High prevalence of fecal carriage of extended-spectrum beta-lactamase producing Enterobacterales among patients with urinary tract infections in rural Tanzania by Magreth Erick Macha, Magreth Erick Macha, Magreth Erick Macha, Weihong Qi, Salome N. Seiffert, Anja Bösch, Philipp Kohler, Honorathy Msami Urassa, Sabine Haller, Erin West, Maja Weisser Rohacek, Maja Weisser Rohacek, Maja Weisser Rohacek, Baharak Babouee Flury, Baharak Babouee Flury, Baharak Babouee Flury, Baharak Babouee Flury

“…Fecal ESBL-PE colonization was detected in 70.9% of patients, predominantly E. coli (62.8%) and K. pneumoniae (33.0%). Whole-genome sequencing identified diverse phylogroups and sequence types, with CTX-M-15 being the most common ESBL gene. …”
Get full text

Green synthesis of nano-copper oxide using Sargassum sp. functionalized in cellulose acetate membrane for dye adsorption by U. Yanuhar, H. Suryanto, M. Amin, J.S. Binoj, I. Casuarina

“…Nano-copper oxide was introduced to bacterial acetate produced from pineapple peel waste to make a composite membrane and observed the antibacterial activity against E.coli and S.aureus, and dye adsorption to Methylene Blue, Malachite Green, Metanil Yellow, and Congo Red for 60 minutes contact time.FINDINGS: Nano-copper oxide is successfully synthesized using macroalgae Sargassum sp. proved by wavelength of ultra violet-visual spectroscopy results at 300 nanometer and energy dispersive spectroscopy analysis that denotes the presence of copper at 0.93 and 8.04 kilo electron volt. …”
Get full text

Biogenic Silver Nanoparticles of Clinacanthus nutans as Antioxidant with Antimicrobial and Cytotoxic Effects by Hock Ing Chiu, Che Nurul Azieyan Che Mood, Nur Nadhirah Mohamad Zain, Muggundha Raoov Ramachandran, Noorfatimah Yahaya, Nik Nur Syazni Nik Mohamed Kamal, Wai Hau Tung, Yoke Keong Yong, Chee Keong Lee, Vuanghao Lim

“…The inhibition results showed the highest activity against Staphylococcus aureus (S. aureus) with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition was against Escherichia coli (E. coli) with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in the disc diffusion method. …”
Get full text

Two-component system GrpP/GrpQ promotes pathogenicity of uropathogenic Escherichia coli CFT073 by upregulating type 1 fimbria by Xueping Li, Yu Pang, Lingyan Jiang, Le Liu, Jiarui Zhou, Chen Jin, Qian Wang, Hongmin Sun, Qing Li, Zhen Chen, Jingliang Qin, Jianwei Mu, Bin Liu, Qiyue Zhang, Yutao Liu, Lu Feng, Lei Wang

“…This study reported a previously uncharacterized two-component system (TCS) GrpP/GrpQ that directly activates type 1 fimbria expression to promote UPEC invasion and therefore pathogenicity in response to D-serine present in the host urine. grpP/grpQ mutation severely impaired UPEC invasion of BECs and decreased the bacterial burden and formation of intracellular bacterial communities in mouse bladders during acute UTI. grpP/grpQ is widely present in UPEC genomes but rarely in other E. coli genomes, suggesting that this TCS specifically contributes to UPEC evolution. …”
Get full text

Designing a multi-epitope vaccine candidate against human rhinovirus C utilizing immunoinformatics approach by Tajul Islam Mamun, Tajul Islam Mamun, Md. Ahad Ali, Md. Nazmul Hosen, Jillur Rahman, Md. Anwarul Islam, Md. Golam Akib, Kamruz Zaman, Md. Masudur Rahman, Md. Masudur Rahman, Ferdaus Mohd Altaf Hossain, Ferdaus Mohd Altaf Hossain, Samir Ibenmoussa, Mohammed Bourhia, Turki M. Dawoud

“…Furthermore, in silico cloning and sequence optimization ensured efficient expression in E. coli, with a Codon Adaptation Index of 0.99 and GC content of 54.58%. …”
Get full text

Natural Food Colorant Obtained from Wild <i>Berberis vulgaris</i> L. by Ultrasound-Assisted Extraction: Optimization and Characterization by Erika N. Vega, Lorena González-Zamorano, Elena Cebadera, Lillian Barros, Tânia C. S. P. Pires, Adriana K. Molina, Tayse F. F. da Silveira, Guillermo Vidal-Diez de Ulzurrun, Javier Tardío, Montaña Cámara, Virginia Fernández-Ruiz, Patricia Morales

