Mechanisms of blaIMP-4 dissemination across diverse carbapenem-resistant clinical isolates by Ying Fu, Faye C. Morris, Stephanie C. Pereira, Xenia Kostoulias, Yan Jiang, Callum Vidor, Galain Williams, Yogi Srikhanta, Nenad Macesic, Yunsong Yu, Dena Lyras, Anton Y. Peleg

“…IncH12A plasmids and K. pneumoniae IncC displayed higher transfer efficiencies than other plasmids examined in this study when using the recipient E. coli strain J53 (with conjugation efficiencies of 1.17×10−2 to 5.02×10−5, P < 0.001). …”
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Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> by Rebeca Dias dos Santos, Breno Noronha Matos, Daniel Oliveira Freire, Franklyn Santos da Silva, Bruno Alcântara do Prado, Karolina Oliveira Gomes, Marta Oliveira de Araújo, Carla Azevedo Bilac, Letícia Fernandes Silva Rodrigues, Izabel Cristina Rodrigues da Silva, Lívia Cristina Lira de Sá Barreto, Claudio Augusto Gomes da Camara, Marcilio Martins de Moraes, Guilherme Martins Gelfuso, Daniela Castilho Orsi

“…The time–growth inhibition assay also suggests the effectiveness of the NE against the tested pathogens <i>S. aureus</i> and <i>E. coli</i>, highlighting its potential as a novel alternative therapeutic agent.…”
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Peptide platform for 3D-printed Ti implants with synergistic antibacterial and osteogenic functions to enhance osseointegration by Chenying Cui, Yifan Zhao, Jingyu Yan, Ziyang Bai, Guning Wang, Yingyu Liu, Yurong Xu, Lihong Zhou, Kaifang Zhang, Yanling Mi, Binbin Zhang, Xiuping Wu, Bing Li

“…In both in vitro and in vivo infected bone defect models, 3DTi-NFP implants demonstrated excellent biocompatibility and achieved over 90 % bactericidal efficiency against S. aureus and E. coli. The implants reduced bacterial colonization while enhancing adhesion, proliferation, and differentiation of bone marrow mesenchymal stem cells (BMSCs), significantly upregulating osteogenic genes and protein expression. …”
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Epidemiology and Resistance Profiles of Bacteria Isolated From Blood Samples in Septic Patients at Emergency Department Admission: A 6-Year Single Centre Retrospective Analysis Fro... by Valeria Cento, Sara Carloni, Riccardo Sarti, Linda Bussini, Zian Asif, Paola Morelli, Francesco De Fazio, Federica Maria Tordato, Maddalena Casana, Debora Mondatore, Antonio Desai, Elena Generali, Nicola Pugliese, Elena Costantini, Massimo Vanoni, Maurizio Cecconi, Stefano Aliberti, Giorgio Da Rin, Erminia Casari, Michele Bartoletti, Antonio Voza

“…Results: Antibiotic-susceptible Escherichia coli (29.5%) was the most common isolate, including extended-spectrum beta-lactamase (ESBL)-producing strains (11.3%), followed by methicillin-susceptible Staphylococcus aureus (MSSA, 8.4%). BSIs due to E. coli were more frequent in patients >60 years (43.9% vs. 27.3%, P < 0.001) and associated with ESBL production (OR = 2.202, P = 0.031) and urosepsis (OR = 1.688, P = 0.006). …”
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Investigating novel Streptomyces bacteriophage endolysins as potential antimicrobial agents by Jindanuch Maneekul, Amanda Chiaha, Rachel Hughes, Faith Labry, Joshua Saito, Matthew Almendares, Brenda N. Banda, Leslie Lopez, Nyeomi McGaskey, Melizza Miranda, Jenil Rana, Brandon R. Zadeh, Lee E. Hughes

“…The killing assay suggests that Tb42lys has a broader range (E. coli, P. putida, A. baylyi and K. aerogenes). While Nb26lys better attacks Gram-negative than -positive bacteria, LL35lys can only reduce the growth of the Gram-positive ESKAPE strains but does so effectively with a low MIC90 of 2 µg/mL. …”
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آنالیز گسترده ژنوم، تفسیر و بررسی بیان ژن‌های VDAC در گل رز چینی by گل محمد اشرفی, مصطفی خوشحال سرمست, جین لیو

