo Tirelli, Paolo Boscolo-Rizzo, Giorgia Girotto

“…Considering the physiological role of the proteins encoded by these genes, it can be hypothesized that a reduced expression of <i>ACE2</i> may be associated with a protracted and severe inflammatory response in the olfactory epithelium, thus worsening patients’ smell abilities. …”
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Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells by Yuanyuan Mei, Xiaoting Sun, Shi-Ying Huang, Xiaowen Wu, Kuo-Ting Ho, Liming Lu, Chaoxiang Chen, Jian Li, Jingwen Liu, Guiling Li

“…And 48 h overexpression of miR-22-3p targeting cardiolipin synthase 1 ( CRLS1 ) gene, which encodes a mitochondrial phospholipid synthase, showed a similar regulatory effect. …”
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