“…Additionally, both BVFF and BVE presented significant antioxidant, antibacterial, and antifungal activity, especially in the case of BVE, which inhibited the growth of several foodborne bacteria and fungi and even showed bactericidal capacity against most of the tested bacteria, particularly against <i>E. cloacae</i>, <i>E. coli</i>, <i>P. aeruginosa</i>, and <i>B. cereus</i>. …”
Get full text

Antimicrobial Activities of Polysaccharide-Rich Extracts from the Irish Seaweed <i>Alaria esculenta</i>, Generated Using Green and Conventional Extraction Technologies, Against Foo... by Ailbhe McGurrin, Rahel Suchintita Das, Arturo B. Soro, Julie Maguire, Noelia Flórez Fernández, Herminia Dominguez, Maria Dolores Torres, Brijesh K. Tiwari, Marco Garcia-Vaquero

“…Extracts had antimicrobial activity against <i>E. coli</i> and featured minimum inhibitory concentration (MIC) values of 6.25 mg/mL (in the case of the extract prepared with UAE) and 12.5 mg/mL (in the case of the extracts prepared with MAE, UMAE, and conventional maceration). …”
Get full text

Rapid inference of antibiotic susceptibility phenotype of uropathogens using metagenomic sequencing with neighbor typing by Amanda C. Carroll, Leanne Mortimer, Hiren Ghosh, Sandra Reuter, Hajo Grundmann, Karel Brinda, William P. Hanage, Angel Li, Aimee Paterson, Andrew Purssell, Ashley Rooney, Noelle R. Yee, Bryan Coburn, Shola Able-Thomas, Martin Antonio, Allison McGeer, Derek R. MacFadden

“…We used neighbor typing to predict MLST and susceptibility phenotype of E. coli (64/80) and Klebsiella spp. (16/80) from urine samples. …”
Get full text

Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study by Yaqin Peng, Ruijie Xie, Yifeng Luo, Yifeng Luo, Yifeng Luo, Penghao Guo, Zhongwen Wu, Yili Chen, Pingjuan Liu, Jiankai Deng, Bin Huang, Kang Liao

“…(AUC: 0.282, 95% CI: 0.000-0.778) had little predictive value for the corresponding culture-proven BSIs.ConclusionOur results indicate that the multiplex ddPCR is a promising platform as a complementary add-on to conventional BC. The DNA loads of E. coli and K. pneumoniae present excellent predictive value for the corresponding BSIs. …”
Get full text

Microbiological Analysis Conducted on Raw Milk Collected During Official Sampling in Liguria (North-West Italy) over a Ten-Year Period (2014–2023) by Sara Antonia Chiarlone, Andrea Gori, Serena Ravetta, Andrea Armani, Lisa Guardone, Francesca Pedonese, Salvatore Bavetta, Caterina Fiannacca, Nicola Pussini, Cristiana Maurella, Elisabetta Razzuoli

“…<i>Listeria monocytogenes</i>, <i>S. aureus</i>, and <i>E. coli</i> O157 were never responsible for non-compliances. …”
Get full text

Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system by Sarah Hollingshead, Gareth McVicker, Maria R. Nielsen, YuGeng Zhang, Giulia Pilla, Rebekah A. Jones, Jonathan C. Thomas, Sarah E. H. Johansen, Rachel M. Exley, Ditlev E. Brodersen, Christoph M. Tang

“…We found that vapBC mutations in E. coli that lead to antibiotic tolerance (a precursor to resistance) also operate by the same mechanism (i.e., generating VapB that is prone to cleavage); free VapC during tolerance will arrest bacterial growth and prevent susceptibility to antibiotics. …”
Get full text

Microwave-Assisted Synthesized ZnO@APTES Quantum Dots Exhibits Potent Antibacterial Efficacy Against Methicillin-Resistant Staphylococcus aureus Without Inducing Resistance by Du F, Niu J, Hong Y, Fang X, Geng Z, Liu J, Xu F, Liu T, Chen Q, Zhai J, Miao B, Liu S, Zhang Y, Chen Z

“…The minimum inhibitory concentrations (MIC) were determined to be 64 μg mL− 1 for Escherichia coli (E. coli) and 32 μg mL− 1 for MRSA. The ZnO@APTES QDs showed significant inhibition of MRSA biofilm formation and effectively disrupted mature biofilms. …”
Get full text

Risk factors associated with mortality and pathogen characteristics of bloodstream infection-induced severe sepsis in the pediatric intensive care unit: a retrospective cohort stud... by Jian Chen, Jian Chen, Haixin Huang, Ruichen Zhang, Yueqiang Fu, Chunmei Jing