“…قطعه تکثیر شده از روی cDNA به کمک واکنش زنجیره‌ای پلیمراز با استفاده از حامل همسانه‌سازی PENTER ، جداسازی و در باکتری E-Coli تکثیر و سپس توالی‌یابی شد. از همان پایگاه داده، 1500 جفت باز از راه‌انداز 7 ژن VDAC گل رز چینی برای آنالیز جدا شد و شناسایی نواحی اتصال عوامل رونویسی با استفاده از سایت PlantCARE انجام شد. …”
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Dynamic association of antimicrobial resistance in urinary isolates of Escherichia coli and Klebsiella pneumoniae between primary care and hospital settings in the Netherlands (200... by Evelyn Pamela Martínez, Annelies Verbon, Annelot F. Schoffelen, Wieke Altorf-van der Kuil, Joost van Rosmalen, James W.T. Cohen Stuart, Damian C. Melles, Karin van Dijk, Ali Alzubaidy, Maarten Scholing, Sacha D. Kuil, Gert J. Blaauw, Wieke Altorf - van der Kuil, Saskia M. Bierman, Sabine C. de Greeff, Sonja R. Groenendijk, Rudy Hertroys, Jos C.M. Monen, Daan W. Notermans, Jan Polman, Wouter J. van den Reek, Caroline Schneeberger-van der Linden, Annelot F. Schoffelen, Feline Velthuis, Cornelia C.H. Wielders, Britt J. de Wit, Rony E. Zoetigheid, Wouter van den Bijllaardt, Elise M. Kraan, Michiel B. Haeseker, Júlia M. da Silva, Eefje de Jong, Boulos Maraha, Arjanne J. van Griethuysen, Bastiaan B. Wintermans, Marijke J.C.A. van Trijp, Anouk E. Muller, Man-Chi Wong, Alewijn Ott, Erik Bathoorn, Mariëtte Lokate, Jan Sinnige, Damian C. Melles, Nienke Plantinga, Nicole H.M. Renders, Julia W. Dorigo-Zetsma, Leendert J. Bakker, Wim Ang, Karola Waar, Martha T. van der Beek, Maurine A. Leversteijn-van Hall, Suzan P. van Mens, Erik Schaftenaar, Marrigje H. Nabuurs-Franssen, Ianthe Maat, Patrick D.J. Sturm, Bram M.W. Diederen, Lonneke G.M. Bode, David S.Y. Ong, Michiel van Rijn, Sander Dinant, Martijn den Reijer, Dick W. van Dam, Els I.G.B. de Brauwer, Robbert G. Bentvelsen, Anton G.M. Buiting, Anne L.M. Vlek, Melanie de Graaf, Annet Troelstra, Arjan R. Jansz, Maurits P.A. van Meer, Janneke de Vries, Julian D. Machiels

“…Depending on the bacterium–antibiotic combination, a 1% increase of AMR in E. coli and K. pneumoniae in primary care leads to an increase of AMR in hospital settings ranging from 0.10% to 0.40%. …”
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کلونینگ و توالی یابی ژن p- انسولین جدا شده از گیاه دارویی Momordica charantia و تحلیل بیوانفورماتیکی ساختار سه بعدی پروتئین... by مریم رحیمی, مجید عزیزی, احد یامچی, مجید شهبازی

“…پس از خالص سازی باند مربوط به ژن p-انسولین ، واکنش لیگاسیون ژن هدف در داخل تی وکتور و ترانسفورماسیون آن به داخل میزبان E. coli صورت گرفت که نتایج مثبت موید انجام موفق کلونینگ بود. …”
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Comprehensive epidemiological evaluation of ruminant brucellosis and associated risk factors in some Egyptian Governorates by Nesreen Allam Tantawy Allam, Mahinour Ezzeldin Abdelsalam, Hend I. Elsharkawy, Mai Mohamed Kandil, Amany Mohamed Mohamed Mohamed, Fatma Ali, Mohamed A. Gebely, Safaa Y. Nour, Doaa Sedky, Mona Ebrahim Hussien Abd El-Gawad, Hoda M. Zaki, Nazek Al-Gallas, Amal M. Aboelmaaty, Mona Mohamed Sobhy, Nagwa Sayed Ata, Marwa Salah Abdel-Hamid, Ghada A. Badawy

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