“…The detection rates of extended-spectrum cephalosporin resistance (ECR) and fluoroquinolone resistance (FQR) in Escherichia coli (E. coli) were higher than those in Klebsiella pneumoniae.ConclusionIn the PICU, higher mortality was observed in children with BSI-induced severe sepsis who presented with elevated pSOFA scores and low P/F values. …”
Get full text

Preparation Methods and Perceived Risk of Foodborne Illness Among Consumers of Prepackaged Frozen Vegetables – United States, September 2022 by Michelle Canning, Michael Ablan, Tamara N. Crawford, Amanda Conrad, Alexandra Busbee, Misha Robyn, Katherine E. Marshall

“…A third (34.1% [95% CI = 32.2–35.9%]) of respondents agreed that frozen vegetables can be contaminated with germs (like Salmonella, E. coli, and Listeria), with a greater proportion of people with cancer disagreeing compared to those without cancer (32.5% vs 23.4%, p = 0.041). …”
Get full text

Eco-friendly synthesis of silver nanoparticles: Enhancing optimization reaction, characterization, and antimicrobial properties with Lantana camara from geothermal area by Pati Kemala, Rinaldi Idroes, Khairan Khairan, Muliadi Ramli, Binawati Ginting, Zuchra Helwani, Rifki Aulia, Ghazi Mauer Idroes, Muhammad Yusuf, Rustam Efendi

“…The antimicrobial testing was conducted against Gram-positive bacterium (S. aureus), Gram-negative bacterium (E. coli), and the fungus (C. albicans). The phytochemicals analysis revealed that the L. camara leaf extract contains flavonoids, phenolics, saponins, tannins, and steroids, lacking alkaloids and terpenoids, with total phenolic and flavonoid contents of 11.94 mg (GAE/g) and 6.70 mg (QE/g), respectively. …”
Get full text

Oral-Delivery Lactococcus lactis expressing cherry fusion lactoferrin peptides against infection of avian pathogenic Escherichia coli in chickens by Zhuoran Li, Xueying Wang, Dianzhong Zheng, Fuzhen Han, Yue Li, Han Zhou, Jiaxuan Li, Wen Cui, Yanping Jiang, Xiaona Wang, Weichun Xie, Lijie Tang

“…Our investigation focused on the impact of LL-EFLmC strain on the gut microbiota composition and avian pathogenic E. coli O78 challenge. Our findings indicate that LL-EFLmC exhibits inhibitory effects against APEC-O78 and Staphylococcus aureus CVCC26003 (S. aureus CVCC26003) in vitro. …”
Get full text

Evaluating the health risk of probiotic supplements from the perspective of antimicrobial resistance by Qiwen Tian, Hailv Ye, Xuan Zhou, Junyi Wang, Lifang Zhang, Wenxuan Sun, Chenxin Duan, Minyu Fan, Wei Zhou, Chuyun Bi, Qiong Ye, Aloysius Wong

“…Consistently, ARGs conferring resistance to streptomycin (aadA) and erythromycin [erm(B)−1] were detected in E. coli and S. aureus transconjugants, respectively, after co-incubation with streptomycin-adapted probiotics on Caco-2 cells. aadA and erm(B)−1 were both detected in E. faecalis transconjugant after the same co-incubation on HCT-116 cells. …”
Get full text

Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves by Letiele Bruck de Souza, Amanda Leitão Gindri, Thainara de Andrade Fortes, Thais Felli Kubiça, Jefferson Enderle, Rafael Roehrs, Sidnei Moura e Silva, Vanusa Manfredini, Elton Luís Gasparotto Denardin

“…Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. …”
Get full text

Confocal Microscopy for Detection of Intracellular Bacteria in Urine Samples of patients Suspected with Urinary Tract Infection: A Cross-sectional Observational Study by Bhagyashri Patil-Takbhate, Nilam Memane, Srikanth Tripathy, Nageswari Gandham, Shahzad Mirza

“…While the culture report showed 23 (25.0%) were E. coli, 29 (31.5%) were polymicrobial flora and 29 (31.05%) were negative for culture. …”
Get full text

The synergetic effect of selenium or zinc oxide nanoparticles with chromium on mitigating thermal stress for sustainable production and improving antioxidant capacity and inflammat... by I. T. El-Ratel, K. H. El-Kholy, S. M. Elgmmal, S. F. Fouda, A.-K. E. Abdel-Khalek, M. A. Hassan, M. M. Azzam, M. Alagawany, A. Lestingi

“…The caecal activity was increased, while the <i>Escherichia coli</i> (<i>E. coli</i>) count decreased considerably in all treatments compared to the control. …”
Get full text